KlpnC.17669.a

dTDP-4-dehydrorhamnose reductase

CENTER ID: KlpnC.17669.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.17669.a.B1.GE44325 Full length(KlpnC.17669.a) 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Structures
8CTR
DEPOSITED: 5/16/2022
DETERMINATION: XRay
CLONE: KlpnC.17669.a.B1.GE44325
PROTEIN: KlpnC.17669.a.B1.PW39034
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|1125630.4.peg.3464
UniProt: A0A0H3H0K8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKILLIGKNG QVGWELQRSL STLGDVVAVD YFDKELCGDL TNLDGIAQTV RTVRPDVVVN AAAHTAVDKA ESERELSDLL NDKGVAVLAA ESAKLGALMV HYSTDYVFDG AGSHYRREDE ATGPLNVYGE TKRAGELALE QGNPRHLIFR TSWVYATRGA NFAKTMLRLA GEKETLSIID DQHGAPTGAE LLADCTATAI RETLRDPALA GTYHLVASGE TSWCDYARYV FEVARAHGAE LAVQEVKGIP TTAYPTPAKR PLNSRLSNEK FQQAFGVTLP DWRQGVARVV TEVLGK
NT Sequence
ATGAAGATAT TACTGATTGG CAAGAATGGT CAGGTGGGCT GGGAGCTGCA GCGCTCCCTG TCCACGCTCG GTGATGTGGT CGCGGTGGAT TACTTTGATA AAGAGCTGTG CGGTGATCTG ACTAATCTGG ACGGTATCGC GCAGACCGTG CGTACCGTCC GCCCGGACGT GGTGGTGAAT GCCGCCGCGC ACACGGCAGT GGACAAGGCC GAAAGCGAGC GGGAGCTCTC CGACCTGCTG AACGACAAAG GGGTGGCGGT GCTGGCGGCG GAGTCGGCGA AGCTGGGTGC CCTGATGGTG CACTATTCCA CGGACTATGT GTTCGACGGC GCCGGCAGCC ATTATCGCCG GGAAGATGAG GCAACCGGCC CGCTGAACGT CTACGGTGAA ACCAAACGTG CCGGTGAACT GGCGCTGGAG CAGGGCAACC CGCGTCACCT GATTTTCCGT ACCAGCTGGG TGTATGCCAC CCGCGGCGCC AACTTTGCCA AAACCATGCT CCGCCTGGCG GGTGAGAAAG AGACGCTCTC CATCATTGAC GACCAGCACG GGGCGCCCAC CGGAGCAGAG CTGCTGGCAG ACTGCACGGC GACGGCAATC CGTGAAACGC TGCGTGATCC GGCGCTGGCC GGTACCTATC ATCTGGTGGC CAGCGGCGAG ACCAGCTGGT GCGACTACGC CCGCTATGTG TTTGAGGTGG CGAGAGCGCA CGGTGCAGAG CTGGCGGTTC AGGAAGTGAA GGGCATTCCG ACGACGGCCT ATCCGACGCC GGCGAAGCGT CCGCTCAACT CTCGCCTGTC GAATGAAAAA TTCCAGCAGG CATTTGGGGT GACTCTCCCG GACTGGCGTC AGGGTGTGGC TCGCGTGGTA ACAGAAGTCC TGGGTAAA
Details for KlpnC.17669.a.B1.GE44325
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK ILLIGKNGQV GWELQRSLST LGDVVAVDYF DKELCGDLTN LDGIAQTVRT VRPDVVVNAA AHTAVDKAES ERELSDLLND KGVAVLAAES AKLGALMVHY STDYVFDGAG SHYRREDEAT GPLNVYGETK RAGELALEQG NPRHLIFRTS WVYATRGANF AKTMLRLAGE KETLSIIDDQ HGAPTGAELL ADCTATAIRE TLRDPALAGT YHLVASGETS WCDYARYVFE VARAHGAELA VQEVKGIPTT AYPTPAKRPL NSRLSNEKFQ QAFGVTLPDW RQGVARVVTE VLGK
Validated NT Sequence
cggatcctat cttactcnct tatttaccca ggacttctgt taccacgcga gccacaccct gacgccagtc cgggagagtc accccgaatg cctgctggaa tttttcattc gacaggcgag agttgagcgg acgcttcgcc ggcgtcggat aggccgtcgt cggaatgccc ttcacttcct gaaccgccag ctcggcaccg tgcgctctgg ccacctcaaa cacatagcgg gcgtagtcgc accagctggt ctcgccgctg gccaccagat gataggtgcc ggccagcgcc ggatcacgca gcgtttcacg gattgccgtc gccgtgcagt ctgccagcag ctctgcgccg gtgggcgccc cgtgctggtc gtcaatgatg gagagcgtct ctttctcacc cgccaggcgg agcatggttt tggcaaagtt ggcaccgcgg gtggcataca cccagctggt acggaaaatc aggtgacgcg ggttgccctg ctccagcgcc agttcaccgg cacgcttggt ttcgccgtag acgttcagcg ggccggttgc ctcatcttcc cggcgataat gactaccggc gccgtcgaac acatagtccg tggaatagtg caccatcagg gcacccagct tcgccgactc cgccgccagc accgccaccc ctttgtcgtt cagcaggtcg gagagctccc gctcgctttc ggccttgtca actgccgtgt gcgcggcggc attcaccacc acgtccgggc gaacagtacg cacggtctgc gcgataccgt ccagattagt cagatcgccg cacagctctt tatcaaagta atccaccgcg accacatcac cgagcgtgga gagggagcgc tgcagctccc agcccacctg accattcttg ccaatcagta atatcttcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMK ILLIGKNGQV GWELQRSLST LGDVVAVDYF DKELCGDLTN LDGIAQTVRT VRPDVVVNAA AHTAVDKAES ERELSDLLND KGVAVLAAES AKLGALMVHY STDYVFDGAG SHYRREDEAT GPLNVYGETK RAGELALEQG NPRHLIFRTS WVYATRGANF AKTMLRLAGE KETLSIIDDQ HGAPTGAELL ADCTATAIRE TLRDPALAGT YHLVASGETS WCDYARYVFE VARAHGAELA VQEVKGIPTT AYPTPAKRPL NSRLSNEKFQ QAFGVTLPDW RQGVARVVTE VLGK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga tattactgat tggcaagaat ggtcaggtgg gctgggagct gcagcgctcc ctgtccacgc tcggtgatgt ggtcgcggtg gattactttg ataaagagct gtgcggtgat ctgactaatc tggacggtat cgcgcagacc gtgcgtaccg tccgcccgga cgtggtggtg aatgccgccg cgcacacggc agtggacaag gccgaaagcg agcgggagct ctccgacctg ctgaacgaca aaggggtggc ggtgctggcg gcggagtcgg cgaagctggg tgccctgatg gtgcactatt ccacggacta tgtgttcgac ggcgccggca gccattatcg ccgggaagat gaggcaaccg gcccgctgaa cgtctacggt gaaaccaaac gtgccggtga actggcgctg gagcagggca acccgcgtca cctgattttc cgtaccagct gggtgtatgc cacccgcggc gccaactttg ccaaaaccat gctccgcctg gcgggtgaga aagagacgct ctccatcatt gacgaccagc acggggcgcc caccggagca gagctgctgg cagactgcac ggcgacggca atccgtgaaa cgctgcgtga tccggcgctg gccggtacct atcatctggt ggccagcggc gagaccagct ggtgcgacta cgcccgctat gtgtttgagg tggcgagagc gcacggtgca gagctggcgg ttcaggaagt gaagggcatt ccgacgacgg cctatccgac gccggcgaag cgtccgctca actctcgcct gtcgaatgaa aaattccagc aggcatttgg ggtgactctc ccggactggc gtcagggtgt ggctcgcgtg gtaacagaag tcctgggtaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat