SSGCID: Target Page

KlpnC.17267.a

Chaperone SurA

CENTER ID:	KlpnC.17267.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.17267.a.B1.GE44309	Full length(KlpnC.17267.a)		1	428
KlpnC.17267.a.B2.GE44250	Mature protein(KlpnC.17267.a)		20	428

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.17267.a.B2.PW39003	Mature protein(KlpnC.17267.a)		20	428

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.741
UniProt:	A0A0H3GI34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNWKTLLLG IAMIANTSFA APQVVDKVAA VVNNGVVLES DVDGLMQSVK LNAGQAGQQL PDDATLRHQI LERLIMDQIV LQMGQKMGVK ISDDQLDQAI ANIAKQNNMT LDQMRSRLAY EGINYNTYRN QIRKEMLISE VRNNEVRRRI TVLPQEVEAL AKQIGDQNDA STELNLSHIL IPLPENPTSD EVAAAQEQAN SIVEQARNGA NFGKLAITYS ADQQALKGGQ MGWGRIQELP GIFAQALSTA KKGDIVGPIR SGVGFHILKV NDLRGGTQNI SVTEVHARHI LLKPSPIMND AQAQAKLEQI AAEIKSGKIT FAQAAKTYSE DPGSANQGGD LGWATPDIFD PAFRDALMRL NKGQTSGPVH SSFGWHLIEL LDSRQVDRTD AAQKDRAYRM LMNRKFSEEA ATWMQEQRAS AYVKILSN

NT Sequence

ATGAAGAACT GGAAAACGCT GCTTCTCGGT ATCGCCATGA TCGCGAATAC CAGTTTCGCT GCCCCCCAGG TGGTCGATAA AGTAGCGGCC GTCGTCAATA ATGGCGTCGT GCTGGAAAGC GACGTCGATG GTTTGATGCA ATCGGTTAAG CTCAATGCTG GTCAGGCTGG CCAACAGCTG CCGGATGACG CCACCCTGCG CCATCAAATC CTCGAAAGAC TGATCATGGA CCAGATCGTG TTACAGATGG GGCAGAAGAT GGGCGTGAAG ATCTCTGACG ACCAGCTCGA TCAGGCTATC GCCAACATCG CCAAACAAAA TAATATGACC TTGGATCAGA TGCGCAGCCG TCTGGCCTAT GAAGGCATCA ACTACAACAC CTACCGTAAC CAGATCCGTA AAGAGATGCT GATTTCGGAA GTGCGTAACA ATGAAGTGCG TCGTCGCATC ACCGTGCTGC CGCAGGAAGT GGAAGCGCTG GCGAAACAGA TCGGCGACCA GAACGACGCC AGCACCGAGC TCAACCTGAG CCACATCCTG ATCCCGCTGC CGGAGAACCC GACCTCTGAT GAAGTCGCCG CGGCCCAGGA GCAAGCAAAC TCCATCGTTG AGCAGGCGCG CAACGGCGCC AACTTCGGCA AACTGGCGAT CACCTACTCC GCTGACCAAC AGGCGCTGAA GGGTGGCCAG ATGGGCTGGG GCCGTATCCA GGAGCTGCCG GGCATTTTCG CTCAGGCGTT AAGCACGGCG AAGAAAGGGG ATATCGTCGG TCCGATTCGT TCCGGCGTTG GTTTCCACAT TCTGAAAGTC AACGACCTGC GCGGTGGTAC CCAGAATATC TCCGTTACCG AGGTTCACGC CCGTCATATT CTGCTGAAAC CGTCGCCGAT CATGAACGAC GCCCAGGCTC AGGCTAAGCT GGAACAGATC GCAGCCGAGA TTAAGAGCGG GAAAATCACG TTTGCTCAAG CGGCGAAAAC CTATTCGGAA GATCCGGGCT CGGCAAACCA GGGCGGTGAT TTAGGCTGGG CCACGCCGGA CATCTTTGAC CCGGCGTTCC GCGATGCGCT GATGCGTCTG AACAAAGGGC AGACCAGCGG ACCGGTGCAC TCTTCCTTTG GCTGGCATCT GATCGAACTG CTGGATAGCC GTCAGGTCGA CAGAACCGAC GCCGCGCAGA AAGATCGTGC TTACCGCATG CTGATGAACC GCAAATTCTC TGAAGAAGCG GCAACCTGGA TGCAGGAACA GCGCGCCAGT GCGTATGTTA AAATTCTGAG CAAC

Details for KlpnC.17267.a.B1.GE44309

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	53

Validated AA Sequence

Validated NT Sequence

atnnnnncna ctcnntntag ggagaccnnn acggnttccc tctanaaata attttgntna actttnnnnn nggagatata ccatggctca ccaccaccac caccatatga agaactggaa aacgctgctt ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc cccaggtggt cgataaagta gcggccgtcg tcaataatgg cgtcgtgctg gaaagcgacg tcgatggttt gatgcaatcg gttaagctca atgctggtca ggctggccaa cagctgccgg atgacgccac cctgcgccat caaatcctcg aaagactgat catggaccag atcgtgttac agatggggca gaagatgggc gtgaagatct ctgacgacca gctcgatcag gctatcgcca acatcgccaa acaaaataat atgaccttgg atcagatgcg cagccgtctg gcctatgaag gcatcaacta caacacctac cgtaaccaga tccgtaaaga gatgctgatt tcggaagtgc gtaacaatga agtgcgtcgt cgcatcaccg tgctgccgca ggaagtggaa gcgctggcga aacagatcgg cgaccagnac gacgccagca ccgagctcaa cctgagccac atcctgatcc cgctgccgga gaacccgacc tctgatgaag tcgccgcggc ccaggagcaa gcaaactcca tcgttgagca ggcgcgcaac ggcgccaact tcggcaaaac tgggcgatca cctactccgc tgaccaacag gcgctgaagg gtggccagat gggctggggc cgtatccagg agctcccggg cattttcgct cangcgttaa gcncccgcga anaaagggga tatcgtcggt ccgattcgtt cccggcgttg gtttccacat tctgaaagtc aacnacctgc gcgggtggnn acccagaatn nntccgttac cgaggttcnc gcccgtcata atctgctgaa accgtcnccg atctggaacg acgcccagct caggctaagc taacagattn nanncnaann taagancggg aaaatnnngt tttgnntcag cgggnnaaaa cnntatttcn gaannatncg gggttcggcn aannnggggn ggnnant

Expected Protein Sequence

MAHHHHHHMK NWKTLLLGIA MIANTSFAAP QVVDKVAAVV NNGVVLESDV DGLMQSVKLN AGQAGQQLPD DATLRHQILE RLIMDQIVLQ MGQKMGVKIS DDQLDQAIAN IAKQNNMTLD QMRSRLAYEG INYNTYRNQI RKEMLISEVR NNEVRRRITV LPQEVEALAK QIGDQNDAST ELNLSHILIP LPENPTSDEV AAAQEQANSI VEQARNGANF GKLAITYSAD QQALKGGQMG WGRIQELPGI FAQALSTAKK GDIVGPIRSG VGFHILKVND LRGGTQNISV TEVHARHILL KPSPIMNDAQ AQAKLEQIAA EIKSGKITFA QAAKTYSEDP GSANQGGDLG WATPDIFDPA FRDALMRLNK GQTSGPVHSS FGWHLIELLD SRQVDRTDAA QKDRAYRMLM NRKFSEEAAT WMQEQRASAY VKILSN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa taccagtttc gctgcccccc aggtggtcga taaagtagcg gccgtcgtca ataatggcgt cgtgctggaa agcgacgtcg atggtttgat gcaatcggtt aagctcaatg ctggtcaggc tggccaacag ctgccggatg acgccaccct gcgccatcaa atcctcgaaa gactgatcat ggaccagatc gtgttacaga tggggcagaa gatgggcgtg aagatctctg acgaccagct cgatcaggct atcgccaaca tcgccaaaca aaataatatg accttggatc agatgcgcag ccgtctggcc tatgaaggca tcaactacaa cacctaccgt aaccagatcc gtaaagagat gctgatttcg gaagtgcgta acaatgaagt gcgtcgtcgc atcaccgtgc tgccgcagga agtggaagcg ctggcgaaac agatcggcga ccagaacgac gccagcaccg agctcaacct gagccacatc ctgatcccgc tgccggagaa cccgacctct gatgaagtcg ccgcggccca ggagcaagca aactccatcg ttgagcaggc gcgcaacggc gccaacttcg gcaaactggc gatcacctac tccgctgacc aacaggcgct gaagggtggc cagatgggct ggggccgtat ccaggagctg ccgggcattt tcgctcaggc gttaagcacg gcgaagaaag gggatatcgt cggtccgatt cgttccggcg ttggtttcca cattctgaaa gtcaacgacc tgcgcggtgg tacccagaat atctccgtta ccgaggttca cgcccgtcat attctgctga aaccgtcgcc gatcatgaac gacgcccagg ctcaggctaa gctggaacag atcgcagccg agattaagag cgggaaaatc acgtttgctc aagcggcgaa aacctattcg gaagatccgg gctcggcaaa ccagggcggt gatttaggct gggccacgcc ggacatcttt gacccggcgt tccgcgatgc gctgatgcgt ctgaacaaag ggcagaccag cggaccggtg cactcttcct ttggctggca tctgatcgaa ctgctggata gccgtcaggt cgacagaacc gacgccgcgc agaaagatcg tgcttaccgc atgctgatga accgcaaatt ctctgaagaa gcggcaacct ggatgcagga acagcgcgcc agtgcgtatg ttaaaattct gagcaactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for KlpnC.17267.a.B2.GE44250

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	94
PERCENT COVERAGE:	61

Validated AA Sequence

MAHHHHHHMA APQVVDKVAA VVNNGVVLES DVDGLMQSVK LNAGQAGQQL PDDATLRHQI LERLIMDQIV LQMGQKMGVK ISDDQLDQAI ANIAKQNNMT LDQMRSRLAY EGINYNTYRN QIRKEMLISE VRNNEVRRRI TVLPQEVEAL AKQIGDXNDA STELNLSHIL IPLPENPTSD EVAAAQEQAN SIVEQARNGA NFGKLAITYS ADQQALXGGQ MGWGRIQELP GIFAXRXSTA KKGDIVGPDF VPALVSHX

Validated NT Sequence

cactataggg aggaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatggct gccccccagg tggtcgataa agtagcggcc gtcgtcaata atggcgtcgt gctggaaagc gacgtcgatg gtttgatgca atcggttaag ctcaatgctg gtcaggctgg ccaacagctg ccggatgacg ccaccctgcg ccatcaaatc ctcgaaagac tgatcatgga ccagatcgtg ttacagatgg ggcagaagat gggcgtgaag atctctgacg accagctcga tcaggctatc gccaacatcg ccaaacaaaa taatatgacc ttggatcaga tgcgcagccg tctggcctat gaaggcatca actacaacac ctaccgtaac cagatccgta aagagatgct gatttcggaa gtgcgtaaca atgaagtgcg tcgtcgcatc accgtgctgc cgcaggaagt ggaagcgctg gcgaaacaga tcggcgacca naacgacgcc agcaccgagc tcaacctgag ccacatcctg atcccgctgc cggagaaccc gacctctgat gaagtcgccg cggcccagga gcaagcaaac tccatcgttg agcaggcgcg caacggcgcc aacttcggca aactggcgat cacctactcc gctgaccaac aggcgctgaa nggtggncag atgggctggg gccgtatcca ggagctcccg ggcattttcg ctcancgttn aagcaccgcg aagaaagggg atatcgtcgg tcccgatttc gttccggcgt tggtttcaca ttnctgaaag tcaacgacct gcgcggtggt acccagaata tctccgttac cnaggttcac gcccgtcata ttctgctgaa accgtcnncg atcatgaacg anngcccagg ctcangctaa nctaggaaca gatccgcnnn ccganatnag ancgggaaaa tcacngtttg gnncccagcg ggcgaanacc ntattncgga anannccggg ntnnggcaaa ccnngggcgg gngntttaan g

Expected Protein Sequence

MAHHHHHHAA PQVVDKVAAV VNNGVVLESD VDGLMQSVKL NAGQAGQQLP DDATLRHQIL ERLIMDQIVL QMGQKMGVKI SDDQLDQAIA NIAKQNNMTL DQMRSRLAYE GINYNTYRNQ IRKEMLISEV RNNEVRRRIT VLPQEVEALA KQIGDQNDAS TELNLSHILI PLPENPTSDE VAAAQEQANS IVEQARNGAN FGKLAITYSA DQQALKGGQM GWGRIQELPG IFAQALSTAK KGDIVGPIRS GVGFHILKVN DLRGGTQNIS VTEVHARHIL LKPSPIMNDA QAQAKLEQIA AEIKSGKITF AQAAKTYSED PGSANQGGDL GWATPDIFDP AFRDALMRLN KGQTSGPVHS SFGWHLIELL DSRQVDRTDA AQKDRAYRML MNRKFSEEAA TWMQEQRASA YVKILSN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgccc cccaggtggt cgataaagta gcggccgtcg tcaataatgg cgtcgtgctg gaaagcgacg tcgatggttt gatgcaatcg gttaagctca atgctggtca ggctggccaa cagctgccgg atgacgccac cctgcgccat caaatcctcg aaagactgat catggaccag atcgtgttac agatggggca gaagatgggc gtgaagatct ctgacgacca gctcgatcag gctatcgcca acatcgccaa acaaaataat atgaccttgg atcagatgcg cagccgtctg gcctatgaag gcatcaacta caacacctac cgtaaccaga tccgtaaaga gatgctgatt tcggaagtgc gtaacaatga agtgcgtcgt cgcatcaccg tgctgccgca ggaagtggaa gcgctggcga aacagatcgg cgaccagaac gacgccagca ccgagctcaa cctgagccac atcctgatcc cgctgccgga gaacccgacc tctgatgaag tcgccgcggc ccaggagcaa gcaaactcca tcgttgagca ggcgcgcaac ggcgccaact tcggcaaact ggcgatcacc tactccgctg accaacaggc gctgaagggt ggccagatgg gctggggccg tatccaggag ctgccgggca ttttcgctca ggcgttaagc acggcgaaga aaggggatat cgtcggtccg attcgttccg gcgttggttt ccacattctg aaagtcaacg acctgcgcgg tggtacccag aatatctccg ttaccgaggt tcacgcccgt catattctgc tgaaaccgtc gccgatcatg aacgacgccc aggctcaggc taagctggaa cagatcgcag ccgagattaa gagcgggaaa atcacgtttg ctcaagcggc gaaaacctat tcggaagatc cgggctcggc aaaccagggc ggtgatttag gctgggccac gccggacatc tttgacccgg cgttccgcga tgcgctgatg cgtctgaaca aagggcagac cagcggaccg gtgcactctt cctttggctg gcatctgatc gaactgctgg atagccgtca ggtcgacaga accgacgccg cgcagaaaga tcgtgcttac cgcatgctga tgaaccgcaa attctctgaa gaagcggcaa cctggatgca ggaacagcgc gccagtgcgt atgttaaaat tctgagcaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for KlpnC.17267.a.B2.PW39003

PURIFICATION DATe:	5/17/2021
CONCENTRATION:	9mg/ml
OBSERVED MW:	46kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	Chaperone SurA
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	11
VIAL VOLUME:	200µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	61

Protocol Notes

unstable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTGCCC CCCAGGTGGT CGATAAAGTA GCGGCCGTCG TCAATAATGG CGTCGTGCTG GAAAGCGACG TCGATGGTTT GATGCAATCG GTTAAGCTCA ATGCTGGTCA GGCTGGCCAA CAGCTGCCGG ATGACGCCAC CCTGCGCCAT CAAATCCTCG AAAGACTGAT CATGGACCAG ATCGTGTTAC AGATGGGGCA GAAGATGGGC GTGAAGATCT CTGACGACCA GCTCGATCAG GCTATCGCCA ACATCGCCAA ACAAAATAAT ATGACCTTGG ATCAGATGCG CAGCCGTCTG GCCTATGAAG GCATCAACTA CAACACCTAC CGTAACCAGA TCCGTAAAGA GATGCTGATT TCGGAAGTGC GTAACAATGA AGTGCGTCGT CGCATCACCG TGCTGCCGCA GGAAGTGGAA GCGCTGGCGA AACAGATCGG CGACCAGAAC GACGCCAGCA CCGAGCTCAA CCTGAGCCAC ATCCTGATCC CGCTGCCGGA GAACCCGACC TCTGATGAAG TCGCCGCGGC CCAGGAGCAA GCAAACTCCA TCGTTGAGCA GGCGCGCAAC GGCGCCAACT TCGGCAAACT GGCGATCACC TACTCCGCTG ACCAACAGGC GCTGAAGGGT GGCCAGATGG GCTGGGGCCG TATCCAGGAG CTGCCGGGCA TTTTCGCTCA GGCGTTAAGC ACGGCGAAGA AAGGGGATAT CGTCGGTCCG ATTCGTTCCG GCGTTGGTTT CCACATTCTG AAAGTCAACG ACCTGCGCGG TGGTACCCAG AATATCTCCG TTACCGAGGT TCACGCCCGT CATATTCTGC TGAAACCGTC GCCGATCATG AACGACGCCC AGGCTCAGGC TAAGCTGGAA CAGATCGCAG CCGAGATTAA GAGCGGGAAA ATCACGTTTG CTCAAGCGGC GAAAACCTAT TCGGAAGATC CGGGCTCGGC AAACCAGGGC GGTGATTTAG GCTGGGCCAC GCCGGACATC TTTGACCCGG CGTTCCGCGA TGCGCTGATG CGTCTGAACA AAGGGCAGAC CAGCGGACCG GTGCACTCTT CCTTTGGCTG GCATCTGATC GAACTGCTGG ATAGCCGTCA GGTCGACAGA ACCGACGCCG CGCAGAAAGA TCGTGCTTAC CGCATGCTGA TGAACCGCAA ATTCTCTGAA GAAGCGGCAA CCTGGATGCA GGAACAGCGC GCCAGTGCGT ATGTTAAAAT TCTGAGCAAC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT