SSGCID: Target Page

KlpnC.17898.a

Aconitate hydratase

CENTER ID:	KlpnC.17898.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.17898.a.B1.GE44331	Full length(KlpnC.17898.a)		1	890

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.2123
UniProt:	A0A0H3GRP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSTLREASK DTLQVNDKTW HYYSLPLAEK QLGEISRLPK SLKVLMENLL RWQDGDSVTE EDIRALAGWL QQAHADREIA YRPARVLMQD FTGVPAVVDL AAMREAVKRL GGDTAKVNPL SPVDLVIDHS VTVDRFGDDE AFEDNVRLEM ERNHERYAFL RWGQQAFSRF SVVPPGTGIC HQVNLEYLGR AVWSEEVNGQ WMAWPDTLVG TDSHTTMING LGVLGWGVGG IEAEAAMLGQ PVSMLIPDVV GFKLSGKLRE GITATDLVLT VTQMLRQHGV VGKFVEFYGD GLDTLPLADR ATIANMAPEY GATCGFFPID DVTLSYMRLS GRSEEQVALV EAYAKAQGMW RQPGDEPVFT STLALDMSSV EASLAGPKRP QDRVALGDVP KAFAASGELE VNHLQRQRQP VDYTLNGHHY SLPDGAVAIA AITSCTNTSN PSVLMAAGLL AKKAVERGLQ PQPWVKASLA PGSKVVSDYL AHAGLTPYLD QLGFNLVGYG CTTCIGNSGP LPEPIEEAIK KGDLTVGAVL SGNRNFEGRI HPLVKTNWLA SPPLVVAYAL AGNMNIDLTR EPLGQGKNGE PVYLKDIWPS GEEIARAVEQ VSTEMFRKEY AEVFSGTEEW KAIKVEASDT YDWQEDSTYI RLSPFFDEMG VEPLPVEDIR GARILAMLGD SVTTDHISPA GSIKADSPAG RYLQEHGVAR RDFNSYGSRR GNHEVMMRGT FANIRIRNEM VPGVEGGMTR HLPDPEPMAI YDAAMLYKAE GTPLAVIAGK EYGSGSSRDW AAKGPRLLGI RVVIAESFER IHRSNLIGMG ILPLEFPQGV TRKTLRLTGE ERIDISNLQS LQPGATVPVT LTRADGSQEA IPCRCRIDTA TELTYYRNDG ILHYVIRNML

NT Sequence

ATGTCGTCAA CCCTACGAGA AGCCAGTAAG GACACGCTGC AGGTCAACGA TAAAACCTGG CATTACTACA GTCTGCCGTT GGCGGAGAAG CAACTGGGCG AGATCAGTCG ACTGCCCAAG TCGCTGAAGG TGCTGATGGA GAACCTGCTG CGCTGGCAGG ACGGTGACTC GGTAACTGAA GAAGATATTC GCGCCCTGGC CGGCTGGCTG CAACAGGCCC ATGCCGACCG GGAAATCGCC TACCGTCCGG CACGCGTCCT GATGCAGGAC TTTACCGGCG TGCCGGCGGT AGTGGATCTG GCCGCCATGC GCGAGGCGGT GAAACGCCTT GGCGGCGATA CGGCGAAGGT TAATCCGCTA TCGCCGGTTG ACCTGGTGAT CGACCACTCG GTGACCGTTG ACCGCTTCGG CGACGACGAG GCCTTCGAAG ACAACGTCCG CCTGGAAATG GAGCGGAACC ACGAACGCTA CGCTTTTCTG CGCTGGGGGC AGCAGGCCTT CAGCCGCTTC AGCGTGGTGC CGCCGGGCAC CGGTATTTGC CACCAGGTGA ACCTGGAATA CCTTGGCCGG GCGGTCTGGA GCGAGGAGGT GAACGGCCAG TGGATGGCGT GGCCGGATAC GCTGGTGGGC ACCGACTCCC ATACCACCAT GATTAATGGT CTTGGCGTGC TGGGCTGGGG CGTGGGCGGG ATTGAAGCGG AAGCCGCTAT GCTGGGCCAG CCGGTGTCAA TGCTGATCCC CGATGTTGTG GGCTTTAAGC TCAGCGGCAA ACTGCGCGAA GGCATCACCG CCACTGACCT GGTACTGACG GTTACCCAAA TGCTGCGTCA GCATGGCGTC GTCGGCAAGT TTGTTGAATT CTACGGTGAC GGCCTGGATA CCTTACCCCT TGCCGACAGG GCGACCATCG CCAATATGGC GCCGGAGTAT GGCGCCACCT GTGGCTTTTT CCCTATCGAT GACGTAACGC TCAGCTATAT GCGTCTCAGC GGCCGTAGCG AAGAACAGGT CGCGCTGGTG GAAGCCTACG CTAAAGCCCA GGGCATGTGG CGCCAGCCTG GCGATGAGCC GGTCTTTACC AGTACCCTGG CGCTGGATAT GAGCAGCGTC GAAGCGAGCC TCGCCGGGCC GAAGCGACCG CAGGATCGCG TGGCGCTGGG CGATGTTCCG AAAGCCTTCG CCGCCAGCGG CGAGCTGGAG GTTAACCACC TTCAGCGTCA GCGTCAGCCG GTTGACTATA CGCTGAACGG GCATCACTAT TCGCTACCGG ATGGCGCGGT GGCTATTGCA GCGATTACCT CCTGCACCAA TACCTCTAAC CCCAGCGTGT TGATGGCCGC CGGCCTGCTG GCGAAAAAAG CCGTCGAACG CGGCCTGCAG CCGCAGCCGT GGGTGAAAGC TTCTCTGGCC CCAGGCTCGA AAGTGGTGTC GGATTATCTG GCCCATGCCG GCCTGACGCC TTACCTCGAC CAGCTCGGCT TCAATCTGGT GGGATATGGT TGCACCACCT GTATTGGTAA CTCCGGTCCA TTGCCTGAAC CTATCGAAGA AGCGATCAAA AAAGGCGATC TGACCGTCGG GGCAGTGCTC TCCGGTAACC GTAACTTCGA AGGGCGTATT CATCCGCTGG TGAAAACCAA CTGGCTGGCC TCGCCGCCGT TGGTGGTCGC CTATGCGCTG GCGGGGAACA TGAATATCGA CCTCACCCGC GAGCCGTTGG GACAGGGCAA GAATGGCGAG CCGGTCTATC TGAAAGATAT CTGGCCGAGC GGGGAGGAGA TTGCCCGCGC GGTGGAACAG GTCTCCACCG AGATGTTCCG CAAAGAGTAC GCGGAAGTGT TTTCCGGCAC TGAGGAGTGG AAGGCGATCA AGGTGGAGGC GTCAGATACC TACGACTGGC AGGAGGACTC GACCTATATT CGCTTGTCGC CGTTCTTTGA CGAGATGGGC GTCGAGCCGC TGCCGGTAGA GGATATTCGC GGCGCGCGGA TCCTCGCCAT GCTGGGCGAC TCAGTCACCA CGGACCACAT TTCGCCGGCG GGGAGCATCA AAGCCGATAG TCCGGCAGGC CGCTATCTGC AGGAGCATGG GGTTGCCCGG CGCGACTTTA ACTCCTATGG TTCCCGTCGC GGTAACCATG AGGTGATGAT GCGTGGCACC TTCGCCAATA TCCGCATCCG TAACGAAATG GTCCCAGGGG TGGAAGGGGG TATGACCCGC CATCTGCCGG ATCCTGAACC GATGGCGATT TACGATGCGG CGATGCTGTA CAAGGCTGAG GGCACACCGC TGGCGGTGAT TGCCGGTAAG GAGTATGGCT CAGGTTCCAG CCGTGACTGG GCGGCGAAGG GGCCGCGCCT GCTGGGGATT CGGGTAGTGA TCGCCGAGTC CTTCGAGCGT ATCCATCGTT CCAACCTGAT CGGAATGGGC ATTTTGCCGC TGGAGTTCCC GCAGGGCGTC ACCCGCAAAA CGCTACGGTT GACCGGGGAA GAGCGGATTG ATATCAGTAA TCTGCAGTCG CTTCAGCCTG GCGCTACGGT GCCGGTGACC CTGACTCGCG CTGACGGCTC GCAGGAAGCG ATCCCTTGTC GGTGCCGTAT CGACACGGCG ACAGAACTCA CCTACTACCG CAACGACGGC ATCCTGCACT ACGTTATTCG TAATATGCTG

Details for KlpnC.17898.a.B1.GE44331

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	99kDa
OBSERVED MW:	99kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	30

Validated AA Sequence

Validated NT Sequence

gnnnnnnnnn nnnnnnnnnn nactcnctat agggagacca caacggtttc cctctagaaa taattttgtt taactttaan aaggagatat accatggctc accaccacca ccaccatatg tcgtcaaccc tacgagaagc cagtaaggac acgctgcagg tcaacgataa aacctggcat tactacagtc tgccgttggc ggagaagcag ctgggcgaga tcagtcgact gcccaagtcg ctgaaggtgc tgatggagaa cctgctgcgc tggcaggacg gtgactcggt aactgaagaa gatattcgcg ccctggccgg ctggctgcaa caggcccatg ccgaccggga aatcgcctac cgtccggcac gcgtcctgat gcaggacttt accggcgtgc cggcggtagt ggatctggcc gccatgcgcg aagcggtgaa acgccttggc ggcgatacgg cgaaggttaa tccgctatcg ccggttgacc tggtgatcga ccactcggtg accgttgacc gcttcggcga cgacgaggcc ttcgaanaca acgtccgcct ggaaatggag cggaaccacg aacgctacgc ttttctgcgc tgggggcagc aggccttcag ccgcttcagc gtggtgccgc cgggcaccgg tatttgccac caggtgaacc tggaatacct tggccgggcg gtctggagcg aggangtgaa cggncagtgg atggcgtggc cggatacgct ggtgggcacc gactcccata ccaccatgat taatggtctt ggcgtgctgg gctggggcgt gggcgggatt gaagcggaan ccgctatgct gggccagccg gtgtcaatgc tgatccccga tgttgtgggc tttaagctca gcggcnaact gcgcgaanna tcaccgccac tgacctggta ctgangntac ccaatgctgc gtcagcatgg cgtcgtcgca gttngttgan tctacgngan gnctgntncn tacccttgcg anaggcgaca tcgccaatnt ggnnncnnnn nnggnncncn gnngcttttc cnancgatga nnnaacgcnc nnctncnnnn nnntcnnnnn nnnnnnaang gtcnnctgnn nannc

Expected Protein Sequence

MAHHHHHHMS STLREASKDT LQVNDKTWHY YSLPLAEKQL GEISRLPKSL KVLMENLLRW QDGDSVTEED IRALAGWLQQ AHADREIAYR PARVLMQDFT GVPAVVDLAA MREAVKRLGG DTAKVNPLSP VDLVIDHSVT VDRFGDDEAF EDNVRLEMER NHERYAFLRW GQQAFSRFSV VPPGTGICHQ VNLEYLGRAV WSEEVNGQWM AWPDTLVGTD SHTTMINGLG VLGWGVGGIE AEAAMLGQPV SMLIPDVVGF KLSGKLREGI TATDLVLTVT QMLRQHGVVG KFVEFYGDGL DTLPLADRAT IANMAPEYGA TCGFFPIDDV TLSYMRLSGR SEEQVALVEA YAKAQGMWRQ PGDEPVFTST LALDMSSVEA SLAGPKRPQD RVALGDVPKA FAASGELEVN HLQRQRQPVD YTLNGHHYSL PDGAVAIAAI TSCTNTSNPS VLMAAGLLAK KAVERGLQPQ PWVKASLAPG SKVVSDYLAH AGLTPYLDQL GFNLVGYGCT TCIGNSGPLP EPIEEAIKKG DLTVGAVLSG NRNFEGRIHP LVKTNWLASP PLVVAYALAG NMNIDLTREP LGQGKNGEPV YLKDIWPSGE EIARAVEQVS TEMFRKEYAE VFSGTEEWKA IKVEASDTYD WQEDSTYIRL SPFFDEMGVE PLPVEDIRGA RILAMLGDSV TTDHISPAGS IKADSPAGRY LQEHGVARRD FNSYGSRRGN HEVMMRGTFA NIRIRNEMVP GVEGGMTRHL PDPEPMAIYD AAMLYKAEGT PLAVIAGKEY GSGSSRDWAA KGPRLLGIRV VIAESFERIH RSNLIGMGIL PLEFPQGVTR KTLRLTGEER IDISNLQSLQ PGATVPVTLT RADGSQEAIP CRCRIDTATE LTYYRNDGIL HYVIRNML

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt caaccctacg agaagccagt aaggacacgc tgcaggtcaa cgataaaacc tggcattact acagtctgcc gttggcggag aagcaactgg gcgagatcag tcgactgccc aagtcgctga aggtgctgat ggagaacctg ctgcgctggc aggacggtga ctcggtaact gaagaagata ttcgcgccct ggccggctgg ctgcaacagg cccatgccga ccgggaaatc gcctaccgtc cggcacgcgt cctgatgcag gactttaccg gcgtgccggc ggtagtggat ctggccgcca tgcgcgaggc ggtgaaacgc cttggcggcg atacggcgaa ggttaatccg ctatcgccgg ttgacctggt gatcgaccac tcggtgaccg ttgaccgctt cggcgacgac gaggccttcg aagacaacgt ccgcctggaa atggagcgga accacgaacg ctacgctttt ctgcgctggg ggcagcaggc cttcagccgc ttcagcgtgg tgccgccggg caccggtatt tgccaccagg tgaacctgga ataccttggc cgggcggtct ggagcgagga ggtgaacggc cagtggatgg cgtggccgga tacgctggtg ggcaccgact cccataccac catgattaat ggtcttggcg tgctgggctg gggcgtgggc gggattgaag cggaagccgc tatgctgggc cagccggtgt caatgctgat ccccgatgtt gtgggcttta agctcagcgg caaactgcgc gaaggcatca ccgccactga cctggtactg acggttaccc aaatgctgcg tcagcatggc gtcgtcggca agtttgttga attctacggt gacggcctgg ataccttacc ccttgccgac agggcgacca tcgccaatat ggcgccggag tatggcgcca cctgtggctt tttccctatc gatgacgtaa cgctcagcta tatgcgtctc agcggccgta gcgaagaaca ggtcgcgctg gtggaagcct acgctaaagc ccagggcatg tggcgccagc ctggcgatga gccggtcttt accagtaccc tggcgctgga tatgagcagc gtcgaagcga gcctcgccgg gccgaagcga ccgcaggatc gcgtggcgct gggcgatgtt ccgaaagcct tcgccgccag cggcgagctg gaggttaacc accttcagcg tcagcgtcag ccggttgact atacgctgaa cgggcatcac tattcgctac cggatggcgc ggtggctatt gcagcgatta cctcctgcac caatacctct aaccccagcg tgttgatggc cgccggcctg ctggcgaaaa aagccgtcga acgcggcctg cagccgcagc cgtgggtgaa agcttctctg gccccaggct cgaaagtggt gtcggattat ctggcccatg ccggcctgac gccttacctc gaccagctcg gcttcaatct ggtgggatat ggttgcacca cctgtattgg taactccggt ccattgcctg aacctatcga agaagcgatc aaaaaaggcg atctgaccgt cggggcagtg ctctccggta accgtaactt cgaagggcgt attcatccgc tggtgaaaac caactggctg gcctcgccgc cgttggtggt cgcctatgcg ctggcgggga acatgaatat cgacctcacc cgcgagccgt tgggacaggg caagaatggc gagccggtct atctgaaaga tatctggccg agcggggagg agattgcccg cgcggtggaa caggtctcca ccgagatgtt ccgcaaagag tacgcggaag tgttttccgg cactgaggag tggaaggcga tcaaggtgga ggcgtcagat acctacgact ggcaggagga ctcgacctat attcgcttgt cgccgttctt tgacgagatg ggcgtcgagc cgctgccggt agaggatatt cgcggcgcgc ggatcctcgc catgctgggc gactcagtca ccacggacca catttcgccg gcggggagca tcaaagccga tagtccggca ggccgctatc tgcaggagca tggggttgcc cggcgcgact ttaactccta tggttcccgt cgcggtaacc atgaggtgat gatgcgtggc accttcgcca atatccgcat ccgtaacgaa atggtcccag gggtggaagg gggtatgacc cgccatctgc cggatcctga accgatggcg atttacgatg cggcgatgct gtacaaggct gagggcacac cgctggcggt gattgccggt aaggagtatg gctcaggttc cagccgtgac tgggcggcga aggggccgcg cctgctgggg attcgggtag tgatcgccga gtccttcgag cgtatccatc gttccaacct gatcggaatg ggcattttgc cgctggagtt cccgcagggc gtcacccgca aaacgctacg gttgaccggg gaagagcgga ttgatatcag taatctgcag tcgcttcagc ctggcgctac ggtgccggtg accctgactc gcgctgacgg ctcgcaggaa gcgatccctt gtcggtgccg tatcgacacg gcgacagaac tcacctacta ccgcaacgac ggcatcctgc actacgttat tcgtaatatg ctgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat