KlpnC.18461.a

2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

CENTER ID: KlpnC.18461.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18461.a.B1.GE44346 Full length(KlpnC.18461.a) 1 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|1125630.4.peg.1487
UniProt: A0A0H3GK01

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIHVTDVAI VGGGMVGGAL ALGLAQQGFT VTVLEKAAPP AFDPASAPDV RISAISAASV GLLKSLGVWD AIRAMRVHAY RRLETWEWES AHVAFDAAEL KLPELGYMVE NKVLQWGLWQ ALAAHEAVTL CVGSELKTMQ RGETQTALHL REGETIHARL VIGADGANSQ VREMAGIGVH AWQYQQSCML ISVECADDPG DSTWQQFTPS GPRAFLPLFD HWASLVWYDA PARIRQLQSM TMAQLQQEIA SHFPARLGRV TPQAAGAFPL TRRHALQYVQ PGLALVGDAA HTIHPLAGQG VNLGYRDVDA LLEILAEARG RGEDWASLPV LKRYQARRRA DNFIMQSGMD LFYAGFSNDL APVRMLRNIG LMAAERAGVL KRQALKYALG L
NT Sequence
ATGACAATTC ACGTAACAGA TGTCGCCATC GTGGGCGGCG GCATGGTCGG CGGCGCCCTG GCGTTAGGGC TGGCGCAACA GGGATTTACC GTGACGGTGC TTGAGAAAGC GGCGCCACCG GCGTTCGATC CGGCATCCGC CCCGGATGTG CGGATCTCGG CGATCAGCGC GGCTTCGGTC GGCCTGCTGA AGAGTCTCGG CGTCTGGGAT GCCATACGGG CGATGCGCGT CCACGCGTAT CGCCGTCTGG AGACCTGGGA GTGGGAGAGC GCGCACGTCG CCTTCGACGC CGCGGAGCTC AAGCTGCCGG AGCTGGGCTA TATGGTCGAG AACAAGGTTC TGCAATGGGG CCTGTGGCAA GCGCTGGCGG CGCATGAGGC GGTTACCCTG TGCGTCGGTA GCGAACTGAA AACGATGCAG CGCGGCGAGA CGCAGACGGC GTTGCATTTG CGCGAGGGGG AGACGATCCA CGCCCGGCTG GTTATCGGCG CCGACGGCGC TAACTCCCAG GTGCGGGAGA TGGCCGGCAT CGGCGTGCAC GCCTGGCAAT ATCAGCAGTC CTGTATGCTG ATCAGCGTCG AATGCGCCGA CGATCCCGGC GACAGCACCT GGCAGCAGTT TACTCCCAGC GGCCCACGCG CCTTCCTGCC GCTGTTCGAC CACTGGGCTT CGCTGGTCTG GTACGACGCC CCGGCGCGGA TCCGTCAGCT GCAGAGCATG ACCATGGCGC AGCTCCAGCA GGAGATCGCC AGTCACTTTC CGGCGCGGCT GGGACGGGTG ACGCCGCAGG CGGCCGGCGC CTTCCCGCTC ACCCGGCGTC ATGCGCTGCA GTATGTTCAG CCCGGCCTGG CGCTGGTGGG CGATGCGGCG CACACCATTC ATCCACTGGC GGGGCAGGGG GTTAACCTTG GCTATCGTGA TGTCGATGCC CTGCTGGAGA TTCTGGCGGA AGCGCGCGGC CGCGGCGAAG ACTGGGCCAG CCTGCCGGTG CTGAAGCGCT ACCAGGCGCG GCGGCGAGCG GATAATTTTA TCATGCAGTC GGGGATGGAT CTGTTTTATG CCGGTTTCAG CAATGACCTG GCGCCGGTGC GGATGCTGCG TAATATTGGC CTGATGGCGG CTGAACGCGC CGGGGTGCTG AAGCGTCAGG CGCTGAAGTA TGCCTTAGGC TTG
Details for KlpnC.18461.a.B1.GE44346
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMT IHVTDVAIVG GGMVGGALAL GLAQQGFTVT VLEKAAPPAF DPASAPDVRI SAISAASVGL LKSLGVWDAI RAMRVHAYRR LETWEWESAH VAFDAAELKL PELGYMVENK VLQWGLWQAL AAHEAVTLRV GSELKTMQRG ETQTALHLRE GETIHARLVI GADGANSQVR EMAGIGVHAW QYQQSCMLIS VECADDPGDS TWQQFTPSGP RAFLPLFDHW ASLVWYDAPA RIRQLQSMTM AQLQQEIASH FPARLGRVTP QAAGAFPLTR RHALQYVQPG LALVGDAAHT IHPLAGQGVN LGYRDVDALL EILAEVRGRG EDWASLPVLK RYQARRRADN FIMQSGMDLF YAGFSNDLAP VRMLRNIGLM AAERAGVLKR QAL
Validated NT Sequence
cttcagcgcc tgacgcttca gcaccccggc gcgttcagcc gccatcaggc caatattacg cagcatccgc accggcgcca ggtcattgct gaaaccggca taaaagaggt ccatccccga ctgcatgata aaattatccg ctcgccgccg cgcctggtag cgcttcagca ccggcaggct ggcccagtct tcgccgcggc cgcgcacttc cgccaggatc tccagcaggg catcgacatc acgatagcca aggttaaccc cctgccccgc cagtggatga atggtgtgcg ccgcatcgcc caccagcgcc aggccgggct gaacatactg cagcgcatga cgccgggtga gcgggaaggc gccggccgcc tgcggcgtca cccgtcccag ccgcgccgga aagtgactgg cgatctcctg ctggagctgc gccatggtca tgctctgcag ctgacggatc cgcgccgggg cgtcgtacca gaccagcgag gcccagtggt cgaacagcgg caggaaggcg cgcgggccgc tgggagtaaa ctgctgccag gtgctgtcgc cgggatcgtc ggcgcattcg acgctgatca gcatacagga ctgctgatat tgccaggcgt gcacgccgat gccggccatc tcccgcacct gggagttagc gccgtcggcg ccgataacca gccgggcgtg gatcgtctcc ccctcgcgca aatgcaacgc cgtctgcgtc tcgccgcgct gcatcgtttt cagttcgcta ccgacgcgca gggtaaccgc ctcatgcgcc gccagcgctt gccacaggcc ccattgcaga accttgttct cgaccatata gcccagctcc ggcagcttga gctccgcggc gtcgaaggcg acgtgcgcgc tctcccactc ccaggtctcc agacggcgat aagcatggac gcgcatcgcc cgtatggcat cccagacgcc gagactcttc agcaggccga ccgaagccgc gctgatcgcc gagatccgca catccggggc ggatgccgga tcgaacgccg gtggcgccgc tttctcaagc accgtcacgg taaatccctg ttgcgccagc cctaacgcca gggcgccgcc gaccatgccg ccgcccacga tggcgacatc tgttacgtga attgtcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMT IHVTDVAIVG GGMVGGALAL GLAQQGFTVT VLEKAAPPAF DPASAPDVRI SAISAASVGL LKSLGVWDAI RAMRVHAYRR LETWEWESAH VAFDAAELKL PELGYMVENK VLQWGLWQAL AAHEAVTLCV GSELKTMQRG ETQTALHLRE GETIHARLVI GADGANSQVR EMAGIGVHAW QYQQSCMLIS VECADDPGDS TWQQFTPSGP RAFLPLFDHW ASLVWYDAPA RIRQLQSMTM AQLQQEIASH FPARLGRVTP QAAGAFPLTR RHALQYVQPG LALVGDAAHT IHPLAGQGVN LGYRDVDALL EILAEARGRG EDWASLPVLK RYQARRRADN FIMQSGMDLF YAGFSNDLAP VRMLRNIGLM AAERAGVLKR QALKYALGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa ttcacgtaac agatgtcgcc atcgtgggcg gcggcatggt cggcggcgcc ctggcgttag ggctggcgca acagggattt accgtgacgg tgcttgagaa agcggcgcca ccggcgttcg atccggcatc cgccccggat gtgcggatct cggcgatcag cgcggcttcg gtcggcctgc tgaagagtct cggcgtctgg gatgccatac gggcgatgcg cgtccacgcg tatcgccgtc tggagacctg ggagtgggag agcgcgcacg tcgccttcga cgccgcggag ctcaagctgc cggagctggg ctatatggtc gagaacaagg ttctgcaatg gggcctgtgg caagcgctgg cggcgcatga ggcggttacc ctgtgcgtcg gtagcgaact gaaaacgatg cagcgcggcg agacgcagac ggcgttgcat ttgcgcgagg gggagacgat ccacgcccgg ctggttatcg gcgccgacgg cgctaactcc caggtgcggg agatggccgg catcggcgtg cacgcctggc aatatcagca gtcctgtatg ctgatcagcg tcgaatgcgc cgacgatccc ggcgacagca cctggcagca gtttactccc agcggcccac gcgccttcct gccgctgttc gaccactggg cttcgctggt ctggtacgac gccccggcgc ggatccgtca gctgcagagc atgaccatgg cgcagctcca gcaggagatc gccagtcact ttccggcgcg gctgggacgg gtgacgccgc aggcggccgg cgccttcccg ctcacccggc gtcatgcgct gcagtatgtt cagcccggcc tggcgctggt gggcgatgcg gcgcacacca ttcatccact ggcggggcag ggggttaacc ttggctatcg tgatgtcgat gccctgctgg agattctggc ggaagcgcgc ggccgcggcg aagactgggc cagcctgccg gtgctgaagc gctaccaggc gcggcggcga gcggataatt ttatcatgca gtcggggatg gatctgtttt atgccggttt cagcaatgac ctggcgccgg tgcggatgct gcgtaatatt ggcctgatgg cggctgaacg cgccggggtg ctgaagcgtc aggcgctgaa gtatgcctta ggcttgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t