KlpnC.00362.a

UDP-N-acetylenolpyruvoylglucosamine reductase

CENTER ID: KlpnC.00362.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00362.a.B1.GE44227 Full length(KlpnC.00362.a) 1 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.188
UniProt: A0A0H3GPZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNHSLKPWNT FGIERFAKTI VRAETEQQLL SAWQTAAAAG EPTLILGEGS NVLFLNDYAG TVILNRIMGI EVSETPEAWR LHVGAGENWH QLVQFTLQHA MPGLENLALI PGCAGSSPIQ NIGAYGVELQ RVCEYVDCIE LETGRKQRLS AAECRFGYRD SIFKHEYQDR YAIVAIGLSL AKTWQPVLSY GDLTRLDPQT VTPQQVFDAV CHMRMTKLPD PKINGNAGSF FKNPIVSAQV AEALLAQFPH APHYPQANGS VKLAAGWLID QCELKGQRIG GAAVHRQQAL VLINEDRATS EDVVKLAHYV RQRVGAKFDV WLQPEVRFIG THGEVNAEES IA
NT Sequence
ATGAACCACT CCCTCAAACC CTGGAATACC TTTGGCATTG AACGCTTCGC CAAGACGATA GTACGCGCCG AAACTGAGCA ACAGTTGCTA AGCGCCTGGC AAACCGCTGC CGCGGCAGGC GAACCCACGC TGATCCTTGG GGAAGGCAGT AATGTCCTGT TTCTGAACGA CTATGCCGGT ACGGTGATCC TCAACCGGAT TATGGGGATC GAGGTGAGCG AAACGCCGGA AGCCTGGCGC TTGCACGTCG GCGCCGGCGA AAACTGGCAT CAACTGGTGC AGTTCACGCT ACAGCATGCG ATGCCAGGTC TGGAAAATCT GGCGTTAATC CCTGGCTGCG CAGGATCTTC GCCGATCCAG AATATTGGCG CCTACGGCGT TGAGCTACAG CGGGTTTGCG AATATGTTGA CTGCATTGAG CTGGAAACCG GGCGTAAGCA ACGTCTTTCA GCCGCGGAAT GCCGTTTTGG CTATCGCGAC AGTATCTTCA AACATGAATA TCAGGACCGC TATGCCATCG TTGCGATTGG CTTATCGTTA GCGAAAACAT GGCAGCCTGT ACTGAGTTAC GGTGATTTAA CGCGTCTCGA TCCGCAGACC GTCACGCCGC AGCAGGTATT TGATGCGGTC TGTCATATGC GAATGACTAA ATTGCCGGAT CCAAAGATTA ACGGTAATGC CGGCAGCTTT TTTAAGAACC CGATCGTCAG CGCTCAGGTT GCGGAAGCGC TGCTGGCGCA GTTCCCTCAT GCACCGCACT ATCCGCAGGC AAATGGCAGC GTCAAGCTGG CCGCTGGCTG GCTAATCGAT CAATGTGAGC TCAAAGGACA ACGCATCGGC GGCGCTGCCG TCCATCGGCA GCAGGCGCTG GTACTGATTA ATGAAGATCG AGCGACCAGC GAGGATGTGG TGAAACTTGC TCATTATGTT CGCCAGCGGG TGGGTGCGAA ATTCGATGTA TGGCTTCAGC CCGAGGTGCG CTTCATTGGC ACGCATGGTG AAGTCAACGC AGAGGAGAGC ATTGCG
Details for KlpnC.00362.a.B1.GE44227
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN HSLKPWNTFG IERFAKTIVR AETEQQLLSA WQTAAAAGEP TLILGEGSNV LFLNDYAGTV ILNRIMGIEV SETPEAWRLH VGAGENWHQL VQFTLQHAMP GLENLALIPG CAGSSPIQNI GAYGVELQRV CEYVDCIELE TGRKQRLSAA ECRFGYRDSI FKHEYQDRYA IVAIGLSLAK TWQPVLSYGD LTRLDPQTVT PQQVFDAVCH MRMTKLPDPK INGNAGSFFK NPIVSAQVAE ALLAQFPHAP HYPQANGSVK LAAGWLIDQC ELKGQRIGGA AVHRQQALVL INEDRATSED VVKLAHYVRQ RVGAKFDVWL QPEVRFIGTH GEVNAEESIA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc actccctcaa accctggaat acctttggca ttgaacgctt cgccaagacg atagtacgcg ccgaaactga gcaacagttg ctaagcgcct ggcaaaccgc tgccgcggca ggcgaaccca cgctgatcct tggggaaggc agtaatgtcc tgtttctgaa cgactatgcc ggtacggtga tcctcaaccg gattatgggg atcgaggtga gcgaaacgcc ggaagcctgg cgcttgcacg tcggcgccgg cgaaaactgg catcaactgg tgcagttcac gctacagcat gcgatgccag gtctggaaaa tctggcgtta atccctggct gcgcaggatc ttcgccgatc cagaatattg gcgcctacgg cgttgagcta cagcgggttt gcgaatatgt tgactgcatt gagctggaaa ccgggcgtaa gcaacgtctt tcagccgcgg aatgccgttt tggctatcgc gacagtatct tcaaacatga atatcaggac cgctatgcca tcgttgcgat tggcttatcg ttagcgaaaa catggcagcc tgtactgagt tacggtgatt taacgcgtct cgatccgcag accgtcacgc cgcagcaggt atttgatgcg gtctgtcata tgcgaatgac taaattgccg gatccaaaga ttaacggtaa tgccggcagc ttttttaaga acccgatcgt cagcgctcag gttgcggaag cgctgctggc gcagttccct catgcaccgc actatccgca ggcaaatggc agcgtcaagc tggccgctgg ctggctaatc gatcaatgtg agctcaaagg acaacgcatc ggcggcgctg ccgtccatcg gcagcaggcg ctggtactga ttaatgaaga tcgagcgacc agcgaggatg tggtgaaact tgctcattat gttcgccagc gggtgggtgc gaaattcgat gtatggcttc agcccgaggt gcgcttcatt ggcacgcatg gtgaagtcaa cgcagaggag agcattgcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat