SSGCID: Target Page

KlpnC.00154.a

Chorismate synthase

CENTER ID:	KlpnC.00154.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00154.a.B1.GE44220	Full length(KlpnC.00154.a)		1	361

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.3692
UniProt:	A0A0H3GWE8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGNTIGQLF RVTTFGESHG LALGCIVDGV PPGIPLTEAD LQHDLDRRRP GTSRYTTQRR EPDQVKILSG VFEGVTTGTS IGLLIENTDQ RSQDYGAIKD LFRPGHADYT YEQKYGLRDY RGGGRSSARE TAMRVAAGAI AKKYLAAKFG IVIRGCLTQM GDIPLAIKDW DQVEQNPFFC PDPDKIDALD ELMRGLKKEG DSIGAKVTVV ADGVPPGLGE PVFDRLDADI AHALMSINAV KGVEIGDGFE VVKLRGSENR DEITKAGFQS NHAGGILGGI SSGQQIVANI ALKPTSSITV PGHTINRFGE EVEMITKGRH DPCVGIRAVP IAEAMLAIVL MDHFMRQRAQ NGDVTTTIPR W

NT Sequence

ATGGCAGGAA ATACAATTGG ACAACTCTTT CGCGTCACTA CCTTCGGCGA ATCGCACGGT CTGGCGCTGG GCTGCATCGT TGACGGCGTG CCGCCGGGCA TCCCGCTGAC CGAAGCCGAC CTGCAGCACG ACCTCGATCG TCGTCGCCCG GGTACCTCGC GTTACACCAC GCAGCGTCGC GAGCCGGATC AGGTCAAAAT CCTCTCCGGC GTCTTCGAAG GCGTTACCAC CGGGACCAGT ATCGGTCTGC TGATTGAGAA CACCGATCAG CGTTCGCAGG ATTACGGCGC GATCAAAGAT CTGTTCCGTC CGGGCCACGC CGATTACACC TACGAGCAAA AGTATGGTCT GCGCGACTAT CGCGGCGGCG GTCGTTCTTC TGCGCGCGAA ACCGCGATGC GCGTAGCGGC CGGGGCGATT GCCAAAAAAT ACCTTGCCGC GAAGTTCGGC ATCGTCATTC GCGGCTGCCT GACGCAGATG GGCGATATTC CGCTGGCCAT CAAAGATTGG GATCAGGTTG AGCAGAACCC CTTCTTCTGT CCGGACCCGG ATAAAATCGA CGCCCTGGAT GAACTGATGC GCGGCCTGAA AAAAGAGGGC GACTCCATTG GCGCGAAAGT GACGGTAGTG GCTGACGGCG TACCGCCAGG CCTCGGTGAG CCGGTCTTCG ACCGCCTGGA TGCCGATATC GCCCACGCGC TGATGAGCAT CAACGCCGTG AAAGGGGTGG AAATTGGCGA CGGCTTCGAG GTGGTGAAGC TGCGCGGCAG CGAAAACCGC GATGAGATCA CCAAAGCCGG GTTCCAGAGC AACCACGCCG GCGGCATTCT TGGCGGCATC AGCAGCGGCC AGCAGATCGT TGCCAACATC GCGCTGAAGC CTACCTCCAG TATTACCGTT CCCGGTCATA CGATTAACCG CTTTGGCGAA GAAGTGGAGA TGATCACCAA AGGGCGTCAC GATCCGTGCG TCGGCATCCG CGCCGTACCG ATCGCTGAGG CGATGCTGGC GATCGTTCTG ATGGATCACT TTATGCGTCA GCGCGCGCAG AATGGCGACG TGACGACGAC AATTCCACGC TGG

Details for KlpnC.00154.a.B1.GE44220

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA GNTIGQLFRV TTFGESHGLA LGCIVDGVPP GIPLTEADLQ HDLDRRRPGT SRYTTQRREP DQVKILSGVF EGVTTGTSIG LLIENTDQRS QDYGAIKDLF RPGHADYTYE QKYGLRDYRG GGRSSARETA MRVAAGAIAK KYLAAKFGIV IRGCLTQMGD IPLAIKDWDQ VEQNPFFCPD PDKIDALDEL MRGLKKEGDS IGAKVTVVAD GVPPGLGEPV FDRLDADIAH ALMSINAVKG VEIGDGFEVV KLRGSENRDE ITKAGFQSNH AGGILGGISS GQQIVANIAL KPTSSITVPG HTINRFGEEV EMITKGRHDP CVGIRAVPIA EAMLAIVLMD HFMRQRAQNG

Validated NT Sequence

tcgccattct gcgcgcgctg acgcataaag tgatccatca gaacgatcgc cagcatcgcc tcagcgatcg gtacggcgcg gatgccgacg cacggatcgt gacgcccttt ggtgatcatc tccacttctt cgccaaagcg attaatcgta tgaccgggaa cggtaatact ggaggtaggc ttcagcgcga tgttggcaac gatctgctgg ccgctgctga taccgccaag aatgccgccg gcgtggttgc tctggaaccc ggctttagtg atctcatcgc ggttttcgct gccgcgcagc ttcaccacct cgaagccgtc gccaatttcc acccctttca cggcgttgat gctcatcagc gcgtgggcga tatcggcatc caggcggtcg aagaccggct cgccgaggcc tggcggtacg ccgtcagcca ccaccgtcac tttcgcgcca atggagtcgc cctctttttt caggccgcgc atcagttcat ccagggcgtc gattttatcc gggtccggac agaagaaggg gttctgctca acctgatccc agtctttgat ggccagcgga atatcgccca tctgcgtcag gcagccgcga atgacgatac cgaacttcgc ggcaaggtat tttttggcaa tcgccccggc cgctacgcgc atcgcggttt cgcgcgcaga agaacgaccg ccgccgcgat agtcgcgcag accatacttt tgctcgtagg tgtaatcggc gtgtcccgga cggaacagat ctttgatcgc gccgtaatcc tgcgaacgct gatcggtgtt ctcaatcagc agaccgatac tggtcccggt ggtaacgcct tcgaagacgc cggagaggat tttgacctga tccggctcgc gacgctgcgt ggtataacgc gaggtacccg ggcgacgacg atcgaggtcg tgctgcaggt cggcttcggt cagcgggatg cccggcggca cgccgtcaac gatgcagccc agcgccagac cgtgcgattc gccgaaggta gtgacgcgaa agagttgtcc aattgtattt cctgccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag gaaatacaat tggacaactc tttcgcgtca ctaccttcgg cgaatcgcac ggtctggcgc tgggctgcat cgttgacggc gtgccgccgg gcatcccgct gaccgaagcc gacctgcagc acgacctcga tcgtcgtcgc ccgggtacct cgcgttacac cacgcagcgt cgcgagccgg atcaggtcaa aatcctctcc ggcgtcttcg aaggcgttac caccgggacc agtatcggtc tgctgattga gaacaccgat cagcgttcgc aggattacgg cgcgatcaaa gatctgttcc gtccgggcca cgccgattac acctacgagc aaaagtatgg tctgcgcgac tatcgcggcg gcggtcgttc ttctgcgcgc gaaaccgcga tgcgcgtagc ggccggggcg attgccaaaa aataccttgc cgcgaagttc ggcatcgtca ttcgcggctg cctgacgcag atgggcgata ttccgctggc catcaaagat tgggatcagg ttgagcagaa ccccttcttc tgtccggacc cggataaaat cgacgccctg gatgaactga tgcgcggcct gaaaaaagag ggcgactcca ttggcgcgaa agtgacggta gtggctgacg gcgtaccgcc aggcctcggt gagccggtct tcgaccgcct ggatgccgat atcgcccacg cgctgatgag catcaacgcc gtgaaagggg tggaaattgg cgacggcttc gaggtggtga agctgcgcgg cagcgaaaac cgcgatgaga tcaccaaagc cgggttccag agcaaccacg ccggcggcat tcttggcggc atcagcagcg gccagcagat cgttgccaac atcgcgctga agcctacctc cagtattacc gttcccggtc atacgattaa ccgctttggc gaagaagtgg agatgatcac caaagggcgt cacgatccgt gcgtcggcat ccgcgccgta ccgatcgctg aggcgatgct ggcgatcgtt ctgatggatc actttatgcg tcagcgcgcg cagaatggcg acgtgacgac gacaattcca cgctggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t