KlpnC.18708.a

Isoleucine--tRNA ligase (IleRS)

CENTER ID: KlpnC.18708.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18708.a.B1.GE44350 Full length(KlpnC.18708.a) 1 950
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18708.a.A3.PW39336 AlphaFold domain 13 950

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.711
UniProt: A0A0H3GRH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFIKPKYGTE NLMSDYKSTL NLPETGFPMR GDLAKREPGM LARWTDDDLY GIIRAAKKGK KTFILHDGPP YANGSIHIGH SVNKILKDII VKSKGLTGYD SPYVPGWDCH GLPIELKVEQ EYGKPGEKFT AAEFRAKCRE YAAEQIDGQR KDFIRLGVLG DWSHPYLTMD FKTEANIIRA LGKIIGNGHL HKGAKPVHWC VDCRSALAEA EVEYYDKTSP SIDVAFHAVD KAAVLAKFGV ADVNGPVSLV IWTTTPWTLP ANRAISLSPE FDYALVQVDG QALILAKDLV ESVMKRVGAT DYTILAAVQG SELELMRFKH PFLDFDVPAI LGDHVTLDAG TGAVHTAGGH GPDDYTISQK YGLEIANPVG PDGAYLPGTW PSLDGINVFK ANDIIVEMLR ERGALLHVEK LQHSYPCCWR HKSPIIFRAT PQWFVSMDQK GLRAQSLKEI KGVQWIPDWG QARIESMVAN RPDWCISRQR TWGVPMSLFV HKETHELHPR TLELMEEVAK RVEVDGIQAW WDLDSRDILG DDADSYEKVP DTLDVWFDSG STHSSVVDVR PEFAGHAADM YLEGSDQHRG WFMSSLMIST AMKGKAPYRQ VLTHGFTVDG QGRKMSKSIG NTVSPQDVMN KLGADILRLW VASTDYTGEM AVSDEILKRA ADSYRRIRNT ARFLLANLNG FDPVKDMVKP EEMVVLDRWA VGCAQAAQED ILKAYESYDF HEVVQRLMRF CSIEMGSFYL DIIKDRQYTA KADSVARRSC QTALFHIVEA LVRWMAPIMS FTADEIWGYL PGEREKYVFT GEWYEGLFGL ADDEAMNDDF WDELLKVRGE VNKVIEQARA DKKVGGSLEA AVTLYADADL AAKLNALGDE LRFVLLTSGA NVADYASASA DAQQSELLKG LKVALSKAEG EKCPRCWHYT TDIGKVAEHA EICGRCVSNV AGDGEQRKFA
NT Sequence
GTGTTTATCA AACCGAAATA CGGAACCGAG AATCTGATGA GTGACTATAA ATCGACCCTG AATTTGCCGG AAACAGGGTT CCCTATGCGT GGCGACCTCG CCAAACGCGA ACCGGGAATG CTGGCGCGTT GGACCGATGA TGACCTGTAC GGCATCATTC GTGCAGCCAA AAAAGGCAAA AAAACCTTCA TTCTGCATGA TGGCCCTCCT TATGCGAATG GCAGCATTCA TATTGGTCAC TCGGTAAACA AGATTCTGAA AGACATTATC GTTAAGTCCA AAGGACTGAC GGGCTATGAC TCGCCTTACG TTCCGGGCTG GGACTGCCAC GGTCTGCCGA TCGAGCTGAA AGTGGAGCAG GAATACGGCA AGCCGGGTGA GAAATTTACC GCTGCCGAAT TCCGCGCTAA GTGCCGCGAA TATGCCGCCG AGCAGATCGA TGGCCAGCGT AAAGACTTTA TCCGTCTCGG CGTGCTGGGC GACTGGTCGC ATCCGTACCT GACCATGGAC TTCAAAACCG AAGCCAACAT CATCCGCGCG CTGGGCAAAA TCATCGGTAA TGGCCACCTG CATAAAGGCG CCAAACCGGT GCACTGGTGC GTAGACTGCC GTTCCGCGCT GGCGGAAGCG GAAGTGGAGT ATTACGACAA AACCTCTCCG TCTATCGATG TCGCGTTCCA CGCGGTAGAC AAAGCCGCGG TACTGGCGAA ATTCGGCGTA GCCGACGTCA ACGGTCCGGT GTCGCTGGTT ATCTGGACCA CCACGCCGTG GACCCTGCCG GCCAACCGCG CTATCTCTCT GTCTCCTGAG TTCGACTATG CGCTGGTGCA GGTTGACGGC CAGGCGCTGA TCCTGGCGAA AGACCTGGTT GAGAGCGTAA TGAAGCGCGT TGGCGCGACG GACTACACTA TTCTGGCGGC GGTACAAGGT TCTGAGCTTG AGCTGATGCG CTTTAAGCAT CCGTTCCTCG ATTTCGACGT GCCGGCGATC CTTGGCGACC ACGTGACGCT GGATGCCGGT ACCGGTGCGG TGCATACCGC TGGCGGCCAC GGTCCGGACG ACTACACCAT CAGCCAGAAA TACGGTCTGG AAATCGCCAA CCCGGTTGGC CCGGACGGCG CTTACCTGCC TGGCACCTGG CCGTCCCTGG ACGGCATTAA TGTTTTCAAA GCCAATGACA TCATCGTTGA GATGCTGCGT GAGCGTGGCG CGCTGTTACA CGTTGAGAAA CTGCAGCACA GCTATCCATG CTGCTGGCGC CACAAGTCGC CGATCATCTT CCGCGCCACT CCGCAGTGGT TCGTGAGCAT GGATCAGAAA GGCCTGCGCG CGCAGTCGCT GAAAGAGATC AAAGGCGTGC AGTGGATCCC GGACTGGGGC CAGGCGCGTA TCGAATCGAT GGTCGCTAAC CGCCCTGACT GGTGTATCTC CCGTCAGCGC ACCTGGGGCG TGCCGATGTC GCTGTTCGTG CATAAAGAAA CCCATGAACT GCACCCGCGT ACCCTTGAGC TGATGGAAGA AGTGGCAAAA CGCGTTGAAG TTGACGGCAT CCAAGCGTGG TGGGATCTCG ATTCCCGCGA CATTCTTGGC GATGACGCCG ACAGCTACGA GAAAGTGCCT GATACCCTCG ACGTCTGGTT TGACTCCGGG TCGACCCACT CTTCCGTGGT TGACGTGCGT CCGGAATTCG CCGGTCACGC CGCCGATATG TATCTGGAAG GTTCTGACCA GCATCGCGGC TGGTTCATGT CCTCGCTGAT GATTTCCACG GCGATGAAAG GCAAAGCGCC ATATCGTCAG GTACTGACCC ACGGCTTCAC CGTCGACGGT CAGGGCCGCA AAATGTCCAA GTCTATCGGC AACACCGTTA GCCCGCAGGA CGTGATGAAC AAGCTCGGCG CCGATATCCT GCGCCTGTGG GTGGCTTCCA CCGACTACAC CGGCGAAATG GCGGTCTCTG ACGAGATCCT CAAACGCGCT GCGGACAGCT ATCGTCGTAT CCGTAACACC GCGCGCTTCC TGCTGGCGAA CCTTAACGGT TTCGATCCGG TGAAAGACAT GGTTAAACCG GAAGAGATGG TGGTGCTGGA CCGCTGGGCG GTCGGCTGCG CGCAGGCGGC ACAGGAAGAT ATCCTGAAAG CCTACGAATC CTACGACTTC CATGAAGTGG TGCAGCGCCT GATGCGCTTC TGCTCCATCG AGATGGGCTC GTTCTACCTC GACATCATCA AAGACCGCCA GTACACCGCC AAGGCGGACA GCGTGGCGCG TCGCAGCTGT CAGACCGCGC TGTTCCATAT CGTGGAAGCG CTGGTGCGCT GGATGGCGCC GATCATGTCC TTCACCGCAG ATGAAATCTG GGGCTATCTG CCGGGCGAGC GTGAGAAATA TGTCTTCACC GGCGAGTGGT ATGAAGGTCT GTTCGGCCTG GCCGACGACG AAGCGATGAA CGACGATTTC TGGGATGAGC TGCTGAAGGT GCGTGGCGAA GTCAACAAGG TGATCGAGCA GGCGCGTGCG GATAAGAAAG TCGGCGGCTC GCTGGAAGCG GCAGTCACGC TGTATGCTGA TGCCGATCTG GCGGCTAAGC TCAATGCGCT GGGCGATGAA TTGCGATTTG TCCTGTTGAC CTCCGGGGCG AACGTCGCAG ACTATGCGAG CGCGTCCGCT GATGCGCAGC AAAGCGAACT GCTCAAAGGG CTGAAAGTCG CATTGAGTAA AGCTGAAGGT GAGAAATGCC CGCGCTGCTG GCATTACACC ACCGATATCG GCAAGGTGGC GGAACACGCG GAAATCTGCG GACGCTGTGT GAGCAACGTC GCCGGTGACG GCGAGCAACG TAAATTCGCC
Details for KlpnC.18708.a.B1.GE44350
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 107kDa
OBSERVED MW: 107kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 30
Validated AA Sequence
MAHHHHHHMV FIKPKYGTEN LMSDYKSTLN LPETGFPMRG DLAKREPGML ARWTDDDLYG IIRAAKKGKK TFILHDGPPY ANGSIHIGHS VNKILKDIIV KSKGLTGYDS PYVPGWDCHG LPIELKVEQE YGKPGEKFTA AEFRAKCREY AAEQIDGQRK DFIRLGVLGD WSHPYLTMDF KTEANIIRAL GKIIGNGHLH KGAKPVHWCV DCRSALAEAE VEYYDKTSPS IDVAFHAVDK AAVLAKFGVX DVNGPVSLVI WTTTPWTLPA NRAISLSPEF DYALVXXTAX R
Validated NT Sequence
annnnnnann actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgg tgtttatcaa accgaaatac ggaaccgaga atctgatgag tgactataaa tcgaccctga atttgccgga aacagggttc cctatgcgtg gcgacctcgc caaacgcgaa ccgggaatgc tggcgcgttg gaccgatgat gacctgtacg gcatcattcg tgcagccaaa aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact cggtaaacaa gattctgaaa gacattatcg ttaagtccaa aggactgacg ggctatgact cgccttacgt tccgggctgg gactgccacg gtctgccgat cgagctgaaa gtggagcagg aatacggcaa gccgggtgag aaatttaccg ctgccgaatt ccgcgctaag tgccgcgaat atgccgccga gcagatcgat ggccagcgta aagactttat ccgtctcggc gtgctgggcg actggtcgca tccgtacctg accatggact tcaaaaccga agccaacatc atccgcgcgc tgggcaaaat catcggtaat ggccacctgc ataaaggcgc caaaccggtg cactggtgcg tagactgccg ttccgcgctg gcggaagcgg aagtggagta ttacgacaaa acctctccgt ctatcgatgt cgcgttccac gcggtagaca aagccgcggt actggcgaaa ttcggcgtan ccgacgtcaa cggtccggtg tcgctggtta tctggaccac cacgccgtgg accctgccgg ccaaccgcgc tatctctctg tctcctgagt tcgactatgc gctggtgcnn ntgacggcca gncgctgatc ctggcgaaag acctggtttg anagcgtaat gaagcgcgtt ggcgcgacga ctacnctatt tctggngncg gtacnnnnnn tgagcttgag ctgatgcgct ttaagcatcc gttccnncna tttcnacgtg cccggcgatc nttg
Expected Protein Sequence
MAHHHHHHMF IKPKYGTENL MSDYKSTLNL PETGFPMRGD LAKREPGMLA RWTDDDLYGI IRAAKKGKKT FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGLTGYDSP YVPGWDCHGL PIELKVEQEY GKPGEKFTAA EFRAKCREYA AEQIDGQRKD FIRLGVLGDW SHPYLTMDFK TEANIIRALG KIIGNGHLHK GAKPVHWCVD CRSALAEAEV EYYDKTSPSI DVAFHAVDKA AVLAKFGVAD VNGPVSLVIW TTTPWTLPAN RAISLSPEFD YALVQVDGQA LILAKDLVES VMKRVGATDY TILAAVQGSE LELMRFKHPF LDFDVPAILG DHVTLDAGTG AVHTAGGHGP DDYTISQKYG LEIANPVGPD GAYLPGTWPS LDGINVFKAN DIIVEMLRER GALLHVEKLQ HSYPCCWRHK SPIIFRATPQ WFVSMDQKGL RAQSLKEIKG VQWIPDWGQA RIESMVANRP DWCISRQRTW GVPMSLFVHK ETHELHPRTL ELMEEVAKRV EVDGIQAWWD LDSRDILGDD ADSYEKVPDT LDVWFDSGST HSSVVDVRPE FAGHAADMYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG RKMSKSIGNT VSPQDVMNKL GADILRLWVA STDYTGEMAV SDEILKRAAD SYRRIRNTAR FLLANLNGFD PVKDMVKPEE MVVLDRWAVG CAQAAQEDIL KAYESYDFHE VVQRLMRFCS IEMGSFYLDI IKDRQYTAKA DSVARRSCQT ALFHIVEALV RWMAPIMSFT ADEIWGYLPG EREKYVFTGE WYEGLFGLAD DEAMNDDFWD ELLKVRGEVN KVIEQARADK KVGGSLEAAV TLYADADLAA KLNALGDELR FVLLTSGANV ADYASASADA QQSELLKGLK VALSKAEGEK CPRCWHYTTD IGKVAEHAEI CGRCVSNVAG DGEQRKFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgttta tcaaaccgaa atacggaacc gagaatctga tgagtgacta taaatcgacc ctgaatttgc cggaaacagg gttccctatg cgtggcgacc tcgccaaacg cgaaccggga atgctggcgc gttggaccga tgatgacctg tacggcatca ttcgtgcagc caaaaaaggc aaaaaaacct tcattctgca tgatggccct ccttatgcga atggcagcat tcatattggt cactcggtaa acaagattct gaaagacatt atcgttaagt ccaaaggact gacgggctat gactcgcctt acgttccggg ctgggactgc cacggtctgc cgatcgagct gaaagtggag caggaatacg gcaagccggg tgagaaattt accgctgccg aattccgcgc taagtgccgc gaatatgccg ccgagcagat cgatggccag cgtaaagact ttatccgtct cggcgtgctg ggcgactggt cgcatccgta cctgaccatg gacttcaaaa ccgaagccaa catcatccgc gcgctgggca aaatcatcgg taatggccac ctgcataaag gcgccaaacc ggtgcactgg tgcgtagact gccgttccgc gctggcggaa gcggaagtgg agtattacga caaaacctct ccgtctatcg atgtcgcgtt ccacgcggta gacaaagccg cggtactggc gaaattcggc gtagccgacg tcaacggtcc ggtgtcgctg gttatctgga ccaccacgcc gtggaccctg ccggccaacc gcgctatctc tctgtctcct gagttcgact atgcgctggt gcaggttgac ggccaggcgc tgatcctggc gaaagacctg gttgagagcg taatgaagcg cgttggcgcg acggactaca ctattctggc ggcggtacaa ggttctgagc ttgagctgat gcgctttaag catccgttcc tcgatttcga cgtgccggcg atccttggcg accacgtgac gctggatgcc ggtaccggtg cggtgcatac cgctggcggc cacggtccgg acgactacac catcagccag aaatacggtc tggaaatcgc caacccggtt ggcccggacg gcgcttacct gcctggcacc tggccgtccc tggacggcat taatgttttc aaagccaatg acatcatcgt tgagatgctg cgtgagcgtg gcgcgctgtt acacgttgag aaactgcagc acagctatcc atgctgctgg cgccacaagt cgccgatcat cttccgcgcc actccgcagt ggttcgtgag catggatcag aaaggcctgc gcgcgcagtc gctgaaagag atcaaaggcg tgcagtggat cccggactgg ggccaggcgc gtatcgaatc gatggtcgct aaccgccctg actggtgtat ctcccgtcag cgcacctggg gcgtgccgat gtcgctgttc gtgcataaag aaacccatga actgcacccg cgtacccttg agctgatgga agaagtggca aaacgcgttg aagttgacgg catccaagcg tggtgggatc tcgattcccg cgacattctt ggcgatgacg ccgacagcta cgagaaagtg cctgataccc tcgacgtctg gtttgactcc gggtcgaccc actcttccgt ggttgacgtg cgtccggaat tcgccggtca cgccgccgat atgtatctgg aaggttctga ccagcatcgc ggctggttca tgtcctcgct gatgatttcc acggcgatga aaggcaaagc gccatatcgt caggtactga cccacggctt caccgtcgac ggtcagggcc gcaaaatgtc caagtctatc ggcaacaccg ttagcccgca ggacgtgatg aacaagctcg gcgccgatat cctgcgcctg tgggtggctt ccaccgacta caccggcgaa atggcggtct ctgacgagat cctcaaacgc gctgcggaca gctatcgtcg tatccgtaac accgcgcgct tcctgctggc gaaccttaac ggtttcgatc cggtgaaaga catggttaaa ccggaagaga tggtggtgct ggaccgctgg gcggtcggct gcgcgcaggc ggcacaggaa gatatcctga aagcctacga atcctacgac ttccatgaag tggtgcagcg cctgatgcgc ttctgctcca tcgagatggg ctcgttctac ctcgacatca tcaaagaccg ccagtacacc gccaaggcgg acagcgtggc gcgtcgcagc tgtcagaccg cgctgttcca tatcgtggaa gcgctggtgc gctggatggc gccgatcatg tccttcaccg cagatgaaat ctggggctat ctgccgggcg agcgtgagaa atatgtcttc accggcgagt ggtatgaagg tctgttcggc ctggccgacg acgaagcgat gaacgacgat ttctgggatg agctgctgaa ggtgcgtggc gaagtcaaca aggtgatcga gcaggcgcgt gcggataaga aagtcggcgg ctcgctggaa gcggcagtca cgctgtatgc tgatgccgat ctggcggcta agctcaatgc gctgggcgat gaattgcgat ttgtcctgtt gacctccggg gcgaacgtcg cagactatgc gagcgcgtcc gctgatgcgc agcaaagcga actgctcaaa gggctgaaag tcgcattgag taaagctgaa ggtgagaaat gcccgcgctg ctggcattac accaccgata tcggcaaggt ggcggaacac gcggaaatct gcggacgctg tgtgagcaac gtcgccggtg acggcgagca acgtaaattc gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for KlpnC.18708.a.A3.PW39336
PURIFICATION DATe: 11/24/2024
CONCENTRATION: 11.7mg/ml
OBSERVED MW: 107kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDYKSTLN LPETGFPMRG DLAKREPGML ARWTDDDLYG IIRAAKKGKK TFILHDGPPY ANGSIHIGHS VNKILKDIIV KSKGLTGYDS PYVPGWDCHG LPIELKVEQE YGKPGEKFTA AEFRAKCREY AAEQIDGQRK DFIRLGVLGD WSHPYLTMDF KTEANIIRAL GKIIGNGHLH KGAKPVHWCV DCRSALAEAE VEYYDKTSPS IDVAFHAVDK AAVLAKFGVA DVNGPVSLVI WTTTPWTLPA NRAISLSPEF DYALVQVDGQ ALILAKDLVE SVMKRVGATD YTILAAVQGS ELELMRFKHP FLDFDVPAIL GDHVTLDAGT GAVHTAGGHG PDDYTISQKY GLEIANPVGP DGAYLPGTWP SLDGINVFKA NDIIVEMLRE RGALLHVEKL QHSYPCCWRH KSPIIFRATP QWFVSMDQKG LRAQSLKEIK GVQWIPDWGQ ARIESMVANR PDWCISRQRT WGVPMSLFVH KETHELHPRT LELMEEVAKR VEVDGIQAWW DLDSRDILGD DADSYEKVPD TLDVWFDSGS THSSVVDVRP EFAGHAADMY LEGSDQHRGW FMSSLMISTA MKGKAPYRQV LTHGFTVDGQ GRKMSKSIGN TVSPQDVMNK LGADILRLWV ASTDYTGEMA VSDEILKRAA DSYRRIRNTA RFLLANLNGF DPVKDMVKPE EMVVLDRWAV GCAQAAQEDI LKAYESYDFH EVVQRLMRFC SIEMGSFYLD IIKDRQYTAK ADSVARRSCQ TALFHIVEAL VRWMAPIMSF TADEIWGYLP GEREKYVFTG EWYEGLFGLA DDEAMNDDFW DELLKVRGEV NKVIEQARAD KKVGGSLEAA VTLYADADLA AKLNALGDEL RFVLLTSGAN VADYASASAD AQQSELLKGL KVALSKAEGE KCPRCWHYTT DIGKVAEHAE ICGRCVSNVA GDGEQRKFA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGACTAT AAATCGACCC TGAATTTGCC GGAAACAGGG TTCCCTATGC GTGGCGACCT CGCCAAACGC GAACCGGGAA TGCTGGCGCG TTGGACCGAT GATGACCTGT ACGGCATCAT TCGTGCAGCC AAAAAAGGCA AAAAAACCTT CATTCTGCAT GATGGCCCTC CTTATGCGAA TGGCAGCATT CATATTGGTC ACTCGGTAAA CAAGATTCTG AAAGACATTA TCGTTAAGTC CAAAGGACTG ACGGGCTATG ACTCGCCTTA CGTTCCGGGC TGGGACTGCC ACGGTCTGCC GATCGAGCTG AAAGTGGAGC AGGAATACGG CAAGCCGGGT GAGAAATTTA CCGCTGCCGA ATTCCGCGCT AAGTGCCGCG AATATGCCGC CGAGCAGATC GATGGCCAGC GTAAAGACTT TATCCGTCTC GGCGTGCTGG GCGACTGGTC GCATCCGTAC CTGACCATGG ACTTCAAAAC CGAAGCCAAC ATCATCCGCG CGCTGGGCAA AATCATCGGT AATGGCCACC TGCATAAAGG CGCCAAACCG GTGCACTGGT GCGTAGACTG CCGTTCCGCG CTGGCGGAAG CGGAAGTGGA GTATTACGAC AAAACCTCTC CGTCTATCGA TGTCGCGTTC CACGCGGTAG ACAAAGCCGC GGTACTGGCG AAATTCGGCG TAGCCGACGT CAACGGTCCG GTGTCGCTGG TTATCTGGAC CACCACGCCG TGGACCCTGC CGGCCAACCG CGCTATCTCT CTGTCTCCTG AGTTCGACTA TGCGCTGGTG CAGGTTGACG GCCAGGCGCT GATCCTGGCG AAAGACCTGG TTGAGAGCGT AATGAAGCGC GTTGGCGCGA CGGACTACAC TATTCTGGCG GCGGTACAAG GTTCTGAGCT TGAGCTGATG CGCTTTAAGC ATCCGTTCCT CGATTTCGAC GTGCCGGCGA TCCTTGGCGA CCACGTGACG CTGGATGCCG GTACCGGTGC GGTGCATACC GCTGGCGGCC ACGGTCCGGA CGACTACACC ATCAGCCAGA AATACGGTCT GGAAATCGCC AACCCGGTTG GCCCGGACGG CGCTTACCTG CCTGGCACCT GGCCGTCCCT GGACGGCATT AATGTTTTCA AAGCCAATGA CATCATCGTT GAGATGCTGC GTGAGCGTGG CGCGCTGTTA CACGTTGAGA AACTGCAGCA CAGCTATCCA TGCTGCTGGC GCCACAAGTC GCCGATCATC TTCCGCGCCA CTCCGCAGTG GTTCGTGAGC ATGGATCAGA AAGGCCTGCG CGCGCAGTCG CTGAAAGAGA TCAAAGGCGT GCAGTGGATC CCGGACTGGG GCCAGGCGCG TATCGAATCG ATGGTCGCTA ACCGCCCTGA CTGGTGTATC TCCCGTCAGC GCACCTGGGG CGTGCCGATG TCGCTGTTCG TGCATAAAGA AACCCATGAA CTGCACCCGC GTACCCTTGA GCTGATGGAA GAAGTGGCAA AACGCGTTGA AGTTGACGGC ATCCAAGCGT GGTGGGATCT CGATTCCCGC GACATTCTTG GCGATGACGC CGACAGCTAC GAGAAAGTGC CTGATACCCT CGACGTCTGG TTTGACTCCG GGTCGACCCA CTCTTCCGTG GTTGACGTGC GTCCGGAATT CGCCGGTCAC GCCGCCGATA TGTATCTGGA AGGTTCTGAC CAGCATCGCG GCTGGTTCAT GTCCTCGCTG ATGATTTCCA CGGCGATGAA AGGCAAAGCG CCATATCGTC AGGTACTGAC CCACGGCTTC ACCGTCGACG GTCAGGGCCG CAAAATGTCC AAGTCTATCG GCAACACCGT TAGCCCGCAG GACGTGATGA ACAAGCTCGG CGCCGATATC CTGCGCCTGT GGGTGGCTTC CACCGACTAC ACCGGCGAAA TGGCGGTCTC TGACGAGATC CTCAAACGCG CTGCGGACAG CTATCGTCGT ATCCGTAACA CCGCGCGCTT CCTGCTGGCG AACCTTAACG GTTTCGATCC GGTGAAAGAC ATGGTTAAAC CGGAAGAGAT GGTGGTGCTG GACCGCTGGG CGGTCGGCTG CGCGCAGGCG GCACAGGAAG ATATCCTGAA AGCCTACGAA TCCTACGACT TCCATGAAGT GGTGCAGCGC CTGATGCGCT TCTGCTCCAT CGAGATGGGC TCGTTCTACC TCGACATCAT CAAAGACCGC CAGTACACCG CCAAGGCGGA CAGCGTGGCG CGTCGCAGCT GTCAGACCGC GCTGTTCCAT ATCGTGGAAG CGCTGGTGCG CTGGATGGCG CCGATCATGT CCTTCACCGC AGATGAAATC TGGGGCTATC TGCCGGGCGA GCGTGAGAAA TATGTCTTCA CCGGCGAGTG GTATGAAGGT CTGTTCGGCC TGGCCGACGA CGAAGCGATG AACGACGATT TCTGGGATGA GCTGCTGAAG GTGCGTGGCG AAGTCAACAA GGTGATCGAG CAGGCGCGTG CGGATAAGAA AGTCGGCGGC TCGCTGGAAG CGGCAGTCAC GCTGTATGCT GATGCCGATC TGGCGGCTAA GCTCAATGCG CTGGGCGATG AATTGCGATT TGTCCTGTTG ACCTCCGGGG CGAACGTCGC AGACTATGCG AGCGCGTCCG CTGATGCGCA GCAAAGCGAA CTGCTCAAAG GGCTGAAAGT CGCATTGAGT AAAGCTGAAG GTGAGAAATG CCCGCGCTGC TGGCATTACA CCACCGATAT CGGCAAGGTG GCGGAACACG CGGAAATCTG CGGACGCTGT GTGAGCAACG TCGCCGGTGA CGGCGAGCAA CGTAAATTCG CCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA