KlpnC.18849.a

Valine--tRNA ligase (EC 6.1.1.9) (Valyl-tRNA synthetase) (ValRS)

CENTER ID: KlpnC.18849.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18849.a.A1.PW39322 full length 1 951

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.503
UniProt: A0A0H3GQW8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKTYNPQDI EQPLYEHWEK QGYFKPNGDE SQESFCIMIP PPNVTGSLHM GHAFQQTIMD TMIRYQRMQG KNTLWQAGTD HAGIATQMVV ERKIAAEEGK TRHDYGRDAF IDKIWQWKAE SGGTITRQMR RLGNSVDWER ERFTMDEGLS NAVKEVFVRL YKEDLIYRGK RLVNWDPKLR TAISDLEVEN RESKGSMWHI RYPLADGAKT ADGKDYLVVA TTRPETLLGD TGVAVNPEDP RYKDLIGKFV VLPLVNRRIP IVGDEHADME KGTGCVKITP AHDFNDYEVG RRHQLPMINI LTFDGDIRES AEVYDTKGNE SDVYSSEIPA EFQKLERFAA RKAVVAAVDA LGLLEEIKPH DLTVPYGDRG GVVIEPMLTD QWYVRADVLA KPAVEAVENG DIQFVPKQYE NMYFSWMRDI QDWCISRQLW WGHRIPAWYD NDGNVYVGRS EDEVRQENNL SADVALRQDE DVLDTWFSSA LWTFSTLGWP ENTDALRQFH PTSVMVSGFD IIFFWIARMI MMTMHFIKDE NGKPQVPFKT VYMTGLIRDD EGQKMSKSKG NVIDPLDMVD GITLPELLEK RTGNMMQPQL ADKIRKRTEK QFPNGIEPHG TDALRFTLAA LASTGRDINW DMKRLEGYRN FCNKLWNASR FVLMNTEDQD CGFNGGEMVL SLADRWIIAE FNHTVKAYRE ALDNFRFDIA AGILYEFTWN QFCDWYLELT KPVMNGGNEA ELRGTRHTLV TVLEGLLRLA HPIIPFITET IWQRVKAICG ITADTIMLQP FPQYDASQVD DAALADTEWL KQAIVAVRNI RAEMNIAPGK PLELLLRGCS KDAERRVNDN RSFLLNLARL ESITVLPADD KGPVSVTKIV DGAELLIPMA GLINKEDELA RLAKEVAKIE GEIGRIESKL ANEGFVARAP EAVIAKEREK LEGYAEAKAK LIEQQAVIAA L
NT Sequence
atggaaaaga catacaaccc acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctatt tcaaaccgaa tggtgatgaa agccaggaga gcttctgcat catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcatgcct tccagcaaac catcatggat accatgattc gctaccagcg catgcagggc aaaaacaccc tgtggcaggc cggtaccgac cacgcaggta tcgctaccca gatggtggtt gagcgtaaga ttgccgctga agaagggaaa acccgccacg actacggccg cgacgccttt atcgacaaaa tctggcagtg gaaagcggaa tccggcggca ccattacccg tcagatgcgc cgtctcggca actccgtgga ctgggagcgc gagcgcttca ccatggacga ggggctttcc aatgccgtga aagaagtctt tgttcgcctg tacaaagaag acctgatcta ccgcggcaaa cgcctggtta actgggaccc gaaactgcgc accgccattt ccgacctcga agtggaaaac cgcgaatcga aaggttccat gtggcacatc cgctacccgc tggccgatgg cgcgaaaacc gctgacggta aagattacct ggtggtcgcc accacccgtc cggaaaccct gctgggcgat accggcgtgg ccgttaaccc ggaagatccg cgctacaaag atctgattgg caaattcgtg gtgctgccgc tggttaaccg ccgcatcccg atcgtgggcg acgagcacgc cgacatggaa aaaggcaccg gctgcgtgaa gatcaccccg gcgcacgact ttaacgacta cgaagtgggt cgtcgtcacc agctgccgat gatcaacatc cttacctttg acggcgacat tcgcgaaagc gcggaagtct acgacaccaa aggcaatgaa tccgacgtct actccagcga gatcccggcg gagttccaga aactggaacg cttcgcggcg cgtaaagccg ttgttgcggc agttgacgcc ctcggcctgc tggaagagat caaaccgcac gacctgaccg tcccttacgg cgatcgcggc ggcgtggtga tcgagccgat gctgaccgac cagtggtacg tgcgcgcgga cgtgctggcc aagccagcag tcgaagccgt tgaaaacggt gatattcagt tcgtgccgaa gcagtacgaa aatatgtact tctcctggat gcgcgatatt caggactggt gtatctcccg tcagctgtgg tggggtcacc gcatcccggc atggtatgac aacgacggca acgtctacgt aggccgtagc gaagacgaag tgcgtcagga aaacaacctg agcgccgacg ttgccctgcg tcaggatgaa gacgttctcg atacctggtt ctcctccgcg ctgtggacct tctccaccct cggctggccg gaaaacaccg acgccctgcg tcagttccac ccgaccagcg tgatggtgtc cggcttcgac atcatcttct tctggatcgc ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaagc cgcaggtacc gtttaagacc gtctacatga ccggtctgat ccgcgatgac gaaggccaga agatgtccaa gtccaagggt aacgtcattg acccgctgga tatggttgac ggtatcaccc tgccggagct gctggaaaaa cgtaccggca acatgatgca gccgcagctg gcggataaaa tccgcaagcg taccgagaaa cagttcccga acggcatcga gccgcacggc accgacgccc tgcgcttcac cctggcggcg ctggcctcta ccggccgcga catcaactgg gatatgaagc gtctggaagg ttaccgtaac ttctgtaaca agctgtggaa cgccagccgc ttcgtgctga tgaacaccga agatcaggat tgcggcttca acggcggcga aatggtgctc tccctggccg accgctggat catcgcggaa ttcaaccaca ccgtgaaagc gtatcgcgaa gcgctggata acttccgctt cgatattgcc gccggcatcc tgtacgagtt cacctggaac cagttctgcg actggtacct ggagctgacc aagccggtta tgaacggtgg caacgaagcg gagctgcgcg ggactcgcca tactctggtc accgtgctgg aaggtctgct gcgcctggcg catccgatca ttccattcat taccgagacc atctggcagc gcgtgaaagc gatctgcggg atcaccgccg acaccatcat gctgcagccg ttcccgcagt acgatgcttc tcaggtggat gacgcggcgc tggccgacac cgaatggctg aagcaggcga tcgtcgccgt gcgtaacatc cgtgcggaaa tgaacatcgc tccgggcaaa ccgctggaac tgctactgcg cggctgcagc aaagatgccg agcgtcgtgt gaacgacaac cgcagcttcc tgctgaacct ggcgcgtctg gaaagcatta ccgtgctgcc tgccgatgat aaaggtccgg tctcggtgac caagattgtc gacggcgccg agctgctgat cccgatggcg ggcctcatca ataaagaaga tgagctggcg cgtctggcga aagaagtggc caaaatcgaa ggtgaaattg gccgcatcga gagcaagctg gccaacgaag gctttgtcgc ccgcgcgccg gaagcagtga tcgctaaaga gcgcgagaag ctggaaggct acgcggaagc gaaagcgaag ctgattgagc agcaggcggt gatcgccgcg ctgtga
Details for KlpnC.18849.a.A1.PW39322
PURIFICATION DATe: 10/1/2024
CONCENTRATION: 13.5mg/ml
OBSERVED MW: 111kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 30 mM Imidazole, 0.025% Azide, 1 mM TCEP *3C cleavage was performed on original protein stock to remove His-tag; only reverse IMAC was performed, no SEC since it was performed in the original purification.
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEKTYNPQD IEQPLYEHWE KQGYFKPNGD ESQESFCIMI PPPNVTGSLH MGHAFQQTIM DTMIRYQRMQ GKNTLWQAGT DHAGIATQMV VERKIAAEEG KTRHDYGRDA FIDKIWQWKA ESGGTITRQM RRLGNSVDWE RERFTMDEGL SNAVKEVFVR LYKEDLIYRG KRLVNWDPKL RTAISDLEVE NRESKGSMWH IRYPLADGAK TADGKDYLVV ATTRPETLLG DTGVAVNPED PRYKDLIGKF VVLPLVNRRI PIVGDEHADM EKGTGCVKIT PAHDFNDYEV GRRHQLPMIN ILTFDGDIRE SAEVYDTKGN ESDVYSSEIP AEFQKLERFA ARKAVVAAVD ALGLLEEIKP HDLTVPYGDR GGVVIEPMLT DQWYVRADVL AKPAVEAVEN GDIQFVPKQY ENMYFSWMRD IQDWCISRQL WWGHRIPAWY DNDGNVYVGR SEDEVRQENN LSADVALRQD EDVLDTWFSS ALWTFSTLGW PENTDALRQF HPTSVMVSGF DIIFFWIARM IMMTMHFIKD ENGKPQVPFK TVYMTGLIRD DEGQKMSKSK GNVIDPLDMV DGITLPELLE KRTGNMMQPQ LADKIRKRTE KQFPNGIEPH GTDALRFTLA ALASTGRDIN WDMKRLEGYR NFCNKLWNAS RFVLMNTEDQ DCGFNGGEMV LSLADRWIIA EFNHTVKAYR EALDNFRFDI AAGILYEFTW NQFCDWYLEL TKPVMNGGNE AELRGTRHTL VTVLEGLLRL AHPIIPFITE TIWQRVKAIC GITADTIMLQ PFPQYDASQV DDAALADTEW LKQAIVAVRN IRAEMNIAPG KPLELLLRGC SKDAERRVND NRSFLLNLAR LESITVLPAD DKGPVSVTKI VDGAELLIPM AGLINKEDEL ARLAKEVAKI EGEIGRIESK LANEGFVARA PEAVIAKERE KLEGYAEAKA KLIEQQAVIA AL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAAAGACA TACAACCCAC AAGATATCGA ACAGCCGCTT TACGAGCACT GGGAAAAGCA GGGCTATTTC AAACCGAATG GTGATGAAAG CCAGGAGAGC TTCTGCATCA TGATCCCGCC GCCGAACGTC ACCGGCAGTT TGCATATGGG TCATGCCTTC CAGCAAACCA TCATGGATAC CATGATTCGC TACCAGCGCA TGCAGGGCAA AAACACCCTG TGGCAGGCCG GTACCGACCA CGCAGGTATC GCTACCCAGA TGGTGGTTGA GCGTAAGATT GCCGCTGAAG AAGGGAAAAC CCGCCACGAC TACGGCCGCG ACGCCTTTAT CGACAAAATC TGGCAGTGGA AAGCGGAATC CGGCGGCACC ATTACCCGTC AGATGCGCCG TCTCGGCAAC TCCGTGGACT GGGAGCGCGA GCGCTTCACC ATGGACGAGG GGCTTTCCAA TGCCGTGAAA GAAGTCTTTG TTCGCCTGTA CAAAGAAGAC CTGATCTACC GCGGCAAACG CCTGGTTAAC TGGGACCCGA AACTGCGCAC CGCCATTTCC GACCTCGAAG TGGAAAACCG CGAATCGAAA GGTTCCATGT GGCACATCCG CTACCCGCTG GCCGATGGCG CGAAAACCGC TGACGGTAAA GATTACCTGG TGGTCGCCAC CACCCGTCCG GAAACCCTGC TGGGCGATAC CGGCGTGGCC GTTAACCCGG AAGATCCGCG CTACAAAGAT CTGATTGGCA AATTCGTGGT GCTGCCGCTG GTTAACCGCC GCATCCCGAT CGTGGGCGAC GAGCACGCCG ACATGGAAAA AGGCACCGGC TGCGTGAAGA TCACCCCGGC GCACGACTTT AACGACTACG AAGTGGGTCG TCGTCACCAG CTGCCGATGA TCAACATCCT TACCTTTGAC GGCGACATTC GCGAAAGCGC GGAAGTCTAC GACACCAAAG GCAATGAATC CGACGTCTAC TCCAGCGAGA TCCCGGCGGA GTTCCAGAAA CTGGAACGCT TCGCGGCGCG TAAAGCCGTT GTTGCGGCAG TTGACGCCCT CGGCCTGCTG GAAGAGATCA AACCGCACGA CCTGACCGTC CCTTACGGCG ATCGCGGCGG CGTGGTGATC GAGCCGATGC TGACCGACCA GTGGTACGTG CGCGCGGACG TGCTGGCCAA GCCAGCAGTC GAAGCCGTTG AAAACGGTGA TATTCAGTTC GTGCCGAAGC AGTACGAAAA TATGTACTTC TCCTGGATGC GCGATATTCA GGACTGGTGT ATCTCCCGTC AGCTGTGGTG GGGTCACCGC ATCCCGGCAT GGTATGACAA CGACGGCAAC GTCTACGTAG GCCGTAGCGA AGACGAAGTG CGTCAGGAAA ACAACCTGAG CGCCGACGTT GCCCTGCGTC AGGATGAAGA CGTTCTCGAT ACCTGGTTCT CCTCCGCGCT GTGGACCTTC TCCACCCTCG GCTGGCCGGA AAACACCGAC GCCCTGCGTC AGTTCCACCC GACCAGCGTG ATGGTGTCCG GCTTCGACAT CATCTTCTTC TGGATCGCCC GCATGATCAT GATGACCATG CACTTCATCA AAGATGAAAA TGGCAAGCCG CAGGTACCGT TTAAGACCGT CTACATGACC GGTCTGATCC GCGATGACGA AGGCCAGAAG ATGTCCAAGT CCAAGGGTAA CGTCATTGAC CCGCTGGATA TGGTTGACGG TATCACCCTG CCGGAGCTGC TGGAAAAACG TACCGGCAAC ATGATGCAGC CGCAGCTGGC GGATAAAATC CGCAAGCGTA CCGAGAAACA GTTCCCGAAC GGCATCGAGC CGCACGGCAC CGACGCCCTG CGCTTCACCC TGGCGGCGCT GGCCTCTACC GGCCGCGACA TCAACTGGGA TATGAAGCGT CTGGAAGGTT ACCGTAACTT CTGTAACAAG CTGTGGAACG CCAGCCGCTT CGTGCTGATG AACACCGAAG ATCAGGATTG CGGCTTCAAC GGCGGCGAAA TGGTGCTCTC CCTGGCCGAC CGCTGGATCA TCGCGGAATT CAACCACACC GTGAAAGCGT ATCGCGAAGC GCTGGATAAC TTCCGCTTCG ATATTGCCGC CGGCATCCTG TACGAGTTCA CCTGGAACCA GTTCTGCGAC TGGTACCTGG AGCTGACCAA GCCGGTTATG AACGGTGGCA ACGAAGCGGA GCTGCGCGGG ACTCGCCATA CTCTGGTCAC CGTGCTGGAA GGTCTGCTGC GCCTGGCGCA TCCGATCATT CCATTCATTA CCGAGACCAT CTGGCAGCGC GTGAAAGCGA TCTGCGGGAT CACCGCCGAC ACCATCATGC TGCAGCCGTT CCCGCAGTAC GATGCTTCTC AGGTGGATGA CGCGGCGCTG GCCGACACCG AATGGCTGAA GCAGGCGATC GTCGCCGTGC GTAACATCCG TGCGGAAATG AACATCGCTC CGGGCAAACC GCTGGAACTG CTACTGCGCG GCTGCAGCAA AGATGCCGAG CGTCGTGTGA ACGACAACCG CAGCTTCCTG CTGAACCTGG CGCGTCTGGA AAGCATTACC GTGCTGCCTG CCGATGATAA AGGTCCGGTC TCGGTGACCA AGATTGTCGA CGGCGCCGAG CTGCTGATCC CGATGGCGGG CCTCATCAAT AAAGAAGATG AGCTGGCGCG TCTGGCGAAA GAAGTGGCCA AAATCGAAGG TGAAATTGGC CGCATCGAGA GCAAGCTGGC CAACGAAGGC TTTGTCGCCC GCGCGCCGGA AGCAGTGATC GCTAAAGAGC GCGAGAAGCT GGAAGGCTAC GCGGAAGCGA AAGCGAAGCT GATTGAGCAG CAGGCGGTGA TCGCCGCGCT GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA