KlpnC.18288.a
3-phosphoshikimate 1-carboxyvinyltransferase
| CENTER ID: | KlpnC.18288.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.18288.a.B1.GE44341 | Full length(KlpnC.18288.a) | 1 | 427 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7TM4
DEPOSITED: 1/19/2022
DETERMINATION: XRay
CLONE: KlpnC.18288.a.B1.GE44341
PROTEIN: KlpnC.18288.a.B1.PW39049
7TM5
DEPOSITED: 1/19/2022
DETERMINATION: XRay
CLONE: KlpnC.18288.a.B1.GE44341
PROTEIN: KlpnC.18288.a.B1.PW39049
7TM6
DEPOSITED: 1/19/2022
DETERMINATION: XRay
CLONE: KlpnC.18288.a.B1.GE44341
PROTEIN: KlpnC.18288.a.B1.PW39049
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.1768 |
| UniProt: | A0A0H3GV01 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESLTLQPIA RVEGTVNLPG SKSVSNRALL LAALARGTTV LTNLLDSDDV RHMLNALSAL GVQYTLSADR TRCEVTGNGG PLRSAAALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRQGGAQI DYLEQENYPP LRLRGGFQGG NVEVDGSVSS QFLTALLMTA PLAPQDTVIV IKGDLVSKPY IDITLHLMKT FGVEVDNQSY QRFVVRGKQQ YQSPGDYLVE GDASSASYFL AAGAIKGGTV KVTGIGRNSV QGDIRFADVL EKMGATVTWG DDFIACTHGE LKAVDMDMNH IPDAAMTIAT AALFAQGTTT LRNIYNWRVK ETDRLFAMAT ELRKVGAEVE EGEDYIRITP PAKLKYAEIG TYNDHRMAMC FSLVALSDTP VTILDPKCTA KTFPDYFEQL ARISTLA
NT Sequence
ATGGAATCCC TGACGTTACA ACCCATCGCA CGCGTAGAGG GCACCGTGAA CCTGCCAGGT TCGAAAAGCG TCTCCAACCG CGCGCTGCTG CTGGCTGCCC TGGCCCGCGG CACGACGGTG CTGACCAACC TGCTGGACAG CGACGATGTG CGCCATATGC TGAATGCCCT GAGTGCGCTG GGGGTTCAAT ATACCCTGTC TGCCGACCGC ACCCGCTGCG AAGTGACCGG CAACGGCGGC CCGTTGCGCT CTGCCGCGGC GCTGGAGCTG TTCCTTGGCA ACGCCGGTAC CGCGATGCGT CCGCTGGCGG CGGCTCTGTG CCTTGGCAGC AACGATATCG TGCTGACCGG CGAACCGCGG ATGAAAGAGC GTCCGATCGG CCATCTGGTG GATGCCCTGC GTCAGGGCGG CGCTCAGATC GACTATCTGG AGCAGGAAAA TTATCCACCG CTGCGCCTGC GCGGCGGTTT TCAGGGCGGC AACGTCGAGG TTGACGGCAG CGTCTCCAGC CAGTTCCTGA CCGCGCTGCT GATGACCGCG CCGCTGGCGC CGCAGGATAC CGTCATCGTC ATTAAGGGCG ATCTGGTGTC GAAACCCTAT ATCGACATTA CGCTGCACCT AATGAAAACC TTCGGCGTTG AGGTGGACAA TCAGTCTTAT CAGCGATTTG TGGTGCGCGG CAAGCAGCAG TATCAGTCGC CGGGAGATTA CCTGGTGGAA GGCGATGCTT CCTCGGCCTC GTATTTCCTC GCCGCTGGCG CCATTAAGGG CGGCACGGTA AAAGTCACCG GTATCGGCCG CAACAGCGTA CAGGGCGACA TTCGTTTCGC CGACGTACTG GAGAAAATGG GCGCCACCGT CACCTGGGGC GACGATTTCA TCGCCTGCAC CCACGGCGAG CTCAAGGCGG TCGATATGGA TATGAACCAT ATCCCGGACG CCGCGATGAC CATCGCCACC GCCGCGCTGT TCGCGCAAGG GACCACTACC CTGCGCAATA TCTACAACTG GCGGGTCAAG GAGACGGACC GTCTGTTCGC CATGGCGACC GAGCTGCGTA AAGTGGGCGC CGAGGTGGAA GAGGGGGAAG ATTATATCCG CATCACCCCG CCGGCAAAGT TGAAGTACGC AGAAATCGGC ACCTATAACG ACCACCGGAT GGCGATGTGC TTCTCGCTGG TAGCGCTGTC TGATACGCCG GTGACCATCC TTGATCCCAA ATGTACGGCC AAAACGTTCC CTGACTACTT TGAGCAGCTG GCGCGGATCA GTACCCTCGC C
Details for KlpnC.18288.a.B1.GE44341
| HARVESTED ON: | 5/21/2021 |
| SEQUENCED ON: | 6/4/2021 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 50 |
Validated AA Sequence
MAHHHHHHME SLTLQPIARV EGTVNLPGSK SVSNRALLLA ALARGTTVLT NLLDSDDVRH MLNALSALGV QYTLSADRTR CEVTGNGGPL RSAAALELFL GNAGTAMRPL AAALCLGSND IVLTGEPRMK ERPIGHLVDA LRQGGAQIDC LEQENYPPLR LRGGFQGGNV EVDGSVSSQF LTALLMTAPL APQDTVIVIK GDLVSKPYID ITLHLMKNLX R
Validated NT Sequence
anntcnctat agggagacca caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg gaatccctga cgttacaacc catcgcacgc gtagagggca ccgtgaacct gccaggttcg aaaagcgtct ccaaccgcgc gctgctgctg gctgccctgg cccgcggcac gacggtgctg accaacctgc tggacagcga cgatgtgcgc catatgctga atgccctgag tgcgctgggg gttcaatata ccctgtctgc cgaccgcacc cgctgcgaag tgaccggcaa cggcggcccg ttgcgctctg ccgcggcgct ggagctgttc cttggcaacg ccggtaccgc gatgcgtccg ctggcggcgg ctctgtgcct tggcagcaac gatatcgtgc tgaccggcga accgcggatg aaagagcgtc cgatcggcca tctggtggat gccctgcgtc agggcggcgc tcagatcgac tgtctggagc aggaaaatta tccaccgctg cgcctgcgcg gcggttttca gggcggcaac gtcgaggttg acggcagcgt ctccagccag ttcctgaccg cgctgctgat gaccgcgccg ctggcgccgc aggataccgt catcgtcatt aagggcgatc tggtgtcgaa accctatatc gacattacgc tgcacctgat gaaaaacctt cngcgttgan gtggacnatc antcttatca gcgatttgtg gtgcgcggca aagcagcant atcagtcgcc gggagattac ctggtggaag gcgatgcttc ctcggcctcg tatttcctcn ccgctgggcg ccattaaggg cggcacggta aaagtcnccg ggtatcggcc gcaacancg
Expected Protein Sequence
MAHHHHHHME SLTLQPIARV EGTVNLPGSK SVSNRALLLA ALARGTTVLT NLLDSDDVRH MLNALSALGV QYTLSADRTR CEVTGNGGPL RSAAALELFL GNAGTAMRPL AAALCLGSND IVLTGEPRMK ERPIGHLVDA LRQGGAQIDY LEQENYPPLR LRGGFQGGNV EVDGSVSSQF LTALLMTAPL APQDTVIVIK GDLVSKPYID ITLHLMKTFG VEVDNQSYQR FVVRGKQQYQ SPGDYLVEGD ASSASYFLAA GAIKGGTVKV TGIGRNSVQG DIRFADVLEK MGATVTWGDD FIACTHGELK AVDMDMNHIP DAAMTIATAA LFAQGTTTLR NIYNWRVKET DRLFAMATEL RKVGAEVEEG EDYIRITPPA KLKYAEIGTY NDHRMAMCFS LVALSDTPVT ILDPKCTAKT FPDYFEQLAR ISTLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat ccctgacgtt acaacccatc gcacgcgtag agggcaccgt gaacctgcca ggttcgaaaa gcgtctccaa ccgcgcgctg ctgctggctg ccctggcccg cggcacgacg gtgctgacca acctgctgga cagcgacgat gtgcgccata tgctgaatgc cctgagtgcg ctgggggttc aatataccct gtctgccgac cgcacccgct gcgaagtgac cggcaacggc ggcccgttgc gctctgccgc ggcgctggag ctgttccttg gcaacgccgg taccgcgatg cgtccgctgg cggcggctct gtgccttggc agcaacgata tcgtgctgac cggcgaaccg cggatgaaag agcgtccgat cggccatctg gtggatgccc tgcgtcaggg cggcgctcag atcgactatc tggagcagga aaattatcca ccgctgcgcc tgcgcggcgg ttttcagggc ggcaacgtcg aggttgacgg cagcgtctcc agccagttcc tgaccgcgct gctgatgacc gcgccgctgg cgccgcagga taccgtcatc gtcattaagg gcgatctggt gtcgaaaccc tatatcgaca ttacgctgca cctaatgaaa accttcggcg ttgaggtgga caatcagtct tatcagcgat ttgtggtgcg cggcaagcag cagtatcagt cgccgggaga ttacctggtg gaaggcgatg cttcctcggc ctcgtatttc ctcgccgctg gcgccattaa gggcggcacg gtaaaagtca ccggtatcgg ccgcaacagc gtacagggcg acattcgttt cgccgacgta ctggagaaaa tgggcgccac cgtcacctgg ggcgacgatt tcatcgcctg cacccacggc gagctcaagg cggtcgatat ggatatgaac catatcccgg acgccgcgat gaccatcgcc accgccgcgc tgttcgcgca agggaccact accctgcgca atatctacaa ctggcgggtc aaggagacgg accgtctgtt cgccatggcg accgagctgc gtaaagtggg cgccgaggtg gaagaggggg aagattatat ccgcatcacc ccgccggcaa agttgaagta cgcagaaatc ggcacctata acgaccaccg gatggcgatg tgcttctcgc tggtagcgct gtctgatacg ccggtgacca tccttgatcc caaatgtacg gccaaaacgt tccctgacta ctttgagcag ctggcgcgga tcagtaccct cgcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat