KlpnC.17896.a
Bifunctional ligase/repressor BirA
| CENTER ID: | KlpnC.17896.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.17896.a.B1.GE44330 | Full length(KlpnC.17896.a) | 1 | 320 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
8F8U
DEPOSITED: 11/22/2022
DETERMINATION: XRay
CLONE: KlpnC.17896.a.B1.GE44330
PROTEIN: KlpnC.17896.a.B1.PW39036
8FI3
DEPOSITED: 12/15/2022
DETERMINATION: XRay
CLONE: KlpnC.17896.a.B1.GE44330
PROTEIN: KlpnC.17896.a.B1.PW39036
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|1125630.4.peg.189 |
| UniProt: | A0A0H3GGM9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKDHTIPLTL ISILADGEFH SGEQLGEQLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL PEPIHLLDEK KISQEIDHGR VTVLPVIDST NQYLLDRLDE LTSGDACVAE YQQAGRGRRG RKWFSPFGAN LYLSMYWRLE QGPAAAIGLS LVIGIVIAEV LQQLGAEQVR VKWPNDIYLQ DRKLSGILVE LTGKTGDAAQ IVSGAGINLV MRRVESDVVN QGWISLQEAG VVIDRNLLAA RLIKELRLGL ELFEQEGLAP YLPRWEKLDN FIHRPVKLII GDKEIYGISR GIDAQGALLL EQDGVIKAWV GGEISLRSAE
NT Sequence
GTGAAAGACC ATACTATCCC TTTAACGCTA ATTTCTATCC TTGCTGATGG TGAGTTTCAC TCTGGCGAAC AGCTTGGTGA GCAGTTAGGC ATGAGCCGGG CGGCGATTAA TAAGCACATC CAGACCTTAC GTGACTGGGG CGTCGATGTG TTTACTGTGC CGGGCAAGGG TTACAGCCTG CCGGAGCCTA TTCATCTATT AGATGAGAAA AAGATTTCTC AAGAGATAGA CCATGGCCGA GTGACAGTCC TTCCCGTCAT CGACTCTACT AACCAGTATT TGTTGGACAG GCTTGATGAG CTGACGTCCG GAGATGCCTG TGTGGCTGAA TATCAGCAAG CAGGGCGAGG CCGGCGAGGC AGAAAATGGT TTTCGCCCTT TGGCGCGAAT CTCTATCTCT CCATGTACTG GCGTCTGGAG CAGGGCCCGG CAGCGGCTAT TGGCCTAAGC CTGGTGATCG GTATTGTGAT CGCTGAAGTT CTGCAGCAGC TCGGGGCCGA ACAGGTTCGC GTGAAATGGC CAAACGATAT TTACCTGCAG GATCGTAAGC TGTCAGGGAT CCTCGTAGAA CTGACGGGGA AAACGGGCGA TGCCGCACAA ATCGTCAGCG GCGCGGGTAT CAACCTTGTG ATGCGTCGCG TGGAGTCTGA TGTCGTCAAT CAGGGGTGGA TCAGCCTTCA GGAAGCGGGG GTGGTCATCG ACCGTAACCT CCTCGCCGCG CGCCTGATTA AAGAGCTGCG CCTGGGACTG GAGCTGTTCG AGCAGGAAGG ATTGGCTCCC TATCTACCGC GCTGGGAAAA ACTGGATAAC TTTATTCATC GCCCGGTGAA ACTGATTATC GGTGATAAAG AAATTTACGG CATTTCGCGT GGCATTGACG CCCAGGGCGC ACTATTGCTG GAGCAGGATG GTGTAATAAA AGCTTGGGTG GGTGGTGAAA TATCATTGCG CAGTGCAGAA
Details for KlpnC.17896.a.B1.GE44330
| HARVESTED ON: | 5/21/2021 |
| SEQUENCED ON: | 6/4/2021 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 92 |
| PERCENT COVERAGE: | 86 |
Validated AA Sequence
MAHHHHHHMV KDHTIPLTLI SILADGEFHS GEQLGEQLGM SRAAINKHIQ TLRDWGVDVF TVPGKGYSLP EPIHLLDEKK ISQEIDHGRV TVLPVIDSTN QYLLDRLDEL TSGDACVAEY QQAGRGRRGR KWFSPFGANL YLSMYWRLEQ GPAAAIGLSL VIGIVIAEVL XQLGAEQVRV KWPNDIYLQD RKLSGILVEL TGKTGDAAQI VSGAGIXLVM RRVESDVVXX GWISLXXAGV VXDRNLLAAR LIKELRLGLE LFXQXXGSLS TALGKXGYFI HRR
Validated NT Sequence
tacgactcnc tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt annaaggaga tataccatgg ctcaccacca ccaccaccat atggtgaaag accatactat ccctttaacg ctaatttcta tccttgctga tggtgagttt cactctggcg aacagcttgg tgagcagtta ggcatgagcc gggcggcgat taataagcac atccagacct tacgtgactg gggcgtcgat gtgtttactg tgccgggcaa gggttacagc ctgccggagc ctattcatct attagatgag aaaaagattt ctcaagagat agaccatggc cgagtgacgg tccttcccgt catcgactct actaaccagt atttgctgga caggcttgat gagctgacgt ccggagatgc ctgtgtggct gaatatcagc aagcagggcg aggccggcga ggcagaaaat ggttttcgcc ctttggcgcg aatctctatc tctcgatgta ctggcgtctg gagcagggcc cggcagcggc tattggtcta agcctggtga tcggtattgt gatcgctgaa gttctgcanc agctcggggc cgaacaggtt cgcgtgaaat ggccaaacga tatttacctg caggatcgta agctgtcagg gatcctcgtg gaactgacgg ggaaaacggg cgatgccgca caaatcgtca gcggcgcggg tatcnacctt gtgatgcgtc gcgtggagtc tgatgtcgtc natcangggt ggatcagcct tcnnnaagcg ggggtggtcn tcgaccgnaa cctcctcgcc gcgcgcctga ttaaagagct gcgcctggga ctggagctgt tcnagcagnn gantggctcc ctatctaccg cgctgggaaa anntggatac tttattcatc gccggtgaaa ctgatntcgg tganaagaan tttacnggna tttcccgtgn nattganncc cnggcgcact atngctggag cnnatggngn nnntaaaanc ctggnnggnn gnnaannnca tngcncanng cnnaataang agtanatang gatccgcngc taacaanccc aanggagnnn nanttnncnn nntnncnnc
Expected Protein Sequence
MAHHHHHHMK DHTIPLTLIS ILADGEFHSG EQLGEQLGMS RAAINKHIQT LRDWGVDVFT VPGKGYSLPE PIHLLDEKKI SQEIDHGRVT VLPVIDSTNQ YLLDRLDELT SGDACVAEYQ QAGRGRRGRK WFSPFGANLY LSMYWRLEQG PAAAIGLSLV IGIVIAEVLQ QLGAEQVRVK WPNDIYLQDR KLSGILVELT GKTGDAAQIV SGAGINLVMR RVESDVVNQG WISLQEAGVV IDRNLLAARL IKELRLGLEL FEQEGLAPYL PRWEKLDNFI HRPVKLIIGD KEIYGISRGI DAQGALLLEQ DGVIKAWVGG EISLRSAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaag accatactat ccctttaacg ctaatttcta tccttgctga tggtgagttt cactctggcg aacagcttgg tgagcagtta ggcatgagcc gggcggcgat taataagcac atccagacct tacgtgactg gggcgtcgat gtgtttactg tgccgggcaa gggttacagc ctgccggagc ctattcatct attagatgag aaaaagattt ctcaagagat agaccatggc cgagtgacag tccttcccgt catcgactct actaaccagt atttgttgga caggcttgat gagctgacgt ccggagatgc ctgtgtggct gaatatcagc aagcagggcg aggccggcga ggcagaaaat ggttttcgcc ctttggcgcg aatctctatc tctccatgta ctggcgtctg gagcagggcc cggcagcggc tattggccta agcctggtga tcggtattgt gatcgctgaa gttctgcagc agctcggggc cgaacaggtt cgcgtgaaat ggccaaacga tatttacctg caggatcgta agctgtcagg gatcctcgta gaactgacgg ggaaaacggg cgatgccgca caaatcgtca gcggcgcggg tatcaacctt gtgatgcgtc gcgtggagtc tgatgtcgtc aatcaggggt ggatcagcct tcaggaagcg ggggtggtca tcgaccgtaa cctcctcgcc gcgcgcctga ttaaagagct gcgcctggga ctggagctgt tcgagcagga aggattggct ccctatctac cgcgctggga aaaactggat aactttattc atcgcccggt gaaactgatt atcggtgata aagaaattta cggcatttcg cgtggcattg acgcccaggg cgcactattg ctggagcagg atggtgtaat aaaagcttgg gtgggtggtg aaatatcatt gcgcagtgca gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat