KlpnC.00906.a
Cystathionine beta-lyase
| CENTER ID: | KlpnC.00906.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.00906.a.B1.GE44247 | Full length(KlpnC.00906.a) | 1 | 395 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.00906.a.B1.PW38994 | Full length(KlpnC.00906.a) | 1 | 395 |
Structures
8DUY
DEPOSITED: 7/27/2022
DETERMINATION: XRay
CLONE: KlpnC.00906.a.B1.GE44247
PROTEIN: KlpnC.00906.a.B1.PW38994
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.4447 |
| UniProt: | A0A0H3GVN6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADKHLDTAL VNAGRSKKYT QGSVNSVIQR ASSLVFDTVE AKKHATRNRA NGELFYGRRG TLTHFSLQEA MCELEGGAGC ALFPCGAAAV ANTILAFVEQ GDHVLMTNTA YEPSQDFCTK ILAKLGVTTS WFDPLIGADI ARLVRPETRV VFLESPGSIT MEVHDVPAIV AAVRQVAPEA IIMIDNTWAA GILFKALDFG IDISIQAGTK YLIGHSDAMV GTAVANARCW PQLRENAYLM GQMLDADTAY MTSRGLRTLG VRLRQHHESS LRIAEWLAQH PQVARVNHPA LPGSKGHEFW KRDFTGSSGL FSFVLSKRLN DAELAEYLDN FSLFSMAYSW GGFESLILAN QPEQIAHIRP DAEVDFSGTL IRLHIGLENV DDLQADLAAG FARIV
NT Sequence
ATGGCTGATA AACATCTTGA TACCGCGCTG GTCAACGCCG GGCGGAGCAA AAAGTACACC CAGGGCTCAG TGAACAGCGT TATCCAGCGC GCGTCATCGC TGGTTTTCGA CACCGTCGAG GCCAAAAAGC ACGCCACCCG CAATCGGGCC AACGGCGAGC TGTTCTACGG TCGTCGCGGC ACCCTGACCC ATTTCTCGCT GCAGGAAGCG ATGTGCGAGC TGGAAGGCGG CGCCGGCTGC GCCCTCTTCC CCTGCGGCGC CGCGGCGGTG GCTAACACTA TCCTGGCGTT TGTCGAGCAA GGCGACCATG TGCTGATGAC CAATACCGCC TATGAGCCAA GCCAGGACTT TTGTACCAAA ATTCTCGCCA AACTCGGCGT CACCACCAGC TGGTTCGATC CCTTAATCGG CGCCGATATC GCCCGTCTGG TTCGCCCTGA GACCCGCGTG GTGTTCCTCG AATCGCCCGG CTCGATCACC ATGGAAGTGC ACGATGTGCC GGCGATAGTC GCCGCCGTGC GTCAGGTCGC CCCGGAAGCG ATTATCATGA TCGATAACAC CTGGGCGGCG GGGATCCTGT TTAAAGCCCT GGATTTTGGC ATTGATATTT CCATTCAGGC AGGCACCAAA TACCTGATCG GCCATTCCGA CGCCATGGTG GGCACCGCGG TGGCGAACGC GCGCTGCTGG CCGCAGCTGC GTGAAAATGC CTACCTGATG GGGCAAATGC TGGACGCCGA TACTGCCTAT ATGACCAGCC GCGGCCTGCG CACCCTGGGC GTGCGCCTGC GTCAGCATCA TGAAAGCAGC CTGCGCATCG CCGAGTGGCT GGCGCAGCAC CCGCAGGTGG CGCGGGTAAA CCACCCCGCC CTGCCCGGCA GTAAAGGTCA TGAGTTCTGG AAACGTGATT TCACCGGCAG CAGCGGGCTA TTCTCGTTTG TGCTCAGCAA GCGCCTCAAT GATGCCGAAC TGGCGGAGTA CCTCGACAAT TTCAGCCTGT TCAGTATGGC CTATTCGTGG GGCGGCTTTG AGTCGCTGAT TCTCGCCAAC CAGCCGGAGC AAATCGCCCA CATTCGACCG GATGCTGAAG TCGACTTCAG CGGCACGCTG ATCCGCCTGC ACATTGGTCT CGAAAATGTT GACGATCTGC AGGCGGATTT AGCAGCGGGC TTCGCGCGTA TCGTG
Details for KlpnC.00906.a.B1.GE44247
| HARVESTED ON: | 4/1/2021 |
| SEQUENCED ON: | 4/13/2021 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMA DKHLDTALVN AGRSKKYTQG SVNSVIQRAS SLVFDTVEAK KHATRNRANG ELFYGRRGTL THFSLQEAMC ELEGGAGCAL FPCGAAAVAN TILAFVEQGD HVLMTNTAYE PSQDFCTKIL AKLGVTTSWF DPLIGADIAR LVRPETRVVF LESPGSITME VHDVPAIVAA VRQVAPEAII MIDNTWAAGI LFKALDFGID ISIQAGTKYL IGHSDAMVGT AVANARCWPQ LRENAYLMGQ MLDADTAYMT SRGLRTLGVR LRQHHESSLR IAEWLAQHPQ VARVNHPALP GSKGHEFWKR DFTGSSGLFS FVLSKRLNDA ELAEYLDNFS LFSMAYSWGG FESLILANQP EQIAHIRPDA EVDFSGTLIR LHIGLENVDD LQADL
Validated NT Sequence
tgntaaatcc gcctgcagat cgtcaacatt ttcgagacca atgtgcaggc ggatcagcgt gccgctgaag tcgacttcag catccggtcg aatgtgggcg atttgctccg gctggttggc gagaatcagc gactcaaagc cgccccacga ataggccata ctgaacaggc tgaaattgtc gaggtactcc gccagttcgg catcattgag gcgcttgctg agcacaaacg agaatagccc gctgctgccg gtgaaatcac gtttccagaa ctcatgacct ttactgccgg gcagggcggg gtggtttacc cgcgccacct gcgggtgctg cgccagccac tcggcgatgc gcaggctgct ttcatgatgc tgacgcaggc gcacgcccag ggtgcgcagg ccgcggctgg tcatataggc agtatcggcg tccagcattt gccccatcag gtaggcattt tcacgcagct gcggccagca gcgcgcgttc gccaccgcgg tgcccaccat ggcgtcggaa tggccgatca ggtatttggt gcctgcctga atggaaatat caatgccaaa atccagggct ttaaacagga tccccgccgc ccaggtgtta tcgatcatga taatcgcttc cggggcgacc tgacgcacgg cggcgactat cgccggcaca tcgtgcactt ccatggtgat cgagccgggc gattcgagga acaccacgcg ggtctcaggg cgaaccagac gggcgatatc ggcgccgatt aagggatcga accagctggt ggtgacgccg agtttggcga gaattttggt acaaaagtcc tggcttggct cataggcggt attggtcatc agcacatggt cgccttgctc gacaaacgcc aggatagtgt tagccaccgc cgcggcgccg caggggaaga gggcgcagcc ggcgccgcct tccagctcgc acatcgcttc ctgcagcgag aaatgggtca gggtgccgcg acgaccgtag aacagctcgc cgttggcccg attgcgggtg gcgtgctttt tggcctcgac ggtgtcgaaa accagcgatg acgcgcgctg gataacgctg ttcactgagc cctgggtgta ctttttgctc cgcccggcgt tgaccagcgc ggtatcaaga tgtttatcag ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMA DKHLDTALVN AGRSKKYTQG SVNSVIQRAS SLVFDTVEAK KHATRNRANG ELFYGRRGTL THFSLQEAMC ELEGGAGCAL FPCGAAAVAN TILAFVEQGD HVLMTNTAYE PSQDFCTKIL AKLGVTTSWF DPLIGADIAR LVRPETRVVF LESPGSITME VHDVPAIVAA VRQVAPEAII MIDNTWAAGI LFKALDFGID ISIQAGTKYL IGHSDAMVGT AVANARCWPQ LRENAYLMGQ MLDADTAYMT SRGLRTLGVR LRQHHESSLR IAEWLAQHPQ VARVNHPALP GSKGHEFWKR DFTGSSGLFS FVLSKRLNDA ELAEYLDNFS LFSMAYSWGG FESLILANQP EQIAHIRPDA EVDFSGTLIR LHIGLENVDD LQADLAAGFA RIV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg ataaacatct tgataccgcg ctggtcaacg ccgggcggag caaaaagtac acccagggct cagtgaacag cgttatccag cgcgcgtcat cgctggtttt cgacaccgtc gaggccaaaa agcacgccac ccgcaatcgg gccaacggcg agctgttcta cggtcgtcgc ggcaccctga cccatttctc gctgcaggaa gcgatgtgcg agctggaagg cggcgccggc tgcgccctct tcccctgcgg cgccgcggcg gtggctaaca ctatcctggc gtttgtcgag caaggcgacc atgtgctgat gaccaatacc gcctatgagc caagccagga cttttgtacc aaaattctcg ccaaactcgg cgtcaccacc agctggttcg atcccttaat cggcgccgat atcgcccgtc tggttcgccc tgagacccgc gtggtgttcc tcgaatcgcc cggctcgatc accatggaag tgcacgatgt gccggcgata gtcgccgccg tgcgtcaggt cgccccggaa gcgattatca tgatcgataa cacctgggcg gcggggatcc tgtttaaagc cctggatttt ggcattgata tttccattca ggcaggcacc aaatacctga tcggccattc cgacgccatg gtgggcaccg cggtggcgaa cgcgcgctgc tggccgcagc tgcgtgaaaa tgcctacctg atggggcaaa tgctggacgc cgatactgcc tatatgacca gccgcggcct gcgcaccctg ggcgtgcgcc tgcgtcagca tcatgaaagc agcctgcgca tcgccgagtg gctggcgcag cacccgcagg tggcgcgggt aaaccacccc gccctgcccg gcagtaaagg tcatgagttc tggaaacgtg atttcaccgg cagcagcggg ctattctcgt ttgtgctcag caagcgcctc aatgatgccg aactggcgga gtacctcgac aatttcagcc tgttcagtat ggcctattcg tggggcggct ttgagtcgct gattctcgcc aaccagccgg agcaaatcgc ccacattcga ccggatgctg aagtcgactt cagcggcacg ctgatccgcc tgcacattgg tctcgaaaat gttgacgatc tgcaggcgga tttagcagcg ggcttcgcgc gtatcgtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for KlpnC.00906.a.B1.PW38994
| PURIFICATION DATe: | 5/10/2021 |
| CONCENTRATION: | 27mg/ml |
| OBSERVED MW: | 44kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DKHLDTALVN AGRSKKYTQG SVNSVIQRAS SLVFDTVEAK KHATRNRANG ELFYGRRGTL THFSLQEAMC ELEGGAGCAL FPCGAAAVAN TILAFVEQGD HVLMTNTAYE PSQDFCTKIL AKLGVTTSWF DPLIGADIAR LVRPETRVVF LESPGSITME VHDVPAIVAA VRQVAPEAII MIDNTWAAGI LFKALDFGID ISIQAGTKYL IGHSDAMVGT AVANARCWPQ LRENAYLMGQ MLDADTAYMT SRGLRTLGVR LRQHHESSLR IAEWLAQHPQ VARVNHPALP GSKGHEFWKR DFTGSSGLFS FVLSKRLNDA ELAEYLDNFS LFSMAYSWGG FESLILANQP EQIAHIRPDA EVDFSGTLIR LHIGLENVDD LQADL
Validated NT Sequence
tgntaaatcc gcctgcagat cgtcaacatt ttcgagacca atgtgcaggc ggatcagcgt gccgctgaag tcgacttcag catccggtcg aatgtgggcg atttgctccg gctggttggc gagaatcagc gactcaaagc cgccccacga ataggccata ctgaacaggc tgaaattgtc gaggtactcc gccagttcgg catcattgag gcgcttgctg agcacaaacg agaatagccc gctgctgccg gtgaaatcac gtttccagaa ctcatgacct ttactgccgg gcagggcggg gtggtttacc cgcgccacct gcgggtgctg cgccagccac tcggcgatgc gcaggctgct ttcatgatgc tgacgcaggc gcacgcccag ggtgcgcagg ccgcggctgg tcatataggc agtatcggcg tccagcattt gccccatcag gtaggcattt tcacgcagct gcggccagca gcgcgcgttc gccaccgcgg tgcccaccat ggcgtcggaa tggccgatca ggtatttggt gcctgcctga atggaaatat caatgccaaa atccagggct ttaaacagga tccccgccgc ccaggtgtta tcgatcatga taatcgcttc cggggcgacc tgacgcacgg cggcgactat cgccggcaca tcgtgcactt ccatggtgat cgagccgggc gattcgagga acaccacgcg ggtctcaggg cgaaccagac gggcgatatc ggcgccgatt aagggatcga accagctggt ggtgacgccg agtttggcga gaattttggt acaaaagtcc tggcttggct cataggcggt attggtcatc agcacatggt cgccttgctc gacaaacgcc aggatagtgt tagccaccgc cgcggcgccg caggggaaga gggcgcagcc ggcgccgcct tccagctcgc acatcgcttc ctgcagcgag aaatgggtca gggtgccgcg acgaccgtag aacagctcgc cgttggcccg attgcgggtg gcgtgctttt tggcctcgac ggtgtcgaaa accagcgatg acgcgcgctg gataacgctg ttcactgagc cctgggtgta ctttttgctc cgcccggcgt tgaccagcgc ggtatcaaga tgtttatcag ccatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMA DKHLDTALVN AGRSKKYTQG SVNSVIQRAS SLVFDTVEAK KHATRNRANG ELFYGRRGTL THFSLQEAMC ELEGGAGCAL FPCGAAAVAN TILAFVEQGD HVLMTNTAYE PSQDFCTKIL AKLGVTTSWF DPLIGADIAR LVRPETRVVF LESPGSITME VHDVPAIVAA VRQVAPEAII MIDNTWAAGI LFKALDFGID ISIQAGTKYL IGHSDAMVGT AVANARCWPQ LRENAYLMGQ MLDADTAYMT SRGLRTLGVR LRQHHESSLR IAEWLAQHPQ VARVNHPALP GSKGHEFWKR DFTGSSGLFS FVLSKRLNDA ELAEYLDNFS LFSMAYSWGG FESLILANQP EQIAHIRPDA EVDFSGTLIR LHIGLENVDD LQADLAAGFA RIV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG ATAAACATCT TGATACCGCG CTGGTCAACG CCGGGCGGAG CAAAAAGTAC ACCCAGGGCT CAGTGAACAG CGTTATCCAG CGCGCGTCAT CGCTGGTTTT CGACACCGTC GAGGCCAAAA AGCACGCCAC CCGCAATCGG GCCAACGGCG AGCTGTTCTA CGGTCGTCGC GGCACCCTGA CCCATTTCTC GCTGCAGGAA GCGATGTGCG AGCTGGAAGG CGGCGCCGGC TGCGCCCTCT TCCCCTGCGG CGCCGCGGCG GTGGCTAACA CTATCCTGGC GTTTGTCGAG CAAGGCGACC ATGTGCTGAT GACCAATACC GCCTATGAGC CAAGCCAGGA CTTTTGTACC AAAATTCTCG CCAAACTCGG CGTCACCACC AGCTGGTTCG ATCCCTTAAT CGGCGCCGAT ATCGCCCGTC TGGTTCGCCC TGAGACCCGC GTGGTGTTCC TCGAATCGCC CGGCTCGATC ACCATGGAAG TGCACGATGT GCCGGCGATA GTCGCCGCCG TGCGTCAGGT CGCCCCGGAA GCGATTATCA TGATCGATAA CACCTGGGCG GCGGGGATCC TGTTTAAAGC CCTGGATTTT GGCATTGATA TTTCCATTCA GGCAGGCACC AAATACCTGA TCGGCCATTC CGACGCCATG GTGGGCACCG CGGTGGCGAA CGCGCGCTGC TGGCCGCAGC TGCGTGAAAA TGCCTACCTG ATGGGGCAAA TGCTGGACGC CGATACTGCC TATATGACCA GCCGCGGCCT GCGCACCCTG GGCGTGCGCC TGCGTCAGCA TCATGAAAGC AGCCTGCGCA TCGCCGAGTG GCTGGCGCAG CACCCGCAGG TGGCGCGGGT AAACCACCCC GCCCTGCCCG GCAGTAAAGG TCATGAGTTC TGGAAACGTG ATTTCACCGG CAGCAGCGGG CTATTCTCGT TTGTGCTCAG CAAGCGCCTC AATGATGCCG AACTGGCGGA GTACCTCGAC AATTTCAGCC TGTTCAGTAT GGCCTATTCG TGGGGCGGCT TTGAGTCGCT GATTCTCGCC AACCAGCCGG AGCAAATCGC CCACATTCGA CCGGATGCTG AAGTCGACTT CAGCGGCACG CTGATCCGCC TGCACATTGG TCTCGAAAAT GTTGACGATC TGCAGGCGGA TTTAGCAGCG GGCTTCGCGC GTATCGTGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT