SSGCID: Target Page

KlpnC.17295.a

Glucuronic acid transferase

CENTER ID:	KlpnC.17295.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.17295.a.B1.GE44311	Full length(KlpnC.17295.a)		1	375

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.17295.a.B1.PW39023	Full length(KlpnC.17295.a)		1	375

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.5017
UniProt:	A0A0H3GUS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKFRLALVR QKYRPDGGAE RFVSRALEAL DSSHLQLNVI TREWQGPVKP DWQIHICNPR KWGRISRERG FANAARALWQ RESFDLVQSH ERIPGCDLYR AGDGVHRRWL QQRSRILPAW KSRLLFADRY HRYVMQAERE MYEDSHLRGV ICNAEMIKRE IIEDFGLPAE KIHVIYNAID NQRFLPPDEE TFAALRAKWQ LPLQATCLIY VGSGFERKGL AAAIRAIAPT DRYLLVVGKD KDQPRYQALA KSLNCEARVR FFGMQSETLP FYQMADGLLL PTLYDPFPNV ILEAMACGLP VITTTGCGGA EFIVDGHNGY VCDALDIPAL QQAVMALPAR ALGSAQGGHA RERIMACTSE RLSTQLLSLY QDLVK

NT Sequence

ATGAGTAAAT TCAGGCTGGC TCTGGTGCGG CAGAAGTACC GCCCGGACGG CGGCGCAGAA CGGTTTGTCT CCCGCGCGCT GGAAGCCCTC GACAGCAGTC ATCTGCAACT GAACGTCATC ACCCGCGAAT GGCAGGGGCC GGTGAAACCG GACTGGCAGA TCCATATCTG TAACCCACGT AAATGGGGGC GCATCAGCCG CGAGCGCGGC TTTGCCAACG CCGCGCGCGC GCTCTGGCAG CGCGAGTCCT TCGACCTGGT GCAGAGCCAT GAACGTATTC CCGGCTGCGA TCTCTACCGC GCTGGCGATG GCGTTCATCG CCGCTGGCTG CAGCAGCGCT CGCGCATTTT ACCGGCCTGG AAAAGCCGCC TGCTGTTCGC CGACCGTTAC CACCGCTACG TCATGCAGGC GGAACGCGAG ATGTATGAAG ACTCACACCT GCGCGGGGTG ATCTGCAACG CCGAGATGAT CAAGCGCGAG ATTATCGAAG ACTTTGGCCT GCCGGCGGAG AAGATCCACG TTATTTACAA CGCCATTGAC AACCAGCGCT TCCTGCCGCC AGACGAAGAG ACCTTTGCCG CCTTACGCGC CAAATGGCAG CTGCCGCTGC AGGCGACCTG CCTGATCTAC GTCGGCTCCG GCTTTGAACG TAAAGGGCTG GCGGCGGCGA TCCGCGCCAT CGCTCCCACC GATCGCTACC TGCTGGTGGT CGGCAAAGAT AAGGATCAGC CGCGCTATCA GGCGCTGGCG AAGAGCCTGA ACTGTGAAGC GCGGGTGCGC TTCTTCGGCA TGCAGTCGGA GACATTGCCC TTCTATCAAA TGGCCGATGG TCTGCTGCTG CCCACCCTCT ACGATCCGTT CCCCAACGTC ATCCTCGAGG CAATGGCCTG CGGTCTGCCG GTGATCACCA CTACCGGCTG CGGCGGGGCG GAGTTTATCG TCGACGGCCA CAATGGTTAC GTCTGCGACG CTCTGGATAT CCCGGCGCTA CAGCAGGCGG TAATGGCCCT GCCCGCGCGC GCGCTGGGCT CCGCGCAAGG CGGTCACGCC CGCGAGCGCA TTATGGCCTG CACCAGCGAG CGACTCTCAA CCCAGCTGCT TTCTCTTTAT CAGGATCTGG TGAAA

Details for KlpnC.17295.a.B1.GE44311

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	44kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	90
PERCENT COVERAGE:	56

Validated AA Sequence

MAHHHHHHMS KFRLALVRQK YRPDGGAERF VSRALEALDS SHLQLNVITR EWQGPVKPDW QIHICNPRKW GRISRERGFA NAARALWQRE SFDLVXSHER IPGCDLYRAG DGVHRRWLQQ RSRILPAWKS RLLFADRYHR YVMQAEREMY EDSHLRGVIC NAEMIKREII EDFGLPAEKI HVIYNAIDKP ALPAARXXXL CRLTRXNGSC RCXRPA

Validated NT Sequence

cnnnctcnnn anngggagac cacancggtt tccctctaga aataattttg tttaactttn ncaaggagat ataccatggc tcaccaccac caccaccata tgagtaaatt caggctggct ctggtgcggc agaagtaccg cccggacggc ggcgcagaac ggtttgtctc ccgcgcgctg gaagccctcg acagcagtca tctgcaactg aacgtcatca cccgcgaatg gcaggggccg gtgaaaccgg actggcagat ccatatctgt aacccccgca aatgggggcg catcagccgc gagcgcggct ttgccaacgc cgcgcgcgcg ctctggcagc gcgagtcctt cgacctggtg canagccatg aacgtattcc cggctgcgat ctctaccgcg ctggcgatgg cgttcatcgc cgctggctgc agcagcgctc gcgcatttta ccggcctgga aaagccgcct gctgttcgcc gaccgttacc accgctacgt catgcaggcg gaacgcgaga tgtatgaaga ctcacacctg cgcggggtga tctgcaacgc cgagatgatc aagcgcgaga ttatcgaaga ctttggcctg ccggcggaga agatccacgt tatttacaac gccattgaca aaccagcgct tcctgccgcc agacnaanag anctttgccg ccttacgcgc cnaaatggca gctgccgctg cangcgacct gcctgatcta cgtcggctcc ngctttgaac gtaaagggct ggcggcggcg atccgcgcca tcgctcccac cgatcgctac ctgctggtgg tcggcaaaga taangatcag ccgcgctatc angcgctggc gaanagcctg aactgtgaag cgcgggtgcg cttcttcngc atgcnnncgg annnattgcc cnttctatca anggccgatg gtctgctgct gcccacccct ctacgatccg ttccccaacc ntcntcctcn nngnanngnc ctgcgggncn ngccggnnat ncaccnncnn ccggctggcn gncnggncng nagttantcn ncnnnggccc natgggttac gtctnnnanc ctnctnnnan nttcccngng ccnncancan gnnnggannn nnnnng

Expected Protein Sequence

MAHHHHHHMS KFRLALVRQK YRPDGGAERF VSRALEALDS SHLQLNVITR EWQGPVKPDW QIHICNPRKW GRISRERGFA NAARALWQRE SFDLVQSHER IPGCDLYRAG DGVHRRWLQQ RSRILPAWKS RLLFADRYHR YVMQAEREMY EDSHLRGVIC NAEMIKREII EDFGLPAEKI HVIYNAIDNQ RFLPPDEETF AALRAKWQLP LQATCLIYVG SGFERKGLAA AIRAIAPTDR YLLVVGKDKD QPRYQALAKS LNCEARVRFF GMQSETLPFY QMADGLLLPT LYDPFPNVIL EAMACGLPVI TTTGCGGAEF IVDGHNGYVC DALDIPALQQ AVMALPARAL GSAQGGHARE RIMACTSERL STQLLSLYQD LVK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta aattcaggct ggctctggtg cggcagaagt accgcccgga cggcggcgca gaacggtttg tctcccgcgc gctggaagcc ctcgacagca gtcatctgca actgaacgtc atcacccgcg aatggcaggg gccggtgaaa ccggactggc agatccatat ctgtaaccca cgtaaatggg ggcgcatcag ccgcgagcgc ggctttgcca acgccgcgcg cgcgctctgg cagcgcgagt ccttcgacct ggtgcagagc catgaacgta ttcccggctg cgatctctac cgcgctggcg atggcgttca tcgccgctgg ctgcagcagc gctcgcgcat tttaccggcc tggaaaagcc gcctgctgtt cgccgaccgt taccaccgct acgtcatgca ggcggaacgc gagatgtatg aagactcaca cctgcgcggg gtgatctgca acgccgagat gatcaagcgc gagattatcg aagactttgg cctgccggcg gagaagatcc acgttattta caacgccatt gacaaccagc gcttcctgcc gccagacgaa gagacctttg ccgccttacg cgccaaatgg cagctgccgc tgcaggcgac ctgcctgatc tacgtcggct ccggctttga acgtaaaggg ctggcggcgg cgatccgcgc catcgctccc accgatcgct acctgctggt ggtcggcaaa gataaggatc agccgcgcta tcaggcgctg gcgaagagcc tgaactgtga agcgcgggtg cgcttcttcg gcatgcagtc ggagacattg cccttctatc aaatggccga tggtctgctg ctgcccaccc tctacgatcc gttccccaac gtcatcctcg aggcaatggc ctgcggtctg ccggtgatca ccactaccgg ctgcggcggg gcggagttta tcgtcgacgg ccacaatggt tacgtctgcg acgctctgga tatcccggcg ctacagcagg cggtaatggc cctgcccgcg cgcgcgctgg gctccgcgca aggcggtcac gcccgcgagc gcattatggc ctgcaccagc gagcgactct caacccagct gctttctctt tatcaggatc tggtgaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for KlpnC.17295.a.B1.PW39023

PURIFICATION DATe:	6/14/2021
CONCENTRATION:	15mg/ml
OBSERVED MW:	44kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	90
PERCENT COVERAGE:	56

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA AATTCAGGCT GGCTCTGGTG CGGCAGAAGT ACCGCCCGGA CGGCGGCGCA GAACGGTTTG TCTCCCGCGC GCTGGAAGCC CTCGACAGCA GTCATCTGCA ACTGAACGTC ATCACCCGCG AATGGCAGGG GCCGGTGAAA CCGGACTGGC AGATCCATAT CTGTAACCCA CGTAAATGGG GGCGCATCAG CCGCGAGCGC GGCTTTGCCA ACGCCGCGCG CGCGCTCTGG CAGCGCGAGT CCTTCGACCT GGTGCAGAGC CATGAACGTA TTCCCGGCTG CGATCTCTAC CGCGCTGGCG ATGGCGTTCA TCGCCGCTGG CTGCAGCAGC GCTCGCGCAT TTTACCGGCC TGGAAAAGCC GCCTGCTGTT CGCCGACCGT TACCACCGCT ACGTCATGCA GGCGGAACGC GAGATGTATG AAGACTCACA CCTGCGCGGG GTGATCTGCA ACGCCGAGAT GATCAAGCGC GAGATTATCG AAGACTTTGG CCTGCCGGCG GAGAAGATCC ACGTTATTTA CAACGCCATT GACAACCAGC GCTTCCTGCC GCCAGACGAA GAGACCTTTG CCGCCTTACG CGCCAAATGG CAGCTGCCGC TGCAGGCGAC CTGCCTGATC TACGTCGGCT CCGGCTTTGA ACGTAAAGGG CTGGCGGCGG CGATCCGCGC CATCGCTCCC ACCGATCGCT ACCTGCTGGT GGTCGGCAAA GATAAGGATC AGCCGCGCTA TCAGGCGCTG GCGAAGAGCC TGAACTGTGA AGCGCGGGTG CGCTTCTTCG GCATGCAGTC GGAGACATTG CCCTTCTATC AAATGGCCGA TGGTCTGCTG CTGCCCACCC TCTACGATCC GTTCCCCAAC GTCATCCTCG AGGCAATGGC CTGCGGTCTG CCGGTGATCA CCACTACCGG CTGCGGCGGG GCGGAGTTTA TCGTCGACGG CCACAATGGT TACGTCTGCG ACGCTCTGGA TATCCCGGCG CTACAGCAGG CGGTAATGGC CCTGCCCGCG CGCGCGCTGG GCTCCGCGCA AGGCGGTCAC GCCCGCGAGC GCATTATGGC CTGCACCAGC GAGCGACTCT CAACCCAGCT GCTTTCTCTT TATCAGGATC TGGTGAAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT