KlpnC.01141.a

Ribokinase

CENTER ID: KlpnC.01141.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.01141.a.B2.GE44294 Mature protein(KlpnC.01141.a) 16 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.11
UniProt: A0A0H3GPH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMKAAGKLVV LGSINADHIL NLDAFPTPGE TVTGHHYQVA FGGKGANQAV AAGRSGADIA FIACTGDDDI GERIRRQLAS DKIDVAPVRA VAGEATGVAL IFVNAEGENV IGIHAGANAA LSVSQVEAEK ERIASAQALL MQLESPLESV IAAAKIAHHH HTTVVLNPAP ARELPDELLA LVDIITPNET EAEKLTGIRV ESDEDAAKAA NVLHAKGIGT VMITLGSRGV WLSAEGESRR ILGFRVQAID TIAAGDTFNG ALVTALLEGT ALPEAIRFAH AAAAIAVTRK GAQPSVPWRT EIDEFLAQQG
NT Sequence
ATGATGAAAG CTGCAGGCAA ACTTGTCGTC CTTGGCAGTA TCAATGCCGA TCACATTCTT AACCTTGACG CTTTCCCGAC GCCGGGCGAA ACCGTCACCG GCCACCATTA TCAGGTGGCG TTCGGTGGAA AAGGGGCCAA CCAGGCGGTG GCCGCCGGGC GCAGCGGAGC GGATATCGCG TTTATCGCCT GCACCGGCGA TGACGATATC GGTGAACGCA TCCGTCGTCA GCTGGCGAGC GATAAAATCG ACGTGGCCCC CGTGCGCGCG GTTGCCGGGG AAGCGACCGG GGTGGCGTTA ATTTTCGTTA ACGCCGAAGG TGAGAATGTC ATCGGTATTC ATGCCGGCGC CAATGCGGCG CTTTCCGTCT CCCAGGTTGA GGCGGAAAAA GAGCGTATCG CCAGCGCACA GGCGTTGTTG ATGCAGCTGG AGTCGCCGCT GGAGAGCGTC ATAGCGGCGG CGAAAATCGC CCATCACCAT CACACCACCG TGGTGCTGAA CCCCGCGCCA GCGCGTGAAC TACCGGATGA ACTGCTGGCG CTGGTGGATA TTATTACCCC TAACGAAACC GAAGCGGAAA AGCTGACCGG TATTCGGGTT GAGAGTGACG AAGATGCGGC AAAAGCCGCC AACGTGCTGC ATGCCAAAGG CATTGGTACG GTGATGATCA CCCTCGGCAG CCGCGGCGTC TGGCTCAGTG CCGAAGGCGA AAGCCGCCGG ATCCTAGGGT TTCGCGTACA GGCCATTGAT ACTATCGCGG CCGGTGATAC CTTTAACGGC GCGCTGGTGA CCGCGCTGCT GGAAGGGACC GCCCTGCCGG AGGCGATTCG CTTTGCGCAT GCCGCAGCGG CGATCGCCGT AACCCGCAAA GGTGCTCAGC CGTCCGTGCC TTGGCGCACA GAGATCGATG AATTTTTAGC GCAACAGGGG
Details for KlpnC.01141.a.B2.GE44294
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 93
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMA DHILNLDAFP TPGETVTGHH YQVAFGGKGA NQAVAAGRSG ADIAFIACTG DDDIGERIRR QLASDKIDVA PVRAVAREAT GVALIFVNAE GENVIGIHAG ANAALSVSQV EAEKERIASA QALLMQLESP LESVIAAAKI AHHHHTTVVL NPAPARELPD ELLALVDIIT PNETEAEKLT GIRVESDEDA AKAANVLHAK GIGTVMITLG SRGVWLSAEX ESRRIPGFRV QAIDTIAAGD XFNGALVTAL LGRDRPAGGX SLCACRSGDR P
Validated NT Sequence
cnactnntnt agggagacca caacggtttc cctctagaaa taattttgtt taactttang aaggagatat accatggctc accaccacca ccaccatatg gccgatcaca ttcttaacct tgacgctttc ccgacgccgg gcgaaaccgt caccggccac cattatcagg tggcgttcgg tggaaaaggg gccaaccagg cggtggccgc cgggcgcagc ggagcggata tcgcgtttat cgcctgcacc ggcgatgacg atatcggtga acgcatccgt cgtcagctgg cgagcgataa aatcgacgtg gcgcccgtgc gcgcggttgc cagggaagcg accggggtgg cgttaatttt cgttaacgcc gaaggtgaga atgtcatcgg tattcatgcc ggcgccaatg cggcgctttc cgtctcccag gttgaggcgg aaaaagagcg tatcgccagc gcacaggcgt tgttgatgca gctggagtcg ccgctggaga gcgtcatagc ggcggcgaaa atcgcccatc accatcacac caccgtggtg ctgaaccccg cgccagcgcg tgaactaccg gatgaactgc tggcgctggt ggatattatt acccctaacg aaaccgaagc ggaaaagctg accggtattc gcgttgagag tgacgaagat gcggcaaaag ccgccaacgt gctgcatgcc aaaggcattg gtacggtgat gatcaccctc ggcagccgcg gcgtctggct cagtgccgaa ngcgaaagcc gccggatccc agggtttcgc gtacaggcca ttgatactat cgcggccggt gatnccttta acggcgcgct ggtgaccgcg ctgctgggaa gggaccgccc tgccggaggc gantcgcttt gcgcatgccg cagcggcgat cgcccgtaac ccgcaaaggc gctcanccgt ccgngccttg gcgcacagag atcgatgnan ttttnancgc annngggtan ngagnannat aggattcggg ctgctancaa anccnaangg agctgantng gctgcntgcc nnnnnnnnnn nnaataanct a
Expected Protein Sequence
MAHHHHHHAD HILNLDAFPT PGETVTGHHY QVAFGGKGAN QAVAAGRSGA DIAFIACTGD DDIGERIRRQ LASDKIDVAP VRAVAGEATG VALIFVNAEG ENVIGIHAGA NAALSVSQVE AEKERIASAQ ALLMQLESPL ESVIAAAKIA HHHHTTVVLN PAPARELPDE LLALVDIITP NETEAEKLTG IRVESDEDAA KAANVLHAKG IGTVMITLGS RGVWLSAEGE SRRILGFRVQ AIDTIAAGDT FNGALVTALL EGTALPEAIR FAHAAAAIAV TRKGAQPSVP WRTEIDEFLA QQG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgatc acattcttaa ccttgacgct ttcccgacgc cgggcgaaac cgtcaccggc caccattatc aggtggcgtt cggtggaaaa ggggccaacc aggcggtggc cgccgggcgc agcggagcgg atatcgcgtt tatcgcctgc accggcgatg acgatatcgg tgaacgcatc cgtcgtcagc tggcgagcga taaaatcgac gtggcccccg tgcgcgcggt tgccggggaa gcgaccgggg tggcgttaat tttcgttaac gccgaaggtg agaatgtcat cggtattcat gccggcgcca atgcggcgct ttccgtctcc caggttgagg cggaaaaaga gcgtatcgcc agcgcacagg cgttgttgat gcagctggag tcgccgctgg agagcgtcat agcggcggcg aaaatcgccc atcaccatca caccaccgtg gtgctgaacc ccgcgccagc gcgtgaacta ccggatgaac tgctggcgct ggtggatatt attaccccta acgaaaccga agcggaaaag ctgaccggta ttcgggttga gagtgacgaa gatgcggcaa aagccgccaa cgtgctgcat gccaaaggca ttggtacggt gatgatcacc ctcggcagcc gcggcgtctg gctcagtgcc gaaggcgaaa gccgccggat cctagggttt cgcgtacagg ccattgatac tatcgcggcc ggtgatacct ttaacggcgc gctggtgacc gcgctgctgg aagggaccgc cctgccggag gcgattcgct ttgcgcatgc cgcagcggcg atcgccgtaa cccgcaaagg tgctcagccg tccgtgcctt ggcgcacaga gatcgatgaa tttttagcgc aacaggggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat