KlpnC.17938.a

UDP-N-acetylmuramoylalanine--D-glutamate ligase

CENTER ID: KlpnC.17938.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.17938.a.B1.GE44334 Full length(KlpnC.17938.a) 1 438
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.784
UniProt: A0A0H3GML0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADYQGKKVV IIGLGMTGLS CVDFFMARGV TPRVMDTRVA PPGLDKLPES VECHVGGLND TWLLAADLIV ASPGIALAHP SLSAAADAGV EIVGDIELFC REAQAPIIAI TGSNGKSTVT TLVGEMAKAA GVNVGVGGNI GLPALMLLDT DRELYVLELS SFQLETTSSL QAVAATILNV TEDHMDRYPL GLQQYRAAKL RIYENAKTCV VNADDALTMP VRGADERCIS FGVDVGDYHL NRQQGETWLR VKGEKVLNVK EMPLTGQHNY SNALAALALA DAAGLPRASS LKALTTFTGL AHRFQLVLDH NGVRWINDSK ATNVGSTEAA LNGLQLDGTL YLLLGGDGKS ADFTPLKRYL GGDRIRLYCF GRDGAELAEL RPEVAVQTET MEQAMRQIAP LVKAGDMVLL SPACASLDQF KNFEQRGEMF ARLAKELG
NT Sequence
ATGGCAGATT ATCAGGGTAA AAAAGTCGTC ATTATTGGGC TGGGTATGAC CGGCCTGTCA TGCGTGGACT TTTTCATGGC GCGCGGCGTA ACCCCGCGCG TGATGGACAC CCGCGTCGCG CCTCCGGGGC TGGATAAATT ACCCGAATCG GTCGAATGCC ATGTCGGCGG ACTCAATGAT ACCTGGCTGC TGGCGGCGGA CCTGATTGTC GCCAGTCCGG GGATTGCGCT GGCCCATCCT TCGCTCAGCG CAGCGGCCGA CGCTGGCGTA GAGATCGTTG GCGATATCGA ACTGTTCTGC CGCGAAGCGC AGGCGCCGAT TATCGCCATT ACCGGTTCTA ACGGCAAAAG CACTGTTACT ACCCTGGTGG GCGAGATGGC GAAAGCCGCT GGAGTTAACG TCGGCGTGGG CGGCAATATC GGCCTGCCGG CGCTGATGCT GCTGGACACC GATCGAGAGC TGTATGTGCT GGAGCTCTCC AGCTTCCAGC TGGAAACCAC CTCCAGCCTG CAGGCGGTGG CGGCAACCAT CCTCAACGTG ACTGAGGACC ATATGGACCG CTACCCGCTG GGGCTGCAGC AGTATCGCGC GGCGAAGCTG CGGATTTACG AGAATGCGAA GACCTGCGTG GTGAACGCGG ACGATGCGCT CACCATGCCG GTACGCGGGG CGGACGAACG CTGCATTAGC TTTGGCGTCG ATGTCGGTGA CTACCACCTT AACCGCCAGC AGGGCGAAAC CTGGCTGCGC GTTAAAGGTG AGAAAGTCCT CAATGTGAAA GAGATGCCTC TCACCGGTCA GCATAACTAC AGCAACGCCC TGGCGGCGCT GGCGCTGGCC GATGCCGCGG GTCTGCCGCG GGCCAGCAGC CTGAAGGCGC TGACCACCTT TACCGGTCTG GCGCACCGTT TCCAGCTGGT GCTGGATCAC AACGGCGTAC GCTGGATTAA CGACTCGAAG GCGACCAACG TCGGCAGCAC CGAGGCGGCG CTGAACGGCC TGCAGCTGGA CGGCACGCTC TATCTGCTGC TGGGCGGCGA CGGCAAGTCC GCTGATTTTA CCCCGCTGAA GCGCTACCTC GGCGGCGATC GTATTCGCCT GTACTGCTTT GGTCGCGATG GCGCCGAGCT CGCTGAGCTG CGTCCTGAGG TGGCGGTACA AACTGAGACT ATGGAACAGG CGATGCGTCA GATTGCTCCC CTGGTGAAAG CGGGGGACAT GGTTCTCCTG TCGCCGGCCT GCGCTAGCCT GGATCAGTTT AAGAACTTTG AACAACGGGG CGAGATGTTT GCCCGTCTGG CGAAGGAGTT AGGT
Details for KlpnC.17938.a.B1.GE44334
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 92
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMA DYQGKKVVII GLGMTGLSCV DFFMARGVTP RVMDTRVAPP GLDKLPESVE CHVGGLNDTW LLAADLIVAS PGIALAHPSL SAAADAGVEI VGDIELFCRE AQAPIIAITG SNGKSTVTTL VGEMAKAAGV NVGVGGNIGL PALMLLDTDR ELYVLELSSF QLETTSSLQA VAATILNVTE DHMDRYPLGL QQYRAAKLRI YENAKTCVVN ADDALTMPVR GADERCIXFG VDVGDYHLNR QXGETWLRVK GEKVLXVKRC LSPXQHNXSN ALAALALADA AGLXRASXLK ALTTFTGLXX RFQLXLDHNG VRWINXSKAT NVGXXXXRXX XX
Validated NT Sequence
gnnnnnnnnn nnnnnnnnnn acgactcnct atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tggcagatta tcagggtaaa aaagtcgtca ttattgggct gggtatgacc ggcctgtcat gcgtggactt tttcatggcg cgcggcgtaa ccccgcgcgt gatggacacc cgcgtcgcgc ctccggggct ggataaatta cccgaatcgg tcgaatgcca tgtcggcgga ctcaatgata cctggctgct ggcggcggac ctgattgtcg ccagtccggg gattgcgctg gcccatcctt cgctcagcgc agcggccgac gctggcgtag agatcgttgg cgatatcgaa ctgttctgcc gcgaagcgca ggcgccgatt atcgccatta ccggttctaa cggcaaaagc actgttacta ccctggtggg cgagatggcg aaagccgctg gagttaacgt cggcgtgggc ggcaatatcg gcctgccggc gctgatgctg ctggacaccg atcgagagct gtatgtgctg gagctctcca gcttccagct ggaaaccacc tccagcctgc aggcggtggc ggcaaccatc ctcaacgtga ctgaggacca tatggaccgc tacccgctgg ggctgcagca gtatcgcgcg gcgaagctgc ggatttacga gaatgcgaag acctgcgtgg tgaacgcgga cgatgcgctc accatgccgg tacgcggggc ggacgaacgc tgcattanct ttggcgtcga tgtcggtgac taccacctta accgccagca nggcgaaacc tggctgcgcg ttaaaggtga gaaagtcctc antgtgaaaa gatgcctctc accggntcag cataactnca gcaacgccct ggcggcgctg gcgctggccg atgccgcggg tctgnnncgg gccagcancc tgaaagcgct gaccaccttt accggtctgg nnnaccgttt ccagctggng ctggatcaca acggcgtacg ctggattaac gantcgaagg cgaccaacgt cggnannncc cnanngcggn nctnaanngn ctgc
Expected Protein Sequence
MAHHHHHHMA DYQGKKVVII GLGMTGLSCV DFFMARGVTP RVMDTRVAPP GLDKLPESVE CHVGGLNDTW LLAADLIVAS PGIALAHPSL SAAADAGVEI VGDIELFCRE AQAPIIAITG SNGKSTVTTL VGEMAKAAGV NVGVGGNIGL PALMLLDTDR ELYVLELSSF QLETTSSLQA VAATILNVTE DHMDRYPLGL QQYRAAKLRI YENAKTCVVN ADDALTMPVR GADERCISFG VDVGDYHLNR QQGETWLRVK GEKVLNVKEM PLTGQHNYSN ALAALALADA AGLPRASSLK ALTTFTGLAH RFQLVLDHNG VRWINDSKAT NVGSTEAALN GLQLDGTLYL LLGGDGKSAD FTPLKRYLGG DRIRLYCFGR DGAELAELRP EVAVQTETME QAMRQIAPLV KAGDMVLLSP ACASLDQFKN FEQRGEMFAR LAKELG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag attatcaggg taaaaaagtc gtcattattg ggctgggtat gaccggcctg tcatgcgtgg actttttcat ggcgcgcggc gtaaccccgc gcgtgatgga cacccgcgtc gcgcctccgg ggctggataa attacccgaa tcggtcgaat gccatgtcgg cggactcaat gatacctggc tgctggcggc ggacctgatt gtcgccagtc cggggattgc gctggcccat ccttcgctca gcgcagcggc cgacgctggc gtagagatcg ttggcgatat cgaactgttc tgccgcgaag cgcaggcgcc gattatcgcc attaccggtt ctaacggcaa aagcactgtt actaccctgg tgggcgagat ggcgaaagcc gctggagtta acgtcggcgt gggcggcaat atcggcctgc cggcgctgat gctgctggac accgatcgag agctgtatgt gctggagctc tccagcttcc agctggaaac cacctccagc ctgcaggcgg tggcggcaac catcctcaac gtgactgagg accatatgga ccgctacccg ctggggctgc agcagtatcg cgcggcgaag ctgcggattt acgagaatgc gaagacctgc gtggtgaacg cggacgatgc gctcaccatg ccggtacgcg gggcggacga acgctgcatt agctttggcg tcgatgtcgg tgactaccac cttaaccgcc agcagggcga aacctggctg cgcgttaaag gtgagaaagt cctcaatgtg aaagagatgc ctctcaccgg tcagcataac tacagcaacg ccctggcggc gctggcgctg gccgatgccg cgggtctgcc gcgggccagc agcctgaagg cgctgaccac ctttaccggt ctggcgcacc gtttccagct ggtgctggat cacaacggcg tacgctggat taacgactcg aaggcgacca acgtcggcag caccgaggcg gcgctgaacg gcctgcagct ggacggcacg ctctatctgc tgctgggcgg cgacggcaag tccgctgatt ttaccccgct gaagcgctac ctcggcggcg atcgtattcg cctgtactgc tttggtcgcg atggcgccga gctcgctgag ctgcgtcctg aggtggcggt acaaactgag actatggaac aggcgatgcg tcagattgct cccctggtga aagcggggga catggttctc ctgtcgccgg cctgcgctag cctggatcag tttaagaact ttgaacaacg gggcgagatg tttgcccgtc tggcgaagga gttaggttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at