SSGCID: Target Page

KlpnC.00404.a

3-methyl-adenine DNA glycosylase I

CENTER ID:	KlpnC.00404.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00404.a.B1.GE44230	Full length(KlpnC.00404.a)		1	193

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.4950
UniProt:	A0A0H3GX60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQRCGWVSQD PLYIEYHDTE WGVAQKEGRQ LFEMICLEGQ QAGLSWITVL KKRQNYRQAF HQFDPVRVAA MGDDDIERLL QDTGIIRHRG KIQAIIGNAR AYLAMEENGE SFTDFVWSFV DNAPQVTRAA TLAEIPTTTP ASDALSKALK KRGFKFVGST ICYSFMQACG LVNDHVTGCF CHPGGQDDPQ VGH

NT Sequence

ATGCAGCGTT GCGGATGGGT GAGTCAGGAT CCGTTATACA TCGAGTATCA CGATACCGAA TGGGGTGTGG CGCAAAAAGA GGGTCGCCAG CTGTTTGAAA TGATCTGCCT TGAGGGCCAG CAGGCCGGTT TATCGTGGAT CACGGTGTTA AAAAAACGGC AGAACTACCG CCAGGCTTTT CACCAGTTCG ATCCGGTGCG CGTGGCGGCG ATGGGTGATG ACGATATCGA GCGACTCCTG CAGGATACCG GCATCATTCG CCATCGGGGA AAAATCCAGG CCATTATCGG CAACGCCCGC GCCTATCTGG CGATGGAAGA AAATGGCGAG TCGTTCACTG ATTTTGTCTG GTCCTTCGTC GACAATGCGC CACAGGTGAC CCGCGCTGCG ACGCTGGCGG AAATTCCCAC CACGACGCCG GCCTCCGATG CGCTGTCGAA GGCATTGAAA AAACGCGGCT TTAAGTTTGT CGGCTCGACA ATCTGTTATT CCTTTATGCA GGCCTGTGGG CTGGTCAACG ACCATGTCAC CGGCTGCTTC TGTCACCCTG GAGGTCAGGA TGATCCGCAA GTGGGACAC

Details for KlpnC.00404.a.B1.GE44230

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMQ RCGWVSQDPL YIEYHDTEWG VAQKEGRQLF EMICLEGQQA GLSWITVLKK RQNYRQAFHQ FDPVRVAAMG DDDIERLLQD TGIIRHRGKI QAIIGNARAY LAMEENGESF TDFVWSFVDN APQVTRAATL AEIPTTTPAS DALSKALKKR GFKFVGSTIC YSFMQACGLV NDHVTGCFCH PGGXDDPQVG H

Validated NT Sequence

atcttactca cttagtgtcc cacttgcgga tcatcntgac ctccagggtg acagaagcag ccggtgacat ggtcgttgac cagcccacag gcctgcataa aggaataaca gattgtcgag ccgacaaact taaagccgcg ttttttcaat gccttcgaca gcgcatcgga ggccggcgtc gtggtgggaa tttccgccag cgtcgcagcg cgggtcacct gtggcgcatt gtcgacgaag gaccagacaa aatcagtgaa cgactcgcca ttttcttcca tcgccagata ggcgcgggcg ttgccgataa tggcctggat ttttccccga tggcgaatga tgccggtatc ctgcaggagt cgctcgatat cgtcatcacc catcgccgcc acgcgcaccg gatcgaactg gtgaaaagcc tggcggtagt tctgccgttt ttttaacacc gtgatccacg ataaaccggc ctgctggccc tcaaggcaga tcatttcaaa cagctggcga ccctcttttt gcgccacacc ccattcggta tcgtgatact cgatgtataa cggatcctga ctcacccatc cgcaacgctg catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc gttgcggatg ggtgagtcag gatccgttat acatcgagta tcacgatacc gaatggggtg tggcgcaaaa agagggtcgc cagctgtttg aaatgatctg ccttgagggc cagcaggccg gtttatcgtg gatcacggtg ttaaaaaaac ggcagaacta ccgccaggct tttcaccagt tcgatccggt gcgcgtggcg gcgatgggtg atgacgatat cgagcgactc ctgcaggata ccggcatcat tcgccatcgg ggaaaaatcc aggccattat cggcaacgcc cgcgcctatc tggcgatgga agaaaatggc gagtcgttca ctgattttgt ctggtccttc gtcgacaatg cgccacaggt gacccgcgct gcgacgctgg cggaaattcc caccacgacg ccggcctccg atgcgctgtc gaaggcattg aaaaaacgcg gctttaagtt tgtcggctcg acaatctgtt attcctttat gcaggcctgt gggctggtca acgaccatgt caccggctgc ttctgtcacc ctggaggtca ggatgatccg caagtgggac actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat