KlpnC.18582.a

Protein-methionine-sulfoxide reductase catalytic subunit MsrP

CENTER ID: KlpnC.18582.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18582.a.B2.GE44349 Mature protein(KlpnC.18582.a) 48 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4681
UniProt: A0A0H3GZ50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRKKLTEAD VTAESVFMLK RRQMLKMLGI SATALSLPAA AQADLLDWFK GHDRPPAPAG KALEFAKPAE WQANLTLTPE DKVAGYNNFY EFGLDKADPA ANAGSLRTDP WTLTIGGEVA KPLTLDHDDL TKRFPLEERI YRMRCVEAWS MVVPWVGFPL HKLLALVEPT SSARYVAFKT LYAPDQMPGQ KDRFIGGGLA YPYVEGLRLD EAMHPLTLLT VGVYGKALPP QNGAPVRLTV PWKYGFKGIK SIVSIELTRE RPPTTWNLAA PDEYGFFANV NPHVDHPRWS QASERFIGAG GVLDVKRQPT LLFNGYADEV ASLYRGMNLR ENF
NT Sequence
ATGAAAAGAA AAAAACTGAC GGAAGCCGAC GTCACGGCCG AATCCGTTTT TATGCTCAAG CGGCGCCAGA TGCTGAAAAT GCTGGGCATC AGCGCGACCG CGCTTTCCCT TCCCGCCGCC GCGCAGGCCG ACCTGCTGGA CTGGTTTAAA GGCCACGACC GCCCACCAGC GCCAGCGGGC AAGGCCCTGG AGTTTGCTAA ACCCGCCGAG TGGCAGGCTA ATTTGACGCT TACCCCGGAA GATAAGGTCG CCGGTTACAA TAATTTTTAT GAATTCGGCC TCGATAAAGC CGATCCGGCG GCGAACGCGG GTAGCCTGCG CACCGATCCC TGGACGCTCA CCATCGGTGG CGAAGTGGCG AAACCTCTGA CCCTTGACCA TGACGATCTG ACCAAACGCT TCCCGCTGGA AGAGCGCATT TATCGTATGC GCTGTGTGGA AGCCTGGTCG ATGGTGGTCC CCTGGGTCGG CTTCCCGCTG CATAAACTGC TGGCGCTGGT GGAGCCCACC AGCAGCGCGC GCTATGTGGC CTTCAAAACC CTCTATGCTC CGGATCAAAT GCCCGGCCAG AAAGACCGGT TTATCGGCGG GGGGCTCGCT TACCCTTACG TGGAGGGGCT ACGGCTGGAT GAAGCGATGC ACCCGCTGAC GCTACTCACC GTCGGGGTGT ATGGCAAAGC GCTGCCGCCG CAAAACGGCG CGCCGGTCCG CCTGACCGTG CCGTGGAAGT ACGGTTTTAA AGGCATAAAA TCCATTGTTA GTATCGAGCT GACCCGCGAG CGGCCGCCAA CCACCTGGAA CCTGGCGGCG CCGGACGAAT ACGGCTTCTT CGCCAACGTC AATCCGCACG TCGATCACCC CCGCTGGTCG CAGGCTAGCG AGCGGTTTAT CGGCGCCGGC GGCGTGCTCG ATGTGAAGCG TCAACCGACG TTGCTGTTTA ATGGCTATGC CGACGAAGTG GCGTCGCTGT ATCGCGGCAT GAATCTGCGG GAGAACTTC
Details for KlpnC.18582.a.B2.GE44349
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMW FKGHDRPPAP AGKALEFAKP AEWQANLTLT PEDKVAGYNN FYEFGLDKAD PAANAGSLRT DPWTLTIGGE VAKPLTLDHD DLTKRFPLEE RIYRMRCVEA WSMVVPWVGF PLHKLLALVE PTSSARYVAF KTLYAPDQMP GQKDRFIGGG LAYPYVEGLR LDEAMHPLTL LTVGVYGKAL PPQNGAPVRL TVPWKYGFKG IKSIVSIELT RERPPTTWNL AAPDEYGFFA NVNPHVDHPR WSHXRAVYRR RRRARCEASP TLLFNGYARR SGVAVSRHES XXXTSX
Validated NT Sequence
tacgactcnc tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atgtggttta aaggccacga ccgcccacca gcgccagcgg gcaaggccct ggagtttgct aaacccgccg agtggcaggc taatttgacg cttaccccgg aagataaggt cgccggttac aataattttt atgaattcgg cctcgataaa gccgatccgg cggcgaacgc gggtagcctg cgcaccgatc cctggacgct caccatcggt ggcgaagtgg cgaaacctct gacccttgac catgacgatc tgaccaaacg cttcccgctg gaagagcgca tttatcgtat gcgctgtgtg gaagcctggt cgatggtggt cccctgggtc ggcttcccgc tgcataaact gctggcgctg gtggagccca ccagcagcgc gcgctatgtg gccttcaaaa ccctctatgc tccggatcaa atgcccggcc agaaagaccg gtttatcggc ggggggctcg cttaccctta cgtggagggg ctacggctgg atgaagcgat gcacccgctg acgctactca ccgtcggggt gtatggcaaa gcgctgccgc cgcaaaacgg cgcgccggtc cgcctgaccg tgccgtggaa gtacggtttt aaaggcataa aatccattgt tagtatcgag ctgacccgcg agcggccgcc aaccacctgg aacctggcgg cgccggacga atacggcttc ttcgccaacg tcaatccgca cgtcgatcac ccccgctggt cgcactancg agcggtttat cggcgccggc ggcgtgctcg atgtgaagcg tcaccgacgt tgctgtttaa tggctatgcc cgacgaagtg gcgtcgctgt atcgcggcat gaatcctgnn gganaacttc nnagtgagta agatangatc ccggctgcta cancccgaaa nnagctgagt tggctgctgc cnccgctgag catactanca taaccccttg gggcctnnta aanggtcttg gaggg
Expected Protein Sequence
MAHHHHHHWF KGHDRPPAPA GKALEFAKPA EWQANLTLTP EDKVAGYNNF YEFGLDKADP AANAGSLRTD PWTLTIGGEV AKPLTLDHDD LTKRFPLEER IYRMRCVEAW SMVVPWVGFP LHKLLALVEP TSSARYVAFK TLYAPDQMPG QKDRFIGGGL AYPYVEGLRL DEAMHPLTLL TVGVYGKALP PQNGAPVRLT VPWKYGFKGI KSIVSIELTR ERPPTTWNLA APDEYGFFAN VNPHVDHPRW SQASERFIGA GGVLDVKRQP TLLFNGYADE VASLYRGMNL RENF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattggttta aaggccacga ccgcccacca gcgccagcgg gcaaggccct ggagtttgct aaacccgccg agtggcaggc taatttgacg cttaccccgg aagataaggt cgccggttac aataattttt atgaattcgg cctcgataaa gccgatccgg cggcgaacgc gggtagcctg cgcaccgatc cctggacgct caccatcggt ggcgaagtgg cgaaacctct gacccttgac catgacgatc tgaccaaacg cttcccgctg gaagagcgca tttatcgtat gcgctgtgtg gaagcctggt cgatggtggt cccctgggtc ggcttcccgc tgcataaact gctggcgctg gtggagccca ccagcagcgc gcgctatgtg gccttcaaaa ccctctatgc tccggatcaa atgcccggcc agaaagaccg gtttatcggc ggggggctcg cttaccctta cgtggagggg ctacggctgg atgaagcgat gcacccgctg acgctactca ccgtcggggt gtatggcaaa gcgctgccgc cgcaaaacgg cgcgccggtc cgcctgaccg tgccgtggaa gtacggtttt aaaggcataa aatccattgt tagtatcgag ctgacccgcg agcggccgcc aaccacctgg aacctggcgg cgccggacga atacggcttc ttcgccaacg tcaatccgca cgtcgatcac ccccgctggt cgcaggctag cgagcggttt atcggcgccg gcggcgtgct cgatgtgaag cgtcaaccga cgttgctgtt taatggctat gccgacgaag tggcgtcgct gtatcgcggc atgaatctgc gggagaactt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat