SSGCID: Target Page

KlpnC.01026.c

Glutamate-1-semialdehyde 2,1-aminomutase

CENTER ID:	KlpnC.01026.c
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.01026.c.B1.GE44291	Full length(KlpnC.01026.c)		1	426

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.875
UniProt:	A0A0H3GMT9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKSENLYHA ARELIPGGVN SPVRAFTGVG GTPLFIERAD GAYLYDVDGK AYIDYVGSWG PMVLGHNHPA IRNAVIEAAS RGLSFGAPTE MEVKMAALVT ELVPTMDMVR MVNSGTEATM SAIRLARGFT GRDKIIKFEG CYHGHADCLL VKAGSGALTL GQPNSPGVPA DFAKHTLTCT YNDLASVRAA FEQYPQEIAC IIVEPVAGNM NCIPPQPEFL PGLRALCDEF GALLIIDEVM TGFRVALAGA QAYYGVEPDL TCLGKIIGGG MPVGAFGGRR EVMDALAPTG PVYQAGTLSG NPIAMAAGFA CLNEVAQPGV HETLTELTNQ LAQGLLDAAR DAGIPLVVNN VGGMFGIFFT DAETVTCYQD VVKCDVERFK RFFHLMLEEG VYLAPSAFEA GFMSVAHSEE DIDNTIDAAR RVFAKL

NT Sequence

ATGAGCAAAT CTGAAAATCT GTATCACGCT GCGCGCGAAC TGATCCCCGG CGGCGTTAAC TCGCCGGTGC GCGCTTTCAC CGGCGTCGGC GGCACACCGC TGTTTATCGA ACGCGCGGAT GGCGCTTACC TCTATGACGT GGATGGGAAA GCCTATATCG ATTATGTCGG TTCCTGGGGT CCGATGGTGC TGGGCCACAA CCACCCGGCT ATCCGCAATG CGGTGATCGA GGCGGCATCC CGCGGCCTGA GCTTTGGCGC GCCGACCGAA ATGGAAGTGA AAATGGCGGC CCTGGTGACC GAGCTGGTGC CGACCATGGA TATGGTGCGG ATGGTGAACT CCGGTACCGA AGCCACCATG AGCGCCATTC GCCTGGCGCG CGGCTTCACC GGTCGCGACA AAATCATTAA GTTCGAAGGC TGCTACCACG GCCATGCCGA CTGCCTGCTG GTGAAAGCCG GTTCCGGCGC GTTGACCCTC GGCCAGCCGA ACTCGCCGGG CGTCCCGGCC GATTTCGCCA AACATACCCT GACCTGCACT TATAACGACC TCGCCTCGGT GCGCGCGGCG TTCGAGCAGT ATCCGCAGGA GATCGCCTGC ATCATCGTCG AGCCGGTGGC CGGAAATATG AACTGCATTC CGCCGCAGCC GGAATTCCTG CCGGGCCTGC GCGCCCTGTG CGACGAATTT GGCGCCCTGC TGATTATCGA CGAAGTGATG ACCGGCTTCC GCGTGGCGCT GGCAGGCGCT CAGGCTTACT ACGGCGTGGA GCCGGATCTG ACCTGTCTGG GGAAAATCAT CGGCGGCGGG ATGCCGGTAG GCGCCTTCGG CGGCCGCCGT GAGGTGATGG ACGCCCTGGC GCCCACCGGT CCGGTCTATC AGGCGGGCAC CCTATCCGGC AACCCGATCG CCATGGCGGC TGGCTTCGCC TGCCTGAACG AAGTGGCGCA GCCGGGGGTC CATGAAACCC TGACTGAACT GACTAACCAG CTGGCGCAAG GCCTGCTGGA TGCGGCGCGT GACGCCGGGA TCCCGCTGGT GGTCAATAAC GTCGGCGGCA TGTTCGGCAT CTTCTTTACC GATGCCGAAA CCGTCACCTG CTATCAGGAC GTGGTGAAGT GCGACGTTGA ACGATTCAAG CGCTTCTTCC ATCTGATGCT GGAGGAAGGC GTGTACCTGG CGCCGTCAGC GTTTGAGGCC GGCTTTATGT CCGTGGCCCA CAGCGAAGAA GATATCGACA ACACCATCGA CGCCGCGCGT CGGGTATTTG CCAAGCTG

Details for KlpnC.01026.c.B1.GE44291

HARVESTED ON:	5/21/2021
SEQUENCED ON:	6/4/2021
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	95
PERCENT COVERAGE:	61

Validated AA Sequence

Validated NT Sequence

gnnnnnngnn nnnnnnnnnn nnantatacg actcnctata gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatga gcaaatctga aaatctgtac cacgctgcgc gcgaactgat ccccggcggc gttaactcgc cggtgcgcgc tttcaccggc gtcggcggca caccgctgtt tatcgaacgc gcggatggcg cttacctcta tgacgtggat gggaaagcct atatcgatta tgtcggttcc tggggtccga tggtgctggg ccacaaccac ccggctatcc gcaatgcggt gatcgaggcg gcatcccgcg gcctgagctt tggcgcgccg accgaaatgg aagtgaaaat ggcggccctg gtgaccgagc tggtgccgac catggatatg gtgcggatgg tgaactccgg taccgaagcc accatgagcg ccattcgcct ggcgcgcggc ttcaccggtc gcgacaaaat cattaagttc gaaggctgct accncngcca tgccgactgc ctgctggtga aagccggttc cggcgcgttg accctcggcc agccgaactc gccgggcgtc ccggccgatt tcgccaaaca taccctgacc tgcacttata acgacctcgc ctcggtgcgc gcggcgttcg agcagtatcc gcangagatc gcctgcatca tcgtcgagcc ggtggccgga aatatgaact gcattccgcc gcagccggaa ttcctgccgg gcctgcgcgc gctgtgcgac gaatttggcg ccctgctgat tatcgacgaa gtgatgaccg gcttccgcgt ggcgctggca ngcgctcanc ttactacggc gtgganccgg atctgacctg tctggggaaa atcatcgtgg cgggatgccg gtaggcgcct tnnncggccg ccgngaggtg atggacgccc tgntcccnnc ggtccngtct atcnngnggg nnccctcnnn ggcaacccga tngcntgngc tggcttcncc tgcctgaacn aannggcncn nnngggnccn ngaaacccnn nntgaatgac naac

Expected Protein Sequence

MAHHHHHHMS KSENLYHAAR ELIPGGVNSP VRAFTGVGGT PLFIERADGA YLYDVDGKAY IDYVGSWGPM VLGHNHPAIR NAVIEAASRG LSFGAPTEME VKMAALVTEL VPTMDMVRMV NSGTEATMSA IRLARGFTGR DKIIKFEGCY HGHADCLLVK AGSGALTLGQ PNSPGVPADF AKHTLTCTYN DLASVRAAFE QYPQEIACII VEPVAGNMNC IPPQPEFLPG LRALCDEFGA LLIIDEVMTG FRVALAGAQA YYGVEPDLTC LGKIIGGGMP VGAFGGRREV MDALAPTGPV YQAGTLSGNP IAMAAGFACL NEVAQPGVHE TLTELTNQLA QGLLDAARDA GIPLVVNNVG GMFGIFFTDA ETVTCYQDVV KCDVERFKRF FHLMLEEGVY LAPSAFEAGF MSVAHSEEDI DNTIDAARRV FAKL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca aatctgaaaa tctgtatcac gctgcgcgcg aactgatccc cggcggcgtt aactcgccgg tgcgcgcttt caccggcgtc ggcggcacac cgctgtttat cgaacgcgcg gatggcgctt acctctatga cgtggatggg aaagcctata tcgattatgt cggttcctgg ggtccgatgg tgctgggcca caaccacccg gctatccgca atgcggtgat cgaggcggca tcccgcggcc tgagctttgg cgcgccgacc gaaatggaag tgaaaatggc ggccctggtg accgagctgg tgccgaccat ggatatggtg cggatggtga actccggtac cgaagccacc atgagcgcca ttcgcctggc gcgcggcttc accggtcgcg acaaaatcat taagttcgaa ggctgctacc acggccatgc cgactgcctg ctggtgaaag ccggttccgg cgcgttgacc ctcggccagc cgaactcgcc gggcgtcccg gccgatttcg ccaaacatac cctgacctgc acttataacg acctcgcctc ggtgcgcgcg gcgttcgagc agtatccgca ggagatcgcc tgcatcatcg tcgagccggt ggccggaaat atgaactgca ttccgccgca gccggaattc ctgccgggcc tgcgcgccct gtgcgacgaa tttggcgccc tgctgattat cgacgaagtg atgaccggct tccgcgtggc gctggcaggc gctcaggctt actacggcgt ggagccggat ctgacctgtc tggggaaaat catcggcggc gggatgccgg taggcgcctt cggcggccgc cgtgaggtga tggacgccct ggcgcccacc ggtccggtct atcaggcggg caccctatcc ggcaacccga tcgccatggc ggctggcttc gcctgcctga acgaagtggc gcagccgggg gtccatgaaa ccctgactga actgactaac cagctggcgc aaggcctgct ggatgcggcg cgtgacgccg ggatcccgct ggtggtcaat aacgtcggcg gcatgttcgg catcttcttt accgatgccg aaaccgtcac ctgctatcag gacgtggtga agtgcgacgt tgaacgattc aagcgcttct tccatctgat gctggaggaa ggcgtgtacc tggcgccgtc agcgtttgag gccggcttta tgtccgtggc ccacagcgaa gaagatatcg acaacaccat cgacgccgcg cgtcgggtat ttgccaagct gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat