SSGCID: Target Page

KlpnC.00856.a

Ferrochelatase

CENTER ID:	KlpnC.00856.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00856.a.B1.GE44246	Full length(KlpnC.00856.a)		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.1168
UniProt:	A0A0H3GNR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHQTKTGILL ANLGTPDAPT PGAVKRYLRQ FLSDKRVVDT SRLLWWPLLR GVILPIRSPR VAKLYQSVWM EEGSPLMVYS RRQQQALAAR LPDTPVALGM SYGSPSLASA VDDLLAQGVE HIVVLPLYPQ YSCSTVAAVW DELARILAKK RAIPGISFIR DYAEHPDYIH ALAASVRASF AVHGEPDLLL LSYHGIPQRY ANQGDDYPQR CRDTTRELVS ALGLPPERVM MTFQSRFGRE PWLTPYTDET LKMLGEKGTK HIQVLCPGFA ADCLETLEEI AVQNREIFLE AGGKQYEYIP ALNADAAHIE MMVNLTAPYR

NT Sequence

ATGCATCAGA CGAAAACAGG TATCTTGCTG GCCAATTTAG GCACTCCCGA TGCGCCCACC CCCGGGGCGG TGAAGCGCTA TCTGCGACAA TTCCTCAGCG ACAAACGGGT GGTGGATACG TCTCGTCTGC TATGGTGGCC GCTGCTGCGC GGCGTCATAC TGCCGATTCG CTCCCCGCGC GTGGCGAAGC TCTATCAGTC CGTGTGGATG GAAGAGGGAT CGCCGCTGAT GGTGTATAGT CGTCGCCAGC AGCAGGCGCT GGCTGCCAGG CTGCCGGATA CCCCGGTGGC GCTGGGCATG AGCTATGGTT CACCGTCACT GGCCAGCGCG GTGGATGACT TACTGGCGCA GGGGGTGGAG CACATCGTGG TGCTGCCGCT GTATCCGCAA TATTCCTGCT CGACGGTGGC CGCCGTCTGG GACGAACTGG CGCGGATTCT GGCGAAAAAG CGCGCCATTC CGGGTATCTC GTTTATTCGC GACTATGCCG AACATCCGGA CTATATCCAT GCGCTGGCGG CCAGCGTGCG CGCTTCCTTC GCTGTCCATG GGGAACCGGA TCTGCTGCTG CTCTCCTATC ATGGGATCCC GCAGCGCTAT GCCAACCAGG GCGATGATTA TCCTCAGCGC TGTCGCGATA CGACGCGGGA ACTGGTTTCC GCGCTGGGGC TGCCGCCGGA GCGGGTGATG ATGACTTTCC AGTCGCGCTT TGGCCGGGAA CCCTGGCTGA CCCCGTACAC CGATGAAACG CTAAAGATGC TCGGCGAGAA AGGGACGAAG CATATTCAGG TGCTGTGCCC GGGGTTTGCC GCCGACTGTC TCGAAACGCT GGAAGAAATC GCCGTCCAGA ACCGGGAAAT ATTCCTCGAA GCAGGCGGCA AGCAATATGA ATATATTCCG GCGCTTAATG CGGACGCAGC GCATATTGAG ATGATGGTCA ATCTGACGGC GCCTTATCGC

Details for KlpnC.00856.a.B1.GE44246

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMH QTKTGILLAN LGTPDAPTPG AVKRYLRQFL SDKRVVDTSR LLWWPLLRGV ILPIRSPRVA KLYQSVWMEE GSPLMVYSRR QQQALAARLP DTPVALGMSY GSPSLASAVD DLLAQGVEHI VVLPLYPQYS CSTVAAVWDE LARILAKKRA IPGISFIRDY AEHPDYIHAL AASVRASFAV HGEPDLLLLS YHGIPQRYAN QGDDYPQRCR DTTRELVSAL GLPPERVMMT FQSRFGREPW LTPYTDETLK MLGEKGTKHI QVLCPGFAAD CLETLEEIAV QNREIFLEAG GKQYEYIPAL NADAAHIEMX XXXTAPYR

Validated NT Sequence

cacttagcga taaggcgccg tnnnnnnnnn natcatctca atatgcgctg cgtccgcatt aagcgccgga atatattcat attgcttgcc gcctgcttcg aggaatattt cccggttctg gacggcgatt tcttccagcg tttcgagaca gtcggcggca aaccccgggc acagcacctg aatatgcttc gtccctttct cgccgagcat ctttagcgtt tcatcggtgt acggcgtcag ccagggttcc cggccaaagc gcgactggaa agtcatcatc acccgctccg gcggcagccc cagcgcggaa accagttccc gcgtcgtatc gcgacagcgc tgaggataat catcgccctg gttggcatag cgctgcggga tcccatgata ggagagcagc agcagatccg gttccccatg gacagcgaag gaagcgcgca cgctggccgc cagcgcatgg atatagtccg gatgttcggc atagtcgcga ataaacgaga tacccggaat ggcgcgcttt ttcgccagaa tccgcgccag ttcgtcccag acggcggcca ccgtcgagca ggaatattgc ggatacagcg gcagcaccac gatgtgctcc accccctgcg ccagtaagtc atccaccgcg ctggccagtg acggtgaacc atagctcatg cccagcgcca ccggggtatc cggcagcctg gcagccagcg cctgctgctg gcgacgacta tacaccatca gcggcgatcc ctcttccatc cacacggact gatagagctt cgccacacgc ggggagcgaa tcggcagtat gacgccgcgc agcagcggcc accatagcag acgagacgta tccaccaccc gtttgtcgct gaggaattgt cgcagatagc gcttcaccgc cccgggggtg ggcgcatcgg gagtgcctaa attggccagc aagatacctg ttttcgtctg atgcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatc agacgaaaac aggtatcttg ctggccaatt taggcactcc cgatgcgccc acccccgggg cggtgaagcg ctatctgcga caattcctca gcgacaaacg ggtggtggat acgtctcgtc tgctatggtg gccgctgctg cgcggcgtca tactgccgat tcgctccccg cgcgtggcga agctctatca gtccgtgtgg atggaagagg gatcgccgct gatggtgtat agtcgtcgcc agcagcaggc gctggctgcc aggctgccgg ataccccggt ggcgctgggc atgagctatg gttcaccgtc actggccagc gcggtggatg acttactggc gcagggggtg gagcacatcg tggtgctgcc gctgtatccg caatattcct gctcgacggt ggccgccgtc tgggacgaac tggcgcggat tctggcgaaa aagcgcgcca ttccgggtat ctcgtttatt cgcgactatg ccgaacatcc ggactatatc catgcgctgg cggccagcgt gcgcgcttcc ttcgctgtcc atggggaacc ggatctgctg ctgctctcct atcatgggat cccgcagcgc tatgccaacc agggcgatga ttatcctcag cgctgtcgcg atacgacgcg ggaactggtt tccgcgctgg ggctgccgcc ggagcgggtg atgatgactt tccagtcgcg ctttggccgg gaaccctggc tgaccccgta caccgatgaa acgctaaaga tgctcggcga gaaagggacg aagcatattc aggtgctgtg cccggggttt gccgccgact gtctcgaaac gctggaagaa atcgccgtcc agaaccggga aatattcctc gaagcaggcg gcaagcaata tgaatatatt ccggcgctta atgcggacgc agcgcatatt gagatgatgg tcaatctgac ggcgccttat cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat