KlpnC.17955.a

DNA polymerase III subunit delta

CENTER ID: KlpnC.17955.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.17955.a.B1.GE44335 Full length(KlpnC.17955.a) 1 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.1471
UniProt: A0A0H3GTY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYPEQLRAQL TEGLRAAYLL LGNDPLLLQE SQDAIREAAA AQGFTEHHTF SIDNSTDWQA IFALSQAMSL FASRQTLLLI LPENGPNAAI NEQLATLVGL LHDDLLLIVR GNKLTKAQEN AAWLTALAQR AVQVSCQTPE YAQLPRWLAA RAKQHQLQLD DAASQLLCYC YEGNLLALAQ ALERLALLWP DGKLTLPRVE QAVNDAAHFT PYHWVDALLA GKSKRVLHVL QQLRLEGCEP AILLRTLQRE LLLLVTLKRQ ATHTPLRSLF DKHRVWQNRR QLLSDALTRL SGEQLRQAVT LLTRAELTFK QDYGHDVWPE LESLSLLLCH KALADVFIDG
NT Sequence
TTGTACCCGG AACAGCTCCG CGCGCAGCTC ACTGAAGGGC TGCGCGCGGC CTATCTGCTG CTTGGCAACG ATCCCCTGCT GTTGCAGGAA AGCCAGGACG CCATTCGCGA AGCTGCTGCC GCGCAGGGCT TTACCGAGCA CCATACTTTT TCAATCGACA ACAGCACCGA CTGGCAGGCG ATTTTCGCCT TAAGCCAGGC GATGAGCCTG TTTGCCAGCC GGCAAACCCT GCTGTTGATC CTACCGGAAA ACGGCCCCAA CGCGGCGATC AATGAACAGC TCGCCACGCT GGTTGGCCTG CTGCACGACG ACCTGCTGCT GATAGTTCGC GGAAATAAGC TGACCAAAGC CCAGGAGAAT GCCGCATGGC TCACCGCCCT GGCTCAGCGC GCCGTCCAGG TCAGTTGCCA GACGCCGGAA TACGCGCAGC TGCCGCGCTG GCTGGCCGCC CGGGCCAAAC AGCATCAGCT GCAGCTCGAC GACGCGGCCA GTCAGCTGCT GTGCTACTGT TACGAAGGCA ATCTGCTGGC GCTGGCGCAG GCGCTGGAGC GTCTGGCGCT GCTCTGGCCG GACGGTAAGC TAACCCTCCC CCGCGTTGAG CAAGCGGTAA ACGATGCCGC GCACTTTACG CCTTACCATT GGGTCGATGC CCTACTGGCA GGCAAAAGTA AGCGCGTGCT GCACGTCCTG CAGCAGTTAC GCCTTGAAGG CTGCGAGCCG GCGATCCTGC TGCGTACTCT GCAGCGGGAG CTGCTGCTGC TGGTTACCCT CAAGCGCCAG GCGACGCACA CGCCGCTGCG CTCGCTGTTT GATAAACATC GGGTCTGGCA AAACCGCCGT CAGCTGCTCA GCGACGCCCT CACGCGCCTG AGCGGGGAAC AGCTGCGCCA GGCGGTGACC CTTCTGACCC GCGCGGAGCT CACCTTTAAA CAGGATTACG GGCACGACGT CTGGCCCGAA CTGGAAAGTC TTTCGTTGTT GCTCTGCCAC AAAGCCCTGG CGGACGTTTT TATTGATGGT
Details for KlpnC.17955.a.B1.GE44335
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML YPEQLRAQLT EGLRAAYLLL GNDPLLLQES QDAIREAAAA QGFTEHHTFS IDNSTDWQAI FALSQAMSLF ASRQTLLLIL PENGPNAAIN EQLATLVGLL HDDLLLIVRG NKLSKAQENA AWLTALAQRA VQVSCQTPEY AQLPRWLAAR AKQHQLQLDD AASQLLCYCY EGNLLALAQA LERLALLWPD GKLTLPRVEQ AVNDAAHFTP YHWVDALLAG KSKRVLHVLQ QLRLEGCEPA ILLRTLQREL LLLVTLKRQA THTPLRSLFD KHRVWQNRRQ LLSDALTRLS GEQLRQAVTL LTRAELTFKQ DYGHDVWPEL ESLSLLLCXK
Validated NT Sequence
gctttgnggc agagcaacaa cgaaagactt tccagttcgg gccagacgtc gtgcccgtaa tcctgtttaa aggtgagctc cgcgcgggtc agaagggtca ccgcctggcg cagctgttcc ccgctcaggc gcgtgagggc gtcgctgagc agctgacggc ggttttgcca gacccgatgt ttatcaaaca gcgagcgcag cggcgtgtgc gtcgcctggc gcttgagggt aaccagcagc agcagctccc gctgcagagt acgcagcagg atcgccggct cgcagccttc aaggcgtaac tgctgcagga cgtgcagcac gcgcttactt ttgcctgcca gtagggcatc gacccaatgg taaggcgtaa agtgcgcggc atcgtttacc gcttgctcaa cgcggggcag ggttagctta ccgtccggcc agagcagcgc cagacgctcc agcgcctgcg ccagcgccag cagattgcct tcgtaacagt agcacagcag ctgactggcc gcgtcgtcga gctgcagctg atgctgtttg gcacgggcgg ccagccagcg cggcagctgc gcgtattccg gcgtctggca actgacctgg acggcgcgct gagccagggc ggtgagccat gcggcattct cctgggcttt ggacagctta tttccgcgaa ctatcagcag caggtcgtcg tgcagcaggc caaccagcgt ggcgagctgt tcattgatcg ccgcgttggg gccgttttcc ggtaggatca atagcagggt ttgccggctg gcaaacaggc tcatcgcctg gcttaaggcg aaaatcgcct gccagtcggt gctgttgtcg attgaaaaag tatggtgctc ggtaaagccc tgcgcggcag cagcttcgcg aatggcgtcc tggctttcct gcaacagcag gggatcgttg ccaagcagca gataggccgc gcgcagccct tcagtgagct gcgcgcggag ctgttccggg tacaacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMY PEQLRAQLTE GLRAAYLLLG NDPLLLQESQ DAIREAAAAQ GFTEHHTFSI DNSTDWQAIF ALSQAMSLFA SRQTLLLILP ENGPNAAINE QLATLVGLLH DDLLLIVRGN KLTKAQENAA WLTALAQRAV QVSCQTPEYA QLPRWLAARA KQHQLQLDDA ASQLLCYCYE GNLLALAQAL ERLALLWPDG KLTLPRVEQA VNDAAHFTPY HWVDALLAGK SKRVLHVLQQ LRLEGCEPAI LLRTLQRELL LLVTLKRQAT HTPLRSLFDK HRVWQNRRQL LSDALTRLSG EQLRQAVTLL TRAELTFKQD YGHDVWPELE SLSLLLCHKA LADVFIDG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtacc cggaacagct ccgcgcgcag ctcactgaag ggctgcgcgc ggcctatctg ctgcttggca acgatcccct gctgttgcag gaaagccagg acgccattcg cgaagctgct gccgcgcagg gctttaccga gcaccatact ttttcaatcg acaacagcac cgactggcag gcgattttcg ccttaagcca ggcgatgagc ctgtttgcca gccggcaaac cctgctgttg atcctaccgg aaaacggccc caacgcggcg atcaatgaac agctcgccac gctggttggc ctgctgcacg acgacctgct gctgatagtt cgcggaaata agctgaccaa agcccaggag aatgccgcat ggctcaccgc cctggctcag cgcgccgtcc aggtcagttg ccagacgccg gaatacgcgc agctgccgcg ctggctggcc gcccgggcca aacagcatca gctgcagctc gacgacgcgg ccagtcagct gctgtgctac tgttacgaag gcaatctgct ggcgctggcg caggcgctgg agcgtctggc gctgctctgg ccggacggta agctaaccct cccccgcgtt gagcaagcgg taaacgatgc cgcgcacttt acgccttacc attgggtcga tgccctactg gcaggcaaaa gtaagcgcgt gctgcacgtc ctgcagcagt tacgccttga aggctgcgag ccggcgatcc tgctgcgtac tctgcagcgg gagctgctgc tgctggttac cctcaagcgc caggcgacgc acacgccgct gcgctcgctg tttgataaac atcgggtctg gcaaaaccgc cgtcagctgc tcagcgacgc cctcacgcgc ctgagcgggg aacagctgcg ccaggcggtg acccttctga cccgcgcgga gctcaccttt aaacaggatt acgggcacga cgtctggccc gaactggaaa gtctttcgtt gttgctctgc cacaaagccc tggcggacgt ttttattgat ggttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat