KlpnC.00629.c

Enterobactin synthase subunit E

CENTER ID: KlpnC.00629.c
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00629.c.B1.GE44236 Full length(KlpnC.00629.c) 1 535
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.1407
UniProt: A0A0H3GJV2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIAFTRWPEE FAARYRQKGY WQDLPLTNLI TRHADNDAVA IIDGERQISY RQFNQLVDNL ACSLQRQGLK RGETALVQLG NVAEFYMTFF ALLRIGVAPV NALFSHQRSE LNAYAAQIKP ALLIADREHA LFADDSFLHA FIAEHPSLRV ALLRNDGGER DLATEINRPA DNFIANPTPA DEVAFFQLSG GSTGTPKLIP RTHNDYDYSI RRSNEICGIN AETRYLNALP AAHNYAMSSP GSLGVFLAGG RVILAADPSA TLCFPLIEKH QINVASLVPP AVSLWLQAIH EWGSNAQLQS LQLLQVGGAR LSATLAARIP AEIGCQLQQV FGMAEGLVNY TRLNDSPERI INTQGCPMCP DDEVWVADAD GNPLPRGEVG RLMTRGPYTF RGYYNSPQHN AEAFDADGFY CSGDLISIDE DGYITVQGRE KDQINRGGEK IAAEEIENLL LRHEAVIHAA LVSIEDNLLG EKSCAYLVVT SPLRAVAVRR FLREQGVAEF KLPDRVECVA ALPLTPVGKV DKKQLRQWLA EGKLG
NT Sequence
ATGATTGCAT TTACCCGTTG GCCGGAAGAG TTTGCGGCCC GCTATCGTCA AAAAGGCTAC TGGCAGGATC TGCCGTTAAC CAATCTGATC ACCCGTCACG CGGACAATGA CGCCGTGGCG ATTATCGATG GCGAGCGGCA GATTAGCTAC CGCCAGTTTA ACCAGCTGGT GGACAACCTC GCCTGCTCGC TGCAGCGCCA GGGACTGAAG CGCGGGGAGA CCGCGCTGGT GCAGTTGGGC AATGTCGCCG AGTTTTACAT GACCTTCTTT GCGCTGCTGC GCATCGGCGT CGCGCCAGTG AACGCTTTGT TCAGCCACCA GCGCAGCGAA CTTAACGCCT ACGCCGCGCA GATTAAACCG GCGCTGCTGA TTGCCGATCG CGAGCACGCT CTGTTTGCTG ACGATAGCTT TCTCCACGCG TTTATCGCGG AACATCCTTC CCTGCGCGTC GCACTGTTGC GTAACGACGG CGGCGAGCGC GATCTGGCGA CGGAGATTAA CCGCCCGGCG GATAACTTTA TCGCCAATCC AACCCCTGCC GACGAAGTGG CCTTTTTCCA GCTCTCCGGC GGCAGCACCG GGACGCCGAA GCTTATCCCA CGCACCCATA ACGATTACGA CTACAGTATC CGCCGCAGCA ACGAGATCTG TGGGATTAAC GCTGAAACGC GCTACCTCAA CGCGCTGCCG GCGGCGCATA ACTATGCGAT GAGTTCCCCG GGTTCGCTCG GCGTCTTCCT CGCCGGCGGG CGGGTGATCC TCGCCGCTGA CCCGAGCGCC ACGCTCTGTT TCCCGCTGAT CGAAAAACAT CAGATCAACG TTGCCTCGCT GGTGCCGCCG GCAGTCAGCC TGTGGCTGCA GGCGATTCAC GAATGGGGCA GCAACGCCCA GCTGCAATCG CTGCAGCTGC TGCAGGTCGG CGGCGCGCGC CTGTCAGCGA CGCTCGCCGC GCGCATTCCG GCGGAAATCG GCTGTCAGCT GCAGCAGGTG TTCGGCATGG CGGAAGGGCT GGTGAATTAC ACCCGCCTCA ACGATAGCCC GGAGCGGATT ATCAACACCC AGGGTTGCCC GATGTGTCCG GACGACGAAG TGTGGGTGGC GGACGCCGAC GGCAACCCGC TGCCGCGCGG CGAAGTTGGC CGTCTGATGA CCCGCGGTCC TTATACCTTC CGCGGCTATT ACAACAGTCC GCAACATAAC GCTGAAGCCT TTGATGCCGA TGGATTTTAC TGCTCCGGTG ATCTGATTTC GATCGATGAA GATGGCTATA TCACCGTCCA GGGCCGGGAA AAAGATCAGA TCAACCGCGG CGGCGAGAAG ATCGCCGCTG AAGAGATCGA AAACCTGCTG CTGCGCCATG AGGCGGTGAT CCACGCCGCG CTGGTCAGCA TCGAAGACAA TCTGCTGGGC GAGAAAAGCT GCGCTTATCT GGTGGTGACC TCGCCGCTGC GCGCCGTCGC CGTGCGCCGC TTCCTGCGCG AGCAGGGGGT GGCGGAATTT AAACTGCCGG ACCGCGTGGA GTGCGTTGCC GCGCTGCCGC TGACGCCGGT GGGCAAAGTC GATAAAAAAC AATTACGTCA GTGGCTGGCC GAAGGCAAGC TGGGC
Details for KlpnC.00629.c.B1.GE44236
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 96
Validated AA Sequence
MIAFTRWPEE FAARYRQKGY WQDLPLTNLI TRHADNDAVA IIDGERQISY RQFNQLVDNL ACSLQRQGLK RGETALVQLG NVAEFYMTFF ALLRIGVAPV NALFNHQRSE LNAYAAQIKP ALLIADREHA LFADDSFLHA FIAEHPSLRV ALLRNDGGER DLATEINRPA DNFIAXPTPA DEVXFFQLSG GSTGTPKLIP RTHNDYDYSI RRSNEXCGIN AETRYLNALP AAHNYAMSSP GSLGVFLAGG RVILAADPSA XLCFPLIEKH QINVASLVPP AVSLWLQAIH EWGSNAQLQS LQLLQVGGAR LSATLAARIP XEIGCQLQQV FGMAEGLVNY TRLNDSPERI INTQGCPMCP DDEVWVADAD GNPLPRGEVG RLMTRGPYTF RGYYNSPQHN AEAFDAEGFY CSGDLISIDE DGYITVQGRE KDQINRGGEK IAAEEIENLL LRHEAVIHAA LVSIEDNLLG EKSCAYLVVT SPLRAVAVRR FLREQGVAEF KLPDRVECVA ALPLTPVGKV DKKQLR
Validated NT Sequence
gncgtaattg ttttttatcg actttgccca ccggcgtcag cggcagcgcg gcaacgcact ccacgcggtc cggcagttta aattccgcca ccccctgctc gcgcaggaag cggcgcacgg cgacggcgcg cagcggcgag gtcaccacca gataagcgca gcttttctcg cccagcagat tgtcttcgat gctgaccagc gcggcgtgga tcaccgcctc atggcgcagc agcaggtttt cgatctcttc agcggcgatc ttctcgccgc cgcggttgat ctgatctttt tcccggccct ggacggtgat atacccatct tcgtcgatcg aaatcagatc gccggagcaa taaaacccct cggcgtcaaa ggcttccgcg ttatgctgcg ggctgttgta gtagccgcgg aaagtgtacg gcccgcgggt catcaggcgg cccacttcgc cgcgcggcag cgggttgccg tcggcatccg ctacccacac ttcatcgtcc gggcacatcg gacagccctg ggtattgatg atccgctccg ggctatcgtt aaggcgggtg taattcacca gcccctccgc catgccgaac acctgctgca gctggcagcc gatttccncc ggaatgcgcg cggcgagggt cgctgacagg cgcgcgccgc cgacctgcag cagctgcagc gattgcagct gggcgttgct gccccattcg tgaatcgcct gcagccacag gctgaccgcc ggcggcacca gcgacgcgac gttaatctga tgtttttcga tcagcgggaa acagagcgng gcgctcgggt cagcggcgag gatcacccgc ccgccggcga ggaagacgcc gagcgaaccc ggggaactca tcgcatagtt atgcgccgcn ggcagcgcgt tgaggtagcg cgtttcagcg ttaatcccgc agntctcgtt gctgcggcgg atgctgtagt cgtaatcgtt atgggtgcga gggataagct tcggcgtccc ggtgctgccg ccggagagct ggaaaaagnc cacttcgtcg gcaggggttg gatnggcgat aaagttatcc gccgggcggt taatctccgt cgccagatcg cgctcgccgc cgtcgttacg caacagtgcg acgcgcaggg aaggatgttc cgcgataaac gcgtggagaa agctatcgtc agcaaacaga gcgtgctcgc gatcggcaat cagcagcgcc ggtttaatct gcgcggcgta ggcgttaagt tcgctgcgct ggtggttgaa caaagcgttc actggcgcga cgccgatgcg cagcagcgca aagaaggtca tgtaaaactc ggcgacattg cccagctgca ccagcgcggt ctccccgcgc ttcagtccct ggcgctgcag cgagcaggcg aggttgtcca ccagctggtt aaactggcgg tagctaatct gccgctcgcc atcgataatc gccacggcgt cattgtccgc gtgacgggtg atcagattgg ttaacggcag atcctgccag tagccttttt gacgatagcg ggccgcaaac tcttccggcc aacgggtaaa tgcaatcata tggtggnggn g
Expected Protein Sequence
MAHHHHHHMI AFTRWPEEFA ARYRQKGYWQ DLPLTNLITR HADNDAVAII DGERQISYRQ FNQLVDNLAC SLQRQGLKRG ETALVQLGNV AEFYMTFFAL LRIGVAPVNA LFSHQRSELN AYAAQIKPAL LIADREHALF ADDSFLHAFI AEHPSLRVAL LRNDGGERDL ATEINRPADN FIANPTPADE VAFFQLSGGS TGTPKLIPRT HNDYDYSIRR SNEICGINAE TRYLNALPAA HNYAMSSPGS LGVFLAGGRV ILAADPSATL CFPLIEKHQI NVASLVPPAV SLWLQAIHEW GSNAQLQSLQ LLQVGGARLS ATLAARIPAE IGCQLQQVFG MAEGLVNYTR LNDSPERIIN TQGCPMCPDD EVWVADADGN PLPRGEVGRL MTRGPYTFRG YYNSPQHNAE AFDADGFYCS GDLISIDEDG YITVQGREKD QINRGGEKIA AEEIENLLLR HEAVIHAALV SIEDNLLGEK SCAYLVVTSP LRAVAVRRFL REQGVAEFKL PDRVECVAAL PLTPVGKVDK KQLRQWLAEG KLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg catttacccg ttggccggaa gagtttgcgg cccgctatcg tcaaaaaggc tactggcagg atctgccgtt aaccaatctg atcacccgtc acgcggacaa tgacgccgtg gcgattatcg atggcgagcg gcagattagc taccgccagt ttaaccagct ggtggacaac ctcgcctgct cgctgcagcg ccagggactg aagcgcgggg agaccgcgct ggtgcagttg ggcaatgtcg ccgagtttta catgaccttc tttgcgctgc tgcgcatcgg cgtcgcgcca gtgaacgctt tgttcagcca ccagcgcagc gaacttaacg cctacgccgc gcagattaaa ccggcgctgc tgattgccga tcgcgagcac gctctgtttg ctgacgatag ctttctccac gcgtttatcg cggaacatcc ttccctgcgc gtcgcactgt tgcgtaacga cggcggcgag cgcgatctgg cgacggagat taaccgcccg gcggataact ttatcgccaa tccaacccct gccgacgaag tggccttttt ccagctctcc ggcggcagca ccgggacgcc gaagcttatc ccacgcaccc ataacgatta cgactacagt atccgccgca gcaacgagat ctgtgggatt aacgctgaaa cgcgctacct caacgcgctg ccggcggcgc ataactatgc gatgagttcc ccgggttcgc tcggcgtctt cctcgccggc gggcgggtga tcctcgccgc tgacccgagc gccacgctct gtttcccgct gatcgaaaaa catcagatca acgttgcctc gctggtgccg ccggcagtca gcctgtggct gcaggcgatt cacgaatggg gcagcaacgc ccagctgcaa tcgctgcagc tgctgcaggt cggcggcgcg cgcctgtcag cgacgctcgc cgcgcgcatt ccggcggaaa tcggctgtca gctgcagcag gtgttcggca tggcggaagg gctggtgaat tacacccgcc tcaacgatag cccggagcgg attatcaaca cccagggttg cccgatgtgt ccggacgacg aagtgtgggt ggcggacgcc gacggcaacc cgctgccgcg cggcgaagtt ggccgtctga tgacccgcgg tccttatacc ttccgcggct attacaacag tccgcaacat aacgctgaag cctttgatgc cgatggattt tactgctccg gtgatctgat ttcgatcgat gaagatggct atatcaccgt ccagggccgg gaaaaagatc agatcaaccg cggcggcgag aagatcgccg ctgaagagat cgaaaacctg ctgctgcgcc atgaggcggt gatccacgcc gcgctggtca gcatcgaaga caatctgctg ggcgagaaaa gctgcgctta tctggtggtg acctcgccgc tgcgcgccgt cgccgtgcgc cgcttcctgc gcgagcaggg ggtggcggaa tttaaactgc cggaccgcgt ggagtgcgtt gccgcgctgc cgctgacgcc ggtgggcaaa gtcgataaaa aacaattacg tcagtggctg gccgaaggca agctgggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat