KlpnC.18461.b

2-octaprenyl-6-methoxyphenyl hydroxylase

CENTER ID: KlpnC.18461.b
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18461.b.B2.GE44347 Mature protein(KlpnC.18461.b) 18 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4307
UniProt: A0A0H3GY55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVLIVGGGM TGATLALAIS RLTGGALPVH LIEAQDPHSS RHPGFDDRAI ALAAGTCQQL ARIGIWQRLA ERATPIQRVH VSDRGHAGFV NLAAADYGLS ALGQVVELHD VGQRLFGLLR EAPGVTLHCP AKVEAVSRSQ ESVSLTLEGG EIINGKLLVA ADGSRSALGA RCGISWQQQP YEQIAIIANV STALPHEGRA FERFTEHGPL AMLPMSQGRC SLVWCHPQSR RDEVQSWSDE RFCQELQQAF GWRLGRITHA GKRSVYPLAL TTASRAVSHR LALVGNAAQT LHPIAGQGFN LGLRDVMSLA ELLADAHLSG EDVGHYPLLC RYQARRAGDK AATIGVTDGL VHLFANRWAP LVAGRNVGLM AMELFTPARD ALAQRTLGWV PR
NT Sequence
ATGAGCGTGC TGATCGTCGG GGGCGGGATG ACCGGCGCGA CGCTCGCGCT GGCCATCTCC CGTTTGACTG GCGGCGCGCT GCCGGTCCAC CTCATTGAAG CGCAGGATCC GCATTCTTCA CGCCACCCTG GCTTTGACGA CCGGGCGATC GCCCTGGCTG CGGGCACCTG CCAGCAGCTG GCGCGTATCG GTATCTGGCA ACGCCTCGCC GAACGCGCGA CGCCCATCCA GCGAGTACAC GTCAGCGACC GCGGTCATGC CGGGTTTGTG AATCTGGCGG CCGCGGATTA TGGCCTGTCG GCGCTGGGCC AGGTGGTTGA ACTCCACGAT GTCGGCCAGC GGCTGTTTGG TCTGCTGCGC GAGGCGCCGG GCGTCACGCT GCACTGTCCG GCGAAAGTGG AAGCGGTCAG TCGCAGCCAG GAGAGCGTCA GCCTTACGCT TGAGGGCGGC GAAATCATTA ACGGTAAGCT GCTGGTGGCG GCGGACGGTT CCCGTTCGGC ACTGGGCGCC CGCTGCGGCA TCAGCTGGCA GCAGCAGCCG TATGAGCAGA TAGCGATCAT TGCTAACGTC AGCACCGCGT TGCCCCACGA AGGTCGCGCC TTCGAGCGCT TTACCGAGCA TGGCCCGCTG GCGATGCTGC CGATGTCGCA AGGGCGCTGC TCGCTGGTGT GGTGTCATCC GCAGTCGCGC CGCGATGAGG TGCAGAGCTG GTCTGACGAA CGCTTTTGTC AGGAGCTGCA GCAGGCGTTC GGCTGGCGGC TGGGGCGTAT TACCCACGCC GGGAAACGCA GCGTCTATCC GCTGGCCCTC ACCACCGCCA GCCGCGCGGT GTCGCACCGC CTGGCGCTGG TCGGCAATGC GGCGCAGACG CTGCACCCGA TCGCCGGGCA GGGCTTTAAT CTCGGCCTGC GCGATGTGAT GAGCCTGGCG GAATTACTCG CTGACGCCCA TCTCAGCGGC GAAGATGTCG GTCATTATCC GCTGCTGTGC CGCTATCAGG CGCGCAGGGC AGGGGATAAA GCCGCCACCA TCGGCGTGAC CGATGGGCTG GTGCATCTGT TTGCCAACCG TTGGGCGCCG CTGGTGGCGG GGCGCAACGT CGGTCTGATG GCGATGGAAT TATTTACCCC GGCGCGCGAT GCGCTGGCGC AGCGTACCCT CGGTTGGGTT CCCCGT
Details for KlpnC.18461.b.B2.GE44347
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAI SRLTGGALPV HLIEAQDPHS SRHPGFDDRA IALAAGTCQQ LARIGIWQRL AERATPIQRV HVSDRGHAGF VNLAAADYGL SALGQVVELH DVGQRLFGLL REAPGVTLHC PAKVEAVSRS QESVSLTLEG GEIINGKLLV AADGSRSALG ARCGISWQQQ PYEQIAIIAN VSTALPHEGR AFERFTEHGP LAMLPMSQGR CSLVWCHPQS RRDEVQSWSD ERFCQELQQA FGWRLGRITH AGKRSVYPLA LTTASRAVSH RLALVGNAAQ TLHPIAGQGF NLGLRDVMSL AELLADAHLS GEDVGHYPLL CRYQARRAGD KAATIGVTDG LVHLFANRWA PLVAGRNVGL MAMELFTPAR DALAQRTLGW VPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccatct cccgtttgac tggcggcgcg ctgccggtcc acctcattga agcgcaggat ccgcattctt cacgccaccc tggctttgac gaccgggcga tcgccctggc tgcgggcacc tgccagcagc tggcgcgtat cggtatctgg caacgcctcg ccgaacgcgc gacgcccatc cagcgagtac acgtcagcga ccgcggtcat gccgggtttg tgaatctggc ggccgcggat tatggcctgt cggcgctggg ccaggtggtt gaactccacg atgtcggcca gcggctgttt ggtctgctgc gcgaggcgcc gggcgtcacg ctgcactgtc cggcgaaagt ggaagcggtc agtcgcagcc aggagagcgt cagccttacg cttgagggcg gcgaaatcat taacggtaag ctgctggtgg cggcggacgg ttcccgttcg gcactgggcg cccgctgcgg catcagctgg cagcagcagc cgtatgagca gatagcgatc attgctaacg tcagcaccgc gttgccccac gaaggtcgcg ccttcgagcg ctttaccgag catggcccgc tggcgatgct gccgatgtcg caagggcgct gctcgctggt gtggtgtcat ccgcagtcgc gccgcgatga ggtgcagagc tggtctgacg aacgcttttg tcaggagctg cagcaggcgt tcggctggcg gctggggcgt attacccacg ccgggaaacg cagcgtctat ccgctggccc tcaccaccgc cagccgcgcg gtgtcgcacc gcctggcgct ggtcggcaat gcggcgcaga cgctgcaccc gatcgccggg cagggcttta atctcggcct gcgcgatgtg atgagcctgg cggaattact cgctgacgcc catctcagcg gcgaagatgt cggtcattat ccgctgctgt gccgctatca ggcgcgcagg gcaggggata aagccgccac catcggcgtg accgatgggc tggtgcatct gtttgccaac cgttgggcgc cgctggtggc ggggcgcaac gtcggtctga tggcgatgga attatttacc ccggcgcgcg atgcgctggc gcagcgtacc ctcggttggg ttccccgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat