KlpnC.18113.a

Acetolactate synthase

CENTER ID: KlpnC.18113.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18113.a.B1.GE44339 Full length(KlpnC.18113.a) 1 548
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18113.a.B1.PW39053 Full length(KlpnC.18113.a) 1 548

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.113
UniProt: A0A0H3GLI6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNGAQWVVHA LRTQGVDTVF GYPGGAIMPV YDALYDGGVE HLLCRHEQGA AMAAIGYARA TGKTGVCIAT SGPGATNLIT GLADALLDSI PIVAITGQVA APFIGTDAFQ EVDVLGLSLA CTKHSFLVQS LDELPRVIAE AFQVANSGRP GPVLVDIPKD IQMAQGDLDP HFSTVADEMA FPQAEVAQAL QMLAQSQQPM LYVGGGVGMA QAVPALREFL AVTRMPATCT LKGLGVVDAD YPYYLGMLGM HGTKAANLAV QECDLLIAVG ARFDDRVTGK LNTFAPHAKV IHMDIDPAEL NKLRQAHIGL TGDLNCLLPA LQQPLAIDGW RERSAALRAE HAWRYDHPGE AIYAPLLLKQ LSDRKPADSV VTTDVGQHQM WSAQHMTYTR PENFITSSGL GTMGFGLPAA VGAQVARPND TVICISGDGS FMMNVQELGT VKRKQLPLKI VLLDNQRLGM VRQWQQLFFQ ERYSETTLTD NPDFLTLASA FGIPGQHITR KDQVEAALDT MLSSQGPYLL HVSIDELENV WPLVPPGASN SEMLEKLS
NT Sequence
ATGAATGGCG CGCAGTGGGT GGTACATGCT TTGCGGACAC AGGGAGTCGA CACAGTTTTT GGCTATCCGG GTGGCGCAAT TATGCCGGTT TACGATGCAC TGTATGACGG CGGCGTGGAG CACCTGCTGT GCCGGCATGA GCAAGGGGCA GCCATGGCGG CCATTGGTTA TGCGCGAGCA ACCGGTAAAA CCGGGGTGTG TATCGCGACG TCCGGCCCGG GCGCGACCAA TCTGATCACC GGCCTGGCGG ACGCCCTGCT TGACTCTATT CCCATCGTAG CTATCACCGG CCAGGTGGCC GCACCCTTCA TCGGCACGGA TGCGTTCCAG GAAGTAGATG TTCTCGGTTT GTCACTGGCC TGTACTAAGC ACAGTTTCCT TGTGCAGTCT CTGGACGAAC TGCCGCGGGT TATTGCCGAG GCTTTCCAGG TGGCAAACTC AGGCCGTCCA GGTCCGGTGC TGGTTGATAT CCCTAAGGAT ATTCAGATGG CGCAGGGCGA CCTCGATCCC CATTTCTCTA CCGTCGCCGA TGAGATGGCA TTTCCGCAGG CGGAAGTGGC GCAGGCTCTG CAGATGCTGG CCCAATCGCA GCAACCGATG CTGTATGTTG GCGGCGGGGT CGGCATGGCG CAGGCGGTAC CGGCTTTGCG CGAATTCCTG GCGGTGACCA GGATGCCGGC AACCTGCACC CTGAAAGGGC TGGGCGTGGT GGATGCTGAT TATCCCTATT ATCTCGGCAT GCTGGGAATG CACGGCACTA AGGCGGCCAA CCTGGCGGTA CAGGAGTGCG ATCTGCTGAT CGCCGTCGGC GCGCGCTTTG ACGATCGCGT AACCGGTAAG CTCAATACCT TCGCACCGCA TGCCAAAGTG ATCCACATGG ACATCGATCC GGCGGAGCTG AACAAGCTGC GTCAGGCGCA TATCGGTCTG ACAGGCGATC TGAACTGCCT GCTGCCGGCG CTGCAGCAGC CGTTAGCTAT CGATGGCTGG CGCGAGCGGA GTGCCGCACT GCGTGCCGAG CATGCCTGGC GTTACGATCA TCCCGGTGAG GCAATCTACG CGCCGCTGCT GCTGAAGCAG CTTTCCGACC GCAAGCCGGC GGACAGCGTG GTGACCACCG ACGTTGGCCA GCACCAGATG TGGTCGGCTC AGCATATGAC CTATACCCGC CCGGAAAACT TTATCACCTC CAGTGGCTTA GGCACCATGG GCTTTGGCCT GCCGGCCGCC GTTGGCGCCC AGGTGGCGCG CCCTAACGAT ACGGTGATCT GTATCTCCGG TGACGGCTCC TTCATGATGA ATGTACAGGA GCTGGGCACC GTTAAACGCA AGCAGTTACC GCTGAAGATC GTGTTACTCG ACAACCAGCG GTTAGGGATG GTTCGACAAT GGCAGCAGCT GTTTTTCCAG GAACGTTATA GCGAAACCAC TCTGACTGAT AACCCTGATT TCCTCACGCT GGCCAGCGCC TTCGGTATTC CTGGCCAACA CATCACCCGT AAAGACCAGG TTGAAGCGGC ACTCGACACC ATGCTTTCGA GCCAGGGGCC ATACCTGCTT CATGTCTCAA TCGACGAACT TGAGAATGTC TGGCCGTTGG TGCCGCCCGG CGCCAGTAAC TCTGAAATGC TGGAGAAATT ATCA
Details for KlpnC.18113.a.B1.GE44339
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 87
PERCENT COVERAGE: 57
Validated AA Sequence
MAHHHHHHMN GAQWVVHALR TQGVDTVFGY PGGAIMPVYD ALYDGGVEHL LCRHEQGAAM AAIGYARATG KTGVCIATSG PGATNLITGL ADALLDSIPI VAITGQVAAP FIGTDAFQEV DVLGLSLACT KHSFLVQSLD ELPRVIAEAF QVANSGRPGP VLVDIPKDIQ MAQGDLDPHF STVADEMAFP QAEVAXALQM LAQSQQPMLY VGGGVGMAXA VPALREFLAV XRMPATCXLK GLGVVDADYP YYLGMLGMXG TKXXXLAVQE CDLLIAVXAR FXRSRNXXAS IPSXPHXXSD PXGTSIRRXL NXLRXXHXXV
Validated NT Sequence
tnnnncnact cnntntaggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg gcgcgcagtg ggtggtacat gctttgcgga cacagggagt cgacacagtt tttggctatc cgggtggcgc aattatgccg gtttacgatg cactgtatga cggcggcgtg gagcacctgc tgtgccggca tgagcaaggg gcagccatgg cggccattgg ttatgcgcga gcaaccggta aaaccggggt gtgtatcgcg acgtccggcc cgggcgcgac caatctgatc accggcctgg cggacgccct gcttgactct attcccatcg tagctatcac cggccaggtg gccgcaccct tcatcggcac ggatgcgttc caggaagtag acgttctcgg tttgtcactg gcctgtacta agcacagttt ccttgtgcag tctctggacg aactgccgcg ggttattgcc gaggctttcc aggtggcaaa ctcaggccgt ccaggtccgg tgctggttga tatccctaag gatattcaga tggcgcaggg cgacctcgat ccccatttct ctaccgtcgc cgatgagatg gcatttccgc aggcggaagt ggcgcangct ctgcagatgc tggcccaatc gcagcaaccg atgctgtatg ttggcggcgg ggtcggcatg gcgcangcgg taccggcttt gcgcgaattc ctggcggtga ncaggatgcc ggcaacctgc nccctgaaag ggctgggcgt ggtggatgct gattatccct attatctcgg catgctgggg atgcnnggca ctaaggnggn cnacctggcg gtacaggagt gcgatctgct gatcgccgtc ngcgcgcgct ttnnacgatc gcgtaaccng nnagcntcaa taccttccnn nccgcatngc nnaagtgatc cnnatggaac atcgatccgg cggganctga acnagctgcg tcnngnncat atnngggtct ganaggcnnt ctgnactgcc tgnnnncnng nnctnncagc agccggtnan ctnntnnaat gnnntgnngc cnnnancggn nnnnnnc
Expected Protein Sequence
MAHHHHHHMN GAQWVVHALR TQGVDTVFGY PGGAIMPVYD ALYDGGVEHL LCRHEQGAAM AAIGYARATG KTGVCIATSG PGATNLITGL ADALLDSIPI VAITGQVAAP FIGTDAFQEV DVLGLSLACT KHSFLVQSLD ELPRVIAEAF QVANSGRPGP VLVDIPKDIQ MAQGDLDPHF STVADEMAFP QAEVAQALQM LAQSQQPMLY VGGGVGMAQA VPALREFLAV TRMPATCTLK GLGVVDADYP YYLGMLGMHG TKAANLAVQE CDLLIAVGAR FDDRVTGKLN TFAPHAKVIH MDIDPAELNK LRQAHIGLTG DLNCLLPALQ QPLAIDGWRE RSAALRAEHA WRYDHPGEAI YAPLLLKQLS DRKPADSVVT TDVGQHQMWS AQHMTYTRPE NFITSSGLGT MGFGLPAAVG AQVARPNDTV ICISGDGSFM MNVQELGTVK RKQLPLKIVL LDNQRLGMVR QWQQLFFQER YSETTLTDNP DFLTLASAFG IPGQHITRKD QVEAALDTML SSQGPYLLHV SIDELENVWP LVPPGASNSE MLEKLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg gcgcgcagtg ggtggtacat gctttgcgga cacagggagt cgacacagtt tttggctatc cgggtggcgc aattatgccg gtttacgatg cactgtatga cggcggcgtg gagcacctgc tgtgccggca tgagcaaggg gcagccatgg cggccattgg ttatgcgcga gcaaccggta aaaccggggt gtgtatcgcg acgtccggcc cgggcgcgac caatctgatc accggcctgg cggacgccct gcttgactct attcccatcg tagctatcac cggccaggtg gccgcaccct tcatcggcac ggatgcgttc caggaagtag atgttctcgg tttgtcactg gcctgtacta agcacagttt ccttgtgcag tctctggacg aactgccgcg ggttattgcc gaggctttcc aggtggcaaa ctcaggccgt ccaggtccgg tgctggttga tatccctaag gatattcaga tggcgcaggg cgacctcgat ccccatttct ctaccgtcgc cgatgagatg gcatttccgc aggcggaagt ggcgcaggct ctgcagatgc tggcccaatc gcagcaaccg atgctgtatg ttggcggcgg ggtcggcatg gcgcaggcgg taccggcttt gcgcgaattc ctggcggtga ccaggatgcc ggcaacctgc accctgaaag ggctgggcgt ggtggatgct gattatccct attatctcgg catgctggga atgcacggca ctaaggcggc caacctggcg gtacaggagt gcgatctgct gatcgccgtc ggcgcgcgct ttgacgatcg cgtaaccggt aagctcaata ccttcgcacc gcatgccaaa gtgatccaca tggacatcga tccggcggag ctgaacaagc tgcgtcaggc gcatatcggt ctgacaggcg atctgaactg cctgctgccg gcgctgcagc agccgttagc tatcgatggc tggcgcgagc ggagtgccgc actgcgtgcc gagcatgcct ggcgttacga tcatcccggt gaggcaatct acgcgccgct gctgctgaag cagctttccg accgcaagcc ggcggacagc gtggtgacca ccgacgttgg ccagcaccag atgtggtcgg ctcagcatat gacctatacc cgcccggaaa actttatcac ctccagtggc ttaggcacca tgggctttgg cctgccggcc gccgttggcg cccaggtggc gcgccctaac gatacggtga tctgtatctc cggtgacggc tccttcatga tgaatgtaca ggagctgggc accgttaaac gcaagcagtt accgctgaag atcgtgttac tcgacaacca gcggttaggg atggttcgac aatggcagca gctgtttttc caggaacgtt atagcgaaac cactctgact gataaccctg atttcctcac gctggccagc gccttcggta ttcctggcca acacatcacc cgtaaagacc aggttgaagc ggcactcgac accatgcttt cgagccaggg gccatacctg cttcatgtct caatcgacga acttgagaat gtctggccgt tggtgccgcc cggcgccagt aactctgaaa tgctggagaa attatcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for KlpnC.18113.a.B1.PW39053
PURIFICATION DATe: 7/17/2021
CONCENTRATION: 37mg/ml
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 87
PERCENT COVERAGE: 57
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN GAQWVVHALR TQGVDTVFGY PGGAIMPVYD ALYDGGVEHL LCRHEQGAAM AAIGYARATG KTGVCIATSG PGATNLITGL ADALLDSIPI VAITGQVAAP FIGTDAFQEV DVLGLSLACT KHSFLVQSLD ELPRVIAEAF QVANSGRPGP VLVDIPKDIQ MAQGDLDPHF STVADEMAFP QAEVAXALQM LAQSQQPMLY VGGGVGMAXA VPALREFLAV XRMPATCXLK GLGVVDADYP YYLGMLGMXG TKXXXLAVQE CDLLIAVXAR FXRSRNXXAS IPSXPHXXSD PXGTSIRRXL NXLRXXHXXV
Validated NT Sequence
tnnnncnact cnntntaggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg gcgcgcagtg ggtggtacat gctttgcgga cacagggagt cgacacagtt tttggctatc cgggtggcgc aattatgccg gtttacgatg cactgtatga cggcggcgtg gagcacctgc tgtgccggca tgagcaaggg gcagccatgg cggccattgg ttatgcgcga gcaaccggta aaaccggggt gtgtatcgcg acgtccggcc cgggcgcgac caatctgatc accggcctgg cggacgccct gcttgactct attcccatcg tagctatcac cggccaggtg gccgcaccct tcatcggcac ggatgcgttc caggaagtag acgttctcgg tttgtcactg gcctgtacta agcacagttt ccttgtgcag tctctggacg aactgccgcg ggttattgcc gaggctttcc aggtggcaaa ctcaggccgt ccaggtccgg tgctggttga tatccctaag gatattcaga tggcgcaggg cgacctcgat ccccatttct ctaccgtcgc cgatgagatg gcatttccgc aggcggaagt ggcgcangct ctgcagatgc tggcccaatc gcagcaaccg atgctgtatg ttggcggcgg ggtcggcatg gcgcangcgg taccggcttt gcgcgaattc ctggcggtga ncaggatgcc ggcaacctgc nccctgaaag ggctgggcgt ggtggatgct gattatccct attatctcgg catgctgggg atgcnnggca ctaaggnggn cnacctggcg gtacaggagt gcgatctgct gatcgccgtc ngcgcgcgct ttnnacgatc gcgtaaccng nnagcntcaa taccttccnn nccgcatngc nnaagtgatc cnnatggaac atcgatccgg cggganctga acnagctgcg tcnngnncat atnngggtct ganaggcnnt ctgnactgcc tgnnnncnng nnctnncagc agccggtnan ctnntnnaat gnnntgnngc cnnnancggn nnnnnnc
Expressed Protein Sequence
MAHHHHHHMN GAQWVVHALR TQGVDTVFGY PGGAIMPVYD ALYDGGVEHL LCRHEQGAAM AAIGYARATG KTGVCIATSG PGATNLITGL ADALLDSIPI VAITGQVAAP FIGTDAFQEV DVLGLSLACT KHSFLVQSLD ELPRVIAEAF QVANSGRPGP VLVDIPKDIQ MAQGDLDPHF STVADEMAFP QAEVAQALQM LAQSQQPMLY VGGGVGMAQA VPALREFLAV TRMPATCTLK GLGVVDADYP YYLGMLGMHG TKAANLAVQE CDLLIAVGAR FDDRVTGKLN TFAPHAKVIH MDIDPAELNK LRQAHIGLTG DLNCLLPALQ QPLAIDGWRE RSAALRAEHA WRYDHPGEAI YAPLLLKQLS DRKPADSVVT TDVGQHQMWS AQHMTYTRPE NFITSSGLGT MGFGLPAAVG AQVARPNDTV ICISGDGSFM MNVQELGTVK RKQLPLKIVL LDNQRLGMVR QWQQLFFQER YSETTLTDNP DFLTLASAFG IPGQHITRKD QVEAALDTML SSQGPYLLHV SIDELENVWP LVPPGASNSE MLEKLS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG GCGCGCAGTG GGTGGTACAT GCTTTGCGGA CACAGGGAGT CGACACAGTT TTTGGCTATC CGGGTGGCGC AATTATGCCG GTTTACGATG CACTGTATGA CGGCGGCGTG GAGCACCTGC TGTGCCGGCA TGAGCAAGGG GCAGCCATGG CGGCCATTGG TTATGCGCGA GCAACCGGTA AAACCGGGGT GTGTATCGCG ACGTCCGGCC CGGGCGCGAC CAATCTGATC ACCGGCCTGG CGGACGCCCT GCTTGACTCT ATTCCCATCG TAGCTATCAC CGGCCAGGTG GCCGCACCCT TCATCGGCAC GGATGCGTTC CAGGAAGTAG ATGTTCTCGG TTTGTCACTG GCCTGTACTA AGCACAGTTT CCTTGTGCAG TCTCTGGACG AACTGCCGCG GGTTATTGCC GAGGCTTTCC AGGTGGCAAA CTCAGGCCGT CCAGGTCCGG TGCTGGTTGA TATCCCTAAG GATATTCAGA TGGCGCAGGG CGACCTCGAT CCCCATTTCT CTACCGTCGC CGATGAGATG GCATTTCCGC AGGCGGAAGT GGCGCAGGCT CTGCAGATGC TGGCCCAATC GCAGCAACCG ATGCTGTATG TTGGCGGCGG GGTCGGCATG GCGCAGGCGG TACCGGCTTT GCGCGAATTC CTGGCGGTGA CCAGGATGCC GGCAACCTGC ACCCTGAAAG GGCTGGGCGT GGTGGATGCT GATTATCCCT ATTATCTCGG CATGCTGGGA ATGCACGGCA CTAAGGCGGC CAACCTGGCG GTACAGGAGT GCGATCTGCT GATCGCCGTC GGCGCGCGCT TTGACGATCG CGTAACCGGT AAGCTCAATA CCTTCGCACC GCATGCCAAA GTGATCCACA TGGACATCGA TCCGGCGGAG CTGAACAAGC TGCGTCAGGC GCATATCGGT CTGACAGGCG ATCTGAACTG CCTGCTGCCG GCGCTGCAGC AGCCGTTAGC TATCGATGGC TGGCGCGAGC GGAGTGCCGC ACTGCGTGCC GAGCATGCCT GGCGTTACGA TCATCCCGGT GAGGCAATCT ACGCGCCGCT GCTGCTGAAG CAGCTTTCCG ACCGCAAGCC GGCGGACAGC GTGGTGACCA CCGACGTTGG CCAGCACCAG ATGTGGTCGG CTCAGCATAT GACCTATACC CGCCCGGAAA ACTTTATCAC CTCCAGTGGC TTAGGCACCA TGGGCTTTGG CCTGCCGGCC GCCGTTGGCG CCCAGGTGGC GCGCCCTAAC GATACGGTGA TCTGTATCTC CGGTGACGGC TCCTTCATGA TGAATGTACA GGAGCTGGGC ACCGTTAAAC GCAAGCAGTT ACCGCTGAAG ATCGTGTTAC TCGACAACCA GCGGTTAGGG ATGGTTCGAC AATGGCAGCA GCTGTTTTTC CAGGAACGTT ATAGCGAAAC CACTCTGACT GATAACCCTG ATTTCCTCAC GCTGGCCAGC GCCTTCGGTA TTCCTGGCCA ACACATCACC CGTAAAGACC AGGTTGAAGC GGCACTCGAC ACCATGCTTT CGAGCCAGGG GCCATACCTG CTTCATGTCT CAATCGACGA ACTTGAGAAT GTCTGGCCGT TGGTGCCGCC CGGCGCCAGT AACTCTGAAA TGCTGGAGAA ATTATCATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT