KlpnC.00759.a

3'(2'),5'-bisphosphate nucleotidase CysQ

CENTER ID: KlpnC.00759.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00759.a.B1.GE44241 Full length(KlpnC.00759.a) 1 231
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.444
UniProt: A0A0H3GLJ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEVYDGNQPI NVASKKDDSP VTAADIAAHK VIVSGLQALD PDTPILSEED PPSWEVRQHW QRYWLVDPLD GTKEFIKRNG EFTVNIALIE NGKPTLGVVY APVMKVMYSA QNGKAWKEEC GVRKQIQVRD ARPPLVVISR SHSNDPELQE YLEQLGEHQT TSIGSSLKFC LVAEGQAQLY PRFGPTSTWD TAAGHAVAVA AGAHVHDWQG KTLDYTPRES FLNPGFRVSI Y
NT Sequence
ATGGAAGTCT ACGATGGAAA CCAGCCGATC AACGTCGCCA GCAAGAAAGA TGATTCTCCG GTCACGGCGG CGGATATCGC AGCGCATAAA GTCATTGTCA GCGGCCTGCA GGCGCTGGAT CCGGATACCC CGATCCTCTC CGAGGAGGAC CCGCCGTCCT GGGAAGTGCG CCAGCACTGG CAGCGCTACT GGTTGGTCGA CCCGCTGGAC GGCACCAAAG AGTTTATTAA GCGTAACGGC GAATTCACCG TCAATATCGC GCTGATTGAA AACGGCAAGC CGACGCTGGG CGTTGTCTAT GCGCCGGTGA TGAAGGTGAT GTACAGCGCG CAAAATGGCA AAGCCTGGAA AGAAGAGTGC GGCGTACGCA AGCAGATCCA GGTCCGCGAT GCCCGTCCGC CGCTGGTGGT GATTAGCCGC TCCCATAGCA ACGATCCGGA GCTGCAGGAG TATCTTGAGC AACTTGGCGA ACACCAGACC ACCTCAATCG GTTCGTCGCT GAAATTCTGC CTGGTGGCGG AAGGTCAGGC CCAGCTCTAT CCGCGCTTCG GGCCGACCAG CACCTGGGAC ACCGCCGCCG GCCATGCGGT CGCGGTGGCC GCCGGGGCGC ATGTTCACGA CTGGCAGGGG AAAACCCTCG ACTACACGCC GCGTGAATCC TTCCTTAACC CCGGCTTCCG GGTCTCTATT TAT
Details for KlpnC.00759.a.B1.GE44241
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME VYDGNQPINV ASKKDDSPVT AADIAAHKVI VSGLQALDPD TPILSEEDPP AWEVRQHWQR YWLVDPLDGT KEFIKRNGEF TVNIALIENG KPTLGVVYAP VMKVMYSAQN GKAWKEECGV RKQIQVRDAR PPLVVISRSH SNDPELQEYL EQLGEHQTTS IGSSLKFCLV AEGQAQLYPR FGPTSTWDTA AGHAVAVAAG AHVHDWQGKT LDYTPRESFL NPGFRVSIY
Validated NT Sequence
atcttactca cttaataaat agagacccgg aagccggggt tgaggaagga ttcacgcggc gtgtagtcga gggttttccc ctgccagtcg tgaacatgcg ccccggcggc caccgcgacc gcatggccgg cggcagtgtc ccaggtgctg gtcggcccga agcgcggata gagctgggcc tgaccttccg ccaccaggca gaatttcagc gacgaaccga ttgaggtggt ctggtgttcg ccaagttgct caaggtactc ctgcagctcc ggatcgttgc tgtgggagcg gctaatcacc accagcggcg gacgggcatc gcggacctgg atctgcttgc gtacgccgca ctcttctttc caggctttgc cattttgcgc gctgtacatc accttcatca ccggcgcata gacaacgccc agcgtcggct tgccgttttc aatcagcgcg atattgacgg tgaattcgcc gttacgctta ataaactctt tggtgccgtc cagcgggtcg accagccagt agcgctgcca gtgctggcgc acttcccacg ccggcgggtc ctcctcggag aggatcgggg tatccggatc cagcgcctgc aggccgctga caatgacttt atgcgctgcg atatccgccg ccgtgaccgg agaatcatct ttcttgctgg cgacgttgat cggctggttt ccatcgtaga cttccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHME VYDGNQPINV ASKKDDSPVT AADIAAHKVI VSGLQALDPD TPILSEEDPP SWEVRQHWQR YWLVDPLDGT KEFIKRNGEF TVNIALIENG KPTLGVVYAP VMKVMYSAQN GKAWKEECGV RKQIQVRDAR PPLVVISRSH SNDPELQEYL EQLGEHQTTS IGSSLKFCLV AEGQAQLYPR FGPTSTWDTA AGHAVAVAAG AHVHDWQGKT LDYTPRESFL NPGFRVSIY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag tctacgatgg aaaccagccg atcaacgtcg ccagcaagaa agatgattct ccggtcacgg cggcggatat cgcagcgcat aaagtcattg tcagcggcct gcaggcgctg gatccggata ccccgatcct ctccgaggag gacccgccgt cctgggaagt gcgccagcac tggcagcgct actggttggt cgacccgctg gacggcacca aagagtttat taagcgtaac ggcgaattca ccgtcaatat cgcgctgatt gaaaacggca agccgacgct gggcgttgtc tatgcgccgg tgatgaaggt gatgtacagc gcgcaaaatg gcaaagcctg gaaagaagag tgcggcgtac gcaagcagat ccaggtccgc gatgcccgtc cgccgctggt ggtgattagc cgctcccata gcaacgatcc ggagctgcag gagtatcttg agcaacttgg cgaacaccag accacctcaa tcggttcgtc gctgaaattc tgcctggtgg cggaaggtca ggcccagctc tatccgcgct tcgggccgac cagcacctgg gacaccgccg ccggccatgc ggtcgcggtg gccgccgggg cgcatgttca cgactggcag gggaaaaccc tcgactacac gccgcgtgaa tccttcctta accccggctt ccgggtctct atttattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t