SSGCID: Target Page

KlpnC.00149.a

Ubiquinone biosynthesis O-methyltransferase

CENTER ID:	KlpnC.00149.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00149.a.B1.GE44218	Full length(KlpnC.00149.a)		1	242

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00149.a.B1.PW38984	Full length(KlpnC.00149.a)		1	242

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.3612
UniProt:	A0A0H3GR24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAEKTSVAP NVDHAEIAKF EAVASRWWDL EGEFKPLHRI NPLRLGYIAE RSGGLFGKKV LDVGCGGGIL AESMAREGAT VTGLDMGAEP LQVARLHALE SGIQVDYVQE TVEEHAAKHP QQYDVVTCME MLEHVPDPQS VVHACARLVK PGGQVFFSTI NRNGKAWLMA VVGAEYVMKM VPKGTHDVKK FIKPAELLGW VDQTTLKEQH IIGLHYNPLT NTFKLAPGVD VNYMLHTTAK QD

NT Sequence

ATGAATGCCG AAAAAACGTC GGTAGCCCCT AACGTCGATC ATGCTGAAAT CGCCAAATTC GAAGCCGTCG CCTCGCGCTG GTGGGATCTG GAGGGCGAGT TCAAACCGCT CCACCGCATC AACCCGCTGC GGCTGGGCTA TATTGCCGAA CGCTCCGGCG GCCTGTTCGG TAAAAAAGTC CTCGATGTCG GCTGCGGCGG CGGCATCCTC GCCGAGAGCA TGGCGCGCGA AGGCGCGACG GTCACCGGGC TGGACATGGG GGCCGAACCG CTGCAGGTCG CCAGGCTGCA CGCCCTGGAG AGCGGTATTC AGGTAGACTA TGTTCAGGAA ACAGTTGAGG AACATGCCGC GAAGCACCCG CAGCAGTATG ACGTCGTGAC CTGCATGGAG ATGCTGGAGC ACGTCCCCGA CCCGCAGTCG GTGGTGCACG CCTGCGCGCG GTTAGTGAAG CCCGGCGGCC AGGTCTTCTT CTCCACCATC AACCGCAACG GCAAGGCCTG GCTGATGGCG GTAGTCGGGG CGGAATATGT CATGAAGATG GTGCCCAAAG GCACTCATGA TGTGAAGAAG TTTATCAAGC CGGCCGAGCT GCTGGGCTGG GTAGATCAGA CTACTCTGAA AGAGCAGCAT ATCATCGGTC TGCATTACAA TCCGCTGACC AATACCTTCA AGTTAGCGCC GGGGGTTGAT GTTAACTATA TGTTGCATAC AACCGCCAAA CAGGAC

Details for KlpnC.00149.a.B1.GE44218

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	48

Validated AA Sequence

MAHHHHHHMN AEKTSVAPNV DHAEIAKFEA VASRWWDLEG EFKPLHRINP LRLGYIAERS GGLFGKKVLD VGCGGGILAE SMAREGATVT GLDMGAEPLQ VARLHALESG IQVDYVQETV

Validated NT Sequence

aannnannan nnacttcact atagggagac caccnggttt ccctctagaa ntaattttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat gaatgccgaa aaaacgtcgg tagcccctaa cgtcgatcat gctgaaatcg ccaaattcga agccgtcgcc tcgcgctggt gggatctgga gggcgagttc aaaccgctcc accgcatcaa cccgctgcgg ctgggctata ttgccgaacg ctccggcggc ctgttcggta aaaaagtcct cgatgtcggc tgcggcggcg gcatcctcgc cgagagcatg gcgcgcgaag gcgcgacggt caccgggctg gacatggggg ccgaaccgct gcaggtcgcc aggctgcacg ccctggagag cggtattcag gtagactatg ttcaggaaac agtttgagga acatgccgcg aagcacccgc agcagtatga cgtcgtgacc tgcatggaga tgctggagca cgtccccgac ccgcagtcgg tggtgcacgc ctgcgcgcgg ttagtgaagc ccggnggcca ggtcttcttc tccaccatca accgcaacgg caaggcctgg ctgatggcgg tagtcggggc ggaatatgtc atgaagatgg tgcccaaagg cactcatgat gtgaagaagn ttatcnaagc cggccganct gctgggctgg gtagatcaga ctactctgaa agagcagcat atcatcnggt ctgcattaca atccgctgac cnataccttc nagttagcgc cgggggttga tgttaactat atgtttgcat acaaccgcca ancaggactn aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaanctgt agttggctgc tgccancgct gagcannacn tagcataacc cntngggcnt ctaacnggtc ttgagggggt ttttgctgaa aggaggaact atatccggan attcacnnga cnggngnngg ncnnnntgat cncg

Expected Protein Sequence

MAHHHHHHMN AEKTSVAPNV DHAEIAKFEA VASRWWDLEG EFKPLHRINP LRLGYIAERS GGLFGKKVLD VGCGGGILAE SMAREGATVT GLDMGAEPLQ VARLHALESG IQVDYVQETV EEHAAKHPQQ YDVVTCMEML EHVPDPQSVV HACARLVKPG GQVFFSTINR NGKAWLMAVV GAEYVMKMVP KGTHDVKKFI KPAELLGWVD QTTLKEQHII GLHYNPLTNT FKLAPGVDVN YMLHTTAKQD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg ccgaaaaaac gtcggtagcc cctaacgtcg atcatgctga aatcgccaaa ttcgaagccg tcgcctcgcg ctggtgggat ctggagggcg agttcaaacc gctccaccgc atcaacccgc tgcggctggg ctatattgcc gaacgctccg gcggcctgtt cggtaaaaaa gtcctcgatg tcggctgcgg cggcggcatc ctcgccgaga gcatggcgcg cgaaggcgcg acggtcaccg ggctggacat gggggccgaa ccgctgcagg tcgccaggct gcacgccctg gagagcggta ttcaggtaga ctatgttcag gaaacagttg aggaacatgc cgcgaagcac ccgcagcagt atgacgtcgt gacctgcatg gagatgctgg agcacgtccc cgacccgcag tcggtggtgc acgcctgcgc gcggttagtg aagcccggcg gccaggtctt cttctccacc atcaaccgca acggcaaggc ctggctgatg gcggtagtcg gggcggaata tgtcatgaag atggtgccca aaggcactca tgatgtgaag aagtttatca agccggccga gctgctgggc tgggtagatc agactactct gaaagagcag catatcatcg gtctgcatta caatccgctg accaatacct tcaagttagc gccgggggtt gatgttaact atatgttgca tacaaccgcc aaacaggact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for KlpnC.00149.a.B1.PW38984

PURIFICATION DATe:	5/7/2021
CONCENTRATION:	5mg/ml
OBSERVED MW:	28kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	48

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMN AEKTSVAPNV DHAEIAKFEA VASRWWDLEG EFKPLHRINP LRLGYIAERS GGLFGKKVLD VGCGGGILAE SMAREGATVT GLDMGAEPLQ VARLHALESG IQVDYVQETV

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG CCGAAAAAAC GTCGGTAGCC CCTAACGTCG ATCATGCTGA AATCGCCAAA TTCGAAGCCG TCGCCTCGCG CTGGTGGGAT CTGGAGGGCG AGTTCAAACC GCTCCACCGC ATCAACCCGC TGCGGCTGGG CTATATTGCC GAACGCTCCG GCGGCCTGTT CGGTAAAAAA GTCCTCGATG TCGGCTGCGG CGGCGGCATC CTCGCCGAGA GCATGGCGCG CGAAGGCGCG ACGGTCACCG GGCTGGACAT GGGGGCCGAA CCGCTGCAGG TCGCCAGGCT GCACGCCCTG GAGAGCGGTA TTCAGGTAGA CTATGTTCAG GAAACAGTTG AGGAACATGC CGCGAAGCAC CCGCAGCAGT ATGACGTCGT GACCTGCATG GAGATGCTGG AGCACGTCCC CGACCCGCAG TCGGTGGTGC ACGCCTGCGC GCGGTTAGTG AAGCCCGGCG GCCAGGTCTT CTTCTCCACC ATCAACCGCA ACGGCAAGGC CTGGCTGATG GCGGTAGTCG GGGCGGAATA TGTCATGAAG ATGGTGCCCA AAGGCACTCA TGATGTGAAG AAGTTTATCA AGCCGGCCGA GCTGCTGGGC TGGGTAGATC AGACTACTCT GAAAGAGCAG CATATCATCG GTCTGCATTA CAATCCGCTG ACCAATACCT TCAAGTTAGC GCCGGGGGTT GATGTTAACT ATATGTTGCA TACAACCGCC AAACAGGACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT