KlpnC.00168.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

CENTER ID: KlpnC.00168.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00168.a.B2.GE44221 Mature protein(KlpnC.00168.a) 14 236
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4085
UniProt: A0A0H3GWT7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAATFPGVCA VVPAAGFGRR MQTECPKQYL SIGNKTILEH AVAALLADAR VQRVVIAVSP GDRRFSQLPL AQHPQITVVD GGAERADSVL AGLQALPEAQ WVLVHDAARP CLHQDDLSRL LSLCETSRVG GILAAPVRDT MKRAEPDKTA IAHTVDRNDL WHALTPQFFP RELLVDCLTR ALNEGATITD EASALEYCGF HPQLVAGRAD NIKVTRPEDL ALAEFYLTRS RHQEKA
NT Sequence
ATGGCAGCCA CTTTTCCGGG CGTTTGCGCC GTGGTGCCGG CAGCCGGTTT CGGCCGCCGC ATGCAAACGG AATGCCCGAA ACAATATCTC TCAATCGGTA ACAAAACGAT TCTCGAACAC GCGGTCGCCG CGCTGCTGGC GGATGCGCGC GTCCAGCGGG TGGTGATTGC CGTCAGCCCC GGCGATCGCC GCTTCAGCCA GCTGCCGCTG GCGCAGCATC CGCAGATTAC CGTGGTGGAT GGCGGCGCCG AGCGTGCCGA TTCGGTGCTC GCCGGCCTGC AGGCGTTGCC GGAGGCGCAG TGGGTGCTGG TGCATGACGC CGCGCGCCCC TGCCTGCATC AGGACGACCT CAGCCGCCTG CTGTCGCTCT GCGAAACCAG CCGCGTCGGC GGGATCCTTG CGGCGCCAGT GCGCGACACC ATGAAGCGCG CCGAGCCGGA CAAAACCGCT ATTGCCCATA CCGTCGACCG CAATGACCTG TGGCATGCGC TCACCCCGCA ATTTTTCCCT CGCGAGCTGC TGGTGGATTG CCTCACTCGC GCGCTAAACG AGGGAGCCAC TATCACCGAT GAAGCCTCGG CGCTGGAGTA CTGCGGCTTC CATCCGCAGC TGGTCGCCGG ACGCGCGGAT AATATTAAAG TGACGCGCCC GGAAGACCTG GCGCTGGCTG AATTTTACCT CACCCGTTCC CGACACCAGG AGAAAGCT
Details for KlpnC.00168.a.B2.GE44221
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA AGFGRRMQTE CPKQYLSIGN KTILEHAVAA LLADARVQRV VIAVSPGDRR FSQLPLAQHP QITVVDGGAE RADSVLAGLQ ALPEAQWVLV HDAARPCLHQ DDLSRLLSLC ETSRVGGILA APVRDTMKRA EPGKTAIAHT VDRNDLWHAL TPQFFPRELL VDCLTRALNE GATITDEASA LEYCGFHPQL VAGRADNIKV TRPEDLALAE FYLTRXRHQE KA
Validated NT Sequence
tcttactcac ttaagctttc tcctggtgtc ggnaacgggt gaggtaaaat tcagccagcg ccaggtcttc cgggcgcgtc actttaatat tatccgcgcg tccggcgacc agctgcggat ggaagccgca gtactcaagc gccgaggctt catcggtgat agtggctccc tcgtttagcg cgcgagtgag gcaatccacc agcagctcgc gagggaaaaa ttgcggggtg agcgcatgcc acaggtcatt gcggtcgacg gtatgggcga tagcggtttt gcccggctcg gcgcgcttca tggtgtcgcg cactggcgcc gcaaggatcc cgccgacgcg gctggtttcg cagagcgaca gcaggcggct gaggtcgtcc tgatgcaggc aggggcgcgc ggcgtcatgc accagcaccc actgcgcctc cggcaacgcc tgcaggccgg cgagtaccga atcggcgcgc tcagcgccgc catccaccac ggtaatctgc ggatgctgcg ccagcggcag ctggctgaag cggcgatcgc cggggctgac ggcaatcacc acccgctgga cgcgcgcatc cgccagcagc gcggcgaccg cgtgttcgag aatcgttttg ttaccgattg agagatattg tttcgggcat tccgtttgca tgcggcggcc gaaaccggct gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHAA GFGRRMQTEC PKQYLSIGNK TILEHAVAAL LADARVQRVV IAVSPGDRRF SQLPLAQHPQ ITVVDGGAER ADSVLAGLQA LPEAQWVLVH DAARPCLHQD DLSRLLSLCE TSRVGGILAA PVRDTMKRAE PDKTAIAHTV DRNDLWHALT PQFFPRELLV DCLTRALNEG ATITDEASAL EYCGFHPQLV AGRADNIKVT RPEDLALAEF YLTRSRHQEK A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagccg gtttcggccg ccgcatgcaa acggaatgcc cgaaacaata tctctcaatc ggtaacaaaa cgattctcga acacgcggtc gccgcgctgc tggcggatgc gcgcgtccag cgggtggtga ttgccgtcag ccccggcgat cgccgcttca gccagctgcc gctggcgcag catccgcaga ttaccgtggt ggatggcggc gccgagcgtg ccgattcggt gctcgccggc ctgcaggcgt tgccggaggc gcagtgggtg ctggtgcatg acgccgcgcg cccctgcctg catcaggacg acctcagccg cctgctgtcg ctctgcgaaa ccagccgcgt cggcgggatc cttgcggcgc cagtgcgcga caccatgaag cgcgccgagc cggacaaaac cgctattgcc cataccgtcg accgcaatga cctgtggcat gcgctcaccc cgcaattttt ccctcgcgag ctgctggtgg attgcctcac tcgcgcgcta aacgagggag ccactatcac cgatgaagcc tcggcgctgg agtactgcgg cttccatccg cagctggtcg ccggacgcgc ggataatatt aaagtgacgc gcccggaaga cctggcgctg gctgaatttt acctcacccg ttcccgacac caggagaaag cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat