KlpnC.01229.a
Orotidine 5'-phosphate decarboxylase
| CENTER ID: | KlpnC.01229.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.01229.a.B1.GE44296 | Full length(KlpnC.01229.a) | 1 | 228 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
8CSO
DEPOSITED: 5/13/2022
DETERMINATION: XRay
CLONE: KlpnC.01229.a.B1.GE44296
PROTEIN: KlpnC.01229.a.B1.PW39025
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.2129 |
| UniProt: | A0A0H3GNE4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVALDYDNRD KALAFVDRID PRDCRLKVGK EMFTLLGPQF VRDLHQRGFE VFLDLKFHDI PNTTARAVAA AAELGVWMVN VHASGGARMM TAAREALLPF GKDAPLLIAV TVLTSMESSD LQDLGITLSP ADYAAKLAAL TQRCGLDGVV CSAQEAVRFK HELGQAFKLV TPGIRPQGSD AGDQRRIMTP EQAQEAGVDY MVIGRPVTQS ADPAATLRAI NDSLRKGA
NT Sequence
GTGGTTGCGC TCGACTATGA TAATCGCGAT AAAGCGCTGG CATTTGTCGA CCGCATCGAC CCGCGCGACT GCCGCCTGAA GGTGGGCAAA GAGATGTTCA CCCTGCTGGG CCCGCAGTTT GTCCGCGATC TGCATCAGCG TGGGTTTGAG GTGTTCCTGG ACCTTAAGTT TCACGATATT CCCAACACCA CCGCCCGCGC GGTGGCCGCA GCGGCTGAGC TGGGCGTATG GATGGTGAAT GTCCATGCTT CCGGCGGCGC GCGAATGATG ACCGCCGCGC GCGAAGCGCT GCTGCCGTTT GGCAAAGATG CGCCGCTGCT GATCGCTGTC ACGGTGCTCA CCAGCATGGA GTCCAGCGAT CTACAGGATC TGGGCATTAC CTTATCTCCG GCTGACTACG CGGCGAAGCT GGCGGCGCTG ACCCAACGCT GCGGCCTTGA TGGGGTGGTG TGTTCAGCCC AGGAGGCTGT GCGCTTTAAG CATGAGCTGG GGCAGGCGTT TAAGCTGGTG ACCCCGGGGA TTCGTCCGCA GGGCAGCGAC GCTGGCGATC AGCGGCGAAT TATGACTCCT GAGCAGGCTC AGGAAGCTGG GGTAGACTAT ATGGTGATTG GTCGCCCGGT TACTCAGTCG GCGGATCCAG CCGCGACGCT GCGGGCGATC AACGATTCTC TGCGCAAGGG GGCA
Details for KlpnC.01229.a.B1.GE44296
| HARVESTED ON: | 5/21/2021 |
| SEQUENCED ON: | 6/4/2021 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV VALDYDNRDK ALAFVDRIDP RDCRLKVGKE MFTLLGPQFV RDLHQRGFEV FLDLKFHDIP NTTARAVAAA AELGVWMVNV HASGGARMMT AAREALLPFG KDAPLLIAVT VLTSMESSDL QDLGITLSPA DYAAKLAALT QRCGLDGVVC SAQEAVRFKH ELGQAFKLVT PGIRPQGSDA GDQRRIMTPE QAXEAGVDYM VIGRPVTQSA DPAATLRAIN DSLRKGHK
Validated NT Sequence
ggnnnnngnn nnnnnnnnna nnnnnactcn ctatagggag accacaacgg tttccctcta gaaataattt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatggtggtt gcgctcgact atgataatcg cgataaagcg ctggcatttg tcgaccgcat cgacccgcgc gactgccgcc tgaaggtggg caaagagatg ttcaccctgc tgggcccgca gtttgtccgc gatctgcatc agcgtgggtt tgaggtgttc ctggacctta agtttcacga tattcccaac accaccgccc gcgcggtggc cgcagcggct gagctgggcg tatggatggt gaatgtccat gcttccggcg gcgcgcgaat gatgaccgcc gcgcgcgaag cgctgctgcc gtttggcaaa gatgcgccgt tgctgatcgc tgtcacggtg ctcaccagca tggagtccag cgatctacag gatctgggca ttaccttatc tccggctgac tacgcggcga agctggcggc gctgactcaa cgctgcggcc ttgatggggt ggtgtgttca gcccaggagg ctgtgcgctt taagcatgag ctggggcagg cgtttaagct ggtgaccccg gggattcgtc cgcagggcag cgacgctggc gatcagcggc gaattatgac tcctgagcag gctcangaag ctggggtaga ctatatggtg attggtcgcc cggttactca gtcggcggat ccagccgcga cgctgcgggc gatcaacgat tctctgcgca aggggcataa gtgagtaaga tangatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc cnccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ngaggaacta tatcccggat atccacngga cgggtgtggt cngncatgat cgcgtagtcg atnntgggct ccaagtagcg aagcgagcan antgggcggc ggncaancgg tcgganagng ctccganaac ggnngncccn tagaaattgc nncaacncnn ttagcgctag cannncnccn tantgaactg gnnannctnt
Expected Protein Sequence
MAHHHHHHMV ALDYDNRDKA LAFVDRIDPR DCRLKVGKEM FTLLGPQFVR DLHQRGFEVF LDLKFHDIPN TTARAVAAAA ELGVWMVNVH ASGGARMMTA AREALLPFGK DAPLLIAVTV LTSMESSDLQ DLGITLSPAD YAAKLAALTQ RCGLDGVVCS AQEAVRFKHE LGQAFKLVTP GIRPQGSDAG DQRRIMTPEQ AQEAGVDYMV IGRPVTQSAD PAATLRAIND SLRKGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggttg cgctcgacta tgataatcgc gataaagcgc tggcatttgt cgaccgcatc gacccgcgcg actgccgcct gaaggtgggc aaagagatgt tcaccctgct gggcccgcag tttgtccgcg atctgcatca gcgtgggttt gaggtgttcc tggaccttaa gtttcacgat attcccaaca ccaccgcccg cgcggtggcc gcagcggctg agctgggcgt atggatggtg aatgtccatg cttccggcgg cgcgcgaatg atgaccgccg cgcgcgaagc gctgctgccg tttggcaaag atgcgccgct gctgatcgct gtcacggtgc tcaccagcat ggagtccagc gatctacagg atctgggcat taccttatct ccggctgact acgcggcgaa gctggcggcg ctgacccaac gctgcggcct tgatggggtg gtgtgttcag cccaggaggc tgtgcgcttt aagcatgagc tggggcaggc gtttaagctg gtgaccccgg ggattcgtcc gcagggcagc gacgctggcg atcagcggcg aattatgact cctgagcagg ctcaggaagc tggggtagac tatatggtga ttggtcgccc ggttactcag tcggcggatc cagccgcgac gctgcgggcg atcaacgatt ctctgcgcaa gggggcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at