KlpnC.01187.a

Glutamate--tRNA ligase

CENTER ID: KlpnC.01187.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.01187.a.B1.GE44295 Full length(KlpnC.01187.a) 1 472
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.01187.a.B1.PW39024 Full length(KlpnC.01187.a) 1 472

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3723
UniProt: A0A0H3GTA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIKTRFAPS PTGYLHVGGA RTALYSWLFA RNHGGEFVLR IEDTDLERST PEAIEAIMDG MNWLNLQWDE GPYFQTKRFD RYNNVIDEML EAGTAYKCYC SKERLEALRE EQMAKGEKPR YDGRCRHSHE HHADDEPCVV RFANPQEGSV IFDDQIRGPI EFSNQELDDL IIRRTDGSPT YNFCVVVDDW DMAITHVIRG EDHINNTPRQ INILKALNAP VPVYAHVSMI NGDDGKKLSK RHGAVSVMQY RDDGYLPEAL LNYLVRLGWS HGDQEIFTRE EMIEFFSLGA VSKSASAFNT DKLLWLNHHY INTLPAEYVA THLQWHIEQE NIDTRNGPQL AELVKLLGER CKTLKEMAQS CRYFYEEFAE FDADAAKKHL RPVARQPLEV VRDKLAAISD WTAENVHHAI QATADELEVG MGKVGMPLRV AVTGAGQSPA LDVTVHAIGK SRSVDRINKA LAFIAEREGP AS
NT Sequence
ATGAAAATCA AAACTCGCTT CGCGCCAAGC CCAACCGGCT ATCTGCACGT TGGCGGTGCG CGTACTGCTC TCTATTCCTG GCTTTTTGCC CGTAACCACG GCGGTGAGTT TGTGCTGCGT ATTGAAGACA CCGATCTCGA ACGCTCCACC CCGGAAGCAA TTGAAGCCAT CATGGATGGC ATGAACTGGC TGAATCTGCA ATGGGACGAA GGCCCCTATT TCCAGACCAA ACGTTTCGAT CGTTATAACA ACGTAATCGA TGAGATGCTG GAAGCCGGTA CCGCTTACAA ATGCTATTGC TCGAAAGAAC GTCTGGAAGC GCTGCGCGAA GAGCAGATGG CGAAGGGTGA GAAGCCGCGT TACGATGGCC GCTGCCGCCA TAGCCACGAG CACCATGCTG ACGATGAGCC GTGCGTCGTG CGTTTCGCCA ACCCGCAGGA AGGTTCGGTT ATTTTTGACG ATCAGATCCG CGGACCTATC GAGTTCAGCA ACCAGGAACT GGACGATCTG ATCATTCGCC GTACCGATGG TTCCCCGACC TATAACTTCT GCGTGGTGGT AGATGACTGG GATATGGCGA TCACTCACGT GATCCGCGGC GAAGATCATA TCAACAACAC GCCGCGCCAG ATCAACATCC TCAAAGCGCT GAATGCGCCG GTGCCTGTTT ATGCGCACGT GTCGATGATC AACGGCGATG ACGGTAAAAA ACTTTCCAAG CGCCACGGCG CAGTGAGCGT GATGCAGTAT CGCGACGACG GCTACCTGCC GGAAGCGCTA CTCAACTATC TGGTGCGCCT GGGCTGGTCT CATGGCGATC AGGAGATTTT CACTCGCGAA GAGATGATTG AGTTCTTCTC GCTGGGCGCG GTGAGCAAAT CCGCCAGTGC CTTTAACACC GATAAACTGC TGTGGTTAAA CCATCACTAT ATCAATACCC TGCCGGCGGA GTATGTGGCA ACGCATCTGC AGTGGCATAT CGAGCAGGAA AATATCGACA CTCGCAACGG CCCGCAGCTG GCTGAGCTGG TGAAACTGCT GGGCGAGCGT TGCAAGACGC TGAAAGAGAT GGCCCAGAGC TGCCGCTACT TCTATGAAGA GTTCGCTGAA TTCGATGCCG ACGCCGCGAA AAAACATCTG CGTCCAGTGG CGCGTCAGCC GCTGGAAGTG GTTCGCGATA AGCTGGCGGC GATTAGCGAC TGGACGGCGG AAAACGTCCA TCACGCGATT CAGGCAACTG CTGATGAGCT GGAGGTGGGG ATGGGTAAAG TCGGTATGCC GCTGCGCGTG GCGGTTACCG GGGCAGGGCA ATCTCCTGCG CTAGACGTTA CTGTCCATGC TATCGGTAAA TCCCGTAGCG TAGATCGTAT CAATAAAGCG CTGGCTTTTA TCGCTGAACG CGAAGGCCCG GCGTCC
Details for KlpnC.01187.a.B1.GE44295
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 55kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 60
Validated AA Sequence
MAHHHHHHMK IKTRFAPSPT GYLHVGGART ALYSWLFARN HGGEFVLRIE DTDLERSTPE AIEAIMDGMN WLNLQWDEGP YFQTKRFDRY NNVIDEMLEA GTAYKCYCSK ERLEALREEQ MAKGEKPRYD GRCRHSHEHH ADDEPCVVRF ANPQQGSVIF DDQIRGPIEF SNQELDDLII RRTDGSPTYN FCVVVDDWDM AITHVIRGED HINNTPRQIN ILKALNAPVP VYAHVSMING DDGKKLSKRH GAVSVMQYRD DGYLPEALLN YLVRLGWSHG DXEXFTPKR
Validated NT Sequence
acgactcnct atagggagac cacaangnnn tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tgaaaatcaa aactcgcttc gcgccaagcc caaccggcta tctgcacgtt ggcggtgcgc gtactgctct ctattcctgg ctttttgccc gtaaccacgg cggtgagttt gtgctgcgta ttgaagacac cgatctcgaa cgctccaccc cggaagcaat tgaagccatc atggatggca tgaactggct gaatctgcaa tgggacgaag gcccctattt ccagaccaaa cgtttcgatc gttataacaa cgtaatcgat gagatgctgg aagccggtac cgcttacaaa tgctattgct cgaaagaacg tctggaagcg ctgcgcgaag agcagatggc gaagggtgag aagccgcgtt acgatggccg ctgccgccat agccacgagc accatgctga cgatgagccg tgcgtcgtgc gtttcgccaa cccgcagcaa ggttcggtta tttttgacga tcagatccgc ggacctatcg agttcagcaa ccaggaactg gacgatctga tcattcgccg taccgatggt tccccgacct ataacttctg cgtggtggta gatgactggg atatggcgat cactcacgtg atccgcggcg aagatcatat caacaacacg ccgcgccaga tcaatatcct caaagcgctg aatgcgccgg tgccggttta tgcgcacgtg tcgatgatca acggcgatga cggtaaaaaa ctttccaagc gccacggcgc agtgagcgtg atgcagtatc gcgacgacgg ctacctgccg gaagcgctac tcaactatct ggtgcgcctg ggctggtctc atggcgatcn ngagantttc actccgaaga gatgattgag ntncttctcg ctgggcgcgg tgancaannn cgccagtgcc tttaacancg ataaactgct gnggttaaat catccctann atcaataccc tgccggcggn annatgnngn nacgcattnn gcagnggnnn tantcgang
Expected Protein Sequence
MAHHHHHHMK IKTRFAPSPT GYLHVGGART ALYSWLFARN HGGEFVLRIE DTDLERSTPE AIEAIMDGMN WLNLQWDEGP YFQTKRFDRY NNVIDEMLEA GTAYKCYCSK ERLEALREEQ MAKGEKPRYD GRCRHSHEHH ADDEPCVVRF ANPQEGSVIF DDQIRGPIEF SNQELDDLII RRTDGSPTYN FCVVVDDWDM AITHVIRGED HINNTPRQIN ILKALNAPVP VYAHVSMING DDGKKLSKRH GAVSVMQYRD DGYLPEALLN YLVRLGWSHG DQEIFTREEM IEFFSLGAVS KSASAFNTDK LLWLNHHYIN TLPAEYVATH LQWHIEQENI DTRNGPQLAE LVKLLGERCK TLKEMAQSCR YFYEEFAEFD ADAAKKHLRP VARQPLEVVR DKLAAISDWT AENVHHAIQA TADELEVGMG KVGMPLRVAV TGAGQSPALD VTVHAIGKSR SVDRINKALA FIAEREGPAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tcaaaactcg cttcgcgcca agcccaaccg gctatctgca cgttggcggt gcgcgtactg ctctctattc ctggcttttt gcccgtaacc acggcggtga gtttgtgctg cgtattgaag acaccgatct cgaacgctcc accccggaag caattgaagc catcatggat ggcatgaact ggctgaatct gcaatgggac gaaggcccct atttccagac caaacgtttc gatcgttata acaacgtaat cgatgagatg ctggaagccg gtaccgctta caaatgctat tgctcgaaag aacgtctgga agcgctgcgc gaagagcaga tggcgaaggg tgagaagccg cgttacgatg gccgctgccg ccatagccac gagcaccatg ctgacgatga gccgtgcgtc gtgcgtttcg ccaacccgca ggaaggttcg gttatttttg acgatcagat ccgcggacct atcgagttca gcaaccagga actggacgat ctgatcattc gccgtaccga tggttccccg acctataact tctgcgtggt ggtagatgac tgggatatgg cgatcactca cgtgatccgc ggcgaagatc atatcaacaa cacgccgcgc cagatcaaca tcctcaaagc gctgaatgcg ccggtgcctg tttatgcgca cgtgtcgatg atcaacggcg atgacggtaa aaaactttcc aagcgccacg gcgcagtgag cgtgatgcag tatcgcgacg acggctacct gccggaagcg ctactcaact atctggtgcg cctgggctgg tctcatggcg atcaggagat tttcactcgc gaagagatga ttgagttctt ctcgctgggc gcggtgagca aatccgccag tgcctttaac accgataaac tgctgtggtt aaaccatcac tatatcaata ccctgccggc ggagtatgtg gcaacgcatc tgcagtggca tatcgagcag gaaaatatcg acactcgcaa cggcccgcag ctggctgagc tggtgaaact gctgggcgag cgttgcaaga cgctgaaaga gatggcccag agctgccgct acttctatga agagttcgct gaattcgatg ccgacgccgc gaaaaaacat ctgcgtccag tggcgcgtca gccgctggaa gtggttcgcg ataagctggc ggcgattagc gactggacgg cggaaaacgt ccatcacgcg attcaggcaa ctgctgatga gctggaggtg gggatgggta aagtcggtat gccgctgcgc gtggcggtta ccggggcagg gcaatctcct gcgctagacg ttactgtcca tgctatcggt aaatcccgta gcgtagatcg tatcaataaa gcgctggctt ttatcgctga acgcgaaggc ccggcgtcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for KlpnC.01187.a.B1.PW39024
PURIFICATION DATe: 6/15/2021
CONCENTRATION: 15mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 60
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK IKTRFAPSPT GYLHVGGART ALYSWLFARN HGGEFVLRIE DTDLERSTPE AIEAIMDGMN WLNLQWDEGP YFQTKRFDRY NNVIDEMLEA GTAYKCYCSK ERLEALREEQ MAKGEKPRYD GRCRHSHEHH ADDEPCVVRF ANPQQGSVIF DDQIRGPIEF SNQELDDLII RRTDGSPTYN FCVVVDDWDM AITHVIRGED HINNTPRQIN ILKALNAPVP VYAHVSMING DDGKKLSKRH GAVSVMQYRD DGYLPEALLN YLVRLGWSHG DXEXFTPKR
Validated NT Sequence
acgactcnct atagggagac cacaangnnn tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tgaaaatcaa aactcgcttc gcgccaagcc caaccggcta tctgcacgtt ggcggtgcgc gtactgctct ctattcctgg ctttttgccc gtaaccacgg cggtgagttt gtgctgcgta ttgaagacac cgatctcgaa cgctccaccc cggaagcaat tgaagccatc atggatggca tgaactggct gaatctgcaa tgggacgaag gcccctattt ccagaccaaa cgtttcgatc gttataacaa cgtaatcgat gagatgctgg aagccggtac cgcttacaaa tgctattgct cgaaagaacg tctggaagcg ctgcgcgaag agcagatggc gaagggtgag aagccgcgtt acgatggccg ctgccgccat agccacgagc accatgctga cgatgagccg tgcgtcgtgc gtttcgccaa cccgcagcaa ggttcggtta tttttgacga tcagatccgc ggacctatcg agttcagcaa ccaggaactg gacgatctga tcattcgccg taccgatggt tccccgacct ataacttctg cgtggtggta gatgactggg atatggcgat cactcacgtg atccgcggcg aagatcatat caacaacacg ccgcgccaga tcaatatcct caaagcgctg aatgcgccgg tgccggttta tgcgcacgtg tcgatgatca acggcgatga cggtaaaaaa ctttccaagc gccacggcgc agtgagcgtg atgcagtatc gcgacgacgg ctacctgccg gaagcgctac tcaactatct ggtgcgcctg ggctggtctc atggcgatcn ngagantttc actccgaaga gatgattgag ntncttctcg ctgggcgcgg tgancaannn cgccagtgcc tttaacancg ataaactgct gnggttaaat catccctann atcaataccc tgccggcggn annatgnngn nacgcattnn gcagnggnnn tantcgang
Expressed Protein Sequence
MAHHHHHHMK IKTRFAPSPT GYLHVGGART ALYSWLFARN HGGEFVLRIE DTDLERSTPE AIEAIMDGMN WLNLQWDEGP YFQTKRFDRY NNVIDEMLEA GTAYKCYCSK ERLEALREEQ MAKGEKPRYD GRCRHSHEHH ADDEPCVVRF ANPQEGSVIF DDQIRGPIEF SNQELDDLII RRTDGSPTYN FCVVVDDWDM AITHVIRGED HINNTPRQIN ILKALNAPVP VYAHVSMING DDGKKLSKRH GAVSVMQYRD DGYLPEALLN YLVRLGWSHG DQEIFTREEM IEFFSLGAVS KSASAFNTDK LLWLNHHYIN TLPAEYVATH LQWHIEQENI DTRNGPQLAE LVKLLGERCK TLKEMAQSCR YFYEEFAEFD ADAAKKHLRP VARQPLEVVR DKLAAISDWT AENVHHAIQA TADELEVGMG KVGMPLRVAV TGAGQSPALD VTVHAIGKSR SVDRINKALA FIAEREGPAS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA TCAAAACTCG CTTCGCGCCA AGCCCAACCG GCTATCTGCA CGTTGGCGGT GCGCGTACTG CTCTCTATTC CTGGCTTTTT GCCCGTAACC ACGGCGGTGA GTTTGTGCTG CGTATTGAAG ACACCGATCT CGAACGCTCC ACCCCGGAAG CAATTGAAGC CATCATGGAT GGCATGAACT GGCTGAATCT GCAATGGGAC GAAGGCCCCT ATTTCCAGAC CAAACGTTTC GATCGTTATA ACAACGTAAT CGATGAGATG CTGGAAGCCG GTACCGCTTA CAAATGCTAT TGCTCGAAAG AACGTCTGGA AGCGCTGCGC GAAGAGCAGA TGGCGAAGGG TGAGAAGCCG CGTTACGATG GCCGCTGCCG CCATAGCCAC GAGCACCATG CTGACGATGA GCCGTGCGTC GTGCGTTTCG CCAACCCGCA GGAAGGTTCG GTTATTTTTG ACGATCAGAT CCGCGGACCT ATCGAGTTCA GCAACCAGGA ACTGGACGAT CTGATCATTC GCCGTACCGA TGGTTCCCCG ACCTATAACT TCTGCGTGGT GGTAGATGAC TGGGATATGG CGATCACTCA CGTGATCCGC GGCGAAGATC ATATCAACAA CACGCCGCGC CAGATCAACA TCCTCAAAGC GCTGAATGCG CCGGTGCCTG TTTATGCGCA CGTGTCGATG ATCAACGGCG ATGACGGTAA AAAACTTTCC AAGCGCCACG GCGCAGTGAG CGTGATGCAG TATCGCGACG ACGGCTACCT GCCGGAAGCG CTACTCAACT ATCTGGTGCG CCTGGGCTGG TCTCATGGCG ATCAGGAGAT TTTCACTCGC GAAGAGATGA TTGAGTTCTT CTCGCTGGGC GCGGTGAGCA AATCCGCCAG TGCCTTTAAC ACCGATAAAC TGCTGTGGTT AAACCATCAC TATATCAATA CCCTGCCGGC GGAGTATGTG GCAACGCATC TGCAGTGGCA TATCGAGCAG GAAAATATCG ACACTCGCAA CGGCCCGCAG CTGGCTGAGC TGGTGAAACT GCTGGGCGAG CGTTGCAAGA CGCTGAAAGA GATGGCCCAG AGCTGCCGCT ACTTCTATGA AGAGTTCGCT GAATTCGATG CCGACGCCGC GAAAAAACAT CTGCGTCCAG TGGCGCGTCA GCCGCTGGAA GTGGTTCGCG ATAAGCTGGC GGCGATTAGC GACTGGACGG CGGAAAACGT CCATCACGCG ATTCAGGCAA CTGCTGATGA GCTGGAGGTG GGGATGGGTA AAGTCGGTAT GCCGCTGCGC GTGGCGGTTA CCGGGGCAGG GCAATCTCCT GCGCTAGACG TTACTGTCCA TGCTATCGGT AAATCCCGTA GCGTAGATCG TATCAATAAA GCGCTGGCTT TTATCGCTGA ACGCGAAGGC CCGGCGTCCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT