KlpnC.18004.a

L-aspartate oxidase

CENTER ID: KlpnC.18004.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.18004.a.B1.GE44336 Full length(KlpnC.18004.a) 1 539
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3876
UniProt: A0A0H3H1L7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTTPDFSCD VLIIGSGAAG LSLALRLAEH SSVTVLSKGP ISEGSTFYAQ GGIAAVFDET DSIESHVEDT LIAGAGLCDR HAVTFVASNA RSCVQWLIDQ GVLFDTQVQA NGEESYHLTR EGGHSHRRIL HAADATGKAV ETTLVDKALA HPNIRILERS NAVDLIVSDK IGLPGTRRVV GAWIWNRNKE RVETCSAKAV VLATGGAAKV YQYTTNPDVS SGDGIAMAWR AGCRVANLEF NQFHPTALYH PQARNFLLTE ALRGEGAHLK RPDGTRFMPD FDERGELAPR DIVARAIDHE MKRLGVDCMF LDISHKPEAF VRQHFPMIYE KLLGLGIDLT KEPVPVVPAA HYTCGGVMVD DNGRTDVDGL YAIGEVSYTG LHGANRMASN SLLECLVYGW SAAEDITRRL PLAQKVATLP AWDESQVEIP DELVVIQHNW HELRLLMWDY VGIVRTTRRL ERALRRITML QQELDEYYAR FRVSNNLLEL RNLVQVAELI VRCAMLRKES RGLHYTLDYP QPLPDSGPSI LSPLAHIKR
NT Sequence
ATGAATACCA CTCCTGACTT CTCTTGTGAT GTGTTGATTA TCGGCAGCGG CGCTGCCGGT CTTTCCCTTG CCCTGCGCCT GGCTGAGCAC AGTTCGGTGA CCGTGCTGAG TAAAGGGCCG ATCAGCGAAG GTTCGACGTT CTACGCCCAG GGTGGGATTG CCGCCGTTTT CGACGAGACG GACAGTATTG AATCCCATGT CGAAGATACC CTGATTGCCG GCGCAGGGTT ATGCGATCGC CATGCGGTCA CCTTCGTCGC CAGCAATGCC AGATCATGCG TCCAGTGGCT TATCGATCAG GGCGTATTGT TTGACACTCA GGTCCAGGCC AATGGCGAAG AGAGCTATCA TCTCACGCGC GAAGGGGGCC ACAGCCATCG GCGCATTCTC CACGCCGCCG ACGCTACCGG CAAAGCGGTG GAGACGACGC TGGTCGATAA AGCCCTCGCC CACCCCAATA TCCGCATCCT TGAGCGCAGC AATGCCGTCG ACTTGATCGT ATCGGATAAG ATTGGCTTGC CAGGCACGCG CCGGGTGGTC GGGGCATGGA TCTGGAATCG TAATAAAGAG CGGGTGGAAA CCTGCAGCGC GAAAGCCGTA GTACTCGCTA CCGGCGGGGC CGCGAAGGTT TACCAATATA CCACTAACCC CGACGTCTCT TCCGGAGACG GGATTGCGAT GGCCTGGCGG GCGGGATGTC GGGTGGCGAA TCTGGAGTTT AACCAGTTCC ACCCCACCGC GCTATATCAC CCCCAGGCGC GTAATTTCCT GCTTACTGAA GCGCTGCGCG GCGAAGGCGC CCACCTTAAA CGCCCGGATG GCACCCGCTT TATGCCGGAC TTTGACGAGC GCGGCGAGCT GGCCCCGCGC GATATCGTCG CCCGCGCCAT CGACCATGAA ATGAAGCGCC TGGGCGTAGA CTGTATGTTC CTTGATATCA GCCATAAACC CGAGGCGTTT GTTCGCCAGC ATTTCCCGAT GATTTATGAA AAGCTGCTCG GCCTGGGAAT TGATTTAACC AAAGAGCCGG TGCCGGTCGT CCCCGCCGCG CATTACACCT GCGGCGGCGT GATGGTGGAT GATAATGGCC GCACCGACGT CGATGGTTTA TACGCCATCG GCGAAGTCAG CTACACCGGG CTGCACGGCG CGAATCGTAT GGCGTCGAAC TCGTTGCTGG AGTGCCTGGT CTATGGCTGG TCCGCCGCGG AGGATATTAC CCGGCGCCTG CCGCTCGCCC AGAAGGTCGC CACGCTGCCA GCATGGGATG AAAGTCAGGT GGAGATCCCG GATGAACTGG TGGTTATCCA GCACAACTGG CACGAACTGC GCCTGCTGAT GTGGGATTAC GTGGGGATTG TGCGCACCAC CCGCAGGCTG GAGCGCGCCC TGCGGCGCAT TACCATGCTC CAGCAGGAGC TGGATGAGTA TTACGCCCGC TTCCGCGTCT CGAATAATTT GCTGGAACTG CGCAATCTGG TGCAGGTGGC GGAGCTTATC GTACGCTGCG CGATGTTGCG TAAGGAGAGC CGCGGTCTGC ATTATACCCT CGACTATCCG CAGCCGCTGC CTGACAGCGG CCCGTCGATA CTCTCGCCCC TGGCTCACAT AAAGAGA
Details for KlpnC.18004.a.B1.GE44336
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 61kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN TTPDFSCDVL IIGSGAAGLS LALRLAEHSS VTVLSKGPIS EGSTFYAQGG IAAVFDETDS IESHVEDTLI AGAGLCDRHA VTFVASNARS CVQWLIDQGV LFDTQVQANG EESYHLTREG GHSHRRILHA ADATGKAVET TLVDKALAHP NIRILERSNA VDLIVSDXXG LPGTRRVVGA WIWNXNKXRV ETCSAKAVVL ATGGAAXVYX YTXNPDVSSX XXIAXAWRAG CXVANLEFNQ FHPTALYHPQ ARNFLLTEAL RGEGAXXKRP DGTRFMPDFD ERGELAPRDI VARAIDHEMK RLGVDCMFLD ISHKPEAFVR QHFPMIYEKL LGLGIDLXKE PVPVVPAAHY TCGGVMVDDN GRTDVDGLYA IGEVSYTGLH GANRMASNSL LECLVYGWSA AEDIARRLPL AQKVATLPAW DESQVEIPDE LVVIQHNWHE LRLLMWDYVG IVRTTRRLER ALRRITMLQQ ELDEYYARFR VSNNLLELRN LVQVAELIVR CAMLRKESRG LHYTLDYPQP LPDSGPSILS
Validated NT Sequence
gcganagtat cgacgggccg ctgtcaggca gcggctgcgg atagtcgagg gtataatgca gaccgcggct ctccttacgc aacatcgcgc agcgtacgat aagctccgcc acctgcacca gattgcgcag ttccagcaaa ttattcgaga cgcggaagcg ggcgtaatac tcatccagct cctgctggag catggtaatg cgccgcaggg cgcgctccag cctgcgggtg gtgcgcacaa tccccacgta atcccacatc agcaggcgca gttcgtgcca gttgtgctgg ataaccacca gttcatccgg gatctccacc tgactttcat cccatgctgg cagcgtggcg accttctggg cgagcggcag gcgccgggca atatcctccg cggcggacca gccatagacc aggcactcca gcaacgagtt cgacgccata cgattcgcgc cgtgcagccc ggtgtagcta acttcgccga tggcgtataa accatcgacg tcggtgcggc cattatcatc caccatcacg ccgccgcagg tgtaatgcgc ggcggggacg accggcaccg gctctttgnt taaatcaatt cccaggccga gcagcttttc ataaatcatc gggaaatgct ggcgaacaaa cgcctcgggt ttatggctga tatcaaggaa catacagtct acgcccaggc gcttcatttc atggtcgatg gcgcgggcga cgatatcgcg cggggccagc tcgccgcgct cgtcaaagtc cggcataaag cgggtgccat ccgggcgttt annnngggcg ccttcgccgc gcagcgcttc agtaagcagg aaattacgcg cctgggggtg atatagcgcg gtggggtgga actggttaaa ctccagattc gccaccngac atcccgcccg ccaggccntc gcaatccnnt ntcnggaaga gacgtcgggg ttagnggtat atngataaac nttcgcggcc ccgccggtag cgagtactac ggctttcgcg ctgcaggttt ccacccgntc tttattanga ttccagatcc atgccccgac cacccggcgg gtgcccggca ggccantntt atccgatacg attaaatcga cggcattgct gcgctcaagg atgcggatat taggatgggc aagcgcttta tcgaccagcg tngtctctac cgctttgccg gtggcgtcgg cggcgtgaag aatgcgccga tggctgtgcc ccccttcgcg cgtgagatga tagctctctt cgccattggc ctggacctgg gtgtcaaaca gcactccctg atcgataagc cactggacgc atgatcgggc gttgctggcg acgaaggtga ccgcatgacg atcgcagagc cccgcgccgg cgatgagggt atcttcgaca tgggattcaa tactgtccgt ctcatcgaag acggcggcaa tcccaccctg ggcgtagaac gtcgaacctt cgctgatcgg ccctttactc agcacggtca ccgaactgtg ctcagccagg cgcagggcaa gggaaagacc ggcagcgccg ctgccgataa tcaacacatc acaagagaag tcaggagtgg tattcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMN TTPDFSCDVL IIGSGAAGLS LALRLAEHSS VTVLSKGPIS EGSTFYAQGG IAAVFDETDS IESHVEDTLI AGAGLCDRHA VTFVASNARS CVQWLIDQGV LFDTQVQANG EESYHLTREG GHSHRRILHA ADATGKAVET TLVDKALAHP NIRILERSNA VDLIVSDKIG LPGTRRVVGA WIWNRNKERV ETCSAKAVVL ATGGAAKVYQ YTTNPDVSSG DGIAMAWRAG CRVANLEFNQ FHPTALYHPQ ARNFLLTEAL RGEGAHLKRP DGTRFMPDFD ERGELAPRDI VARAIDHEMK RLGVDCMFLD ISHKPEAFVR QHFPMIYEKL LGLGIDLTKE PVPVVPAAHY TCGGVMVDDN GRTDVDGLYA IGEVSYTGLH GANRMASNSL LECLVYGWSA AEDITRRLPL AQKVATLPAW DESQVEIPDE LVVIQHNWHE LRLLMWDYVG IVRTTRRLER ALRRITMLQQ ELDEYYARFR VSNNLLELRN LVQVAELIVR CAMLRKESRG LHYTLDYPQP LPDSGPSILS PLAHIKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata ccactcctga cttctcttgt gatgtgttga ttatcggcag cggcgctgcc ggtctttccc ttgccctgcg cctggctgag cacagttcgg tgaccgtgct gagtaaaggg ccgatcagcg aaggttcgac gttctacgcc cagggtggga ttgccgccgt tttcgacgag acggacagta ttgaatccca tgtcgaagat accctgattg ccggcgcagg gttatgcgat cgccatgcgg tcaccttcgt cgccagcaat gccagatcat gcgtccagtg gcttatcgat cagggcgtat tgtttgacac tcaggtccag gccaatggcg aagagagcta tcatctcacg cgcgaagggg gccacagcca tcggcgcatt ctccacgccg ccgacgctac cggcaaagcg gtggagacga cgctggtcga taaagccctc gcccacccca atatccgcat ccttgagcgc agcaatgccg tcgacttgat cgtatcggat aagattggct tgccaggcac gcgccgggtg gtcggggcat ggatctggaa tcgtaataaa gagcgggtgg aaacctgcag cgcgaaagcc gtagtactcg ctaccggcgg ggccgcgaag gtttaccaat ataccactaa ccccgacgtc tcttccggag acgggattgc gatggcctgg cgggcgggat gtcgggtggc gaatctggag tttaaccagt tccaccccac cgcgctatat cacccccagg cgcgtaattt cctgcttact gaagcgctgc gcggcgaagg cgcccacctt aaacgcccgg atggcacccg ctttatgccg gactttgacg agcgcggcga gctggccccg cgcgatatcg tcgcccgcgc catcgaccat gaaatgaagc gcctgggcgt agactgtatg ttccttgata tcagccataa acccgaggcg tttgttcgcc agcatttccc gatgatttat gaaaagctgc tcggcctggg aattgattta accaaagagc cggtgccggt cgtccccgcc gcgcattaca cctgcggcgg cgtgatggtg gatgataatg gccgcaccga cgtcgatggt ttatacgcca tcggcgaagt cagctacacc gggctgcacg gcgcgaatcg tatggcgtcg aactcgttgc tggagtgcct ggtctatggc tggtccgccg cggaggatat tacccggcgc ctgccgctcg cccagaaggt cgccacgctg ccagcatggg atgaaagtca ggtggagatc ccggatgaac tggtggttat ccagcacaac tggcacgaac tgcgcctgct gatgtgggat tacgtgggga ttgtgcgcac cacccgcagg ctggagcgcg ccctgcggcg cattaccatg ctccagcagg agctggatga gtattacgcc cgcttccgcg tctcgaataa tttgctggaa ctgcgcaatc tggtgcaggt ggcggagctt atcgtacgct gcgcgatgtt gcgtaaggag agccgcggtc tgcattatac cctcgactat ccgcagccgc tgcctgacag cggcccgtcg atactctcgc ccctggctca cataaagaga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat