SSGCID: Target Page

KlpnC.18101.a

Aminopeptidase N

CENTER ID:	KlpnC.18101.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.18101.a.B1.GE44338	Full length(KlpnC.18101.a)		1	871

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.1799
UniProt:	A0A0H3GR26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQQPQAKYR HDYRAPEYLI SDIDLTFDLD AAKTVVTAES KVSRHAAASD VPLRLDGEDL TLIALQVNGQ PWSDYKEENN QLVIGGLPEH FTLTIVNEIS PAANTALEGL YQSGEALCTQ CEAEGFRHIT WYLDRPDVLA RFTTKIIADK AKYPFLLSNG NRMAEGELEN GRHWVQWQDP FPKPCYLFAL VAGDFDVLRD SFRTRSGREV ALELYVDRGN LDRAPWAMTS LKNSMKWDEE RFGLEYDLDI YMIVAVDFFN MGAMENKGLN VFNSKYVLAR TDTATDKDYL DIERVIGHEY FHNWTGNRVT CRDWFQLSLK EGLTVFRDQE FSSDLGSRAV NRINNVRTMR GLQFAEDASP MAHPIRPDMV IEMNNFYTLT VYEKGAEVIR MLHTLLGEEN FQKGMQLYFE RHDGSAATCD DFVQAMEDAS NVDLSHFRLW YSQSGTPIVT VHDDYNPETE QYTLTISQRT PPTAEQAEKQ PLHIPFAIEL YDNEGKVIPL QKGGHPVHPV LNVTQAEQTF VFDNVYFQPV PALLCEFSAP VKLEYKWSDQ QLTFLMRHAR NDFSRWDAAQ SLLATYIKLN VNRHQQGQPL SLPVHVADAF RAILLDEKID PALAAEILTL PSANEIAEMF AIIDPIAIAA VREALTRTLA NELADEFLAV YNANKLDSYR VEHADIGKRA LRNTCLRYLA FAEPTLGDKL VATQYHQADN MTDALAALSA AVAAELPCRD ALMQEYDDKW HQDGLVMDKW FILQSTSPAA NVVETVRGLL NHRSFSMSNP NRVRSLIGAF ASSNPAAFHA EDGSGYQFLV EMLTELNQRN PQVASRLIEP LIRLKRYDEK RQALMRAALE QLKGLENLSG DLFEKISKAL A

NT Sequence

ATGACACAAC AGCCCCAAGC CAAATACCGC CACGACTATC GTGCGCCGGA GTATCTGATT AGCGATATCG ATTTGACCTT TGACCTGGAT GCCGCAAAAA CCGTTGTTAC GGCCGAGAGC AAGGTCTCCC GCCACGCGGC TGCGTCTGAT GTGCCATTGC GCCTGGACGG CGAAGATCTG ACCCTGATCG CTCTGCAGGT GAACGGCCAG CCGTGGAGTG ACTATAAAGA AGAAAACAAT CAGCTGGTGA TCGGCGGCCT GCCGGAGCAT TTCACCCTGA CCATCGTCAA TGAGATCAGC CCGGCGGCGA ACACCGCGCT GGAAGGCCTG TATCAGTCAG GCGAAGCGCT GTGCACCCAG TGCGAAGCGG AAGGTTTCCG CCATATCACC TGGTATCTGG ACCGTCCGGA TGTGCTGGCG CGCTTTACCA CCAAAATTAT CGCCGATAAA GCGAAATACC CGTTCCTGCT TTCCAACGGC AACCGTATGG CGGAAGGGGA GCTGGAGAAC GGTCGTCACT GGGTACAGTG GCAGGATCCG TTCCCGAAGC CGTGCTATCT GTTTGCGCTG GTGGCCGGTG ATTTTGACGT CCTGCGCGAT TCATTCCGCA CCCGCTCTGG CCGCGAAGTG GCGCTGGAGC TGTACGTCGA TCGCGGCAAC CTCGATCGCG CGCCCTGGGC GATGACCTCG CTGAAAAACT CTATGAAGTG GGATGAGGAG CGCTTTGGCC TCGAGTACGA CCTCGATATC TATATGATTG TCGCCGTCGA CTTCTTTAAT ATGGGTGCCA TGGAGAACAA AGGACTCAAC GTCTTTAACT CCAAATATGT TCTGGCTCGT ACCGATACCG CCACCGATAA AGACTACCTC GATATTGAGC GCGTGATTGG TCATGAATAT TTCCATAACT GGACCGGCAA CCGCGTCACC TGTCGTGACT GGTTCCAGCT CAGCCTGAAA GAGGGGCTGA CCGTGTTCCG CGATCAGGAG TTCAGTTCCG ACCTCGGCTC ACGGGCGGTG AACCGCATCA ACAACGTGCG CACCATGCGC GGGCTGCAGT TTGCCGAAGA CGCCAGCCCG ATGGCGCACC CGATCCGTCC GGATATGGTT ATCGAGATGA ACAACTTCTA CACCCTGACG GTCTATGAAA AGGGTGCCGA AGTGATCCGC ATGTTGCACA CCCTGCTGGG TGAAGAAAAC TTCCAGAAAG GGATGCAGCT GTACTTTGAA CGCCATGACG GCAGCGCCGC CACCTGCGAC GATTTCGTGC AGGCGATGGA AGATGCCTCT AACGTCGACC TGTCGCACTT CCGCCTCTGG TACAGCCAGT CCGGTACGCC AATCGTCACC GTGCATGACG ACTATAATCC GGAGACCGAG CAGTATACGT TGACCATCAG CCAGCGTACG CCGCCGACCG CCGAGCAGGC CGAGAAGCAG CCGCTGCATA TCCCGTTTGC CATTGAGCTG TACGATAACG AGGGCAAGGT GATCCCCCTG CAGAAGGGCG GTCACCCGGT ACACCCGGTG CTTAATGTGA CCCAGGCCGA GCAGACTTTC GTCTTCGATA ACGTTTATTT CCAGCCGGTG CCGGCGCTGC TGTGCGAATT TTCAGCGCCT GTGAAACTGG AATACAAGTG GAGCGATCAG CAGCTGACCT TCCTGATGCG TCACGCGCGG AATGATTTCT CGCGCTGGGA TGCGGCGCAG AGCCTGCTGG CGACTTACAT CAAGCTGAAC GTCAACCGCC ATCAGCAGGG GCAGCCGCTG TCGCTGCCGG TGCACGTAGC GGATGCCTTC CGCGCCATCC TGCTGGATGA GAAGATTGAT CCGGCGCTGG CCGCTGAGAT CTTAACCCTG CCGTCAGCCA ACGAAATCGC CGAGATGTTC GCGATTATCG ATCCGATCGC CATCGCCGCC GTGCGCGAAG CGCTCACCCG CACCCTGGCG AACGAACTGG CGGACGAATT CCTCGCGGTT TATAACGCCA ATAAGCTCGA CAGCTATCGG GTGGAGCACG CCGATATTGG TAAGCGCGCT CTGCGCAATA CCTGCCTGCG CTATCTGGCG TTTGCGGAAC CGACGCTGGG CGATAAGCTG GTGGCGACGC AGTATCACCA GGCAGATAAC ATGACCGACG CGCTGGCCGC CCTGTCAGCG GCGGTTGCCG CTGAGTTGCC GTGCCGCGAT GCGCTGATGC AGGAGTACGA TGACAAATGG CATCAGGACG GTCTGGTGAT GGATAAGTGG TTTATCCTGC AGTCCACCAG CCCGGCAGCC AACGTGGTGG AAACCGTGCG CGGTCTGCTG AACCACCGTT CGTTCAGCAT GAGCAACCCG AACCGTGTGC GCTCGTTAAT CGGCGCTTTC GCCAGCAGCA ACCCGGCGGC GTTCCATGCT GAAGATGGCA GCGGCTATCA GTTCCTGGTG GAGATGCTGA CCGAGCTTAA CCAGCGTAAC CCGCAGGTGG CGTCGCGCCT GATTGAGCCG TTGATTCGTC TGAAGCGTTA TGATGAGAAG CGACAGGCGC TGATGCGCGC CGCGCTGGAG CAGCTGAAGG GGCTGGAGAA TCTCTCTGGC GACCTGTTCG AGAAAATCAG TAAGGCGCTG GCG

Details for KlpnC.18101.a.B1.GE44338

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	100kDa
OBSERVED MW:	100kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	91
PERCENT COVERAGE:	34

Validated AA Sequence

MAHHHHHHMT QQPQAKYRHD YRAPEYLISD IDLSFDLDAA KTVVTAESKV SRHAAASDVP LRLDGEDLTL IALQVNGQPW SDYKEENNQL VIGGLPEHFT LTIVNEISPA ANTALEGLYQ SGEALCTQCE AEGFRHITWY LDRPDVLARF TTKIIADKAK YPFLLSNGNR MAEGELENGR HWVQWQDPFP KPCYLFALVX GDFDVLRDSF RTRSGREMAL ELYVDRGNLD RAPWAMTSLK NSMKWDXERF GLXYXLXIYM IVXXXXFNMG AXENKXLTXL LXYVLARTDT APIXTTSXXS A

Validated NT Sequence

acgactncnn tntagggaga ccacnaacgg tttccctcta gaaataattt tgtttaactt tancnaagga gatataccat ggctcaccac caccaccacc atatgacaca acagccccaa gccaaatacc gccacgacta tcgtgcgccg gagtatctga ttagcgatat cgatttgtcc tttgacctgg atgccgcaaa aaccgttgtt acggccgaga gcaaggtctc ccgccacgcg gctgcgtctg atgtgccatt gcgcctggac ggcgaagatc tgaccctgat cgctctgcag gtgaacggcc agccgtggag tgactataaa gaagaaaaca atcagctggt gatcggcggc ctgccggagc atttcaccct gaccatcgtc aatgagatca gcccggcggc gaacaccgcg ctggaaggcc tgtatcagtc aggcgaagcg ctgtgcaccc agtgcgaagc ggaaggtttc cgccatatca cctggtatct ggaccgtccg gatgtgctgg cgcgctttac caccaaaatt atcgccgata aagcgaaata cccgttcctg ctttccaacg gcaaccgtat ggcggaaggg gagctggaga atggtcgtca ctgggtacag tggcaggatc cgttcccgaa gccgtgctac ctgtttgcgc tggtggncgg tgattttgac gtcctgcgcg attcattccg cacccgctct ggccgcgaaa tggcgctgga gctgtacgtc gatcgcggca acctcgatcg cgcgccctgg gcgatgacct cgctgaaaaa ctctatgaaa tgggatgang agcgctttgg cctcnagtac nanctcnata tctatatgat tgtcnccgnc ganntcttta atatgggngc cntggagaac aaangactca cgnctttact cnnatatgtt ctggctcgta ccgataccgc nccnataaan actacctcgn nnntgagcgc gtgattggtc atgaanattt ccanaanctg gaccggcaac cnnntcnncn tgtcgtgact ggttccagct caaccctgaa anannggctg annnngttcc nnngatcnng nanntnnnnt nnnnnc

Expected Protein Sequence

MAHHHHHHMT QQPQAKYRHD YRAPEYLISD IDLTFDLDAA KTVVTAESKV SRHAAASDVP LRLDGEDLTL IALQVNGQPW SDYKEENNQL VIGGLPEHFT LTIVNEISPA ANTALEGLYQ SGEALCTQCE AEGFRHITWY LDRPDVLARF TTKIIADKAK YPFLLSNGNR MAEGELENGR HWVQWQDPFP KPCYLFALVA GDFDVLRDSF RTRSGREVAL ELYVDRGNLD RAPWAMTSLK NSMKWDEERF GLEYDLDIYM IVAVDFFNMG AMENKGLNVF NSKYVLARTD TATDKDYLDI ERVIGHEYFH NWTGNRVTCR DWFQLSLKEG LTVFRDQEFS SDLGSRAVNR INNVRTMRGL QFAEDASPMA HPIRPDMVIE MNNFYTLTVY EKGAEVIRML HTLLGEENFQ KGMQLYFERH DGSAATCDDF VQAMEDASNV DLSHFRLWYS QSGTPIVTVH DDYNPETEQY TLTISQRTPP TAEQAEKQPL HIPFAIELYD NEGKVIPLQK GGHPVHPVLN VTQAEQTFVF DNVYFQPVPA LLCEFSAPVK LEYKWSDQQL TFLMRHARND FSRWDAAQSL LATYIKLNVN RHQQGQPLSL PVHVADAFRA ILLDEKIDPA LAAEILTLPS ANEIAEMFAI IDPIAIAAVR EALTRTLANE LADEFLAVYN ANKLDSYRVE HADIGKRALR NTCLRYLAFA EPTLGDKLVA TQYHQADNMT DALAALSAAV AAELPCRDAL MQEYDDKWHQ DGLVMDKWFI LQSTSPAANV VETVRGLLNH RSFSMSNPNR VRSLIGAFAS SNPAAFHAED GSGYQFLVEM LTELNQRNPQ VASRLIEPLI RLKRYDEKRQ ALMRAALEQL KGLENLSGDL FEKISKALA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac aacagcccca agccaaatac cgccacgact atcgtgcgcc ggagtatctg attagcgata tcgatttgac ctttgacctg gatgccgcaa aaaccgttgt tacggccgag agcaaggtct cccgccacgc ggctgcgtct gatgtgccat tgcgcctgga cggcgaagat ctgaccctga tcgctctgca ggtgaacggc cagccgtgga gtgactataa agaagaaaac aatcagctgg tgatcggcgg cctgccggag catttcaccc tgaccatcgt caatgagatc agcccggcgg cgaacaccgc gctggaaggc ctgtatcagt caggcgaagc gctgtgcacc cagtgcgaag cggaaggttt ccgccatatc acctggtatc tggaccgtcc ggatgtgctg gcgcgcttta ccaccaaaat tatcgccgat aaagcgaaat acccgttcct gctttccaac ggcaaccgta tggcggaagg ggagctggag aacggtcgtc actgggtaca gtggcaggat ccgttcccga agccgtgcta tctgtttgcg ctggtggccg gtgattttga cgtcctgcgc gattcattcc gcacccgctc tggccgcgaa gtggcgctgg agctgtacgt cgatcgcggc aacctcgatc gcgcgccctg ggcgatgacc tcgctgaaaa actctatgaa gtgggatgag gagcgctttg gcctcgagta cgacctcgat atctatatga ttgtcgccgt cgacttcttt aatatgggtg ccatggagaa caaaggactc aacgtcttta actccaaata tgttctggct cgtaccgata ccgccaccga taaagactac ctcgatattg agcgcgtgat tggtcatgaa tatttccata actggaccgg caaccgcgtc acctgtcgtg actggttcca gctcagcctg aaagaggggc tgaccgtgtt ccgcgatcag gagttcagtt ccgacctcgg ctcacgggcg gtgaaccgca tcaacaacgt gcgcaccatg cgcgggctgc agtttgccga agacgccagc ccgatggcgc acccgatccg tccggatatg gttatcgaga tgaacaactt ctacaccctg acggtctatg aaaagggtgc cgaagtgatc cgcatgttgc acaccctgct gggtgaagaa aacttccaga aagggatgca gctgtacttt gaacgccatg acggcagcgc cgccacctgc gacgatttcg tgcaggcgat ggaagatgcc tctaacgtcg acctgtcgca cttccgcctc tggtacagcc agtccggtac gccaatcgtc accgtgcatg acgactataa tccggagacc gagcagtata cgttgaccat cagccagcgt acgccgccga ccgccgagca ggccgagaag cagccgctgc atatcccgtt tgccattgag ctgtacgata acgagggcaa ggtgatcccc ctgcagaagg gcggtcaccc ggtacacccg gtgcttaatg tgacccaggc cgagcagact ttcgtcttcg ataacgttta tttccagccg gtgccggcgc tgctgtgcga attttcagcg cctgtgaaac tggaatacaa gtggagcgat cagcagctga ccttcctgat gcgtcacgcg cggaatgatt tctcgcgctg ggatgcggcg cagagcctgc tggcgactta catcaagctg aacgtcaacc gccatcagca ggggcagccg ctgtcgctgc cggtgcacgt agcggatgcc ttccgcgcca tcctgctgga tgagaagatt gatccggcgc tggccgctga gatcttaacc ctgccgtcag ccaacgaaat cgccgagatg ttcgcgatta tcgatccgat cgccatcgcc gccgtgcgcg aagcgctcac ccgcaccctg gcgaacgaac tggcggacga attcctcgcg gtttataacg ccaataagct cgacagctat cgggtggagc acgccgatat tggtaagcgc gctctgcgca atacctgcct gcgctatctg gcgtttgcgg aaccgacgct gggcgataag ctggtggcga cgcagtatca ccaggcagat aacatgaccg acgcgctggc cgccctgtca gcggcggttg ccgctgagtt gccgtgccgc gatgcgctga tgcaggagta cgatgacaaa tggcatcagg acggtctggt gatggataag tggtttatcc tgcagtccac cagcccggca gccaacgtgg tggaaaccgt gcgcggtctg ctgaaccacc gttcgttcag catgagcaac ccgaaccgtg tgcgctcgtt aatcggcgct ttcgccagca gcaacccggc ggcgttccat gctgaagatg gcagcggcta tcagttcctg gtggagatgc tgaccgagct taaccagcgt aacccgcagg tggcgtcgcg cctgattgag ccgttgattc gtctgaagcg ttatgatgag aagcgacagg cgctgatgcg cgccgcgctg gagcagctga aggggctgga gaatctctct ggcgacctgt tcgagaaaat cagtaaggcg ctggcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t