KlpnC.17353.a

Tryptophan synthase alpha chain

CENTER ID: KlpnC.17353.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.17353.a.B1.GE44315 Full length(KlpnC.17353.a) 1 269
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.2104
UniProt: A0A0H3GRN0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MERYETLFAQ LKNRQEGAFV PFVTLGDPGP EQSLKIIDAL IEGGADALEL GIPFSDPLAD GPTIQGAALR AFAAGVTPAQ CFEMLAAIRQ KHPTIPIGLL MYANLVFSPG IDAFYAQCAR VGVDSVLVAD VPVEESAPFR QAAMRHNIAP IFICPPNADD DLLRQIASYG RGYTYLLSRA GVTGAENRAA LPLHHLVEKL AEYHAAPPLQ GFGISAPEQV SAAIDAGAAG AISGSAIVKI IERHLDEPQT MLDELKAFVQ SLKAATKTA
NT Sequence
ATGGAACGCT ATGAGACGCT GTTTGCCCAA CTGAAAAATC GCCAGGAAGG CGCCTTCGTC CCCTTTGTCA CCCTTGGCGA TCCGGGACCG GAGCAGTCGC TGAAAATTAT CGATGCGCTG ATCGAAGGCG GCGCTGATGC CCTTGAACTG GGGATCCCTT TCTCCGACCC GCTGGCGGAC GGCCCGACCA TCCAGGGCGC GGCCCTGCGC GCCTTCGCCG CGGGAGTGAC CCCGGCGCAG TGCTTTGAGA TGCTGGCGGC GATCCGCCAG AAGCATCCGA CCATCCCAAT CGGCCTGCTG ATGTATGCCA ACCTCGTCTT CAGCCCGGGC ATCGATGCGT TCTATGCCCA GTGCGCCCGC GTCGGCGTCG ACTCGGTGCT GGTGGCCGAC GTGCCGGTGG AAGAGTCCGC CCCGTTCCGC CAGGCGGCGA TGCGCCATAA CATCGCACCG ATTTTCATCT GCCCGCCCAA TGCGGATGAC GATTTACTGC GCCAGATTGC CTCTTATGGC CGCGGCTACA CCTATCTGCT GTCGCGCGCC GGAGTGACGG GTGCGGAAAA TCGCGCCGCG CTGCCGCTGC ACCACCTGGT GGAGAAGCTG GCGGAATATC ACGCCGCGCC GCCGCTGCAG GGCTTTGGTA TCTCCGCGCC GGAGCAGGTG AGCGCCGCCA TTGACGCCGG AGCCGCCGGG GCTATCTCCG GTTCCGCCAT CGTCAAAATC ATCGAACGCC ACCTCGATGA GCCGCAGACC ATGCTCGACG AGCTGAAAGC CTTCGTCCAG AGCCTGAAGG CAGCGACCAA AACCGCC
Details for KlpnC.17353.a.B1.GE44315
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 30kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 88
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHME RYETLFAQLK NRQEGAFVPF VTLGDPGPEQ SLKIIDALIE GGADALELGI PFSDPLADGP TIQGAALRAF AAGVTPAQCF EMLAAIRQKH PTIPIGLLMY ANLVFSPGID AFYAQCARVG VDSVLVADVP VEESAPXXQA AMRHNIAPIF ICPPNADDDL LRQIASYGRG YXYLLSRAGV TGAENRAALP LHHLVEXLAE YHAAPPLXGF GISAPEQVSA AIDAXSRRGL SPVPPSSKSS NATSMXRXPC SXELKAFVRX
Validated NT Sequence
gnnnnnnnnn nnnntatacg actcnctnta gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgg aacgctatga gacgctgttt gcccaactga aaaatcgcca ggaaggcgcc ttcgtcccct ttgtcaccct tggcgatccg ggaccggagc agtcgctgaa aattatcgat gcgctgatcg aaggcggcgc cgatgccctt gaactgggga tccctttctc cgacccgctg gcggacggcc cgaccatcca gggcgcggcc ctgcgcgcct tcgccgcggg agtgaccccg gcgcagtgct ttgagatgct ggcggcgatc cgccagaagc atccgaccat cccaatcggc ctgctgatgt atgccaacct cgtcttcagc ccgggcatcg atgcgttcta tgcccagtgc gcccgcgtcg gcgtcgactc ggtgctggtg gccgacgtgc cggtggaaga gtccgccccn ntncnccagg cggcgatgcg ccataacatc gcgccgattt tcatctgccc gcccaatgcg gatgacgatt tactgcgcca gattgcctct tatggccgcg gctacnccta tctgctgtcg cgcgccggag tgacgggtgc ggaaaatcgc gccgcgctgc cgctgcacca cctggtggag aanctggcgg aatatcacgc cgcgccgccg ctgcanggct ttggtatctc cgcnccngag caagtgagcg ccgccattga cgccngnagc cgccgggggc tatctccggt tccgccntcg tcnaaatcnt cgaacgccac ctcgatganc cgcanaccat gctcnnacga actgaaagcc ttcgtcagan cctgaaaggn agcgaccaaa accgcctaan tgagtaagaa tannntccgg ctgctaacaa agcccgaaag gaagctgant tggctgnctg ccnccgctga gcaataacta gcataacccc ttgggggcnt ctaangggnc ttgnnggggt ttttttgnnt nannnaggaa nnnatattcc nggannnt
Expected Protein Sequence
MAHHHHHHME RYETLFAQLK NRQEGAFVPF VTLGDPGPEQ SLKIIDALIE GGADALELGI PFSDPLADGP TIQGAALRAF AAGVTPAQCF EMLAAIRQKH PTIPIGLLMY ANLVFSPGID AFYAQCARVG VDSVLVADVP VEESAPFRQA AMRHNIAPIF ICPPNADDDL LRQIASYGRG YTYLLSRAGV TGAENRAALP LHHLVEKLAE YHAAPPLQGF GISAPEQVSA AIDAGAAGAI SGSAIVKIIE RHLDEPQTML DELKAFVQSL KAATKTA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac gctatgagac gctgtttgcc caactgaaaa atcgccagga aggcgccttc gtcccctttg tcacccttgg cgatccggga ccggagcagt cgctgaaaat tatcgatgcg ctgatcgaag gcggcgctga tgcccttgaa ctggggatcc ctttctccga cccgctggcg gacggcccga ccatccaggg cgcggccctg cgcgccttcg ccgcgggagt gaccccggcg cagtgctttg agatgctggc ggcgatccgc cagaagcatc cgaccatccc aatcggcctg ctgatgtatg ccaacctcgt cttcagcccg ggcatcgatg cgttctatgc ccagtgcgcc cgcgtcggcg tcgactcggt gctggtggcc gacgtgccgg tggaagagtc cgccccgttc cgccaggcgg cgatgcgcca taacatcgca ccgattttca tctgcccgcc caatgcggat gacgatttac tgcgccagat tgcctcttat ggccgcggct acacctatct gctgtcgcgc gccggagtga cgggtgcgga aaatcgcgcc gcgctgccgc tgcaccacct ggtggagaag ctggcggaat atcacgccgc gccgccgctg cagggctttg gtatctccgc gccggagcag gtgagcgccg ccattgacgc cggagccgcc ggggctatct ccggttccgc catcgtcaaa atcatcgaac gccacctcga tgagccgcag accatgctcg acgagctgaa agccttcgtc cagagcctga aggcagcgac caaaaccgcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat