KlpnC.18181.a

Histidinol dehydrogenase

CENTER ID: KlpnC.18181.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18181.a.B1.GE44340 Full length(KlpnC.18181.a) 1 434
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3446
UniProt: A0A0H3GQM4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFNTIIDWN GCSADQQQQL LTRPAISASD SISKTVTEIL NNVKANGDAA LREYSAKFDK TTVAALQVSE AEIAAAGERL SDELKQAMAV AVKNIETFHN AQQLQAVDVE TLPGVRCQQV TRPIASVGLY IPGGSAPLFS TVLMLATPAR IAGCQQVVLC SPPPIADEIL YAAQLCGVKT IFNVGGAQAI AALALGTESV PKVDKIFGPG NAYVTEAKRQ VSQRLDGAAI DMPAGPSEVL VIADSGANPD FVASDLLSQA EHGPDSQVIL LTPDADMGSR VAEAVERQLA ALPRAETARV ALSASRIIVA RDLAQCVAIS NLYGPEHLII QTRQARELVD SITSAGSVFL GDWSPESAGD YASGTNHVLP TYGYTATCSS LGLADFQKRM TVQELSRDGF AALASTIEIL AAAERLDAHK NAVTLRVAAL KEQA
NT Sequence
ATGAGCTTCA ACACAATCAT CGACTGGAAC GGCTGTAGCG CAGACCAACA ACAGCAACTC TTAACGCGCC CGGCGATTTC CGCCTCCGAC AGCATCAGTA AAACGGTGAC GGAGATCCTG AATAACGTCA AAGCCAACGG CGACGCGGCG CTGCGCGAAT ACAGCGCGAA GTTTGATAAA ACCACCGTCG CCGCGCTGCA GGTGAGCGAG GCGGAGATCG CCGCCGCCGG CGAGCGCCTC AGCGATGAGC TGAAGCAGGC GATGGCGGTG GCAGTGAAAA ACATCGAGAC CTTCCACAAC GCGCAACAGC TGCAGGCGGT GGATGTCGAA ACGCTGCCGG GGGTGCGCTG CCAGCAGGTC ACCCGCCCTA TCGCCTCCGT CGGACTGTAC ATTCCCGGCG GCTCAGCGCC GCTGTTCTCC ACGGTGCTGA TGCTGGCGAC GCCGGCGCGC ATCGCCGGCT GTCAGCAGGT GGTGCTCTGC TCGCCACCGC CGATTGCCGA TGAGATCCTC TATGCCGCGC AGCTGTGCGG CGTGAAAACC ATCTTTAACG TCGGCGGCGC GCAGGCCATC GCCGCCCTGG CCCTCGGTAC CGAGTCGGTG CCGAAGGTGG ATAAAATCTT TGGCCCGGGT AACGCTTACG TCACGGAAGC CAAACGCCAG GTCAGCCAGC GTCTGGACGG CGCAGCCATC GACATGCCGG CCGGCCCGTC GGAAGTGCTG GTCATCGCCG ACAGCGGCGC CAACCCGGAC TTTGTCGCCT CTGACCTGCT GTCGCAGGCG GAACACGGCC CGGACTCGCA GGTGATCCTC CTGACCCCGG ACGCCGACAT GGGCAGCCGC GTCGCCGAAG CCGTGGAGCG CCAGCTGGCC GCCCTGCCGC GCGCGGAAAC CGCCCGCGTG GCGCTCTCCG CCAGCCGGAT CATCGTGGCC CGCGATCTGG CGCAGTGCGT GGCTATCTCC AACCTGTACG GCCCGGAGCA CCTGATCATT CAGACCCGTC AGGCCCGCGA GCTGGTGGAC AGCATCACCA GCGCCGGTTC CGTTTTTCTT GGCGACTGGT CACCGGAATC CGCGGGCGAC TACGCCTCGG GCACCAACCA CGTCCTGCCG ACCTATGGTT ACACCGCCAC CTGCTCAAGC CTCGGCCTGG CCGACTTCCA GAAGCGGATG ACCGTGCAGG AGCTGTCGCG CGACGGCTTT GCCGCCCTCG CCTCAACGAT TGAGATCCTG GCCGCCGCCG AGCGCCTTGA CGCCCACAAA AACGCCGTAA CGCTGCGCGT TGCCGCCCTG AAGGAGCAAG CA
Details for KlpnC.18181.a.B1.GE44340
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 82
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMS FNTIIDWNGC SADQQQQLLT RPAISASDSI SKTVTEILNN VKANGDAALR EYSAKFDKTT VAALQVSEAE IAAAGERLSD ELKQAMAVAV KNIETFHNAQ QLQAVDVETL PGVRCQQVTR PIASVGLYIP GGSAPLFSTV LMLATPARIA GCQQVVLCSP PPIADEILYA AQLCGVKTIF NVGGAQAXAA LXLGTESVPK VDKIFGPGNA YVTEAKRQVX QRLDGAAXXM PAGPXEVLVI ADSGANPDFV ASXLLSXRXX GRTXXDPLTR TPTXGSRVAX PXXASWPPFX APKLPXXXSX XXDHRXRDXX QXXXXXXXXG XEXLDXSNPS A
Validated NT Sequence
gngagtccta taggngagan gnacgncggt ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atgagcttca acacaatcat cgactggaac ggctgtagcg cagaccaaca acagcaactc ttaacgcgcc cggcgatttc cgcctccgac agcatcagta aaacggtgac ggagatcctg aataacgtca aagccaacgg cgacgcggcg ctgcgcgaat acagcgcgaa gtttgataaa accaccgtcg ccgcgctgca ggtgagcgag gcggagatcg ccgccgccgg cgagcgcctc agcgatgagc tgaagcaggc gatggcggtg gcagtgaaaa acatcgagac cttccacaac gcgcaacagc tgcaggcggt ggatgtcgaa acgctgccgg gggtgcgctg ccagcaggtc acccgtccta tcgcctccgt cggactgtac attcccggcg gctcagcgcc gctgttctcc acggtgctga tgctggcgac gccggcgcgc atcgccggct gtcagcaggt ggtgctctgc tcgccgccgc cgattgccga tgagatcctc tatgccgcgc agctgtgcgg cgtaaaaacc atctttaacg tcggcggcgc ccaggccntc gccgccctcg ncctcggtac cgagtcggtg ccgaaggtgg ataaaatctt tggcccgggc aacgcttacg tcacggaagc caaacgccag gtcanccagc gtctggacgg cgcagctatn nacatgccgg ccggcccgnc ngaagtgctg gtcatcgccg acagcggcgc caacccggac tttgtcgcct ccnacctgct gtcgcnncgg aanncgggcc ggactcncag ngatcctctg acccggacgc cgacntnggg cagccgcgtc gccnanccnn gnanngccag ctggccgccc ttcnnngcgc cgaaactgcc cncgnggnnc tctcnncnnn cggatcatcg tnnccgcgat ntggngcagt gngngnnnan ntncnccngn ancggccngg agnacttgga tcnttcnaac ccgtcngccc c
Expected Protein Sequence
MAHHHHHHMS FNTIIDWNGC SADQQQQLLT RPAISASDSI SKTVTEILNN VKANGDAALR EYSAKFDKTT VAALQVSEAE IAAAGERLSD ELKQAMAVAV KNIETFHNAQ QLQAVDVETL PGVRCQQVTR PIASVGLYIP GGSAPLFSTV LMLATPARIA GCQQVVLCSP PPIADEILYA AQLCGVKTIF NVGGAQAIAA LALGTESVPK VDKIFGPGNA YVTEAKRQVS QRLDGAAIDM PAGPSEVLVI ADSGANPDFV ASDLLSQAEH GPDSQVILLT PDADMGSRVA EAVERQLAAL PRAETARVAL SASRIIVARD LAQCVAISNL YGPEHLIIQT RQARELVDSI TSAGSVFLGD WSPESAGDYA SGTNHVLPTY GYTATCSSLG LADFQKRMTV QELSRDGFAA LASTIEILAA AERLDAHKNA VTLRVAALKE QA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct tcaacacaat catcgactgg aacggctgta gcgcagacca acaacagcaa ctcttaacgc gcccggcgat ttccgcctcc gacagcatca gtaaaacggt gacggagatc ctgaataacg tcaaagccaa cggcgacgcg gcgctgcgcg aatacagcgc gaagtttgat aaaaccaccg tcgccgcgct gcaggtgagc gaggcggaga tcgccgccgc cggcgagcgc ctcagcgatg agctgaagca ggcgatggcg gtggcagtga aaaacatcga gaccttccac aacgcgcaac agctgcaggc ggtggatgtc gaaacgctgc cgggggtgcg ctgccagcag gtcacccgcc ctatcgcctc cgtcggactg tacattcccg gcggctcagc gccgctgttc tccacggtgc tgatgctggc gacgccggcg cgcatcgccg gctgtcagca ggtggtgctc tgctcgccac cgccgattgc cgatgagatc ctctatgccg cgcagctgtg cggcgtgaaa accatcttta acgtcggcgg cgcgcaggcc atcgccgccc tggccctcgg taccgagtcg gtgccgaagg tggataaaat ctttggcccg ggtaacgctt acgtcacgga agccaaacgc caggtcagcc agcgtctgga cggcgcagcc atcgacatgc cggccggccc gtcggaagtg ctggtcatcg ccgacagcgg cgccaacccg gactttgtcg cctctgacct gctgtcgcag gcggaacacg gcccggactc gcaggtgatc ctcctgaccc cggacgccga catgggcagc cgcgtcgccg aagccgtgga gcgccagctg gccgccctgc cgcgcgcgga aaccgcccgc gtggcgctct ccgccagccg gatcatcgtg gcccgcgatc tggcgcagtg cgtggctatc tccaacctgt acggcccgga gcacctgatc attcagaccc gtcaggcccg cgagctggtg gacagcatca ccagcgccgg ttccgttttt cttggcgact ggtcaccgga atccgcgggc gactacgcct cgggcaccaa ccacgtcctg ccgacctatg gttacaccgc cacctgctca agcctcggcc tggccgactt ccagaagcgg atgaccgtgc aggagctgtc gcgcgacggc tttgccgccc tcgcctcaac gattgagatc ctggccgccg ccgagcgcct tgacgcccac aaaaacgccg taacgctgcg cgttgccgcc ctgaaggagc aagcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat