KlpnC.00150.a
Bifunctional protein GlmU
| CENTER ID: | KlpnC.00150.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.00150.a.B1.PW38985 | Full length(KlpnC.00150.a) | 1 | 456 |
Structures
8CU9
DEPOSITED: 5/16/2022
DETERMINATION: XRay
CLONE: KlpnC.00150.a.B1.GE44219
PROTEIN: KlpnC.00150.a.B1.PW38985
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.5182 |
| UniProt: | A0A0H3GVB1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNSAMSVVI LAAGKGTRMY SDLPKVLHTL AGKPMVQHVI DAANDLGACA VHLVYGHGGD LLRQTLHEDN LNWVLQAEQL GTGHAMQQAA PFFNDDEDIL MLYGDVPLIS VETLQRLRAA KPQGGIGLLT VKLDDPTGYG RITRENGQVT GIVEHKDASE AQRQIQEINT GILIAGGADL KRWLAKLTNN NAQGEYYITD IIAMAHQEGH QIVAVHPQRL SEVEGVNNRL QLARLERVYQ AEQAEKLLLA GVMLRDPARF DLRGTLQHGR DVEIDTNVIL EGNVVLGDRV KIGAGCVIKN STIGDDCEIS PYSVVEDAQL QAACTIGPFA RLRPGAELLA GAHVGNFVEM KKARLGKGSK AGHLTYLGDA EIGDNVNIGA GTITCNYDGA NKHKTIIGDD VFVGSDTQLV APVTVGNGVT IAAGTTVTRN IADNELVLSR VPQVHKQGWQ RPVKKK
NT Sequence
ATGTCAAATA GTGCGATGAG CGTGGTTATC CTTGCTGCAG GCAAAGGGAC CCGCATGTAT TCCGATCTTC CTAAGGTGCT GCATACCCTG GCCGGGAAGC CAATGGTGCA GCATGTCATT GATGCCGCTA ACGATTTAGG CGCATGTGCG GTACACCTGG TATACGGCCA CGGCGGCGAT CTGCTGCGTC AGACTCTGCA TGAAGACAAC CTGAACTGGG TGCTGCAGGC CGAGCAGCTC GGAACCGGGC ACGCTATGCA GCAGGCGGCG CCATTCTTCA ATGATGATGA AGACATCCTG ATGCTCTATG GCGACGTGCC GCTGATCTCC GTTGAGACGC TACAGCGCCT GCGCGCCGCG AAACCACAGG GTGGGATTGG TCTGTTAACG GTGAAGCTGG ATGACCCAAC CGGCTATGGC CGCATTACCC GTGAAAATGG CCAGGTGACG GGCATCGTTG AGCACAAAGA TGCCAGCGAG GCCCAGCGCC AGATCCAGGA AATCAACACC GGGATCCTGA TCGCCGGCGG AGCGGATCTA AAGCGCTGGC TGGCGAAACT CACCAATAAC AACGCTCAGG GTGAATACTA CATCACCGAT ATCATCGCCA TGGCGCACCA GGAAGGACAC CAGATCGTCG CAGTGCATCC GCAGCGCCTG AGCGAAGTCG AAGGCGTCAA CAACCGCCTG CAGCTTGCGC GCCTGGAGCG CGTCTACCAG GCGGAGCAGG CGGAAAAGCT GCTGCTGGCG GGCGTGATGC TGCGCGATCC GGCGCGTTTC GATCTGCGCG GTACGCTGCA GCACGGGCGC GATGTAGAGA TCGATACTAA CGTTATTCTG GAAGGCAACG TGGTGCTGGG CGATCGAGTG AAAATCGGCG CCGGCTGCGT GATCAAAAAC AGCACCATCG GCGATGACTG CGAAATTAGC CCCTACAGCG TGGTGGAAGA TGCCCAGCTG CAGGCGGCCT GCACCATCGG TCCATTTGCC CGTCTGCGCC CGGGCGCCGA ACTGCTGGCA GGCGCCCACG TTGGTAACTT CGTGGAAATG AAAAAAGCGC GTCTCGGGAA AGGCTCCAAG GCGGGTCATC TGACCTATCT GGGCGATGCG GAGATTGGCG ATAACGTCAA TATCGGCGCG GGCACCATCA CCTGCAACTA CGATGGTGCG AACAAGCATA AAACGATTAT TGGCGATGAT GTCTTTGTCG GCTCGGATAC CCAGCTGGTG GCCCCGGTCA CGGTAGGCAA CGGCGTCACG ATCGCCGCCG GCACCACCGT CACGCGCAAT ATCGCCGATA ACGAACTGGT GTTGAGCCGC GTACCGCAGG TCCACAAACA GGGCTGGCAG CGTCCGGTGA AGAAGAAA
Details for KlpnC.00150.a.B1.PW38985
| PURIFICATION DATe: | 5/7/2021 |
| CONCENTRATION: | 17mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NSAMSVVILA AGKGTRMYSD LPKVLHTLAG KPMVQHVIDA ANDLGACAVH LVYGHGGDLL RQTLHEDNLN WVLQAEQLGT GHAMQQAAPF FNDDEDILML YGDVPLISVE TLQRLRAAKP QGGIGLLTVK LDDPTGYGRI TRENGQVTGI VEHKDASEAQ RQIQEINTGI LIAGGADLKR WLAKLTNNNA QGEYYITDII AMAHXEGHQI VAVHPQRLSE VEGVNNRLQL ARLERVYQAE QAEKLLLAGV MLRDPARFDL RGTLQHGRDV EIDTNVILEG NVVLGDRVKI GAGCVIKNST IGDDCEISPY SVVEDAQLQA ACTIGPFARL RPGAELLEGA HVGNFVEMKK ARLGKGSKAG HLTYLGDAEI GDNVNIGAGT ITCNYDGANK HKTIIGDDVF VGSDTQLVAP VTVGNGVTIA AGTTVTRNIA DNELVLSRVP QVHKQGWQRP VKKK
Validated NT Sequence
atggctcacc accaccacca ccatatgtca aatagtgcga tgagcgtggt tatccttgct gcaggcaaag ggacccgcat gtattccgat cttcctaagg tgctgcatac cctggccggg aagccaatgg tgcagcatgt cattgatgcc gctaacgatt taggcgcatg tgcggtacac ctggtatacg gccacggggg cgatctgctg cgtcagactc tgcatgaaga caacctgaac tgggtgctgc aggctgagca gctcggaacc gggcacgcta tgcagcaggc ggcgccattc ttcaatgatg atgaagacat cctgatgctc tatggcgacg tgccgctgat ctccgttgag acgctacagc gcctgcgcgc cgcgaaacca cagggtggga ttggtctgtt aacggtgaag ctggatgacc caaccggcta tggccgcatt acccgtgaaa atggccaggt gacgggcatc gttgagcaca aagatgccag cgaggcccag cgccagatcc aggaaatcaa caccgggatc ctgatcgccg gcggagcgga tctaaagcgc tggctggcga aactcaccaa taacaacgct cagggcgaat actacatcac cgatatcatc gccatggcgc accangaagg acaccagatc gtcgcagtgc atccgcagcg cctgagcgaa gtcgaaggcg tcaacaaccg nctgcagctt gcgcgcctgg agcgcgtcta ccaggcggag caggcggaaa agctgctgct ggcgggcgtg atgctgcgcg atccggcgcg tttcgatctg cgcggtacgc tgcagcacgg gcgcgatgta gagatcgata ctaacgttat tctggaaggc aacgtggtgc tgggcgatcg agtgaaaatc ggcgccggct gcgtgatcaa aaacagcacc atcggcgatg actgcgaaat tagcccctac agcgtggtgg aagatgccca gctgcaggcg gcctgcacca tcggtccatt tgcccgtctg cgcccgggcg ccgaactgct ggaaggcgcc cacgttggta acttcgtgga aatgaaaaaa gcgcgtctcg ggaaaggctc caaggcgggt catctgacct atctgggcga tgcggagatt ggcgataacg tcaatatcgg cgcgggcacc atcacctgca actacgatgg tgcgaacaag cataaaacga ttattggcga tgatgtcttt gtcggctcgg atacccagct ggtggcgcca gtcacggtag gcaatggcgt tactatcgcc gccggcacca ccgttacgcg caatatcgcc gataatgagc tggtgttgag ccgcgtaccg caggtccaca aacagggctg gcagcgtccg gtgaagaaga aataa
Expressed Protein Sequence
MAHHHHHHMS NSAMSVVILA AGKGTRMYSD LPKVLHTLAG KPMVQHVIDA ANDLGACAVH LVYGHGGDLL RQTLHEDNLN WVLQAEQLGT GHAMQQAAPF FNDDEDILML YGDVPLISVE TLQRLRAAKP QGGIGLLTVK LDDPTGYGRI TRENGQVTGI VEHKDASEAQ RQIQEINTGI LIAGGADLKR WLAKLTNNNA QGEYYITDII AMAHQEGHQI VAVHPQRLSE VEGVNNRLQL ARLERVYQAE QAEKLLLAGV MLRDPARFDL RGTLQHGRDV EIDTNVILEG NVVLGDRVKI GAGCVIKNST IGDDCEISPY SVVEDAQLQA ACTIGPFARL RPGAELLAGA HVGNFVEMKK ARLGKGSKAG HLTYLGDAEI GDNVNIGAGT ITCNYDGANK HKTIIGDDVF VGSDTQLVAP VTVGNGVTIA AGTTVTRNIA DNELVLSRVP QVHKQGWQRP VKKK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAA ATAGTGCGAT GAGCGTGGTT ATCCTTGCTG CAGGCAAAGG GACCCGCATG TATTCCGATC TTCCTAAGGT GCTGCATACC CTGGCCGGGA AGCCAATGGT GCAGCATGTC ATTGATGCCG CTAACGATTT AGGCGCATGT GCGGTACACC TGGTATACGG CCACGGCGGC GATCTGCTGC GTCAGACTCT GCATGAAGAC AACCTGAACT GGGTGCTGCA GGCCGAGCAG CTCGGAACCG GGCACGCTAT GCAGCAGGCG GCGCCATTCT TCAATGATGA TGAAGACATC CTGATGCTCT ATGGCGACGT GCCGCTGATC TCCGTTGAGA CGCTACAGCG CCTGCGCGCC GCGAAACCAC AGGGTGGGAT TGGTCTGTTA ACGGTGAAGC TGGATGACCC AACCGGCTAT GGCCGCATTA CCCGTGAAAA TGGCCAGGTG ACGGGCATCG TTGAGCACAA AGATGCCAGC GAGGCCCAGC GCCAGATCCA GGAAATCAAC ACCGGGATCC TGATCGCCGG CGGAGCGGAT CTAAAGCGCT GGCTGGCGAA ACTCACCAAT AACAACGCTC AGGGTGAATA CTACATCACC GATATCATCG CCATGGCGCA CCAGGAAGGA CACCAGATCG TCGCAGTGCA TCCGCAGCGC CTGAGCGAAG TCGAAGGCGT CAACAACCGC CTGCAGCTTG CGCGCCTGGA GCGCGTCTAC CAGGCGGAGC AGGCGGAAAA GCTGCTGCTG GCGGGCGTGA TGCTGCGCGA TCCGGCGCGT TTCGATCTGC GCGGTACGCT GCAGCACGGG CGCGATGTAG AGATCGATAC TAACGTTATT CTGGAAGGCA ACGTGGTGCT GGGCGATCGA GTGAAAATCG GCGCCGGCTG CGTGATCAAA AACAGCACCA TCGGCGATGA CTGCGAAATT AGCCCCTACA GCGTGGTGGA AGATGCCCAG CTGCAGGCGG CCTGCACCAT CGGTCCATTT GCCCGTCTGC GCCCGGGCGC CGAACTGCTG GCAGGCGCCC ACGTTGGTAA CTTCGTGGAA ATGAAAAAAG CGCGTCTCGG GAAAGGCTCC AAGGCGGGTC ATCTGACCTA TCTGGGCGAT GCGGAGATTG GCGATAACGT CAATATCGGC GCGGGCACCA TCACCTGCAA CTACGATGGT GCGAACAAGC ATAAAACGAT TATTGGCGAT GATGTCTTTG TCGGCTCGGA TACCCAGCTG GTGGCCCCGG TCACGGTAGG CAACGGCGTC ACGATCGCCG CCGGCACCAC CGTCACGCGC AATATCGCCG ATAACGAACT GGTGTTGAGC CGCGTACCGC AGGTCCACAA ACAGGGCTGG CAGCGTCCGG TGAAGAAGAA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT