KlpnC.01328.a
3-deoxy-manno-octulosonate cytidylyltransferase
| CENTER ID: | KlpnC.01328.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.01328.a.B1.GE44299 | Full length(KlpnC.01328.a) | 1 | 245 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.01328.a.B1.PW39074 | Full length(KlpnC.01328.a) | 1 | 245 |
Structures
8EES
DEPOSITED: 9/7/2022
DETERMINATION: XRay
CLONE: KlpnC.01328.a.B1.GE44299
PROTEIN: KlpnC.01328.a.B1.PW39074
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.1777 |
| UniProt: | A0A0H3GMB7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVIIPARFAS TRLPGKPLQD INGKPMIVHV LERARESGAD RIIVATDHED VARAVEAAGG EVCMTRADHQ SGTERLAEVV EKCAFSDDTI IVNIQGDEPM IPPAIVRQVA ENLAASSSGM ATLAVPIHDA EEAFNPNAVK VVMDAKGYAL YFSRATIPWD RDRFAQSRET IGDSLLRHIG IYGYRAGFIR RYVSWAPSPL EQIEMLEQLR VLWYGEKIHV AVAAEVPGTG VDTPEDLERV RAELR
NT Sequence
GTGGTCATTA TTCCTGCGCG TTTCGCCTCC ACGCGCCTGC CGGGAAAACC ACTGCAGGAC ATTAATGGTA AGCCGATGAT TGTCCATGTG CTGGAGCGCG CTCGCGAATC CGGCGCGGAT CGCATTATTG TCGCCACCGA TCACGAAGAT GTCGCCCGGG CGGTTGAAGC CGCCGGCGGC GAGGTCTGTA TGACCCGCGC CGACCATCAG TCCGGCACCG AACGGCTGGC GGAAGTGGTG GAAAAGTGCG CGTTCAGCGA TGACACCATT ATCGTCAATA TTCAGGGCGA TGAGCCGATG ATCCCCCCGG CGATTGTCCG CCAGGTGGCG GAGAATCTGG CTGCCAGCAG CAGCGGAATG GCGACCCTGG CGGTACCGAT CCATGATGCG GAAGAGGCGT TTAACCCGAA TGCGGTCAAA GTGGTGATGG ATGCCAAGGG TTACGCGCTC TATTTCTCCC GCGCGACTAT TCCCTGGGAT CGCGATCGCT TTGCCCAATC CCGCGAGACC ATCGGTGACT CCCTGCTGCG TCACATCGGC ATCTATGGCT ATCGCGCCGG CTTTATCCGC CGCTATGTCA GCTGGGCGCC GAGCCCGCTG GAGCAGATTG AAATGCTCGA GCAGCTGCGA GTGCTGTGGT ATGGCGAGAA AATCCATGTC GCCGTGGCGG CCGAAGTGCC CGGCACCGGC GTGGATACCC CGGAAGATCT GGAGCGCGTC CGCGCTGAGC TGCGT
Details for KlpnC.01328.a.B1.GE44299
| HARVESTED ON: | 5/21/2021 |
| SEQUENCED ON: | 6/4/2021 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMV VIIPARFAST RLPGKPLQDI NGKPMIVHVL ERARESGADR IIVATDHEDV ARAVEAAGGE VCMTRADHQS GTERLAEVVE KCAFSDDTII VNIQGDEPMI PPAIVRQVAE NLAASSSGMA TLAVPIHDAE EAFNPNAVKV VMDAKGYALY FSRATIPWDR DRFAQSRETI GDSLLRHIGI YGYRAGFIRR YVSWAPSPLE XIEMLEQLRX LWYGEKIHVA VAAEVARHRR GYPGRXGXRP R
Validated NT Sequence
actcnctata gggagaccan caacggtttc cctctagaaa taattttgtt taactttann naaggagata taccatggct caccaccacc accaccatat ggtggtcatt attcctgcgc gtttcgcctc cacgcgcctg ccgggaaaac cactgcagga cattaatggt aagccgatga ttgtccatgt gctggagcgc gctcgcgaat ccggcgcgga tcgcattatt gtcgccaccg atcacgaaga tgtcgcccgg gcggttgaag ccgccggcgg cgaggtctgt atgacccgcg ccgaccatca gtccggcacc gaacggctgg cggaagtggt ggaaaagtgc gcgttcagcg atgacaccat tatcgtcaat attcagggcg atgagccgat gatccccccg gcgattgtcc gccaggtggc ggagaatctg gctgccagca gcagcggaat ggcgacgctg gcggtaccga tccatgatgc ggaagaggcg tttaacccga atgcggtcaa agtggtgatg gatgccaagg gttacgcgct ctatttctcc cgcgcgacta ttccctggga tcgcgatcgc tttgcccaat cccgcgagac catcggngac tccctgctgc gtcacatcgg catctatggc tatcgcgccg gctttatccg ccgctatgtc agctgggcgc cnagcccgct ggagcanatt gaaatgctcg agcagctgcg antgctgtgg tatggcgaga aaatccatgt cgccgtggcg gcggaagttg cccggcaccg gcgtggatac cccggaagan ctggancgcg tccncgctga actgcntnan tgagttanan agntccgnct gctaacaagc cgaagtnant tgntgctgcc ncgntganat actannnacc ccntggggcn tcnaacgggt ctgaggtttt tgctgaaann naactnannt cnnannncnn nnnggnnngg ncncnngatc nnnnnnnann nnnnnnnann nnnnnnnnan tggnggggcn anggtngann gnncnnaang ggngncnnna ant
Expected Protein Sequence
MAHHHHHHMV IIPARFASTR LPGKPLQDIN GKPMIVHVLE RARESGADRI IVATDHEDVA RAVEAAGGEV CMTRADHQSG TERLAEVVEK CAFSDDTIIV NIQGDEPMIP PAIVRQVAEN LAASSSGMAT LAVPIHDAEE AFNPNAVKVV MDAKGYALYF SRATIPWDRD RFAQSRETIG DSLLRHIGIY GYRAGFIRRY VSWAPSPLEQ IEMLEQLRVL WYGEKIHVAV AAEVPGTGVD TPEDLERVRA ELR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggtca ttattcctgc gcgtttcgcc tccacgcgcc tgccgggaaa accactgcag gacattaatg gtaagccgat gattgtccat gtgctggagc gcgctcgcga atccggcgcg gatcgcatta ttgtcgccac cgatcacgaa gatgtcgccc gggcggttga agccgccggc ggcgaggtct gtatgacccg cgccgaccat cagtccggca ccgaacggct ggcggaagtg gtggaaaagt gcgcgttcag cgatgacacc attatcgtca atattcaggg cgatgagccg atgatccccc cggcgattgt ccgccaggtg gcggagaatc tggctgccag cagcagcgga atggcgaccc tggcggtacc gatccatgat gcggaagagg cgtttaaccc gaatgcggtc aaagtggtga tggatgccaa gggttacgcg ctctatttct cccgcgcgac tattccctgg gatcgcgatc gctttgccca atcccgcgag accatcggtg actccctgct gcgtcacatc ggcatctatg gctatcgcgc cggctttatc cgccgctatg tcagctgggc gccgagcccg ctggagcaga ttgaaatgct cgagcagctg cgagtgctgt ggtatggcga gaaaatccat gtcgccgtgg cggccgaagt gcccggcacc ggcgtggata ccccggaaga tctggagcgc gtccgcgctg agctgcgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for KlpnC.01328.a.B1.PW39074
| PURIFICATION DATe: | 8/2/2021 |
| CONCENTRATION: | 14mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV VIIPARFAST RLPGKPLQDI NGKPMIVHVL ERARESGADR IIVATDHEDV ARAVEAAGGE VCMTRADHQS GTERLAEVVE KCAFSDDTII VNIQGDEPMI PPAIVRQVAE NLAASSSGMA TLAVPIHDAE EAFNPNAVKV VMDAKGYALY FSRATIPWDR DRFAQSRETI GDSLLRHIGI YGYRAGFIRR YVSWAPSPLE XIEMLEQLRX LWYGEKIHVA VAAEVARHRR GYPGRXGXRP R
Validated NT Sequence
actcnctata gggagaccan caacggtttc cctctagaaa taattttgtt taactttann naaggagata taccatggct caccaccacc accaccatat ggtggtcatt attcctgcgc gtttcgcctc cacgcgcctg ccgggaaaac cactgcagga cattaatggt aagccgatga ttgtccatgt gctggagcgc gctcgcgaat ccggcgcgga tcgcattatt gtcgccaccg atcacgaaga tgtcgcccgg gcggttgaag ccgccggcgg cgaggtctgt atgacccgcg ccgaccatca gtccggcacc gaacggctgg cggaagtggt ggaaaagtgc gcgttcagcg atgacaccat tatcgtcaat attcagggcg atgagccgat gatccccccg gcgattgtcc gccaggtggc ggagaatctg gctgccagca gcagcggaat ggcgacgctg gcggtaccga tccatgatgc ggaagaggcg tttaacccga atgcggtcaa agtggtgatg gatgccaagg gttacgcgct ctatttctcc cgcgcgacta ttccctggga tcgcgatcgc tttgcccaat cccgcgagac catcggngac tccctgctgc gtcacatcgg catctatggc tatcgcgccg gctttatccg ccgctatgtc agctgggcgc cnagcccgct ggagcanatt gaaatgctcg agcagctgcg antgctgtgg tatggcgaga aaatccatgt cgccgtggcg gcggaagttg cccggcaccg gcgtggatac cccggaagan ctggancgcg tccncgctga actgcntnan tgagttanan agntccgnct gctaacaagc cgaagtnant tgntgctgcc ncgntganat actannnacc ccntggggcn tcnaacgggt ctgaggtttt tgctgaaann naactnannt cnnannncnn nnnggnnngg ncncnngatc nnnnnnnann nnnnnnnann nnnnnnnnan tggnggggcn anggtngann gnncnnaang ggngncnnna ant
Expressed Protein Sequence
MAHHHHHHMV IIPARFASTR LPGKPLQDIN GKPMIVHVLE RARESGADRI IVATDHEDVA RAVEAAGGEV CMTRADHQSG TERLAEVVEK CAFSDDTIIV NIQGDEPMIP PAIVRQVAEN LAASSSGMAT LAVPIHDAEE AFNPNAVKVV MDAKGYALYF SRATIPWDRD RFAQSRETIG DSLLRHIGIY GYRAGFIRRY VSWAPSPLEQ IEMLEQLRVL WYGEKIHVAV AAEVPGTGVD TPEDLERVRA ELR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGTCA TTATTCCTGC GCGTTTCGCC TCCACGCGCC TGCCGGGAAA ACCACTGCAG GACATTAATG GTAAGCCGAT GATTGTCCAT GTGCTGGAGC GCGCTCGCGA ATCCGGCGCG GATCGCATTA TTGTCGCCAC CGATCACGAA GATGTCGCCC GGGCGGTTGA AGCCGCCGGC GGCGAGGTCT GTATGACCCG CGCCGACCAT CAGTCCGGCA CCGAACGGCT GGCGGAAGTG GTGGAAAAGT GCGCGTTCAG CGATGACACC ATTATCGTCA ATATTCAGGG CGATGAGCCG ATGATCCCCC CGGCGATTGT CCGCCAGGTG GCGGAGAATC TGGCTGCCAG CAGCAGCGGA ATGGCGACCC TGGCGGTACC GATCCATGAT GCGGAAGAGG CGTTTAACCC GAATGCGGTC AAAGTGGTGA TGGATGCCAA GGGTTACGCG CTCTATTTCT CCCGCGCGAC TATTCCCTGG GATCGCGATC GCTTTGCCCA ATCCCGCGAG ACCATCGGTG ACTCCCTGCT GCGTCACATC GGCATCTATG GCTATCGCGC CGGCTTTATC CGCCGCTATG TCAGCTGGGC GCCGAGCCCG CTGGAGCAGA TTGAAATGCT CGAGCAGCTG CGAGTGCTGT GGTATGGCGA GAAAATCCAT GTCGCCGTGG CGGCCGAAGT GCCCGGCACC GGCGTGGATA CCCCGGAAGA TCTGGAGCGC GTCCGCGCTG AGCTGCGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT