SSGCID: Target Page

KlpnC.17683.a

3-dehydroquinate synthase

CENTER ID:	KlpnC.17683.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.17683.a.B1.GE44326	Full length(KlpnC.17683.a)		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.4798
UniProt:	A0A0H3GU70

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLVTNETLAP LYLDTVRSAL EQSGVNVDSV ILPDGEQYKS LAVMDTVFTA LLQKPHGRDT TLVALGGGVI GDLTGFAAAS YQRGVRFIQV PTTLLSQVDS SVGGKTAVNH PLGKNMIGAF WQPVSVVVDL NCLKTLPKRE LASGLAEVIK YGVILDGEFF SWLENNIDAL LALDDTAMAY CIRRCCELKA EVVAADERET GLRALLNLGH TFGHAIEAEM GYGNWLHGEA VAAGMVMAAR TSERLGQFRA QDTQRIIDLL KRAGLPVRGP QEMSAQAYLP HMMRDKKVLA GEMRLVLPLA IGSSELRGGV PHDVVLGAIA DTQQAQQ

NT Sequence

ATGCTGGTCA CCAACGAAAC GCTGGCGCCG CTTTATCTGG ATACCGTCCG CTCTGCGCTG GAGCAGTCAG GGGTCAACGT TGACAGCGTC ATTCTGCCAG ACGGCGAGCA ATACAAAAGC CTGGCGGTGA TGGATACCGT GTTTACTGCG CTGTTGCAAA AACCACATGG CCGCGACACC ACTCTGGTGG CGCTTGGCGG GGGGGTGATC GGTGATTTAA CCGGTTTCGC CGCGGCAAGC TATCAGCGCG GCGTGCGTTT CATCCAGGTC CCTACCACCC TGTTGTCGCA GGTCGACTCG TCGGTCGGCG GCAAAACCGC AGTGAACCAC CCTCTCGGCA AAAACATGAT TGGCGCCTTC TGGCAGCCGG TTTCCGTGGT AGTTGATCTT AACTGTCTGA AAACTCTGCC GAAGCGCGAG CTCGCTTCCG GACTGGCTGA GGTCATCAAG TACGGGGTGA TCCTGGATGG TGAATTCTTC AGCTGGCTGG AAAACAACAT TGATGCGCTG CTGGCGCTGG ACGATACCGC CATGGCGTAC TGCATTCGCC GCTGCTGTGA ACTGAAAGCG GAGGTTGTGG CAGCCGACGA GCGTGAAACC GGGTTACGTG CTTTACTCAA TCTTGGACAT ACCTTCGGCC ATGCTATCGA AGCGGAAATG GGCTACGGTA ACTGGCTGCA TGGCGAAGCG GTGGCGGCAG GTATGGTGAT GGCGGCACGC ACGTCCGAAC GGCTGGGGCA GTTCCGCGCG CAGGATACTC AGCGCATTAT CGATCTGCTC AAGCGTGCAG GCTTACCGGT ACGCGGCCCG CAGGAAATGA GTGCGCAGGC GTATTTGCCC CATATGATGC GCGATAAAAA GGTACTGGCT GGCGAAATGC GGCTGGTGCT CCCGCTCGCA ATAGGAAGCA GCGAGCTGCG CGGCGGAGTG CCGCACGATG TGGTTCTTGG CGCGATTGCT GATACTCAGC AGGCGCAACA A

Details for KlpnC.17683.a.B1.GE44326

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	83

Validated AA Sequence

MAHHHHHHML VTNETLAPLY LDTVRSVLEQ SGVNVDSVIL PDGEQYKSLA VMDTVFTALL QKPHGRDTTL VALGGGVIGD LTGFAAASYQ RGVRFIQVPT TLLSQVDSSV GGKTAVNHPL GKNMIGAFWQ PVSVVVDLNC LKTLPKRELA SGLAEVIKYG VILDGEFFSW LENNIDALLA LDDTAMAYCI RRCCELKAEV VAADERETGL RALLNLGHTF GHAIEAEMGY GNWLHGEAVA AXMVMAARTS ERLGQFRAXD TQRIIDLLKR AGLPVRGPQK

Validated NT Sequence

acgactcnct atagggagac nnnancggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tgctggtcac caacgaaacg ctggcgccgc tttatctgga taccgtccgc tctgtgctgg agcagtcagg ggtcaacgtt gacagcgtca ttctgccaga cggcgagcaa tacaaaagcc tggcggtgat ggataccgtg tttactgcgc tgttgcaaaa accacatggc cgcgacacca ctctggtggc gcttggcggg ggggtgatcg gtgatttaac cggtttcgcc gcggcaagct atcagcgcgg cgtgcgtttc atccaggtcc ctaccaccct gttgtcgcag gtcgactcgt cggtcggcgg caaaaccgca gtgaaccacc ctctcggcaa aaacatgatt ggcgccttct ggcagccggt ttccgtggta gttgatctta actgtctgaa aactctgccg aagcgcgagc tcgcttccgg actggctgag gtcatcaagt acggggtgat cctggatggt gaattcttca gctggctgga aaacaacatt gatgcgctgc tggcgctgga cgataccgcc atggcgtact gcattcgccg ctgctgtgaa ctgaaagcgg aggttgtggc agccgacgag cgtgaaaccg ggttacgtgc tttactcaat cttggacata ccttcggcca tgctatcgaa gcggaaatgg gctacggtaa ctggctgcat ggcgaagcgg tggcggcang tatggtgatg gcggcacgca cgtccgaacg gctggggcag ttccgcgcgc angatactca gcgcattatc gatctgctca agcgtgcagg cttaccggta cgcggcccgc agaaatgant gcgcnagcgt antttgcccc atatgatgcg cnanaaaagt actggntggc gaaatgcggc tggtgnttcc cgntcgcaaa naggaagcan cgagctgcgc ggcggantgc cncangnngg tngtttctnn gngc

Expected Protein Sequence

MAHHHHHHML VTNETLAPLY LDTVRSALEQ SGVNVDSVIL PDGEQYKSLA VMDTVFTALL QKPHGRDTTL VALGGGVIGD LTGFAAASYQ RGVRFIQVPT TLLSQVDSSV GGKTAVNHPL GKNMIGAFWQ PVSVVVDLNC LKTLPKRELA SGLAEVIKYG VILDGEFFSW LENNIDALLA LDDTAMAYCI RRCCELKAEV VAADERETGL RALLNLGHTF GHAIEAEMGY GNWLHGEAVA AGMVMAARTS ERLGQFRAQD TQRIIDLLKR AGLPVRGPQE MSAQAYLPHM MRDKKVLAGE MRLVLPLAIG SSELRGGVPH DVVLGAIADT QQAQQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgg tcaccaacga aacgctggcg ccgctttatc tggataccgt ccgctctgcg ctggagcagt caggggtcaa cgttgacagc gtcattctgc cagacggcga gcaatacaaa agcctggcgg tgatggatac cgtgtttact gcgctgttgc aaaaaccaca tggccgcgac accactctgg tggcgcttgg cgggggggtg atcggtgatt taaccggttt cgccgcggca agctatcagc gcggcgtgcg tttcatccag gtccctacca ccctgttgtc gcaggtcgac tcgtcggtcg gcggcaaaac cgcagtgaac caccctctcg gcaaaaacat gattggcgcc ttctggcagc cggtttccgt ggtagttgat cttaactgtc tgaaaactct gccgaagcgc gagctcgctt ccggactggc tgaggtcatc aagtacgggg tgatcctgga tggtgaattc ttcagctggc tggaaaacaa cattgatgcg ctgctggcgc tggacgatac cgccatggcg tactgcattc gccgctgctg tgaactgaaa gcggaggttg tggcagccga cgagcgtgaa accgggttac gtgctttact caatcttgga cataccttcg gccatgctat cgaagcggaa atgggctacg gtaactggct gcatggcgaa gcggtggcgg caggtatggt gatggcggca cgcacgtccg aacggctggg gcagttccgc gcgcaggata ctcagcgcat tatcgatctg ctcaagcgtg caggcttacc ggtacgcggc ccgcaggaaa tgagtgcgca ggcgtatttg ccccatatga tgcgcgataa aaaggtactg gctggcgaaa tgcggctggt gctcccgctc gcaataggaa gcagcgagct gcgcggcgga gtgccgcacg atgtggttct tggcgcgatt gctgatactc agcaggcgca acaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat