SSGCID: Target Page

KlpnC.01136.a

1-deoxy-D-xylulose 5-phosphate reductoisomerase

CENTER ID:	KlpnC.01136.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.01136.a.B1.GE44293	Full length(KlpnC.01136.a)		1	400

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.01136.a.B1.PW39022	Full length(KlpnC.01136.a)		1	400

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.892
UniProt:	A0A0H3GJN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKQLTVLGST GSIGCSTLDV VRHNPGRFSV AALVAGKNVD RMVEQCLEFT PRYAVMDDAQ SAERLRTRLH EHGSRTEVLS GQQAAAEVAA LDEVDQVMAA IVGAAGLVPT LAAIRAGKTV LLANKESLVT CGRLFMEAVQ QSGARLLPVD SEHNAIFQSM PETIQQHLGY ADLARNGVSS ILLTGSGGPF RETAVAELAA MTPDQACRHP NWSMGRKISV DSATMMNKGL EYIEARWLFN ASAQQMEVLI HPQSVIHSMV RYQDGSVLAQ LGEPDMRTPI AHTMGWPQRL NSGVKPLDFC QLSNLSFSAP DYTRYPCLKL AMDAFDVGQA ATTTLNAANE ESVAAFLHGD IRFTDIAAVN LAVLDKMDLQ EPQSIDDVLV IDAEARAIAH QQLQRLVAQA

NT Sequence

ATGAAGCAAT TAACCGTTCT CGGTTCGACC GGCTCCATTG GCTGTAGCAC GCTGGACGTG GTGCGCCATA ACCCTGGTCG CTTTTCCGTC GCCGCGCTGG TCGCGGGGAA AAACGTTGAC CGTATGGTTG AACAATGCCT GGAATTTACC CCCCGTTACG CGGTGATGGA CGATGCGCAA AGCGCTGAGC GGTTGCGCAC CCGTCTGCAT GAGCATGGCA GTCGCACCGA GGTGCTGAGC GGCCAGCAGG CGGCGGCAGA GGTCGCGGCG CTGGATGAGG TCGATCAGGT CATGGCGGCA ATCGTCGGCG CGGCGGGACT GGTGCCGACG CTGGCGGCGA TCCGCGCCGG GAAAACGGTA TTGTTAGCGA ATAAAGAATC GCTGGTGACC TGCGGTCGCT TATTCATGGA GGCGGTGCAA CAGTCGGGGG CGCGATTGCT ACCGGTCGAT AGCGAGCATA ACGCTATTTT TCAGAGTATG CCGGAGACAA TTCAGCAGCA TCTGGGATAC GCTGATCTTG CCCGGAATGG CGTCTCGTCA ATCCTGCTGA CCGGCTCCGG TGGGCCTTTC CGCGAGACGG CCGTGGCTGA ACTGGCGGCG ATGACCCCGG ATCAGGCCTG CCGTCATCCC AACTGGTCGA TGGGGCGCAA GATCTCCGTC GATTCGGCCA CGATGATGAA CAAAGGCCTG GAATATATTG AAGCGCGCTG GCTGTTTAAC GCCTCGGCCC AGCAGATGGA AGTGCTGATC CATCCGCAGT CGGTGATCCA CTCGATGGTT CGCTATCAGG ATGGCAGCGT GCTGGCCCAG CTTGGGGAGC CGGATATGCG CACGCCGATC GCCCATACCA TGGGCTGGCC GCAGCGACTG AATTCCGGCG TTAAACCGCT GGACTTCTGT CAGTTGAGCA ATCTGAGCTT CTCCGCGCCG GACTATACGC GATATCCGTG CCTGAAGCTG GCGATGGACG CGTTTGACGT CGGCCAGGCC GCGACCACGA CGCTGAATGC GGCTAATGAA GAGTCGGTTG CCGCGTTTCT GCATGGCGAT ATCCGCTTTA CCGATATTGC GGCGGTTAAC CTGGCGGTGC TGGATAAGAT GGACCTGCAG GAGCCGCAAA GCATTGATGA TGTTCTGGTT ATCGACGCCG AGGCGCGCGC AATCGCTCAC CAGCAGCTGC AGCGGTTGGT TGCGCAGGCG

Details for KlpnC.01136.a.B1.GE44293

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	64

Validated AA Sequence

MEAVQQSGAR LLPVDSEHNA IFQSMPETIQ QHLGYADLAQ NGVSSILLTG SGGPFRETAV AELAAMTPDQ ACRHPNWSMG RKISVDSATM MNKGLEYIEA RWLFNASAQQ MEVLIHPQSV IHSMVRYQDG SVLAQLGEPD MRTPIAHTMG WPQRLNSGVK PLDFCQLSNL SFSAPDYTRY PCLKLAMDAF DVGQAATTTL NAANEESVAA FLHGDIRFTD IAAVNLAVLD KMDLQEPQSI DDVLVIDAEA RAIAHQQLQR LVAQA

Validated NT Sequence

ggctttgtta gcagccggat cctatcttac tcacttacgc ctgcgcaacc aaccgctgca gctgctggtg agcgattgcg cgcgcctcgg cgtcgataac cagaacatca tcaatgcttt gcggctcctg caggtccatc ttatccagca ccgccaggtt aaccgccgca atatcggtaa agcggatatc gccatgcaga aacgcggcaa ccgactcttc attagccgca ttcagcgtcg tggtcgcggc ctggccgacg tcaaacgcgt ccatcgccag cttcaggcac ggatatcgcg tatagtccgg cgcggagaag ctcagattgc tcaactgaca gaagtccagc ggtttaacgc cggaattcag tcgctgcggc cagcccatgg tatgggcgat cggcgtgcgc atatccggct ccccaagctg ggccagcacg ctgccatcct gatagcgaac catcgagtgg atcaccgact gcggatggat cagcacttcc atctgctggg ccgaggcgtt aaacagccag cgcgcttcaa tatattccag gcctttgttc atcatcgtgg ccgaatcaac ggagatcttg cgccccatcg accagttggg atgacggcag gcctgatccg gggtcatcgc cgccagttca gccacggccg tctcgcggaa aggcccaccg gagccggtca gcaggataga cgagacgccg ttctgggcaa gatcagcgta tcccagatgc tgctgaattg tctccggcat actctgaaaa atagcgttat gctcgctatc gaccggtagc aatcgcgccc ccgactgttg caccgcctcc atgaataagc gaccgcaggt caccagcgat tctttattcg ctaacaatac cgttttcccg gcgcggatcg ccgccagcgt cggcaccagt cccgccgc

Expected Protein Sequence

MAHHHHHHMK QLTVLGSTGS IGCSTLDVVR HNPGRFSVAA LVAGKNVDRM VEQCLEFTPR YAVMDDAQSA ERLRTRLHEH GSRTEVLSGQ QAAAEVAALD EVDQVMAAIV GAAGLVPTLA AIRAGKTVLL ANKESLVTCG RLFMEAVQQS GARLLPVDSE HNAIFQSMPE TIQQHLGYAD LARNGVSSIL LTGSGGPFRE TAVAELAAMT PDQACRHPNW SMGRKISVDS ATMMNKGLEY IEARWLFNAS AQQMEVLIHP QSVIHSMVRY QDGSVLAQLG EPDMRTPIAH TMGWPQRLNS GVKPLDFCQL SNLSFSAPDY TRYPCLKLAM DAFDVGQAAT TTLNAANEES VAAFLHGDIR FTDIAAVNLA VLDKMDLQEP QSIDDVLVID AEARAIAHQQ LQRLVAQA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc aattaaccgt tctcggttcg accggctcca ttggctgtag cacgctggac gtggtgcgcc ataaccctgg tcgcttttcc gtcgccgcgc tggtcgcggg gaaaaacgtt gaccgtatgg ttgaacaatg cctggaattt accccccgtt acgcggtgat ggacgatgcg caaagcgctg agcggttgcg cacccgtctg catgagcatg gcagtcgcac cgaggtgctg agcggccagc aggcggcggc agaggtcgcg gcgctggatg aggtcgatca ggtcatggcg gcaatcgtcg gcgcggcggg actggtgccg acgctggcgg cgatccgcgc cgggaaaacg gtattgttag cgaataaaga atcgctggtg acctgcggtc gcttattcat ggaggcggtg caacagtcgg gggcgcgatt gctaccggtc gatagcgagc ataacgctat ttttcagagt atgccggaga caattcagca gcatctggga tacgctgatc ttgcccggaa tggcgtctcg tcaatcctgc tgaccggctc cggtgggcct ttccgcgaga cggccgtggc tgaactggcg gcgatgaccc cggatcaggc ctgccgtcat cccaactggt cgatggggcg caagatctcc gtcgattcgg ccacgatgat gaacaaaggc ctggaatata ttgaagcgcg ctggctgttt aacgcctcgg cccagcagat ggaagtgctg atccatccgc agtcggtgat ccactcgatg gttcgctatc aggatggcag cgtgctggcc cagcttgggg agccggatat gcgcacgccg atcgcccata ccatgggctg gccgcagcga ctgaattccg gcgttaaacc gctggacttc tgtcagttga gcaatctgag cttctccgcg ccggactata cgcgatatcc gtgcctgaag ctggcgatgg acgcgtttga cgtcggccag gccgcgacca cgacgctgaa tgcggctaat gaagagtcgg ttgccgcgtt tctgcatggc gatatccgct ttaccgatat tgcggcggtt aacctggcgg tgctggataa gatggacctg caggagccgc aaagcattga tgatgttctg gttatcgacg ccgaggcgcg cgcaatcgct caccagcagc tgcagcggtt ggttgcgcag gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for KlpnC.01136.a.B1.PW39022

PURIFICATION DATe:	6/8/2021
CONCENTRATION:	10mg/ml
OBSERVED MW:	44kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	64

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC AATTAACCGT TCTCGGTTCG ACCGGCTCCA TTGGCTGTAG CACGCTGGAC GTGGTGCGCC ATAACCCTGG TCGCTTTTCC GTCGCCGCGC TGGTCGCGGG GAAAAACGTT GACCGTATGG TTGAACAATG CCTGGAATTT ACCCCCCGTT ACGCGGTGAT GGACGATGCG CAAAGCGCTG AGCGGTTGCG CACCCGTCTG CATGAGCATG GCAGTCGCAC CGAGGTGCTG AGCGGCCAGC AGGCGGCGGC AGAGGTCGCG GCGCTGGATG AGGTCGATCA GGTCATGGCG GCAATCGTCG GCGCGGCGGG ACTGGTGCCG ACGCTGGCGG CGATCCGCGC CGGGAAAACG GTATTGTTAG CGAATAAAGA ATCGCTGGTG ACCTGCGGTC GCTTATTCAT GGAGGCGGTG CAACAGTCGG GGGCGCGATT GCTACCGGTC GATAGCGAGC ATAACGCTAT TTTTCAGAGT ATGCCGGAGA CAATTCAGCA GCATCTGGGA TACGCTGATC TTGCCCGGAA TGGCGTCTCG TCAATCCTGC TGACCGGCTC CGGTGGGCCT TTCCGCGAGA CGGCCGTGGC TGAACTGGCG GCGATGACCC CGGATCAGGC CTGCCGTCAT CCCAACTGGT CGATGGGGCG CAAGATCTCC GTCGATTCGG CCACGATGAT GAACAAAGGC CTGGAATATA TTGAAGCGCG CTGGCTGTTT AACGCCTCGG CCCAGCAGAT GGAAGTGCTG ATCCATCCGC AGTCGGTGAT CCACTCGATG GTTCGCTATC AGGATGGCAG CGTGCTGGCC CAGCTTGGGG AGCCGGATAT GCGCACGCCG ATCGCCCATA CCATGGGCTG GCCGCAGCGA CTGAATTCCG GCGTTAAACC GCTGGACTTC TGTCAGTTGA GCAATCTGAG CTTCTCCGCG CCGGACTATA CGCGATATCC GTGCCTGAAG CTGGCGATGG ACGCGTTTGA CGTCGGCCAG GCCGCGACCA CGACGCTGAA TGCGGCTAAT GAAGAGTCGG TTGCCGCGTT TCTGCATGGC GATATCCGCT TTACCGATAT TGCGGCGGTT AACCTGGCGG TGCTGGATAA GATGGACCTG CAGGAGCCGC AAAGCATTGA TGATGTTCTG GTTATCGACG CCGAGGCGCG CGCAATCGCT CACCAGCAGC TGCAGCGGTT GGTTGCGCAG GCGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT