KlpnC.17337.a
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC
| CENTER ID: | KlpnC.17337.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.17337.a.B1.GE44313 | Full length(KlpnC.17337.a) | 1 | 188 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.17337.a.B1.PW39028 | Full length(KlpnC.17337.a) | 1 | 188 |
Structures
7T35
DEPOSITED: 12/6/2021
DETERMINATION: XRay
CLONE: KlpnC.17337.a.B1.GE44313
PROTEIN: KlpnC.17337.a.B1.PW39028
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.4636 |
| UniProt: | A0A0H3GTS8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNADAQLAT CYGPVSQAFV DRAAKIRLLI LDVDGVLSDG LIYMGNHGEE LKAFNVRDGY GIRCALTSGI EVAIITGRKA KLVEDRCQTL GITHLYQGQS DKLLAFRDLT DKLHVRPEEV AYIGDDLIDW PVMAEVGLSV AVADAHPLLL PRANYVTRIN GGRGAVREVC DLLLLAQGKL DEAKGQSI
NT Sequence
ATGAATAATG CTGATGCTCA GTTAGCGACC TGCTACGGGC CGGTAAGCCA GGCGTTTGTC GATCGCGCGG CGAAGATCCG TCTACTGATC CTTGATGTCG ACGGTGTATT ATCCGACGGG CTTATCTATA TGGGTAATCA CGGCGAGGAG CTGAAAGCGT TTAACGTCCG CGATGGCTAT GGTATCCGCT GCGCGCTGAC CTCGGGTATT GAAGTGGCGA TTATTACCGG GCGAAAAGCT AAACTGGTAG AAGATCGCTG TCAGACGCTG GGCATTACGC ATTTGTACCA GGGGCAGTCG GACAAGCTGC TGGCGTTCCG CGATCTGACG GACAAACTGC ACGTGCGGCC GGAAGAAGTC GCCTACATCG GCGACGATCT TATTGACTGG CCGGTGATGG CCGAAGTGGG CCTTAGCGTC GCCGTCGCCG ATGCGCATCC GCTGCTGCTA CCGCGGGCGA ATTATGTCAC GCGAATTAAC GGCGGACGCG GCGCGGTACG CGAAGTCTGC GATTTGTTAC TATTGGCGCA GGGCAAGCTG GATGAGGCCA AAGGGCAATC AATA
Details for KlpnC.17337.a.B1.GE44313
| HARVESTED ON: | 5/21/2021 |
| SEQUENCED ON: | 6/4/2021 |
| EXPECTED MW: | 21kDa |
| OBSERVED MW: | 21kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHMN NADAQLATCY GPVSQAFVDR AAKIRLLILD VDGVLSDGLI YMGNHGEELK AFNVRDGYGI RCALTSGIEV AIITGRKAKL VEDRCQTLGI THLYQGQSDK LLAFRDLTDK LHVRPEEVAY IGDDLIDWPV MAEVGLSVAV ADAHPLLLPR ANYVTRINGG RGAVRXVCDL LLFXXGQVG
Validated NT Sequence
atcttactca cttatantng atnnccnntn ggcntcatcc aacttgccct gnnncaaata gtaacaaatc gcagacttng cgtaccgcgc cgcgtccgcc gttaattcgc gtgacataat tcgcccgcgg tagcagcagc ggatgcgcat cggcgacggc gacgctaagg cccacttcgg ccatcaccgg ccagtcaatc agatcgtcgc cgatgtaggc gacttcttcc ggccgcacgt gcagtttgtc cgtcagatcg cggaacgcca gcagcttgtc cgactgcccc tggtacaaat gcgtgatgcc cagcgtctga cagcgatctt ctaccagttt agcttttcgc ccggtaataa tcgccacttc aatacccgag gtcagcgcgc agcggatacc atagccatca cggacgttaa acgctttcag ctcctcgccg tgattaccca tatagataag cccgtcggat aatacaccgt cgacatcaag gatcagtaga cggatcttcg ccgcgcgatc gacaaacgcc tggcttaccg gcccgtagca ggtcgctaac tgagcatcag cattattcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMN NADAQLATCY GPVSQAFVDR AAKIRLLILD VDGVLSDGLI YMGNHGEELK AFNVRDGYGI RCALTSGIEV AIITGRKAKL VEDRCQTLGI THLYQGQSDK LLAFRDLTDK LHVRPEEVAY IGDDLIDWPV MAEVGLSVAV ADAHPLLLPR ANYVTRINGG RGAVREVCDL LLLAQGKLDE AKGQSI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata atgctgatgc tcagttagcg acctgctacg ggccggtaag ccaggcgttt gtcgatcgcg cggcgaagat ccgtctactg atccttgatg tcgacggtgt attatccgac gggcttatct atatgggtaa tcacggcgag gagctgaaag cgtttaacgt ccgcgatggc tatggtatcc gctgcgcgct gacctcgggt attgaagtgg cgattattac cgggcgaaaa gctaaactgg tagaagatcg ctgtcagacg ctgggcatta cgcatttgta ccaggggcag tcggacaagc tgctggcgtt ccgcgatctg acggacaaac tgcacgtgcg gccggaagaa gtcgcctaca tcggcgacga tcttattgac tggccggtga tggccgaagt gggccttagc gtcgccgtcg ccgatgcgca tccgctgctg ctaccgcggg cgaattatgt cacgcgaatt aacggcggac gcggcgcggt acgcgaagtc tgcgatttgt tactattggc gcagggcaag ctggatgagg ccaaagggca atcaatatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for KlpnC.17337.a.B1.PW39028
| PURIFICATION DATe: | 6/21/2021 |
| CONCENTRATION: | 29mg/ml |
| OBSERVED MW: | 21kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN NADAQLATCY GPVSQAFVDR AAKIRLLILD VDGVLSDGLI YMGNHGEELK AFNVRDGYGI RCALTSGIEV AIITGRKAKL VEDRCQTLGI THLYQGQSDK LLAFRDLTDK LHVRPEEVAY IGDDLIDWPV MAEVGLSVAV ADAHPLLLPR ANYVTRINGG RGAVRXVCDL LLFXXGQVG
Validated NT Sequence
atcttactca cttatantng atnnccnntn ggcntcatcc aacttgccct gnnncaaata gtaacaaatc gcagacttng cgtaccgcgc cgcgtccgcc gttaattcgc gtgacataat tcgcccgcgg tagcagcagc ggatgcgcat cggcgacggc gacgctaagg cccacttcgg ccatcaccgg ccagtcaatc agatcgtcgc cgatgtaggc gacttcttcc ggccgcacgt gcagtttgtc cgtcagatcg cggaacgcca gcagcttgtc cgactgcccc tggtacaaat gcgtgatgcc cagcgtctga cagcgatctt ctaccagttt agcttttcgc ccggtaataa tcgccacttc aatacccgag gtcagcgcgc agcggatacc atagccatca cggacgttaa acgctttcag ctcctcgccg tgattaccca tatagataag cccgtcggat aatacaccgt cgacatcaag gatcagtaga cggatcttcg ccgcgcgatc gacaaacgcc tggcttaccg gcccgtagca ggtcgctaac tgagcatcag cattattcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMN NADAQLATCY GPVSQAFVDR AAKIRLLILD VDGVLSDGLI YMGNHGEELK AFNVRDGYGI RCALTSGIEV AIITGRKAKL VEDRCQTLGI THLYQGQSDK LLAFRDLTDK LHVRPEEVAY IGDDLIDWPV MAEVGLSVAV ADAHPLLLPR ANYVTRINGG RGAVREVCDL LLLAQGKLDE AKGQSI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA ATGCTGATGC TCAGTTAGCG ACCTGCTACG GGCCGGTAAG CCAGGCGTTT GTCGATCGCG CGGCGAAGAT CCGTCTACTG ATCCTTGATG TCGACGGTGT ATTATCCGAC GGGCTTATCT ATATGGGTAA TCACGGCGAG GAGCTGAAAG CGTTTAACGT CCGCGATGGC TATGGTATCC GCTGCGCGCT GACCTCGGGT ATTGAAGTGG CGATTATTAC CGGGCGAAAA GCTAAACTGG TAGAAGATCG CTGTCAGACG CTGGGCATTA CGCATTTGTA CCAGGGGCAG TCGGACAAGC TGCTGGCGTT CCGCGATCTG ACGGACAAAC TGCACGTGCG GCCGGAAGAA GTCGCCTACA TCGGCGACGA TCTTATTGAC TGGCCGGTGA TGGCCGAAGT GGGCCTTAGC GTCGCCGTCG CCGATGCGCA TCCGCTGCTG CTACCGCGGG CGAATTATGT CACGCGAATT AACGGCGGAC GCGGCGCGGT ACGCGAAGTC TGCGATTTGT TACTATTGGC GCAGGGCAAG CTGGATGAGG CCAAAGGGCA ATCAATATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT