SSGCID: Target Page

KlpnC.10478.a

4-amino-4-deoxychorismate lyase

CENTER ID:	KlpnC.10478.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.10478.a.B1.GE44306	Full length(KlpnC.10478.a)		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.10478.a.B1.PW39029	Full length(KlpnC.10478.a)		1	269

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.1923
UniProt:	A0A0H3GRH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFLINGVVQD TLAANDRATQ FGDGCFTTAR IQQGQVALLD AHLQRLQTTC EKLHIPFNDW LTLSEEMQRL ARPHAQGVLK VTLTRGVGGR GYSTAGCVSP TRILSFSPFP AHYARWREEG ITLTQSPVPL GRNSWLAGLK HLNRLEQVLI RSHLEQTDAD EALVLDSDGW LTECCAANLF WRQGRDVFTP RLDYAGVNGI MRQRCIAQLA PSTFRVVEVT ARPETLREAD EVLICNALMP LVPVRRWEET TWSSRELYHF LAPLCELSG

NT Sequence

ATGTTCTTGA TAAACGGCGT TGTACAGGAC ACGTTGGCAG CGAACGATCG CGCCACCCAG TTTGGCGACG GCTGTTTTAC CACCGCCCGC ATCCAGCAAG GGCAGGTTGC GCTACTGGAC GCCCATCTGC AGCGTTTACA GACAACCTGT GAAAAATTAC ATATCCCATT TAACGACTGG CTGACGCTGA GCGAAGAGAT GCAGCGTCTG GCTCGGCCGC ACGCGCAGGG CGTGCTCAAG GTGACTCTCA CCCGCGGCGT TGGGGGAAGG GGATACAGTA CGGCAGGCTG CGTGTCGCCC ACTCGCATTC TCAGCTTCTC TCCATTCCCG GCGCATTATG CGCGCTGGCG GGAGGAGGGC ATTACTCTGA CCCAGAGTCC GGTGCCCCTG GGGCGCAACT CCTGGCTTGC CGGCCTGAAG CACCTTAATC GCCTTGAGCA GGTGCTGATC CGCTCTCATC TTGAGCAGAC GGACGCCGAT GAGGCGCTGG TCCTTGACAG TGACGGGTGG CTTACGGAAT GCTGTGCCGC GAATTTATTC TGGCGTCAGG GACGTGATGT ATTTACTCCG CGACTCGATT ATGCCGGGGT TAACGGCATT ATGCGGCAGC GCTGTATCGC GCAGCTGGCA CCATCGACGT TTCGCGTTGT CGAAGTGACA GCGCGTCCCG AGACGTTGCG GGAGGCCGAT GAAGTGCTGA TCTGCAATGC GCTGATGCCG CTGGTGCCGG TTCGCCGCTG GGAGGAGACC ACCTGGTCCT CGCGTGAGCT TTACCATTTT TTAGCCCCTT TGTGTGAGCT GTCTGGT

Details for KlpnC.10478.a.B1.GE44306

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	31kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	92
PERCENT COVERAGE:	81

Validated AA Sequence

MAHHHHHHMF LINGVVQDTL AANDRATQFG DGCFTTARIQ QGQVALLDAH LQRLQTTCEK LHIPFNDWLT LSEEMQRLAR PHAQGVLKVT LTRGVGGRGY STAGCVSPTR ILSFSPFPAH YARWREEGIT LTQSPVPLGR NSWLXGLKHL NRLEQVLIRS HLEQTDADXA LVLDSDGWLT ECCAANLFWR QGRDVFTPRL DYAGVNGIYA XALYRAAGTI DVFALSK

Validated NT Sequence

antcnctata gggagaccac aaccggtttc cctctagaaa taattttgtt taactttann aaggagatat accatggctc accaccacca ccaccatatg ttcttgataa acggcgttgt acaggacacg ttggcagcga acgatcgcgc cacccagttt ggcgacggct gttttaccac cgcccgcatc cagcaagggc aggttgcgct actggacgcc catctgcagc gtttgcagac aacctgtgaa aaattacata tcccatttaa cgactggctg acgctgagcg aagagatgca gcgtctggct cggccgcacg cgcagggcgt gctcaaggtg actctcaccc gcggcgttgg gggaagggga tacagtacgg caggctgcgt gtcgcccact cgcattctca gcttctctcc attcccggcg cattatgcgc gctggcggga ggagggcatt actctgaccc agagtccggt gcccctgggg cgcaactcct ggcttgncgg cctgaagcac cttaatcgcc ttgagcaggt gctgatccgc tctcatcttg agcagacgga cgccgatgan gcgctggtcc ttgacagtga cgggtggctt acggaatgct gtgccgcgaa tttattctgg cgtcagggac gtgatgtatt tactccgcga ctcgattatg ccggggtcaa cggcatttat gcggnagcgc tgtatcgcgc agctggcacc atcgacgttt tcgcgttgtc gaagtgacag cgcgtcccga nacantgcgg gaggccgatg aagtgctgat ctgcaatgcg ctgatgccgc tggtgccgga tcgccgctgg gagnagacca cntggttctc gcgtgagctt taccnttttt ancccctttg tgtgagctgn ctggtttant gagtagatag gatccggctg ctnacaaagc ccgaaagaan ctgagttggc tgctgcanng ctgancanaa ctancattaa ccccttgggg ccnctnaacg gtnttga

Expected Protein Sequence

MAHHHHHHMF LINGVVQDTL AANDRATQFG DGCFTTARIQ QGQVALLDAH LQRLQTTCEK LHIPFNDWLT LSEEMQRLAR PHAQGVLKVT LTRGVGGRGY STAGCVSPTR ILSFSPFPAH YARWREEGIT LTQSPVPLGR NSWLAGLKHL NRLEQVLIRS HLEQTDADEA LVLDSDGWLT ECCAANLFWR QGRDVFTPRL DYAGVNGIMR QRCIAQLAPS TFRVVEVTAR PETLREADEV LICNALMPLV PVRRWEETTW SSRELYHFLA PLCELSG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttct tgataaacgg cgttgtacag gacacgttgg cagcgaacga tcgcgccacc cagtttggcg acggctgttt taccaccgcc cgcatccagc aagggcaggt tgcgctactg gacgcccatc tgcagcgttt acagacaacc tgtgaaaaat tacatatccc atttaacgac tggctgacgc tgagcgaaga gatgcagcgt ctggctcggc cgcacgcgca gggcgtgctc aaggtgactc tcacccgcgg cgttggggga aggggataca gtacggcagg ctgcgtgtcg cccactcgca ttctcagctt ctctccattc ccggcgcatt atgcgcgctg gcgggaggag ggcattactc tgacccagag tccggtgccc ctggggcgca actcctggct tgccggcctg aagcacctta atcgccttga gcaggtgctg atccgctctc atcttgagca gacggacgcc gatgaggcgc tggtccttga cagtgacggg tggcttacgg aatgctgtgc cgcgaattta ttctggcgtc agggacgtga tgtatttact ccgcgactcg attatgccgg ggttaacggc attatgcggc agcgctgtat cgcgcagctg gcaccatcga cgtttcgcgt tgtcgaagtg acagcgcgtc ccgagacgtt gcgggaggcc gatgaagtgc tgatctgcaa tgcgctgatg ccgctggtgc cggttcgccg ctgggaggag accacctggt cctcgcgtga gctttaccat tttttagccc ctttgtgtga gctgtctggt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for KlpnC.10478.a.B1.PW39029

PURIFICATION DATe:	6/22/2021
CONCENTRATION:	63mg/ml
OBSERVED MW:	28kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	2µl
PERCENT IDENTITY:	92
PERCENT COVERAGE:	81

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCT TGATAAACGG CGTTGTACAG GACACGTTGG CAGCGAACGA TCGCGCCACC CAGTTTGGCG ACGGCTGTTT TACCACCGCC CGCATCCAGC AAGGGCAGGT TGCGCTACTG GACGCCCATC TGCAGCGTTT ACAGACAACC TGTGAAAAAT TACATATCCC ATTTAACGAC TGGCTGACGC TGAGCGAAGA GATGCAGCGT CTGGCTCGGC CGCACGCGCA GGGCGTGCTC AAGGTGACTC TCACCCGCGG CGTTGGGGGA AGGGGATACA GTACGGCAGG CTGCGTGTCG CCCACTCGCA TTCTCAGCTT CTCTCCATTC CCGGCGCATT ATGCGCGCTG GCGGGAGGAG GGCATTACTC TGACCCAGAG TCCGGTGCCC CTGGGGCGCA ACTCCTGGCT TGCCGGCCTG AAGCACCTTA ATCGCCTTGA GCAGGTGCTG ATCCGCTCTC ATCTTGAGCA GACGGACGCC GATGAGGCGC TGGTCCTTGA CAGTGACGGG TGGCTTACGG AATGCTGTGC CGCGAATTTA TTCTGGCGTC AGGGACGTGA TGTATTTACT CCGCGACTCG ATTATGCCGG GGTTAACGGC ATTATGCGGC AGCGCTGTAT CGCGCAGCTG GCACCATCGA CGTTTCGCGT TGTCGAAGTG ACAGCGCGTC CCGAGACGTT GCGGGAGGCC GATGAAGTGC TGATCTGCAA TGCGCTGATG CCGCTGGTGC CGGTTCGCCG CTGGGAGGAG ACCACCTGGT CCTCGCGTGA GCTTTACCAT TTTTTAGCCC CTTTGTGTGA GCTGTCTGGT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT