KlpnC.01026.b
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
| CENTER ID: | KlpnC.01026.b |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.01026.b.B1.GE44290 | Full length(KlpnC.01026.b) | 1 | 429 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7UG3
DEPOSITED: 3/23/2022
DETERMINATION: XRay
CLONE: KlpnC.01026.b.B1.GE44290
PROTEIN: KlpnC.01026.b.B1.PW39017
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.1583 |
| UniProt: | A0A0H3GUB2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLDDLAFDR RHIWHPYTSM TSPLPVYPVV SAHGCELSLA GGEQLVDGMS SWWAAIHGYN HPRLNAALKG QIDQMSHVMF GGITHPPAVA LCRQLVAMTP ASLECVFLAD SGSVAVEVAM KMALQYWQAK GEPRRRFLTF RNGYHGDTFG AMSVCDPQNS MHSLWQGYLP DNLFAPAPQS RFDGEWDEMD MVPFARLMAA HRHEIAAVIL EPIVQGAGGM RMYHPEWLKR VRKMCDREGI LLIADEIATG FGRTGKLFAC EHAGITADIL CLGKALTGGT MTLSAAITTR TVAETISNGE AGCFMHGPTF MGNPLACAVA GESLRLLESG EWQPQVTAIE AQLQAELAPA RGSALVADVR VLGAIGVVET RRPVNMAALQ RFFVEQGVWI RPFGRLIYLM PPYIITPEQL TRLTRAVNQA VQDETFFSE
NT Sequence
ATGACACTGG ACGATCTCGC TTTCGATCGG CGCCACATCT GGCACCCCTA CACCTCTATG ACTTCCCCCC TGCCGGTCTA CCCGGTGGTG TCCGCCCACG GTTGCGAGCT GTCCCTCGCC GGCGGCGAAC AGTTGGTTGA CGGTATGTCT TCCTGGTGGG CGGCGATCCA CGGCTACAAT CACCCACGCC TCAATGCGGC GCTGAAGGGG CAAATTGACC AAATGTCGCA CGTGATGTTC GGCGGGATCA CCCATCCGCC GGCAGTAGCG CTATGCCGTC AGCTGGTGGC GATGACCCCG GCCTCGCTGG AGTGCGTTTT CCTCGCCGAC TCCGGTTCAG TGGCGGTAGA GGTGGCGATG AAAATGGCGC TGCAGTACTG GCAGGCTAAA GGCGAACCAC GTCGTCGTTT TCTGACCTTC CGCAACGGCT ATCACGGGGA TACCTTCGGA GCGATGTCGG TCTGCGATCC GCAGAACTCG ATGCACAGCC TGTGGCAGGG CTACCTGCCG GATAACCTGT TCGCCCCCGC GCCGCAAAGC CGCTTTGACG GCGAGTGGGA CGAGATGGAT ATGGTGCCGT TTGCCCGCCT GATGGCCGCG CATCGTCATG AAATCGCCGC GGTGATCCTC GAACCTATCG TCCAGGGGGC GGGCGGCATG CGGATGTACC ATCCGGAGTG GCTGAAGCGG GTGCGTAAAA TGTGCGACCG GGAAGGGATA CTGCTGATCG CCGATGAGAT TGCCACCGGC TTTGGACGCA CCGGCAAGCT GTTCGCTTGT GAACACGCCG GGATTACCGC CGACATTCTG TGTCTGGGCA AAGCGCTGAC CGGCGGCACC ATGACCCTTT CCGCCGCCAT CACCACCCGA ACGGTTGCTG AAACCATTAG CAATGGCGAA GCCGGTTGCT TTATGCACGG CCCCACCTTT ATGGGCAATC CGCTGGCCTG CGCGGTGGCC GGCGAAAGCC TGCGCCTGCT GGAGAGCGGC GAATGGCAGC CGCAGGTGAC GGCGATTGAA GCCCAGCTGC AGGCCGAGCT GGCCCCGGCG CGGGGATCTG CGCTGGTCGC GGACGTCCGC GTGCTGGGCG CTATTGGCGT GGTTGAAACC CGTCGCCCGG TCAACATGGC CGCCCTGCAG CGCTTTTTCG TCGAGCAGGG AGTCTGGATC CGTCCGTTTG GCCGGCTGAT TTATCTGATG CCGCCGTACA TCATCACGCC CGAGCAGCTG ACCCGCCTGA CCCGTGCGGT CAACCAGGCA GTGCAGGACG AAACATTTTT TAGCGAA
Details for KlpnC.01026.b.B1.GE44290
| HARVESTED ON: | 5/21/2021 |
| SEQUENCED ON: | 6/4/2021 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 71 |
Validated AA Sequence
MAHHHHHHHM TLDDLAFDRR HIWHPYTSMT SPLPVYPVVS AHGCELSLAG GEQLVDGMSS WWAAIHGYNH PRLNAALKGQ IDQMSHVMFG GITHPPAVAL CRQLVAMTPA SLECVFLADS GSVAVEVAMK MALQYWQAKG EPRRRFLTFR NGYHGDTFGA MSVCDPQNSM HSLWQGYLPD NLFAPAPQSR FDGEWDEIDM VPFARLMAAH RHEIAAVILE PIVQGAGGMR MYHPEWLKRV RKMCDREGIL LIADEIATGF GRTGKLFACE HAGITADILC LGKALTGGTM TLSAAITTRT VAETISNGEA GCF
Validated NT Sequence
atggctcacc accaccacca ccaccatatg acactggacg atctcgcttt cgatcggcgc cacatctggc acccctacac ctctatgact tcccccctgc cggtctaccc ggtggtgtcc gcccacggtt gcgagctgtc cctcgccggc ggcgaacagt tggttgatgg tatgtcttcc tggtgggcgg cgatccacgg ctacaatcac ccacgcctca atgcggcgct gaagggacaa attgaccaaa tgtcgcacgt gatgttcggc gggatcaccc atccgccggc agtagcgcta tgccgtcagc tggtggcgat gaccccggcc tcgctggagt gcgttttcct cgccgactcc ggttcagtgg cggtagaggt ggcgatgaaa atggcgctgc agtactggca agctaaaggc gaaccacgtc gtcgttttct gaccttccgc aacggctatc acggggatac cttcggagcg atgtcggtct gcgatccgca gaactcgatg cacagcctgt ggcagggcta cctgccggat aatcttttcg cccccgcgcc gcaaagccgc ttcgacggcg agtgggacga gatagatatg gtgycgtttg cccgcctgat ggccgcgcat cgtcatgaaa tcgccgcagt gatcctcgaa cctatcgtcc arggggcggg cggcatgcgg atgtaccatc cggagtggct gaarcgggtg cgtaaaawgt gcgaccggga agggatactg ctgatcgccg atgagattgc caccggcttt ggncgcaccg gcaagmtgtt cgcttgtgaa cacgccggga ttaccgccga cattctgtgt ctgggcaaag cgctgaccgg cggcwccatg accctttccg ccgccatcac tacccgaacg gttgctgaaa ccattarmaa tggsgaagcc ggttgcttta tggctcacca ccaccaccac caccatatga cactggacga tctcgctttc gatcggcgcc acatctggca cccctacacc tctatgactt cccccctgcc ggtctacccg gtggtgtccg cccacggttg cgagctgtcc ctcgccggcg gcgaacagtt ggttgatggt atgtcttcct ggtgggcggc gatccacggc tacaatcacc cacgcctcaa tgcggcgctg aagggacaaa ttgaccaaat gtcgcacgtg atgttcggcg ggatcaccca tccgccggca gtagcgctat gccgtcagct ggtggcgatg accccggcct cgctggagtg cgttttcctc gccgactccg gttcagtggc ggtagaggtg gcgatgaaaa tggcgctgca gtactggcaa gctaaaggcg aaccacgtcg tcgttttctg accttccgca acggctatca cggggatacc ttcggagcga tgtcggtctg cgatccgcag aactcgatgc acagcctgtg gcagggctac ctgccggata atcttttcgc ccccgcgccg caaagccgct tcgacggcga gtgggacgag atagatatgg tgccgtttgc ccgcctgatg gccgcgcatc gtcatgaaat cgccgcagtg atcctcgaac ctatcgtcca gggggcgggc ggcatgcgga tgtaccatcc ggagtggctg aagcgggtgc gtaaaatgtg cgaccgggaa gggatactgc tgatcgccga tgagattgcc accggctttg gncgcaccgg caagctgttc gcttgtgaac acgccgggat taccgccgac attctgtgtc tgggcaaagc gctgaccggc ggcaccatga ccctttccgc cgccatcact acccgaacgg ttgctgaaac cattagcaat ggcgaagccg gttgcttt
Expected Protein Sequence
MAHHHHHHMT LDDLAFDRRH IWHPYTSMTS PLPVYPVVSA HGCELSLAGG EQLVDGMSSW WAAIHGYNHP RLNAALKGQI DQMSHVMFGG ITHPPAVALC RQLVAMTPAS LECVFLADSG SVAVEVAMKM ALQYWQAKGE PRRRFLTFRN GYHGDTFGAM SVCDPQNSMH SLWQGYLPDN LFAPAPQSRF DGEWDEMDMV PFARLMAAHR HEIAAVILEP IVQGAGGMRM YHPEWLKRVR KMCDREGILL IADEIATGFG RTGKLFACEH AGITADILCL GKALTGGTMT LSAAITTRTV AETISNGEAG CFMHGPTFMG NPLACAVAGE SLRLLESGEW QPQVTAIEAQ LQAELAPARG SALVADVRVL GAIGVVETRR PVNMAALQRF FVEQGVWIRP FGRLIYLMPP YIITPEQLTR LTRAVNQAVQ DETFFSE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac tggacgatct cgctttcgat cggcgccaca tctggcaccc ctacacctct atgacttccc ccctgccggt ctacccggtg gtgtccgccc acggttgcga gctgtccctc gccggcggcg aacagttggt tgacggtatg tcttcctggt gggcggcgat ccacggctac aatcacccac gcctcaatgc ggcgctgaag gggcaaattg accaaatgtc gcacgtgatg ttcggcggga tcacccatcc gccggcagta gcgctatgcc gtcagctggt ggcgatgacc ccggcctcgc tggagtgcgt tttcctcgcc gactccggtt cagtggcggt agaggtggcg atgaaaatgg cgctgcagta ctggcaggct aaaggcgaac cacgtcgtcg ttttctgacc ttccgcaacg gctatcacgg ggataccttc ggagcgatgt cggtctgcga tccgcagaac tcgatgcaca gcctgtggca gggctacctg ccggataacc tgttcgcccc cgcgccgcaa agccgctttg acggcgagtg ggacgagatg gatatggtgc cgtttgcccg cctgatggcc gcgcatcgtc atgaaatcgc cgcggtgatc ctcgaaccta tcgtccaggg ggcgggcggc atgcggatgt accatccgga gtggctgaag cgggtgcgta aaatgtgcga ccgggaaggg atactgctga tcgccgatga gattgccacc ggctttggac gcaccggcaa gctgttcgct tgtgaacacg ccgggattac cgccgacatt ctgtgtctgg gcaaagcgct gaccggcggc accatgaccc tttccgccgc catcaccacc cgaacggttg ctgaaaccat tagcaatggc gaagccggtt gctttatgca cggccccacc tttatgggca atccgctggc ctgcgcggtg gccggcgaaa gcctgcgcct gctggagagc ggcgaatggc agccgcaggt gacggcgatt gaagcccagc tgcaggccga gctggccccg gcgcggggat ctgcgctggt cgcggacgtc cgcgtgctgg gcgctattgg cgtggttgaa acccgtcgcc cggtcaacat ggccgccctg cagcgctttt tcgtcgagca gggagtctgg atccgtccgt ttggccggct gatttatctg atgccgccgt acatcatcac gcccgagcag ctgacccgcc tgacccgtgc ggtcaaccag gcagtgcagg acgaaacatt ttttagcgaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat