KlpnC.17386.b

High-affinity zinc uptake system protein ZnuA

CENTER ID: KlpnC.17386.b
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.17386.b.B2.GE44318 Mature protein(KlpnC.17386.b) 26 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|1125630.4.peg.3304
UniProt: A0A0H3GVG5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLHKNTLLCA GLGAVFLFAQ VPLASAAVVT SMKPLGFIAA AIADGVTETQ VLLPDGASEH DYSLRPSDVK RLQNADLVVW IGPEMEAFMD KSTQSIAANK KVTIAELDGV KPLLITGADD DDDHHGHGHG AAEKGDGDHH HGIYNMHLWL SPEIARLSAV AIHDKLLELM PQSRAKLDSN LQQFETALAA TDKQVSNELA PLKGKGYFVF HDAYGYFEKH YGLTSLGHFT VNPEIQPGAQ RLHEIRTQLV EQKATCVFAE PQFRPAVIEA VARGTSVRMG TLDPLGTGIT LGKTSYPQFL TQLANQYSSC LKGD
NT Sequence
ATGTTACATA AAAATACGCT TCTTTGCGCT GGACTTGGCG CTGTTTTTTT GTTCGCGCAG GTACCGCTGG CCAGCGCCGC CGTGGTGACT TCTATGAAAC CGCTGGGCTT TATCGCGGCG GCCATCGCCG ATGGGGTGAC CGAGACCCAG GTGCTGCTGC CCGATGGCGC CTCCGAGCAT GATTATTCCC TCCGTCCTTC TGATGTAAAA CGCTTACAGA ACGCAGACTT AGTGGTGTGG ATTGGTCCGG AAATGGAAGC CTTTATGGAT AAGTCGACGC AAAGCATTGC GGCGAATAAA AAGGTGACGA TTGCCGAGCT GGATGGGGTC AAACCGCTGC TCATCACCGG GGCGGACGAC GATGACGATC ACCATGGTCA TGGCCATGGC GCAGCAGAAA AAGGTGACGG CGATCACCAT CACGGTATTT ACAATATGCA TCTGTGGTTA TCCCCAGAGA TAGCGCGGCT TTCGGCGGTT GCAATCCACG ATAAATTATT GGAACTTATG CCGCAGAGTC GAGCCAAACT CGACAGCAAC CTGCAGCAGT TCGAGACCGC GCTGGCGGCG ACCGACAAGC AGGTGAGCAA TGAGCTGGCA CCGCTGAAAG GGAAGGGCTA TTTCGTTTTT CATGACGCCT ACGGTTACTT TGAAAAACAC TACGGTTTGA CCTCGCTGGG GCACTTTACC GTCAACCCTG AAATACAGCC CGGTGCGCAG CGTTTACACG AAATCAGAAC ACAACTGGTT GAGCAGAAAG CCACTTGCGT TTTTGCTGAG CCACAGTTCA GGCCAGCGGT TATCGAAGCT GTTGCCCGGG GCACGTCTGT GCGCATGGGG ACCCTGGATC CACTGGGGAC AGGAATTACG CTGGGTAAAA CGAGTTACCC GCAATTCCTC ACCCAACTGG CGAATCAGTA TTCGAGCTGC CTGAAAGGAG AT
Details for KlpnC.17386.b.B2.GE44318
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 78
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMA AVVTSMKPLG FIAAAIADGV TETQVLLPDG ASEHDYSLRP SDVKRLQNAD LVVWIGPEME AFMDKSTQSI AANKKVTIAV LDGVKPLLIT GADDDDDHHG HDHGAAEKGD GDHHHGIYNM HLWLSPEIAR LSAVAIHDKL LELMPQSRAK LDSNLQQFET ALAATDKQVS NELAPLKGKG YFVFHDAYGY FEKTLRFDLA GALYRXTLKY SPVRSVYTKS EHNWLSRKPL AFLLSHSSXQ RLSKLXARGT SVRMGTLXPL GTGITLGKTX YSQFLTHWRI SIRAA
Validated NT Sequence
tnnnnnngac tcnctatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttannaag gagatatacc atggctcacc accaccacca ccatatggcc gccgtggtga cttctatgaa accgctgggc tttatcgcgg cggccatcgc cgatggggtg accgagaccc aggtgctgct gcccgatggc gcctccgagc atgattattc cctccgtcct tctgatgtaa aacgcttaca gaacgcagac ttagtggtgt ggattggtcc ggaaatggaa gcctttatgg ataagtcgac gcaaagcatt gcggcgaata aaaaggtgac gattgccgtg ctggatgggg tcaaaccgct gctcatcacc ggggcggacg acgatgacga tcaccatggt catgaccatg gcgcagcaga aaaaggtgac ggcgatcacc atcacggtat ttacaatatg catctgtggt tatccccaga gatagcgcgg ctttcggcgg ttgcaatcca cgataaatta ttggaactta tgccgcagag tcgagccaaa ctcgacagca acctgcagca gttcgagacc gcgctggcgg cgaccgacaa gcaggtgagc aatgagctgg caccgctgaa agggaagggc tatttcgttt ttcatgacgc ctacggttac tttgaaaaaa cactacggtt tgacctcgct ggggcacttt accgtcnaac cctgaaatac agcccggtgc gcagcgttta cacgaaatca gaacacaact ggttgagcag aaagccactt gcgtttttgc tgagccacag ttcangccag cggttatcga agctgnttgc ccggggcacg tctgtgcgca tggggaccct gnatccactg gggacaggaa ttacgctggg taaaacgant tactcgcaat tcctcaccca ctggcgaatc agtattcgag ctgcctgaag gagattaagt gagtaagata ggatccggct gctancnaag cccnaaanga agctganttg gctgctnnnc cgntgagcaa tnactagcat accccntggg gnnnctanng ggtagggntt ttgcttnann ggnggaacta nnnccngnnn nnccnnng
Expected Protein Sequence
MAHHHHHHAA VVTSMKPLGF IAAAIADGVT ETQVLLPDGA SEHDYSLRPS DVKRLQNADL VVWIGPEMEA FMDKSTQSIA ANKKVTIAEL DGVKPLLITG ADDDDDHHGH GHGAAEKGDG DHHHGIYNMH LWLSPEIARL SAVAIHDKLL ELMPQSRAKL DSNLQQFETA LAATDKQVSN ELAPLKGKGY FVFHDAYGYF EKHYGLTSLG HFTVNPEIQP GAQRLHEIRT QLVEQKATCV FAEPQFRPAV IEAVARGTSV RMGTLDPLGT GITLGKTSYP QFLTQLANQY SSCLKGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgccg tggtgacttc tatgaaaccg ctgggcttta tcgcggcggc catcgccgat ggggtgaccg agacccaggt gctgctgccc gatggcgcct ccgagcatga ttattccctc cgtccttctg atgtaaaacg cttacagaac gcagacttag tggtgtggat tggtccggaa atggaagcct ttatggataa gtcgacgcaa agcattgcgg cgaataaaaa ggtgacgatt gccgagctgg atggggtcaa accgctgctc atcaccgggg cggacgacga tgacgatcac catggtcatg gccatggcgc agcagaaaaa ggtgacggcg atcaccatca cggtatttac aatatgcatc tgtggttatc cccagagata gcgcggcttt cggcggttgc aatccacgat aaattattgg aacttatgcc gcagagtcga gccaaactcg acagcaacct gcagcagttc gagaccgcgc tggcggcgac cgacaagcag gtgagcaatg agctggcacc gctgaaaggg aagggctatt tcgtttttca tgacgcctac ggttactttg aaaaacacta cggtttgacc tcgctggggc actttaccgt caaccctgaa atacagcccg gtgcgcagcg tttacacgaa atcagaacac aactggttga gcagaaagcc acttgcgttt ttgctgagcc acagttcagg ccagcggtta tcgaagctgt tgcccggggc acgtctgtgc gcatggggac cctggatcca ctggggacag gaattacgct gggtaaaacg agttacccgc aattcctcac ccaactggcg aatcagtatt cgagctgcct gaaaggagat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat