KlpnC.17519.a

ADP-heptose:LPS heptosyltransferase II

CENTER ID: KlpnC.17519.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.17519.a.B1.GE44322 Full length(KlpnC.17519.a) 1 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|1125630.4.peg.5008
UniProt: A0A0H3H038

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGDMMMSQSL YRTLRARYPQ AIIDVMAPAW CRPLLSRMPE VNEAIPMPLG HGALAIGERR KLGHSLRERR YDRAYVLPNS FKSALVPFFA NIPLRTGWRG EMRYGLLNDA RVLDKDAWPL MVERYVALAY DNGVMRCAKD LPQPLLWPQL QVNEGEKSQA CSAFNLSNDR PIVGFCPGAE FGPAKRWPHY HYAALAKKLI DDGYQIALFG SAKDNEAGKE IIAALSSEQQ AWCRNLAGET QLEQAVILIA ACKAVVTNDS GLMHVAAALD RPLVALYGPS SPDFTPPLSH KARVIRLITG YHKVRKGDAA EGYHQSLIDI TPERVLQELN ELLAEKTEHE EA
NT Sequence
GTGGGCGACA TGATGATGTC GCAAAGTCTT TATCGTACGC TCAGGGCGCG CTATCCCCAG GCGATTATCG ACGTGATGGC GCCAGCCTGG TGCCGTCCGT TGCTTTCGCG CATGCCGGAA GTGAACGAAG CGATCCCGAT GCCGCTGGGT CACGGCGCGC TGGCTATCGG CGAACGCCGC AAGCTCGGCC ACAGCCTGCG CGAGCGTCGC TACGATCGCG CTTACGTGCT GCCGAACTCG TTTAAATCGG CATTAGTCCC TTTCTTTGCC AATATCCCGC TGCGCACCGG CTGGCGCGGC GAGATGCGCT ACGGCCTGCT CAACGACGCC CGAGTGCTGG ACAAAGACGC CTGGCCGCTG ATGGTGGAAC GCTACGTGGC GCTGGCCTAC GACAACGGCG TGATGCGCTG CGCCAAAGAT CTGCCGCAGC CGCTGCTGTG GCCGCAGCTG CAGGTCAACG AAGGGGAAAA GTCGCAGGCC TGCAGCGCCT TTAACCTGTC CAACGATCGG CCGATCGTCG GCTTCTGCCC CGGCGCGGAG TTCGGCCCGG CCAAACGCTG GCCGCACTAT CATTATGCTG CGCTGGCGAA AAAGCTTATC GACGATGGCT ATCAGATTGC GCTTTTCGGC TCGGCGAAAG ATAACGAGGC CGGCAAAGAG ATCATCGCCG CCCTCAGCAG CGAGCAGCAG GCCTGGTGCC GTAATCTGGC GGGTGAAACC CAGCTGGAGC AGGCGGTCAT TCTGATCGCC GCCTGTAAAG CTGTAGTGAC TAACGATTCG GGGCTGATGC ACGTCGCCGC AGCGCTGGAC CGCCCGCTGG TGGCGCTGTA TGGCCCGAGC AGTCCGGACT TTACCCCGCC GCTGTCGCAT AAAGCGCGGG TTATCCGTCT GATTACCGGC TACCACAAGG TGCGCAAAGG CGATGCCGCC GAAGGCTATC ATCAGAGCCT GATCGACATC ACCCCAGAAC GCGTCCTGCA AGAGCTGAAC GAACTGTTAG CGGAAAAAAC CGAGCACGAG GAAGCG
Details for KlpnC.17519.a.B1.GE44322
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 63
Validated AA Sequence
MAHHHHHHMV GDMMMSQSLY RTLRARYPQA IIDVMAPAWC RPLLSRMPEV NEAIPMPLGH GALAIGERRK LGHSLRERRY DRAYVLPNSF KSALVPFFAN IPLRTGWRGE MRYGLLNDAR VLDKDAWPLM VERYVALAYD NGVMRCAKDL PQPLLWPQLQ VNEGEKSQAC XAFNLSNDRP IVGFCPGAEF GPAKRWPHYH YAALAKKAYR XWLSDCAFRL GER
Validated NT Sequence
gnnnnnnnnn nannnnacga ctcnctatag ggagaccaca acggtttccc tctagaaata attttgttta actttangaa ggagatatac catggctcac caccaccacc accatatggt gggcgacatg atgatgtcgc aaagtcttta tcgtacgctc agggcgcgct atccccaggc gattatcgac gtgatggcgc cagcctggtg ccgtccgttg ctttcgcgca tgccggaagt gaacgaagcg atcccgatgc cgctgggtca cggcgcgctg gctatcggcg aacgccgtaa gctcggccac agcctgcgcg aacgtcgcta cgatcgcgct tacgtgctgc cgaactcgtt taaatcggca ttagtccctt tctttgccaa tatcccgctg cgcaccggct ggcgcggcga gatgcgctac ggcctgctca acgacgcccg ggtgctggac aaagacgcct ggccgctgat ggtggaacgc tacgtggcgc tggcctacga caacggcgtg atgcgctgcg ccaaagatct gccgcagccg ctgctgtggc cgcagctgca ggtcaacgaa ggggaaaagt cgcaggcctg cancgccttt aacctgtcca acgatcggcc gatcgtcggc ttctgccccg gcgcggagtt cggcccggcc aaacgctggc cgcactatca ctatgctgcg ctggcgaaaa aagcttatcg acnatggcta tcagattgcg cttttcggct cggcgaaaga taacgaggnc ggcnaagana tcatcgccgc cctcagcagc gagcnncnnn ctggtgccgt aatctggcgg gtgaaaccca gctggagcag gcggncattc tgatcgccgc ctgtaaagct gtagtgacta acgattcngg gctgatgcac gtcgccgcag cgctggancg cccgctggtg gcgctngnat ggcccgagca gtccngannt ttaccccgcc gctgtcgcat aaagcgcggg tnattcgtct gantacnggc tannncangg tgcnnnnaag gcgatgcncn naggntnntc atcnnagcct gatcgacatc nncccagaan nnnnncctgc anannnnnga angaannatt annngaaaaa nc
Expected Protein Sequence
MAHHHHHHMG DMMMSQSLYR TLRARYPQAI IDVMAPAWCR PLLSRMPEVN EAIPMPLGHG ALAIGERRKL GHSLRERRYD RAYVLPNSFK SALVPFFANI PLRTGWRGEM RYGLLNDARV LDKDAWPLMV ERYVALAYDN GVMRCAKDLP QPLLWPQLQV NEGEKSQACS AFNLSNDRPI VGFCPGAEFG PAKRWPHYHY AALAKKLIDD GYQIALFGSA KDNEAGKEII AALSSEQQAW CRNLAGETQL EQAVILIAAC KAVVTNDSGL MHVAAALDRP LVALYGPSSP DFTPPLSHKA RVIRLITGYH KVRKGDAAEG YHQSLIDITP ERVLQELNEL LAEKTEHEEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgggcg acatgatgat gtcgcaaagt ctttatcgta cgctcagggc gcgctatccc caggcgatta tcgacgtgat ggcgccagcc tggtgccgtc cgttgctttc gcgcatgccg gaagtgaacg aagcgatccc gatgccgctg ggtcacggcg cgctggctat cggcgaacgc cgcaagctcg gccacagcct gcgcgagcgt cgctacgatc gcgcttacgt gctgccgaac tcgtttaaat cggcattagt ccctttcttt gccaatatcc cgctgcgcac cggctggcgc ggcgagatgc gctacggcct gctcaacgac gcccgagtgc tggacaaaga cgcctggccg ctgatggtgg aacgctacgt ggcgctggcc tacgacaacg gcgtgatgcg ctgcgccaaa gatctgccgc agccgctgct gtggccgcag ctgcaggtca acgaagggga aaagtcgcag gcctgcagcg cctttaacct gtccaacgat cggccgatcg tcggcttctg ccccggcgcg gagttcggcc cggccaaacg ctggccgcac tatcattatg ctgcgctggc gaaaaagctt atcgacgatg gctatcagat tgcgcttttc ggctcggcga aagataacga ggccggcaaa gagatcatcg ccgccctcag cagcgagcag caggcctggt gccgtaatct ggcgggtgaa acccagctgg agcaggcggt cattctgatc gccgcctgta aagctgtagt gactaacgat tcggggctga tgcacgtcgc cgcagcgctg gaccgcccgc tggtggcgct gtatggcccg agcagtccgg actttacccc gccgctgtcg cataaagcgc gggttatccg tctgattacc ggctaccaca aggtgcgcaa aggcgatgcc gccgaaggct atcatcagag cctgatcgac atcaccccag aacgcgtcct gcaagagctg aacgaactgt tagcggaaaa aaccgagcac gaggaagcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat