KlpnC.19962.a

DNA polymerase IV

CENTER ID: KlpnC.19962.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.19962.a.B1.GE44400 Full length(KlpnC.19962.a) 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.945
UniProt: A0A0H3GN39

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKIIHVDMD CFFAAVEMRD NPALRDIPLA IGGSRVQRGV ISTANYPARK FGVRSAMPTA TALKLCPHLT LLPGRFDAYK EASNHIREIF SRYTSRIEPL SLDEAYLDVS DSEHCHGSAT LIAQEIRQTI ERELRLTASA GVAPVKFLAK IASDMNKPNG QFVIAPHQVA EFVRALPLAK IPGVGKVSAA KLENMGLRTC GDVQNSDLAM LLKRFGKFGR ILWERSHGID EREIHNDRQR KSVGVERTLA EDIHEWPECE AIIENLYPEL ERRLAKVKPD LLIARQGIKL KFNDFQLTTQ EHVWPRLNKE DLIATAHKAW DERRGGRGVR LVGLHVTLLD PQLERQLLLG I
NT Sequence
ATGCGTAAAA TTATCCATGT CGATATGGAC TGTTTCTTTG CGGCGGTGGA GATGCGCGAC AACCCAGCGT TGCGCGATAT TCCCCTCGCG ATAGGCGGCA GTCGGGTACA ACGTGGCGTG ATTAGTACCG CGAACTATCC TGCGCGTAAG TTTGGCGTGC GCAGCGCCAT GCCGACGGCG ACGGCGCTCA AGCTGTGCCC GCACCTCACG CTGCTTCCTG GCCGCTTTGA TGCCTACAAA GAAGCCTCGA ACCATATCCG CGAAATTTTC TCCCGCTATA CCTCGCGCAT TGAGCCGCTG TCGCTGGATG AAGCCTATCT TGACGTCAGC GACAGCGAGC ATTGTCACGG GTCAGCCACC CTGATAGCCC AGGAGATCCG CCAGACCATC GAACGGGAGC TGCGCCTCAC CGCCTCGGCG GGGGTGGCGC CGGTGAAGTT TCTCGCTAAA ATCGCTTCAG ATATGAATAA ACCCAACGGC CAGTTCGTTA TCGCTCCCCA TCAGGTGGCA GAGTTTGTGC GGGCGCTGCC GCTGGCGAAA ATACCCGGCG TCGGGAAGGT ATCGGCAGCG AAGCTGGAAA ATATGGGCCT GCGAACCTGT GGCGATGTGC AGAACAGCGA TCTGGCCATG CTGCTTAAGC GCTTCGGTAA ATTCGGGCGT ATTCTGTGGG AGCGCAGCCA CGGGATTGAT GAGCGGGAAA TTCATAACGA TCGGCAGCGT AAATCGGTGG GCGTGGAGCG CACCTTAGCG GAAGATATAC ATGAATGGCC GGAGTGCGAA GCGATTATCG AAAACCTCTA TCCCGAGCTG GAGAGGCGAC TGGCGAAGGT CAAACCTGAT CTGCTGATCG CCCGCCAGGG CATTAAACTG AAATTCAATG ATTTCCAGCT GACCACCCAG GAGCACGTCT GGCCGCGGCT GAACAAAGAG GATCTGATCG CCACCGCGCA CAAAGCCTGG GATGAGCGCC GCGGCGGACG GGGCGTGCGC CTGGTTGGGC TCCACGTGAC GTTGCTCGAT CCGCAGCTGG AAAGGCAGCT TTTATTAGGG ATT
Details for KlpnC.19962.a.B1.GE44400
HARVESTED ON: data unavailable
SEQUENCED ON: 7/22/2021
EXPECTED MW: 41kDa
OBSERVED MW: 81kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR KIIHVDMDCF FAAVEMRDNP ALRDIPLAIG GSRVQRGVIS TANYPARKFG VRSAMPTATA LKLCPHLTLL PGRFDAYKEA SNHIREIFSR YTSRIEPLSL DEAYLDVSDS EHCHGSATLI AQEIRQTIER ELRLTASAGV APVKFLAKIA SDMNKPNGQF VIAPHQVAEF VRALPLAKIP GVGKVSAAKL ENMGLRTCGD VQNSDLAMLL KRFGKFGRIL WERSHGIDER EIHNDRQRKS VGVERTLAED IHEWPECEAI IENLYPELER RLAKVKPDLL IARQGIKLKF NDFQLTTQEH VWPRLNKEDL IATAHKAWDE RRGGRGVRLV GLHVTLLDPQ LE
Validated NT Sequence
cctttccagc tgcggatcga gcaacgtcac gtggagccca accaggcgca cgccccgtcc gccgcggcgc tcatcccagg ctttgtgcgc ggtggcgatc agatcctctt tgttcagccg cggccagacg tgctcctggg tggtcagctg gaaatcattg aatttcagtt taatgccctg gcgggcgatc agcagatcag gtttgacctt cgccagtcgc ctctccagct cgggatagag gttttcgata atcgcttcgc actccggcca ttcatggata tcttccgcta aggtgcgctc cacgcccacc gatttacgct gccgatcgtt atgaatttcc cgctcatcaa tcccgtggct gcgctcccac agaatacgcc cgaatttacc gaagcgctta agcagcatgg ccagatcgct gttttgcaca tcgccacagg ttcgcaggcc catattttcc agcttcgctg ccgatacctt cccgacgccg ggtattttcg ccagcggcag cgcccgcaca aactctgcca cctgatgggg agcgataacg aactggccgt tgggtttatt catatctgaa gcgattttag cgagaaactt caccggcgcc acccccgccg aggcggtgag gcgcagctcc cgttcgatgg tctggcggat ctcctgggct atcagggtgg ctgacccgtg acaatgctcg ctgtcgctga cgtcaagata ggcttcatcc agcgacagcg gctcaatgcg cgaggtatag cgggagaaaa tttcgcggat atggttcgag gcttctttgt aggcatcgaa gcggccagga agcagcgtga ggtgcgggca cagcttgagc gccgtcgccg tcggcatggc gctgcgcacg ccaaacttac gcgcaggata gttcgcggta ctaatcacgc cacgttgtac ccgactgccg cctatcgcga ggggaatatc gcgcaacgct gggttgtcgc gcatctccac cgccgcaaag aaacagtcca tatcgacatg gataatttta cgcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMR KIIHVDMDCF FAAVEMRDNP ALRDIPLAIG GSRVQRGVIS TANYPARKFG VRSAMPTATA LKLCPHLTLL PGRFDAYKEA SNHIREIFSR YTSRIEPLSL DEAYLDVSDS EHCHGSATLI AQEIRQTIER ELRLTASAGV APVKFLAKIA SDMNKPNGQF VIAPHQVAEF VRALPLAKIP GVGKVSAAKL ENMGLRTCGD VQNSDLAMLL KRFGKFGRIL WERSHGIDER EIHNDRQRKS VGVERTLAED IHEWPECEAI IENLYPELER RLAKVKPDLL IARQGIKLKF NDFQLTTQEH VWPRLNKEDL IATAHKAWDE RRGGRGVRLV GLHVTLLDPQ LERQLLLGI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgta aaattatcca tgtcgatatg gactgtttct ttgcggcggt ggagatgcgc gacaacccag cgttgcgcga tattcccctc gcgataggcg gcagtcgggt acaacgtggc gtgattagta ccgcgaacta tcctgcgcgt aagtttggcg tgcgcagcgc catgccgacg gcgacggcgc tcaagctgtg cccgcacctc acgctgcttc ctggccgctt tgatgcctac aaagaagcct cgaaccatat ccgcgaaatt ttctcccgct atacctcgcg cattgagccg ctgtcgctgg atgaagccta tcttgacgtc agcgacagcg agcattgtca cgggtcagcc accctgatag cccaggagat ccgccagacc atcgaacggg agctgcgcct caccgcctcg gcgggggtgg cgccggtgaa gtttctcgct aaaatcgctt cagatatgaa taaacccaac ggccagttcg ttatcgctcc ccatcaggtg gcagagtttg tgcgggcgct gccgctggcg aaaatacccg gcgtcgggaa ggtatcggca gcgaagctgg aaaatatggg cctgcgaacc tgtggcgatg tgcagaacag cgatctggcc atgctgctta agcgcttcgg taaattcggg cgtattctgt gggagcgcag ccacgggatt gatgagcggg aaattcataa cgatcggcag cgtaaatcgg tgggcgtgga gcgcacctta gcggaagata tacatgaatg gccggagtgc gaagcgatta tcgaaaacct ctatcccgag ctggagaggc gactggcgaa ggtcaaacct gatctgctga tcgcccgcca gggcattaaa ctgaaattca atgatttcca gctgaccacc caggagcacg tctggccgcg gctgaacaaa gaggatctga tcgccaccgc gcacaaagcc tgggatgagc gccgcggcgg acggggcgtg cgcctggttg ggctccacgt gacgttgctc gatccgcagc tggaaaggca gcttttatta gggatttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t