KlpnC.01254.a

Coenzyme A biosynthesis bifunctional protein CoaBC

CENTER ID: KlpnC.01254.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.01254.a.B1.GE44297 Full length(KlpnC.01254.a) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.5027
UniProt: A0A0H3GUS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMTLAGKKIV LGVSGGIAAY KTPELVRRLR ERGAEVRVAM TEAAKAFITP LSLQAVSGYP VSDSLLDPAA EAAMGHIELG KWADLVILAP ATADLIARVA AGMANDLVST ICLATPSPVA VVPAMNQQMY RAQATQHNLQ TLATRGLLLW GPDSGSQACG DVGPGRMLDP LTIVDMAAQH FASPVKDLQH LNLMITAGPT REPLDPVRYI TNHSSGKMGF AIAAAAAQRG ANVTLISGPV SLPTPPFVQR IDVTTALEME AAVQAGAQQQ HIFIGCAAVA DYRAAVIAED KIKKQGDELT IKMVKNPDIV AGVAALKSHR PYVVGFAAET NYVEEYARQK RARKNLDLIC ANDVSQPNQG FNSDSNALHL FWQDGEKRLP LERKELLGQL LLDEIVTRYD EKNRR
NT Sequence
ATGATGACTC TGGCGGGTAA AAAAATCGTC CTGGGCGTCA GCGGCGGCAT CGCGGCGTAT AAAACCCCGG AACTGGTGCG CCGGCTGCGT GAACGCGGCG CGGAAGTGCG TGTCGCGATG ACGGAGGCGG CAAAAGCGTT CATCACCCCA CTCAGCCTGC AGGCGGTATC CGGCTATCCG GTTTCCGACA GCCTGCTCGA TCCGGCGGCG GAAGCGGCAA TGGGCCATAT TGAGCTCGGT AAATGGGCCG ACCTGGTGAT CCTCGCCCCA GCGACCGCCG ATTTAATCGC TCGCGTGGCG GCCGGCATGG CCAACGATCT GGTGTCGACG ATCTGTCTTG CCACTCCATC CCCGGTTGCC GTGGTGCCCG CCATGAACCA GCAGATGTAT CGCGCGCAGG CCACCCAGCA TAATCTGCAG ACGCTCGCTA CTCGCGGGCT GCTGCTATGG GGACCAGACA GCGGTAGCCA GGCCTGCGGC GACGTCGGCC CGGGGAGAAT GCTCGATCCG CTAACTATCG TAGACATGGC CGCGCAGCAT TTCGCCTCCC CTGTCAAAGA TTTGCAACAT CTCAACCTCA TGATTACCGC GGGCCCGACC CGTGAGCCAT TGGATCCGGT GCGTTATATC ACCAACCACA GCTCTGGCAA GATGGGCTTC GCGATTGCCG CCGCCGCAGC GCAGCGGGGC GCGAACGTGA CCTTGATCAG CGGGCCGGTA TCGCTACCGA CCCCGCCGTT TGTGCAGCGC ATTGATGTCA CTACCGCGCT GGAGATGGAA GCGGCCGTCC AGGCTGGCGC CCAGCAGCAG CACATTTTTA TTGGCTGCGC CGCAGTAGCG GATTACCGCG CCGCCGTCAT CGCCGAAGAT AAAATAAAAA AACAAGGTGA TGAATTAACA ATAAAAATGG TTAAAAACCC GGATATAGTG GCCGGGGTCG CGGCGCTAAA ATCACATCGC CCCTACGTCG TTGGGTTTGC CGCCGAAACG AATTATGTGG AAGAATATGC GCGGCAAAAA CGTGCCCGCA AGAACCTCGA CCTGATTTGC GCCAACGATG TTTCCCAGCC TAATCAGGGC TTTAACAGCG ACAGCAACGC ACTACACCTT TTCTGGCAGG ATGGGGAAAA GCGCTTACCA CTTGAGCGCA AGGAACTCCT GGGCCAATTA TTACTCGACG AGATCGTGAC CCGTTATGAT GAAAAAAATC GACGT
Details for KlpnC.01254.a.B1.GE44297
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 45kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 71
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHMM TLAGKKIVLG VSGGIAAYKT PELVRRLRER GAEVRVAMTE AAKAFITPLS LQAVSGYPVS DSLLDPAAEA AMGHIELGKW ADLVILAPAT ADLIARVAAG MANDLVSTIC LATPSPVAVV PAMNQQMYRA QATQHNLQTL ATRGLLLWGP DSGSQACGDV GPGRMLDPLT IVDMAAQHFA SPVXDLQHLN LMITAGPTRE PLDPVRYITX HSSGXMGFXI AAXRSAAGRE CDLDQRAGIA TDPAVCAAHL MSLPRWXWKR XPGWXPXXAH FYWLRPVAXY XAXSSPRXXX KNXXWXXIKX XKPGXSXXGR XXKXHRPXXX GXPPKXXXXX XX
Validated NT Sequence
gactcnctat agggagacca caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg atgactctgg cgggtaaaaa aatcgtcctg ggcgtcagcg gcggcatcgc ggcgtataaa accccggaac tggtgcgccg gctgcgtgaa cgcggcgcgg aagtgcgtgt cgcgatgacg gaggcggcaa aagcgttcat caccccactc agcctgcagg cggtatccgg ctatccggtt tccgacagcc tgctcgatcc ggcggcggaa gcggcaatgg gccatattga gctcggtaaa tgggccgacc tggtgatcct cgccccagcg accgccgatt taatcgctcg cgtggcggcc ggcatggcca acgatctggt gtcgacgatc tgtcttgcca ctccatcccc ggttgccgtg gtgcccgcca tgaaccagca gatgtatcgc gcgcaggcca cccagcataa tctgcagacg ctcgctactc gcgggctgct gctatgggga ccagacagcg gtagccaggc ctgcggcgac gtcggcccgg ggagaatgct cgatccgcta actatcgtag acatggccgc gcagcatttc gcctcccctg tcnaagattt gcaacatctc aacctcatga ttaccgcggg cccgacccgt gagccattgg atccggtgcg ttatatcacc naccacagct ctggcaanat gggcttcncg attgccgccg nccgcagcgc agcggggcgc gaatgtgacc ttgatcagcg ggccggtatc gctaccgacc ccgccgtttg tgcagcgcat ttgatgtcac taccgcgctg ganatggaag cgnngtccag gctggcnccc ancancagca catttttatt ggctgcgccc ggtagcgnat tacngcgccn cgtcatcncc gaganaaaan naaaaaaacn nnngatggan tanaataaaa nggnnaaaac ccggannatc gtnnnngggt cgcngnncta aancncatcg ncccnacnnc ntgggnntnc cnccgaaacn atnangnnnn naaanannnc
Expected Protein Sequence
MAHHHHHHMM TLAGKKIVLG VSGGIAAYKT PELVRRLRER GAEVRVAMTE AAKAFITPLS LQAVSGYPVS DSLLDPAAEA AMGHIELGKW ADLVILAPAT ADLIARVAAG MANDLVSTIC LATPSPVAVV PAMNQQMYRA QATQHNLQTL ATRGLLLWGP DSGSQACGDV GPGRMLDPLT IVDMAAQHFA SPVKDLQHLN LMITAGPTRE PLDPVRYITN HSSGKMGFAI AAAAAQRGAN VTLISGPVSL PTPPFVQRID VTTALEMEAA VQAGAQQQHI FIGCAAVADY RAAVIAEDKI KKQGDELTIK MVKNPDIVAG VAALKSHRPY VVGFAAETNY VEEYARQKRA RKNLDLICAN DVSQPNQGFN SDSNALHLFW QDGEKRLPLE RKELLGQLLL DEIVTRYDEK NRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatga ctctggcggg taaaaaaatc gtcctgggcg tcagcggcgg catcgcggcg tataaaaccc cggaactggt gcgccggctg cgtgaacgcg gcgcggaagt gcgtgtcgcg atgacggagg cggcaaaagc gttcatcacc ccactcagcc tgcaggcggt atccggctat ccggtttccg acagcctgct cgatccggcg gcggaagcgg caatgggcca tattgagctc ggtaaatggg ccgacctggt gatcctcgcc ccagcgaccg ccgatttaat cgctcgcgtg gcggccggca tggccaacga tctggtgtcg acgatctgtc ttgccactcc atccccggtt gccgtggtgc ccgccatgaa ccagcagatg tatcgcgcgc aggccaccca gcataatctg cagacgctcg ctactcgcgg gctgctgcta tggggaccag acagcggtag ccaggcctgc ggcgacgtcg gcccggggag aatgctcgat ccgctaacta tcgtagacat ggccgcgcag catttcgcct cccctgtcaa agatttgcaa catctcaacc tcatgattac cgcgggcccg acccgtgagc cattggatcc ggtgcgttat atcaccaacc acagctctgg caagatgggc ttcgcgattg ccgccgccgc agcgcagcgg ggcgcgaacg tgaccttgat cagcgggccg gtatcgctac cgaccccgcc gtttgtgcag cgcattgatg tcactaccgc gctggagatg gaagcggccg tccaggctgg cgcccagcag cagcacattt ttattggctg cgccgcagta gcggattacc gcgccgccgt catcgccgaa gataaaataa aaaaacaagg tgatgaatta acaataaaaa tggttaaaaa cccggatata gtggccgggg tcgcggcgct aaaatcacat cgcccctacg tcgttgggtt tgccgccgaa acgaattatg tggaagaata tgcgcggcaa aaacgtgccc gcaagaacct cgacctgatt tgcgccaacg atgtttccca gcctaatcag ggctttaaca gcgacagcaa cgcactacac cttttctggc aggatgggga aaagcgctta ccacttgagc gcaaggaact cctgggccaa ttattactcg acgagatcgt gacccgttat gatgaaaaaa atcgacgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat