KlpnC.00771.a

Diaminopimelate decarboxylase

CENTER ID: KlpnC.00771.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00771.a.B1.GE44243 Full length(KlpnC.00771.a) 1 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00771.a.B1.PW38990 Full length(KlpnC.00771.a) 1 420
KlpnC.00771.a.B1.PW38991 Full length(KlpnC.00771.a) 1 420

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4216
UniProt: A0A0H3GSP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPHSLYATDT DLTADNLLRL PAEFGCPVWV YDAQIIRRQI AQLSQFDVVR FAQKACSNIH ILRLMREQGV KVDSVSLGEI ERALAAGYDP QQNPEDIVFT ADLIDDATLD RVKALQIPVN AGSIDMLSQL GEVSPGHRVW LRVNPGFGHG HSQKTNTGGE NSKHGIWYSH LPAALEVMRR YQLQLVGIHM HIGSGVDYGH LEQVCGAMVR QVVDFGQDLQ AISAGGGLSI PYREGEEAID TAHYYGLWNR AREQIAAHLG HPVKLEIEPG RFLVAESGVL VSQVRSVKEM GSRHFVLIDA GFNDLMRPAM YGSYHHITAL AGDGRDLSAA PQVETVVAGP LCESGDVFTQ QEGGKVETRL LPAVAPGDYL VLHDTGAYGA SMSSNYNSRP LLPEVLFDGG KARLIRRRQT IQELLALELI
NT Sequence
ATGCCACACT CGCTTTACGC CACCGATACT GACCTGACCG CAGACAACCT GCTGCGCCTG CCCGCGGAAT TTGGCTGTCC GGTCTGGGTC TATGATGCGC AGATTATTCG CCGCCAGATT GCCCAGCTCA GCCAGTTCGA CGTGGTGCGT TTTGCGCAAA AAGCCTGCTC TAACATTCAT ATTCTGCGTC TGATGCGTGA GCAGGGGGTG AAGGTGGACT CGGTGTCGCT GGGGGAGATC GAGCGGGCGC TGGCGGCAGG CTACGATCCG CAGCAGAATC CCGAGGATAT CGTCTTCACC GCCGATCTGA TCGACGACGC CACCCTCGAT CGGGTGAAGG CGTTACAGAT CCCGGTGAAC GCCGGCTCGA TCGACATGCT GAGCCAGCTG GGCGAGGTGT CGCCGGGCCA TCGCGTCTGG CTGCGCGTCA ACCCGGGCTT TGGCCACGGC CACAGCCAGA AAACCAATAC CGGCGGTGAG AATAGCAAGC ATGGCATCTG GTACAGCCAC CTGCCGGCGG CGCTGGAAGT GATGCGTCGC TATCAGCTGC AGCTGGTCGG GATCCATATG CATATCGGCT CTGGCGTTGA CTATGGTCAC CTGGAGCAGG TGTGCGGCGC GATGGTGCGC CAGGTCGTCG ACTTTGGCCA GGATCTGCAG GCCATCTCCG CCGGCGGCGG TCTGTCGATT CCTTACCGCG AAGGTGAAGA GGCCATCGAC ACCGCCCACT ATTATGGCCT GTGGAACCGC GCCCGGGAGC AGATCGCGGC CCATCTGGGG CACCCGGTGA AACTGGAGAT TGAGCCAGGA CGCTTCCTGG TGGCCGAGTC CGGCGTGCTG GTGTCGCAGG TGCGCAGTGT GAAAGAGATG GGCAGCCGTC ACTTCGTGCT GATTGACGCC GGCTTTAACG ACCTGATGCG CCCGGCAATG TACGGTAGCT ATCACCATAT CACCGCTCTG GCGGGGGATG GCCGGGATCT CAGCGCCGCG CCGCAGGTCG AAACCGTGGT GGCCGGGCCG CTGTGCGAAT CCGGGGATGT CTTTACCCAG CAGGAGGGGG GCAAAGTCGA GACCCGCCTG CTGCCGGCGG TGGCGCCTGG GGATTATCTG GTGCTCCATG ACACCGGCGC CTACGGGGCT TCGATGTCGT CCAACTATAA CAGCCGTCCG CTGCTGCCGG AGGTGCTGTT TGATGGCGGT AAGGCGCGGC TGATCCGCCG TCGCCAGACC ATCCAGGAGC TGCTGGCCTT AGAGCTTATT
Details for KlpnC.00771.a.B1.GE44243
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP HSLYATDTDL TADNLLRLPA EFGCPVWVYD AQIIRRQIAQ LSQFDVVRFA QKACSNIHIL RLMREQGVKV DSVSLGEIER ALAAGYDPQQ NPEDIVFTAD LIDDATLDRV KALQIPVNAG SIDMLSQLGE VSPGHRVWLR VNPGFGHGHS QKTNTGGENS KHGIWYSHLP AALEVMRRYQ LQLVGIHMHI GSGVDYGHLE QVCGAMVRQV VDFGQDLQAI SAGGGLSIPY REGEEAIDTA HYYGLWNRAR EQIAAHLGHP VKLEIEPGRF LVAESGVLVS QVRSVKEMGS RHFVLIDAGF NDLMRPAMYG SYHHITALAG DGRDLSAAPQ VETVVAGPLC ESGDVFTQQE GGKVETRLLP AVAPGDYLVL HDTGAYGASM SSNYNSRPLL PEVLFDGGKA RLIRRRQTI
Validated NT Sequence
tggatggtct ggcgacggcg gatcagccgc gccttgccgc catcaaacag cacctccggc agcagcggac ggctgttata gttggacgac atcgaagccc cgtaggcgcc ggtgtcatgg agcaccagat aatccccagg cgccactgcc ggcagcaggc gggtctcgac tttgcccccc tcctgctggg taaagacatc cccggattcg cacagcggcc cggccaccac ggtttcgacc tgcggcgcgg cgctgagatc ccggccatcc cccgccagag cggtgatatg gtgatagcta ccgtacattg ccgggcgcat caggtcgtta aagccggcgt caatcagcac gaagtgacgg ctgcccatct ctttcacgct gcgcacctgc gacaccagca cgccggactc ggccaccagg aagcgcccgg gctcaatctc cagtttcacc gggtgcccca gatgggccgc gatctgctcc cgggcgcggt tccacaggcc ataatagtgg gcggtgtcga tggcctcttc accttcgcgg taaggaatcg acagaccgcc gccggcggag atggcctgca gatcctggcc aaagtcgacg acctggcgca ccatcgcgcc gcacacctgc tccaggtgac catagtcaac gccagagccg atatgcatat ggatcccgac cagctgcagc tgatagcgac gcatcacttc cagcgccgcc ggcaggtggc tgtaccagat gccatgcttg ctattctcac cgccggtatt ggttttctgg ctgtggccgt ggccaaagcc cgggttgacg cgcagccaga cgcgatggcc cggcgacacc tcgcccagct ggctcagcat gtcgatcgag ccggcgttca ccgggatctg taacgccttc acccgatcga gggtggcgtc gtcgatcaga tcggcggtga agacgatatc ctcgggattc tgctgcggat cgtagcctgc cgccagcgcc cgctcgatct cccccagcga caccgagtcc accttcaccc cctgctcacg catcagacgc agaatatgaa tgttagagca ggctttttgc gcaaaacgca ccacgtcgaa ctggctgagc tgggctatct ggcggcgaat aatctgcgca tcatagaccc agaccgggca gccaaattcc gccggcaggc gcagcaggtt gtccgcggtc aggtcagtat cggtggcgta aagcgagtgt ggcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMP HSLYATDTDL TADNLLRLPA EFGCPVWVYD AQIIRRQIAQ LSQFDVVRFA QKACSNIHIL RLMREQGVKV DSVSLGEIER ALAAGYDPQQ NPEDIVFTAD LIDDATLDRV KALQIPVNAG SIDMLSQLGE VSPGHRVWLR VNPGFGHGHS QKTNTGGENS KHGIWYSHLP AALEVMRRYQ LQLVGIHMHI GSGVDYGHLE QVCGAMVRQV VDFGQDLQAI SAGGGLSIPY REGEEAIDTA HYYGLWNRAR EQIAAHLGHP VKLEIEPGRF LVAESGVLVS QVRSVKEMGS RHFVLIDAGF NDLMRPAMYG SYHHITALAG DGRDLSAAPQ VETVVAGPLC ESGDVFTQQE GGKVETRLLP AVAPGDYLVL HDTGAYGASM SSNYNSRPLL PEVLFDGGKA RLIRRRQTIQ ELLALELI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccac actcgcttta cgccaccgat actgacctga ccgcagacaa cctgctgcgc ctgcccgcgg aatttggctg tccggtctgg gtctatgatg cgcagattat tcgccgccag attgcccagc tcagccagtt cgacgtggtg cgttttgcgc aaaaagcctg ctctaacatt catattctgc gtctgatgcg tgagcagggg gtgaaggtgg actcggtgtc gctgggggag atcgagcggg cgctggcggc aggctacgat ccgcagcaga atcccgagga tatcgtcttc accgccgatc tgatcgacga cgccaccctc gatcgggtga aggcgttaca gatcccggtg aacgccggct cgatcgacat gctgagccag ctgggcgagg tgtcgccggg ccatcgcgtc tggctgcgcg tcaacccggg ctttggccac ggccacagcc agaaaaccaa taccggcggt gagaatagca agcatggcat ctggtacagc cacctgccgg cggcgctgga agtgatgcgt cgctatcagc tgcagctggt cgggatccat atgcatatcg gctctggcgt tgactatggt cacctggagc aggtgtgcgg cgcgatggtg cgccaggtcg tcgactttgg ccaggatctg caggccatct ccgccggcgg cggtctgtcg attccttacc gcgaaggtga agaggccatc gacaccgccc actattatgg cctgtggaac cgcgcccggg agcagatcgc ggcccatctg gggcacccgg tgaaactgga gattgagcca ggacgcttcc tggtggccga gtccggcgtg ctggtgtcgc aggtgcgcag tgtgaaagag atgggcagcc gtcacttcgt gctgattgac gccggcttta acgacctgat gcgcccggca atgtacggta gctatcacca tatcaccgct ctggcggggg atggccggga tctcagcgcc gcgccgcagg tcgaaaccgt ggtggccggg ccgctgtgcg aatccgggga tgtctttacc cagcaggagg ggggcaaagt cgagacccgc ctgctgccgg cggtggcgcc tggggattat ctggtgctcc atgacaccgg cgcctacggg gcttcgatgt cgtccaacta taacagccgt ccgctgctgc cggaggtgct gtttgatggc ggtaaggcgc ggctgatccg ccgtcgccag accatccagg agctgctggc cttagagctt atttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for KlpnC.00771.a.B1.PW38990
PURIFICATION DATe: 5/9/2021
CONCENTRATION: 20mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP HSLYATDTDL TADNLLRLPA EFGCPVWVYD AQIIRRQIAQ LSQFDVVRFA QKACSNIHIL RLMREQGVKV DSVSLGEIER ALAAGYDPQQ NPEDIVFTAD LIDDATLDRV KALQIPVNAG SIDMLSQLGE VSPGHRVWLR VNPGFGHGHS QKTNTGGENS KHGIWYSHLP AALEVMRRYQ LQLVGIHMHI GSGVDYGHLE QVCGAMVRQV VDFGQDLQAI SAGGGLSIPY REGEEAIDTA HYYGLWNRAR EQIAAHLGHP VKLEIEPGRF LVAESGVLVS QVRSVKEMGS RHFVLIDAGF NDLMRPAMYG SYHHITALAG DGRDLSAAPQ VETVVAGPLC ESGDVFTQQE GGKVETRLLP AVAPGDYLVL HDTGAYGASM SSNYNSRPLL PEVLFDGGKA RLIRRRQTI
Validated NT Sequence
tggatggtct ggcgacggcg gatcagccgc gccttgccgc catcaaacag cacctccggc agcagcggac ggctgttata gttggacgac atcgaagccc cgtaggcgcc ggtgtcatgg agcaccagat aatccccagg cgccactgcc ggcagcaggc gggtctcgac tttgcccccc tcctgctggg taaagacatc cccggattcg cacagcggcc cggccaccac ggtttcgacc tgcggcgcgg cgctgagatc ccggccatcc cccgccagag cggtgatatg gtgatagcta ccgtacattg ccgggcgcat caggtcgtta aagccggcgt caatcagcac gaagtgacgg ctgcccatct ctttcacgct gcgcacctgc gacaccagca cgccggactc ggccaccagg aagcgcccgg gctcaatctc cagtttcacc gggtgcccca gatgggccgc gatctgctcc cgggcgcggt tccacaggcc ataatagtgg gcggtgtcga tggcctcttc accttcgcgg taaggaatcg acagaccgcc gccggcggag atggcctgca gatcctggcc aaagtcgacg acctggcgca ccatcgcgcc gcacacctgc tccaggtgac catagtcaac gccagagccg atatgcatat ggatcccgac cagctgcagc tgatagcgac gcatcacttc cagcgccgcc ggcaggtggc tgtaccagat gccatgcttg ctattctcac cgccggtatt ggttttctgg ctgtggccgt ggccaaagcc cgggttgacg cgcagccaga cgcgatggcc cggcgacacc tcgcccagct ggctcagcat gtcgatcgag ccggcgttca ccgggatctg taacgccttc acccgatcga gggtggcgtc gtcgatcaga tcggcggtga agacgatatc ctcgggattc tgctgcggat cgtagcctgc cgccagcgcc cgctcgatct cccccagcga caccgagtcc accttcaccc cctgctcacg catcagacgc agaatatgaa tgttagagca ggctttttgc gcaaaacgca ccacgtcgaa ctggctgagc tgggctatct ggcggcgaat aatctgcgca tcatagaccc agaccgggca gccaaattcc gccggcaggc gcagcaggtt gtccgcggtc aggtcagtat cggtggcgta aagcgagtgt ggcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMP HSLYATDTDL TADNLLRLPA EFGCPVWVYD AQIIRRQIAQ LSQFDVVRFA QKACSNIHIL RLMREQGVKV DSVSLGEIER ALAAGYDPQQ NPEDIVFTAD LIDDATLDRV KALQIPVNAG SIDMLSQLGE VSPGHRVWLR VNPGFGHGHS QKTNTGGENS KHGIWYSHLP AALEVMRRYQ LQLVGIHMHI GSGVDYGHLE QVCGAMVRQV VDFGQDLQAI SAGGGLSIPY REGEEAIDTA HYYGLWNRAR EQIAAHLGHP VKLEIEPGRF LVAESGVLVS QVRSVKEMGS RHFVLIDAGF NDLMRPAMYG SYHHITALAG DGRDLSAAPQ VETVVAGPLC ESGDVFTQQE GGKVETRLLP AVAPGDYLVL HDTGAYGASM SSNYNSRPLL PEVLFDGGKA RLIRRRQTIQ ELLALELI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAC ACTCGCTTTA CGCCACCGAT ACTGACCTGA CCGCAGACAA CCTGCTGCGC CTGCCCGCGG AATTTGGCTG TCCGGTCTGG GTCTATGATG CGCAGATTAT TCGCCGCCAG ATTGCCCAGC TCAGCCAGTT CGACGTGGTG CGTTTTGCGC AAAAAGCCTG CTCTAACATT CATATTCTGC GTCTGATGCG TGAGCAGGGG GTGAAGGTGG ACTCGGTGTC GCTGGGGGAG ATCGAGCGGG CGCTGGCGGC AGGCTACGAT CCGCAGCAGA ATCCCGAGGA TATCGTCTTC ACCGCCGATC TGATCGACGA CGCCACCCTC GATCGGGTGA AGGCGTTACA GATCCCGGTG AACGCCGGCT CGATCGACAT GCTGAGCCAG CTGGGCGAGG TGTCGCCGGG CCATCGCGTC TGGCTGCGCG TCAACCCGGG CTTTGGCCAC GGCCACAGCC AGAAAACCAA TACCGGCGGT GAGAATAGCA AGCATGGCAT CTGGTACAGC CACCTGCCGG CGGCGCTGGA AGTGATGCGT CGCTATCAGC TGCAGCTGGT CGGGATCCAT ATGCATATCG GCTCTGGCGT TGACTATGGT CACCTGGAGC AGGTGTGCGG CGCGATGGTG CGCCAGGTCG TCGACTTTGG CCAGGATCTG CAGGCCATCT CCGCCGGCGG CGGTCTGTCG ATTCCTTACC GCGAAGGTGA AGAGGCCATC GACACCGCCC ACTATTATGG CCTGTGGAAC CGCGCCCGGG AGCAGATCGC GGCCCATCTG GGGCACCCGG TGAAACTGGA GATTGAGCCA GGACGCTTCC TGGTGGCCGA GTCCGGCGTG CTGGTGTCGC AGGTGCGCAG TGTGAAAGAG ATGGGCAGCC GTCACTTCGT GCTGATTGAC GCCGGCTTTA ACGACCTGAT GCGCCCGGCA ATGTACGGTA GCTATCACCA TATCACCGCT CTGGCGGGGG ATGGCCGGGA TCTCAGCGCC GCGCCGCAGG TCGAAACCGT GGTGGCCGGG CCGCTGTGCG AATCCGGGGA TGTCTTTACC CAGCAGGAGG GGGGCAAAGT CGAGACCCGC CTGCTGCCGG CGGTGGCGCC TGGGGATTAT CTGGTGCTCC ATGACACCGG CGCCTACGGG GCTTCGATGT CGTCCAACTA TAACAGCCGT CCGCTGCTGC CGGAGGTGCT GTTTGATGGC GGTAAGGCGC GGCTGATCCG CCGTCGCCAG ACCATCCAGG AGCTGCTGGC CTTAGAGCTT ATTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for KlpnC.00771.a.B1.PW38991
PURIFICATION DATe: 5/9/2021
CONCENTRATION: 7mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP HSLYATDTDL TADNLLRLPA EFGCPVWVYD AQIIRRQIAQ LSQFDVVRFA QKACSNIHIL RLMREQGVKV DSVSLGEIER ALAAGYDPQQ NPEDIVFTAD LIDDATLDRV KALQIPVNAG SIDMLSQLGE VSPGHRVWLR VNPGFGHGHS QKTNTGGENS KHGIWYSHLP AALEVMRRYQ LQLVGIHMHI GSGVDYGHLE QVCGAMVRQV VDFGQDLQAI SAGGGLSIPY REGEEAIDTA HYYGLWNRAR EQIAAHLGHP VKLEIEPGRF LVAESGVLVS QVRSVKEMGS RHFVLIDAGF NDLMRPAMYG SYHHITALAG DGRDLSAAPQ VETVVAGPLC ESGDVFTQQE GGKVETRLLP AVAPGDYLVL HDTGAYGASM SSNYNSRPLL PEVLFDGGKA RLIRRRQTI
Validated NT Sequence
tggatggtct ggcgacggcg gatcagccgc gccttgccgc catcaaacag cacctccggc agcagcggac ggctgttata gttggacgac atcgaagccc cgtaggcgcc ggtgtcatgg agcaccagat aatccccagg cgccactgcc ggcagcaggc gggtctcgac tttgcccccc tcctgctggg taaagacatc cccggattcg cacagcggcc cggccaccac ggtttcgacc tgcggcgcgg cgctgagatc ccggccatcc cccgccagag cggtgatatg gtgatagcta ccgtacattg ccgggcgcat caggtcgtta aagccggcgt caatcagcac gaagtgacgg ctgcccatct ctttcacgct gcgcacctgc gacaccagca cgccggactc ggccaccagg aagcgcccgg gctcaatctc cagtttcacc gggtgcccca gatgggccgc gatctgctcc cgggcgcggt tccacaggcc ataatagtgg gcggtgtcga tggcctcttc accttcgcgg taaggaatcg acagaccgcc gccggcggag atggcctgca gatcctggcc aaagtcgacg acctggcgca ccatcgcgcc gcacacctgc tccaggtgac catagtcaac gccagagccg atatgcatat ggatcccgac cagctgcagc tgatagcgac gcatcacttc cagcgccgcc ggcaggtggc tgtaccagat gccatgcttg ctattctcac cgccggtatt ggttttctgg ctgtggccgt ggccaaagcc cgggttgacg cgcagccaga cgcgatggcc cggcgacacc tcgcccagct ggctcagcat gtcgatcgag ccggcgttca ccgggatctg taacgccttc acccgatcga gggtggcgtc gtcgatcaga tcggcggtga agacgatatc ctcgggattc tgctgcggat cgtagcctgc cgccagcgcc cgctcgatct cccccagcga caccgagtcc accttcaccc cctgctcacg catcagacgc agaatatgaa tgttagagca ggctttttgc gcaaaacgca ccacgtcgaa ctggctgagc tgggctatct ggcggcgaat aatctgcgca tcatagaccc agaccgggca gccaaattcc gccggcaggc gcagcaggtt gtccgcggtc aggtcagtat cggtggcgta aagcgagtgt ggcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMP HSLYATDTDL TADNLLRLPA EFGCPVWVYD AQIIRRQIAQ LSQFDVVRFA QKACSNIHIL RLMREQGVKV DSVSLGEIER ALAAGYDPQQ NPEDIVFTAD LIDDATLDRV KALQIPVNAG SIDMLSQLGE VSPGHRVWLR VNPGFGHGHS QKTNTGGENS KHGIWYSHLP AALEVMRRYQ LQLVGIHMHI GSGVDYGHLE QVCGAMVRQV VDFGQDLQAI SAGGGLSIPY REGEEAIDTA HYYGLWNRAR EQIAAHLGHP VKLEIEPGRF LVAESGVLVS QVRSVKEMGS RHFVLIDAGF NDLMRPAMYG SYHHITALAG DGRDLSAAPQ VETVVAGPLC ESGDVFTQQE GGKVETRLLP AVAPGDYLVL HDTGAYGASM SSNYNSRPLL PEVLFDGGKA RLIRRRQTIQ ELLALELI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAC ACTCGCTTTA CGCCACCGAT ACTGACCTGA CCGCAGACAA CCTGCTGCGC CTGCCCGCGG AATTTGGCTG TCCGGTCTGG GTCTATGATG CGCAGATTAT TCGCCGCCAG ATTGCCCAGC TCAGCCAGTT CGACGTGGTG CGTTTTGCGC AAAAAGCCTG CTCTAACATT CATATTCTGC GTCTGATGCG TGAGCAGGGG GTGAAGGTGG ACTCGGTGTC GCTGGGGGAG ATCGAGCGGG CGCTGGCGGC AGGCTACGAT CCGCAGCAGA ATCCCGAGGA TATCGTCTTC ACCGCCGATC TGATCGACGA CGCCACCCTC GATCGGGTGA AGGCGTTACA GATCCCGGTG AACGCCGGCT CGATCGACAT GCTGAGCCAG CTGGGCGAGG TGTCGCCGGG CCATCGCGTC TGGCTGCGCG TCAACCCGGG CTTTGGCCAC GGCCACAGCC AGAAAACCAA TACCGGCGGT GAGAATAGCA AGCATGGCAT CTGGTACAGC CACCTGCCGG CGGCGCTGGA AGTGATGCGT CGCTATCAGC TGCAGCTGGT CGGGATCCAT ATGCATATCG GCTCTGGCGT TGACTATGGT CACCTGGAGC AGGTGTGCGG CGCGATGGTG CGCCAGGTCG TCGACTTTGG CCAGGATCTG CAGGCCATCT CCGCCGGCGG CGGTCTGTCG ATTCCTTACC GCGAAGGTGA AGAGGCCATC GACACCGCCC ACTATTATGG CCTGTGGAAC CGCGCCCGGG AGCAGATCGC GGCCCATCTG GGGCACCCGG TGAAACTGGA GATTGAGCCA GGACGCTTCC TGGTGGCCGA GTCCGGCGTG CTGGTGTCGC AGGTGCGCAG TGTGAAAGAG ATGGGCAGCC GTCACTTCGT GCTGATTGAC GCCGGCTTTA ACGACCTGAT GCGCCCGGCA ATGTACGGTA GCTATCACCA TATCACCGCT CTGGCGGGGG ATGGCCGGGA TCTCAGCGCC GCGCCGCAGG TCGAAACCGT GGTGGCCGGG CCGCTGTGCG AATCCGGGGA TGTCTTTACC CAGCAGGAGG GGGGCAAAGT CGAGACCCGC CTGCTGCCGG CGGTGGCGCC TGGGGATTAT CTGGTGCTCC ATGACACCGG CGCCTACGGG GCTTCGATGT CGTCCAACTA TAACAGCCGT CCGCTGCTGC CGGAGGTGCT GTTTGATGGC GGTAAGGCGC GGCTGATCCG CCGTCGCCAG ACCATCCAGG AGCTGCTGGC CTTAGAGCTT ATTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT