KlpnC.01026.a

4-aminobutyrate aminotransferase

CENTER ID: KlpnC.01026.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.01026.a.B1.GE44289 Full length(KlpnC.01026.a) 1 427
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.953
UniProt: A0A0H3GSB6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSNKAMMAR RSDAVPRGVG QIHPIFAERA ENCRVWDVEG REYLDFAGGI AVLNTGHLHP QVVAAVEDQL KKLSHTCFQV LAYEPYLALC EKMNQKVPGD FAKKTLLVTT GSEAVENAVK IARAATGRSG AIAFTGAYHG RTHYTLSLTG KVNPYSAGMG LMPGHVYRAL YPCALHGVSD DEAIASIHRI FKNDAAPEDI AAIIIEPVQG EGGFYAASPA FMQRLRALCD EHGIMLIADE VQSGAGRTGT LFAMEQMGVA ADITTFAKSI AGGFPLAGVT GRAEVMDAIA PGGLGGTYAG NPIACAAALA VLQIFEQENL LEKANQLGDT LRQGLLAIAE DHPEIGDVRG LGAMIAIELF EEGDRSRPNA RLTADIVARA RDKGLILLSC GPYYNVLRIL VPLTIEEAQI EQGLKIIADC FSEAKQA
NT Sequence
ATGAACAGCA ATAAAGCAAT GATGGCGCGT CGCAGCGACG CGGTCCCGCG CGGCGTCGGC CAGATCCACC CTATCTTCGC CGAACGCGCG GAGAACTGCC GGGTGTGGGA CGTGGAAGGC CGGGAATATC TCGATTTTGC CGGCGGTATC GCGGTGCTCA ATACCGGCCA CCTGCATCCG CAGGTTGTCG CCGCCGTCGA AGACCAGCTG AAAAAGCTGT CGCATACCTG CTTCCAGGTG CTGGCCTATG AGCCTTATCT GGCGCTGTGC GAAAAGATGA ATCAAAAGGT GCCCGGTGAT TTCGCCAAGA AAACCCTGCT GGTCACCACC GGGTCGGAAG CGGTGGAGAA CGCGGTGAAG ATCGCCCGCG CGGCAACCGG GCGCAGCGGC GCTATCGCCT TCACCGGCGC CTATCACGGC CGCACCCATT ACACCCTGTC GCTGACCGGC AAAGTGAATC CTTACTCCGC CGGTATGGGC TTAATGCCGG GCCATGTCTA TCGCGCCCTC TACCCATGCG CGCTGCACGG CGTCAGTGAT GACGAGGCGA TCGCCAGCAT CCATCGCATC TTCAAAAACG ATGCCGCGCC GGAAGATATC GCCGCCATCA TTATTGAGCC GGTGCAGGGT GAAGGCGGGT TCTATGCCGC CTCCCCGGCC TTTATGCAGC GCCTGCGCGC CCTGTGCGAC GAGCACGGCA TTATGCTCAT CGCCGATGAG GTCCAGAGCG GCGCGGGCCG CACCGGTACC CTGTTCGCCA TGGAGCAGAT GGGCGTCGCG GCGGATATCA CCACCTTCGC CAAATCCATC GCCGGCGGTT TCCCGCTGGC GGGCGTCACC GGCCGCGCGG AGGTGATGGA CGCCATCGCC CCCGGCGGAC TGGGCGGCAC CTACGCCGGC AACCCCATCG CCTGCGCCGC CGCCCTGGCG GTATTACAGA TTTTCGAGCA GGAGAATCTG CTGGAAAAAG CCAACCAGCT CGGCGACACC CTGCGCCAGG GCCTGCTGGC GATCGCTGAA GATCATCCGG AGATCGGCGA CGTGCGCGGT CTGGGGGCGA TGATCGCCAT TGAGCTGTTT GAAGAGGGCG ACCGCAGCAG GCCGAACGCC AGGCTGACGG CGGATATCGT CGCCCGCGCC CGCGACAAAG GCCTGATCCT GCTCTCCTGC GGGCCGTACT ACAACGTGCT GCGCATCCTG GTGCCGCTGA CCATCGAAGA GGCGCAGATC GAGCAGGGGC TCAAGATCAT CGCCGACTGC TTTAGCGAGG CGAAACAGGC A
Details for KlpnC.01026.a.B1.GE44289
HARVESTED ON: 5/21/2021
SEQUENCED ON: 6/4/2021
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 78
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMN SNKAMMARRS DAVPRGVGQI HPIFAERAEN CRVWDVEGRE YLDFAGGIAV LNTGHLHPQV VAAVEDQLKK LSHTCFQVLA YEPYLALCEK MNQKVPGDFA KKTLLVTTGS EAVENAVKIA RAATGRSGAI AFTGAYHGRT HYTLSLTGKV NPYSAGMGLM PGHVYRALYP CALHGVSDDE AIASXHRIFX NDAAPEDIAA XIIEPVXGXX GFYAASPAXM QRLRALCXEH GIMLIADEVQ XGAGRTXYPX XHGXDGXRGG XSPPXXXXXX GGFRWXXGHR PXRXXWXXXA PXGXGXXLXX XPHRXAAALX XXXNFXXXXI CXKSX
Validated NT Sequence
atacgactcn ctatagggag accacaacgg tttccctcta gaaataattt tgtttaactt tangaaggag atataccatg gctcaccacc accaccacca tatgaacagc aataaagcaa tgatggcgcg tcgcagcgac gcggtaccgc gcggcgtcgg ccagatccac cctatcttcg ccgaacgcgc ggagaactgc cgggtgtggg acgtggaagg ccgggaatat ctcgattttg ccggcggtat cgcggtgctc aataccggcc acctgcatcc gcaggttgtc gccgccgtcg aagaccagct gaaaaagctg tcgcatacct gcttccaggt gctggcctat gagccttatc tggcgctgtg cgaaaagatg aatcaaaagg tgcccggtga tttcgccaag aaaaccctgc tggtcaccac cgggtcggaa gcggtggaga acgcggtgaa gatcgcccgc gcggcaaccg ggcgcagcgg cgctatcgcc ttcaccggcg cctatcacgg ccgcacccat tacaccctgt cgctgaccgg caaagtgaat ccttactccg ccggtatggg cttaatgccg ggccatgtct atcgcgccct ctacccgtgc gcgctgcacg gcgtcagtga tgacgaggcg atcgccagcn tccatcgcat cttcnaaaac gatgccgcgc cggaagatat cgccgccntc attattgagc cagtgcangg tgnaagnggg ttctatgccg cctccccngc cnttatgcag cgcctgcgcg ccctgtgcna cgagcacggc attatgctta tcgccgatga ggtccaganc ggcgcgggcc gcaccnggta ccctgntngc catggancag atgggngtcg cggcggannn tcnccncctt nnnccnaatn nntcnccggc gggttccgct ggngggncgg tcaccggccn ncgcgnangt natggaannn nntcgccccc gnngganntg ggnggcanct acnnnggnaa ccccatcgcn gcgccgccgc cctggnggnn tnnnngaatt ttcnnncagn anaaatctgc tgnaaaagcc nnanc
Expected Protein Sequence
MAHHHHHHMN SNKAMMARRS DAVPRGVGQI HPIFAERAEN CRVWDVEGRE YLDFAGGIAV LNTGHLHPQV VAAVEDQLKK LSHTCFQVLA YEPYLALCEK MNQKVPGDFA KKTLLVTTGS EAVENAVKIA RAATGRSGAI AFTGAYHGRT HYTLSLTGKV NPYSAGMGLM PGHVYRALYP CALHGVSDDE AIASIHRIFK NDAAPEDIAA IIIEPVQGEG GFYAASPAFM QRLRALCDEH GIMLIADEVQ SGAGRTGTLF AMEQMGVAAD ITTFAKSIAG GFPLAGVTGR AEVMDAIAPG GLGGTYAGNP IACAAALAVL QIFEQENLLE KANQLGDTLR QGLLAIAEDH PEIGDVRGLG AMIAIELFEE GDRSRPNARL TADIVARARD KGLILLSCGP YYNVLRILVP LTIEEAQIEQ GLKIIADCFS EAKQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca gcaataaagc aatgatggcg cgtcgcagcg acgcggtccc gcgcggcgtc ggccagatcc accctatctt cgccgaacgc gcggagaact gccgggtgtg ggacgtggaa ggccgggaat atctcgattt tgccggcggt atcgcggtgc tcaataccgg ccacctgcat ccgcaggttg tcgccgccgt cgaagaccag ctgaaaaagc tgtcgcatac ctgcttccag gtgctggcct atgagcctta tctggcgctg tgcgaaaaga tgaatcaaaa ggtgcccggt gatttcgcca agaaaaccct gctggtcacc accgggtcgg aagcggtgga gaacgcggtg aagatcgccc gcgcggcaac cgggcgcagc ggcgctatcg ccttcaccgg cgcctatcac ggccgcaccc attacaccct gtcgctgacc ggcaaagtga atccttactc cgccggtatg ggcttaatgc cgggccatgt ctatcgcgcc ctctacccat gcgcgctgca cggcgtcagt gatgacgagg cgatcgccag catccatcgc atcttcaaaa acgatgccgc gccggaagat atcgccgcca tcattattga gccggtgcag ggtgaaggcg ggttctatgc cgcctccccg gcctttatgc agcgcctgcg cgccctgtgc gacgagcacg gcattatgct catcgccgat gaggtccaga gcggcgcggg ccgcaccggt accctgttcg ccatggagca gatgggcgtc gcggcggata tcaccacctt cgccaaatcc atcgccggcg gtttcccgct ggcgggcgtc accggccgcg cggaggtgat ggacgccatc gcccccggcg gactgggcgg cacctacgcc ggcaacccca tcgcctgcgc cgccgccctg gcggtattac agattttcga gcaggagaat ctgctggaaa aagccaacca gctcggcgac accctgcgcc agggcctgct ggcgatcgct gaagatcatc cggagatcgg cgacgtgcgc ggtctggggg cgatgatcgc cattgagctg tttgaagagg gcgaccgcag caggccgaac gccaggctga cggcggatat cgtcgcccgc gcccgcgaca aaggcctgat cctgctctcc tgcgggccgt actacaacgt gctgcgcatc ctggtgccgc tgaccatcga agaggcgcag atcgagcagg ggctcaagat catcgccgac tgctttagcg aggcgaaaca ggcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat