SSGCID: Target Page

KlpnC.01095.a

Geranyltranstransferase

CENTER ID:	KlpnC.01095.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.01095.a.B1.GE44292	Full length(KlpnC.01095.a)		1	299

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.1080
UniProt:	A0A0H3GNH4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFPQQLQAC VEQANEALRR FIAPQPFQNT PLVEAMHYGA LLGGKRLRPF LVYATGEMFG VCRTTLDAPA AAVECIHAYS LMHDDLPAMD DDDLRRGLPT CHIKFGEANA ILAGDALQTL AFSILSDAPM VDVPDRDRLA MVSELAQASG VAGMCGGQAL DLQAEGQQVD LQALERIHRH KTGALIRAAV RMGALSAGER GRAALPALDR YAENIGLAFQ VQDDILDVVG DTATLGKRQG ADQQLGKSTY PALLGLEQAR KKAHDLIADA RRSLDELAAQ SLDTSALEAL ANYIIQRDK

NT Sequence

ATGGATTTCC CGCAACAATT GCAGGCCTGT GTGGAACAGG CAAACGAAGC GCTGCGCCGT TTCATCGCCC CGCAGCCCTT TCAGAACACT CCGCTGGTGG AAGCGATGCA TTATGGCGCA TTGTTAGGCG GTAAACGCCT GCGTCCCTTC CTTGTCTACG CCACCGGCGA GATGTTCGGC GTGTGCCGTA CCACGCTGGA TGCTCCTGCC GCGGCGGTCG AATGCATTCA TGCCTACTCC CTGATGCATG ATGATTTGCC GGCGATGGAT GATGACGATC TGCGCCGCGG CCTGCCGACC TGCCATATTA AATTTGGCGA AGCCAACGCA ATTCTTGCGG GAGATGCCCT GCAAACGCTG GCGTTCTCTA TACTGAGTGA TGCGCCGATG GTCGACGTTC CTGACCGGGA CCGGCTGGCG ATGGTTTCCG AACTGGCGCA GGCCAGCGGC GTGGCGGGCA TGTGCGGCGG CCAGGCGCTG GATCTGCAGG CCGAGGGACA GCAGGTTGAT TTACAGGCGC TGGAGCGTAT CCATCGCCAC AAAACGGGCG CCCTGATCCG CGCCGCCGTC CGGATGGGAG CCCTGAGCGC CGGCGAACGC GGCCGCGCTG CCCTGCCCGC GCTGGATCGC TATGCGGAAA ATATCGGTCT TGCCTTCCAG GTCCAGGATG ACATTCTCGA CGTGGTAGGG GATACTGCGA CCCTTGGCAA ACGTCAGGGT GCCGACCAGC AATTGGGTAA AAGCACCTAT CCCGCCCTCC TGGGTCTTGA GCAAGCCCGG AAGAAAGCGC ACGACCTGAT CGCTGATGCC CGCCGGTCGT TAGATGAGCT GGCCGCACAA TCTCTGGATA CTTCGGCACT GGAAGCGCTC GCGAATTACA TTATTCAGCG CGATAAA

Details for KlpnC.01095.a.B1.GE44292

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	92
PERCENT COVERAGE:	88

Validated AA Sequence

MAHHHHHHMD FPQQLQACVE QANEALRRFI APQPFQNTPL VEAMHYGALL GGKRLRPFLV YATGEMFGVC RTTLDAPAAA VECIHAYSLM HDDLPAMDDD DLRRGLPTCH IKFGEANAIL AGDALQTLAF SILSDAPMVD VPDRDRLAMV SELAQASGVA GMCGGQALDL QAEGQQVDLQ ALERIHRHKT GALIRAAVRM GALSAGERGR AALPALDRYA ENIGLAFQVQ DDILDVVXXT ATLGKRXGAD XXWVKXPXPP SWVLSKPGXK RTT

Validated NT Sequence

anannngact cnctataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt tcccgcaaca attgcaggcc tgtgtggaac aggcaaacga agcgctgcgc cgtttcatcg ccccgcagcc ctttcagaac actccgctgg tggaagcgat gcattatggc gcattgttag gcggtaaacg cctgcgtccc ttccttgtct acgccaccgg cgagatgttc ggcgtgtgcc gtaccacgct ggatgctcct gccgcggcgg tcgaatgcat tcatgcctac tccctgatgc atgatgattt gccggcgatg gatgatgacg atctgcgccg cggcctgccg acctgccata ttaaatttgg cgaagccaac gcaattcttg cgggagatgc cctgcaaacg ctggcgttct ctatactgag tgatgcgccg atggtcgacg ttcctgaccg ggaccggctg gcgatggttt ccgaactggc gcaggccagc ggcgtggcgg gcatgtgcgg cggccaggcg ctggatctgc aggccgaggg acagcaggtt gatttacagg cgctggagcg tatccatcgc cataaaacgg gcgccctgat ccgcgccgcc gtccggatgg gagccctgag cgccggcgaa cgcggccgcg ctgccctgcc cgcgctggat cgctatgcgg aaaatatcgg tcttgccttc caggtccagg atgacattct cgacgtggta nggnatactg cgacccttgg caaacgtcnn ggtgccgacc annattgggt aaaancacct atnccgccct cctgggtctt gagcaagccc ggaanaaagc gcacgacctg atcgctgatg cccgcngncn ttanatgagc tggccgcaca ntctctggga nacttcngca ctggaagcgc tcgcgaatta cattattnnn ncgcganant aanngagtan anaggatccn gctgctaacn ancncggaaa ggagctgant ngntgcngcc nccgctgagc a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt tcccgcaaca attgcaggcc tgtgtggaac aggcaaacga agcgctgcgc cgtttcatcg ccccgcagcc ctttcagaac actccgctgg tggaagcgat gcattatggc gcattgttag gcggtaaacg cctgcgtccc ttccttgtct acgccaccgg cgagatgttc ggcgtgtgcc gtaccacgct ggatgctcct gccgcggcgg tcgaatgcat tcatgcctac tccctgatgc atgatgattt gccggcgatg gatgatgacg atctgcgccg cggcctgccg acctgccata ttaaatttgg cgaagccaac gcaattcttg cgggagatgc cctgcaaacg ctggcgttct ctatactgag tgatgcgccg atggtcgacg ttcctgaccg ggaccggctg gcgatggttt ccgaactggc gcaggccagc ggcgtggcgg gcatgtgcgg cggccaggcg ctggatctgc aggccgaggg acagcaggtt gatttacagg cgctggagcg tatccatcgc cacaaaacgg gcgccctgat ccgcgccgcc gtccggatgg gagccctgag cgccggcgaa cgcggccgcg ctgccctgcc cgcgctggat cgctatgcgg aaaatatcgg tcttgccttc caggtccagg atgacattct cgacgtggta ggggatactg cgacccttgg caaacgtcag ggtgccgacc agcaattggg taaaagcacc tatcccgccc tcctgggtct tgagcaagcc cggaagaaag cgcacgacct gatcgctgat gcccgccggt cgttagatga gctggccgca caatctctgg atacttcggc actggaagcg ctcgcgaatt acattattca gcgcgataaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat