KlpnC.17899.a

Dihydrofolate synthase/folylpolyglutamate synthase

CENTER ID: KlpnC.17899.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.17899.a.B1.GE44332 Full length(KlpnC.17899.a) 1 422
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3678
UniProt: A0A0H3GVS7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKDPIPQAT SPLATWLSYL EHLHSKTIDL GLARVSEVAG QMDIVKPAPF VFTVAGTNGK GTTCRTLETI LMAAGYKVGV YSSPHLVRYT ERVRIQGAEL PEAAHTASFA EIEAARGDIS LTYFEYGTLS ALWLFKQAQL DVVILEVGLG GRLDATNIVD ADVAVVTSIA LDHTDWLGPD RESIGREKAG IFRAGKPAVV GEPDMPLTIA EVASEKGALL QRRGVDWRYE VEGETWSFRD TAGALSHLPL PQVPLPNAAT AVAALRASGL AVDDAILRAG IRDAMLPGRF QIISDAPRVI LDVAHNPHAA AYLAGRLKTL AKTGRVLAVI GMLHDKDIAG TLANLAPEVD AWYCAPLEGP RGATAEQLVE HLRCGTVYSS VAQAWRAAMA DAKVEDTVLV CGSFHTVAQV MEEIDAGRIG GE
NT Sequence
ATGGAAAAAG ATCCTATTCC TCAGGCCACG TCGCCCCTGG CTACGTGGCT TTCTTATCTG GAACATCTGC ACAGTAAAAC CATCGACCTT GGGCTGGCGC GCGTCAGCGA GGTCGCCGGG CAGATGGATA TCGTTAAACC GGCGCCGTTC GTTTTTACCG TTGCGGGCAC CAATGGCAAA GGCACCACCT GCCGCACGCT GGAAACCATC CTGATGGCGG CCGGTTATAA AGTGGGGGTC TACAGCTCTC CGCATCTGGT GCGCTACACC GAACGCGTGC GCATTCAGGG GGCTGAACTG CCGGAGGCGG CGCATACAGC TTCGTTTGCT GAAATCGAAG CGGCGCGGGG TGACATCTCG CTGACCTATT TTGAATACGG CACGCTCTCG GCGCTGTGGC TGTTTAAGCA GGCGCAGCTG GATGTGGTGA TCCTTGAGGT CGGGCTCGGC GGGCGTCTCG ACGCCACCAA CATCGTGGAT GCCGACGTCG CGGTGGTCAC CAGCATCGCC CTCGATCATA CCGACTGGCT GGGCCCGGAT CGCGAAAGTA TCGGCCGGGA AAAAGCGGGC ATCTTTCGCG CCGGTAAACC GGCGGTGGTG GGGGAGCCGG ATATGCCGCT CACCATCGCG GAAGTGGCTA GCGAGAAGGG CGCGCTGCTC CAGCGCCGCG GCGTCGACTG GCGCTATGAG GTGGAGGGCG AAACGTGGTC CTTCCGCGAT ACGGCAGGGG CGTTAAGCCA CCTGCCTCTG CCTCAGGTGC CGTTGCCGAA TGCGGCGACG GCCGTGGCGG CGCTGCGCGC CAGCGGGCTT GCCGTGGATG ACGCTATCCT GCGCGCAGGG ATCCGCGATG CGATGCTCCC GGGACGCTTT CAGATAATCA GCGACGCCCC GCGAGTGATC CTCGATGTGG CGCACAACCC GCACGCGGCG GCCTATCTCG CCGGGCGTCT CAAAACGTTA GCGAAAACGG GACGCGTGCT GGCGGTTATT GGTATGCTGC ATGATAAGGA TATCGCCGGC ACGTTGGCCA ATCTGGCCCC GGAAGTCGAT GCCTGGTACT GCGCGCCGCT GGAAGGCCCG CGCGGCGCGA CGGCAGAGCA GCTGGTGGAA CATTTGCGCT GCGGTACAGT CTATAGCAGC GTGGCGCAGG CGTGGCGTGC GGCCATGGCT GACGCTAAAG TGGAAGATAC CGTGCTGGTG TGTGGTTCGT TTCACACCGT AGCGCAGGTT ATGGAAGAGA TAGACGCGGG GAGAATCGGT GGCGAG
Details for KlpnC.17899.a.B1.GE44332
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 51
Validated AA Sequence
MAHHHHHHME KDPIPQATSP LATWLSYLEH LHNKTIDLGL ARVSEVAGQM DIVKPAPFVF TVAGTNGKGT TCRTLETILM AAGYKVGVYS SPHLVRYTER VRIQGAELPE AAHTASFAEI EAARGDISLT YFEYGTLSAL WLFKQAQLDV VILEVGLGGR LDATNIVDAD VAVVTSIALD HTDWLGPDRE SIGREKAGIF RAGKPAVVGE PDMPLTIAGS G
Validated NT Sequence
cgactnnntn tagggagacc acaacggttt ccctctagaa ataattttgt ttaactttaa ncaaggagat ataccatggc tcaccaccac caccaccata tggaaaaaga tcctattcct caggccacgt cgcccctggc tacgtggctt tcttatctgg aacatctgca caataaaacc atcgaccttg ggctggcgcg cgtcagcgag gtcgccgggc agatggatat cgttaaaccg gcgccgttcg tttttaccgt cgcgggcacc aatggcaaag gcaccacctg ccgcacgctg gaaaccatcc tgatggcggc cggttataaa gtgggggtct acagctctcc gcatctggtg cgctacaccg aacgcgtgcg cattcagggg gctgaactgc cggaggcggc gcatacggct tcgtttgctg aaatcgaagc ggcgcggggt gacatctcgc tgacctattt tgaatacggc acgctctcgg cgctgtggct gtttaagcag gcgcagctgg atgtggtgat ccttgaggtc gggctcggcg ggcgtctcga cgctaccaac atcgttgatg ccgacgtcgc agtggtcacc agcatcgccc tcgatcatac cgactggctg gggccggatc gcgaaagtat cggccgggaa aaagcgggca tctttcgcgc cggtaaaccg gcggtggtgg gggagccgga tatgccgctc accatcgcgg gaagtggcta gcgagaangg cgcgctgctc cagcgccgcg ggcgtcactg gcgctatgag gtggaangng aaaagtggtc cttncgcgat acngcagggg cgttaagcca cctgcctctg cctcnnngcc gttgccgaat gcggcgacgg ccgtggcggc gctgcgcgcc ancgggcttg ccgtngatga ngctatcctg cgcgcaggga tccgcgatgc gatgctcccg ggacgctttc nanantancg annccccgcg agtganncct nnnatgtggc gnnnacccgc acgcggcggc ctatctcgcn nnngtnnnna acgttnnnna ancgggaann nnnngtggng nnattgnnnn nctgccnngn naaggnnatt nnncccnnnn cnttggntc
Expected Protein Sequence
MAHHHHHHME KDPIPQATSP LATWLSYLEH LHSKTIDLGL ARVSEVAGQM DIVKPAPFVF TVAGTNGKGT TCRTLETILM AAGYKVGVYS SPHLVRYTER VRIQGAELPE AAHTASFAEI EAARGDISLT YFEYGTLSAL WLFKQAQLDV VILEVGLGGR LDATNIVDAD VAVVTSIALD HTDWLGPDRE SIGREKAGIF RAGKPAVVGE PDMPLTIAEV ASEKGALLQR RGVDWRYEVE GETWSFRDTA GALSHLPLPQ VPLPNAATAV AALRASGLAV DDAILRAGIR DAMLPGRFQI ISDAPRVILD VAHNPHAAAY LAGRLKTLAK TGRVLAVIGM LHDKDIAGTL ANLAPEVDAW YCAPLEGPRG ATAEQLVEHL RCGTVYSSVA QAWRAAMADA KVEDTVLVCG SFHTVAQVME EIDAGRIGGE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aagatcctat tcctcaggcc acgtcgcccc tggctacgtg gctttcttat ctggaacatc tgcacagtaa aaccatcgac cttgggctgg cgcgcgtcag cgaggtcgcc gggcagatgg atatcgttaa accggcgccg ttcgttttta ccgttgcggg caccaatggc aaaggcacca cctgccgcac gctggaaacc atcctgatgg cggccggtta taaagtgggg gtctacagct ctccgcatct ggtgcgctac accgaacgcg tgcgcattca gggggctgaa ctgccggagg cggcgcatac agcttcgttt gctgaaatcg aagcggcgcg gggtgacatc tcgctgacct attttgaata cggcacgctc tcggcgctgt ggctgtttaa gcaggcgcag ctggatgtgg tgatccttga ggtcgggctc ggcgggcgtc tcgacgccac caacatcgtg gatgccgacg tcgcggtggt caccagcatc gccctcgatc ataccgactg gctgggcccg gatcgcgaaa gtatcggccg ggaaaaagcg ggcatctttc gcgccggtaa accggcggtg gtgggggagc cggatatgcc gctcaccatc gcggaagtgg ctagcgagaa gggcgcgctg ctccagcgcc gcggcgtcga ctggcgctat gaggtggagg gcgaaacgtg gtccttccgc gatacggcag gggcgttaag ccacctgcct ctgcctcagg tgccgttgcc gaatgcggcg acggccgtgg cggcgctgcg cgccagcggg cttgccgtgg atgacgctat cctgcgcgca gggatccgcg atgcgatgct cccgggacgc tttcagataa tcagcgacgc cccgcgagtg atcctcgatg tggcgcacaa cccgcacgcg gcggcctatc tcgccgggcg tctcaaaacg ttagcgaaaa cgggacgcgt gctggcggtt attggtatgc tgcatgataa ggatatcgcc ggcacgttgg ccaatctggc cccggaagtc gatgcctggt actgcgcgcc gctggaaggc ccgcgcggcg cgacggcaga gcagctggtg gaacatttgc gctgcggtac agtctatagc agcgtggcgc aggcgtggcg tgcggccatg gctgacgcta aagtggaaga taccgtgctg gtgtgtggtt cgtttcacac cgtagcgcag gttatggaag agatagacgc ggggagaatc ggtggcgagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat