KlpnC.00137.a
D-alanine--D-alanine ligase
| CENTER ID: | KlpnC.00137.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.00137.a.B1.GE44214 | Full length(KlpnC.00137.a) | 1 | 376 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7U56
DEPOSITED: 3/1/2022
DETERMINATION: XRay
CLONE: KlpnC.00137.a.B1.GE44214
PROTEIN: KlpnC.00137.a.B1.PW38983
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.1029 |
| UniProt: | A0A0H3GSI5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPFDVIWMEV EMAKMRVGIV FGGKSAEHEV SLQSAKNIVE AIDKSRFDVV LLGIDKQGLW HINDAGNYLL NAQDPARIAL RPSTVTLAQI PGREAQQLIN AESGQPLAAI DVIFPIVHGT LGEDGSLQGM LRMANLPFVG SDVLGSAACM DKDVTKRLLR DAGLAVAPFI TLTRANRAQF SFADVEAKLG LPLFVKPANQ GSSVGVSKVK NEEQYHQAVA LAFEFDHKVV VEQGIKGREI ECAVLGNDHP QASTCGEIVL NSEFYAYDTK YIDDQGAQVV VPAAIAPEIN DKIRAIAVQA YQTLGCSGMA RVDVFLTADN EVVINEINTL PGFTNISMYP KLWQASGLDY TSLITRLIEL ALERHAADRA LKTSMN
NT Sequence
ATGCCCTTTG ATGTTATCTG GATGGAAGTT GAAATGGCAA AGATGAGGGT TGGGATCGTT TTTGGCGGCA AGTCGGCGGA GCATGAAGTG TCGCTGCAGT CAGCGAAGAA TATCGTGGAA GCGATTGATA AATCGCGCTT CGATGTGGTT CTGTTGGGTA TTGATAAACA GGGGCTGTGG CATATCAACG ACGCCGGCAA CTACCTGCTC AACGCACAGG ATCCTGCCCG CATTGCCCTG CGCCCTTCCA CGGTGACTCT GGCGCAGATC CCGGGCCGCG AAGCGCAGCA GCTGATTAAC GCCGAAAGCG GCCAACCGCT GGCGGCCATC GATGTGATTT TCCCGATCGT CCACGGCACG CTGGGTGAAG ATGGCTCCCT GCAGGGGATG CTGCGCATGG CGAATCTGCC GTTCGTCGGC TCCGATGTGC TGGGCTCTGC GGCCTGTATG GACAAAGACG TGACCAAACG TCTGCTGCGC GACGCCGGGC TCGCCGTCGC CCCGTTTATC ACACTCACCC GCGCCAACCG CGCGCAGTTC AGCTTTGCCG ATGTCGAAGC GAAGCTTGGC CTGCCGCTGT TCGTTAAGCC GGCTAATCAG GGCTCGTCGG TTGGCGTCAG CAAAGTTAAA AACGAAGAGC AGTACCATCA GGCGGTGGCC CTGGCCTTCG AATTTGACCA TAAAGTGGTG GTGGAGCAAG GCATTAAAGG GCGGGAAATT GAGTGTGCGG TGCTGGGTAA CGACCACCCG CAGGCCAGCA CCTGCGGTGA AATCGTCCTC AACAGCGAGT TTTACGCCTA CGACACCAAG TATATCGACG ATCAGGGCGC ACAGGTGGTA GTCCCGGCCG CTATCGCCCC TGAGATCAAC GACAAAATTC GCGCTATCGC GGTGCAGGCC TACCAGACCC TGGGCTGCAG CGGCATGGCT CGTGTCGACG TGTTCCTCAC CGCCGACAAC GAGGTGGTGA TCAACGAGAT CAACACCCTG CCGGGCTTTA CCAACATCAG CATGTATCCG AAGCTGTGGC AGGCCAGCGG TCTCGACTAC ACCAGCCTGA TCACCCGCCT GATTGAGCTG GCGTTGGAGC GCCATGCCGC GGACCGGGCG CTGAAAACCT CCATGAAC
Details for KlpnC.00137.a.B1.GE44214
| HARVESTED ON: | 4/1/2021 |
| SEQUENCED ON: | 4/13/2021 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 25 |
| PERCENT COVERAGE: | 78 |
Validated AA Sequence
MALQRQLNQA GDQAGVVETA GLPQLRIHAD VGKARQGVDL VDHHLVVGGE EHVDTSHAAA AQGLVGLHRD SANFVVDLRG DSGRDYHLRA LIVDILGVVG VKLAVEDDFT AGAGLRVVVT QHRTLNFPPF NALLHHHFMV KFEGQGHRLM VLLFVFNFAD ANRRALISRL NEQRQAKLRF DIGKAELRAV GAGECDKRGD GEPGVAQQTF GHVFVHTGRR AQHIGADERQ IRHAQHPLQG AIFTXACRGR SGKSHRWPPA GXPLSALISC CASRPGXCAR VTVXXAXMRA XSCALSXSCR XX
Validated NT Sequence
tttcgggctt tgttagcagc cggatcctat cttactcact tagttcatgg aggttttcag nnccnggtcc gcggcatggc gctccaacgc cagctcaatc aggcgggtga tcaggctggt gtagtcgaga ccgctggcct gccacagctt cggatacatg ctgatgttgg taaagcccgg cagggtgttg atctcgttga tcaccacctc gttgtcggcg gtgaggaaca cgtcgacacg agccatgccg ctgcagccca gggtctggta ggcctgcacc gcgatagcgc gaattttgtc gttgatctca ggggcgatag cggccgggac taccacctgc gcgccctgat cgtcgatata cttggtgtcg taggcgtaaa actcgctgtt gaggacgatt tcaccgcagg tgctggcctg cgggtggtcg ttacccagca ccgcacactc aatttcccgc cctttaatgc cttgctccac caccacttta tggtcaaatt cgaaggccag ggccaccgcc tgatggtact gctcttcgtt tttaactttg ctgacgccaa ccgacgagcc ctgattagcc ggcttaacga acagcggcag gccaagcttc gcttcgacat cggcaaagct gaactgcgcg cggttggcgc gggtgagtgt gataaacggg gcgacggcga gcccggcgtc gcgcagcaga cgtttggtca cgtctttgtc catacaggcc gcagagccca gcacatcgga gccgacgaac ggcagattcg ccatgcgcag catcccctgc agggagccat cttcaccnca gcgtgccgtg gncgatcggg aaaatcacat cgatggccgc cagcgggtng gccgctttcn gcgttaatca gctgctgcgc ttcgcggccc gggntctgcg ccagagtcac cgtggannnc gcgnnaatgc gggcnngatc ctgtgcgttg agcnnnagtt gccggngtcn ttganatgcc ncanncctgn ttnntcaata cccnnnnnan nncatcnaag cgcgaattna tcantc
Expected Protein Sequence
MAHHHHHHMP FDVIWMEVEM AKMRVGIVFG GKSAEHEVSL QSAKNIVEAI DKSRFDVVLL GIDKQGLWHI NDAGNYLLNA QDPARIALRP STVTLAQIPG REAQQLINAE SGQPLAAIDV IFPIVHGTLG EDGSLQGMLR MANLPFVGSD VLGSAACMDK DVTKRLLRDA GLAVAPFITL TRANRAQFSF ADVEAKLGLP LFVKPANQGS SVGVSKVKNE EQYHQAVALA FEFDHKVVVE QGIKGREIEC AVLGNDHPQA STCGEIVLNS EFYAYDTKYI DDQGAQVVVP AAIAPEINDK IRAIAVQAYQ TLGCSGMARV DVFLTADNEV VINEINTLPG FTNISMYPKL WQASGLDYTS LITRLIELAL ERHAADRALK TSMN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccct ttgatgttat ctggatggaa gttgaaatgg caaagatgag ggttgggatc gtttttggcg gcaagtcggc ggagcatgaa gtgtcgctgc agtcagcgaa gaatatcgtg gaagcgattg ataaatcgcg cttcgatgtg gttctgttgg gtattgataa acaggggctg tggcatatca acgacgccgg caactacctg ctcaacgcac aggatcctgc ccgcattgcc ctgcgccctt ccacggtgac tctggcgcag atcccgggcc gcgaagcgca gcagctgatt aacgccgaaa gcggccaacc gctggcggcc atcgatgtga ttttcccgat cgtccacggc acgctgggtg aagatggctc cctgcagggg atgctgcgca tggcgaatct gccgttcgtc ggctccgatg tgctgggctc tgcggcctgt atggacaaag acgtgaccaa acgtctgctg cgcgacgccg ggctcgccgt cgccccgttt atcacactca cccgcgccaa ccgcgcgcag ttcagctttg ccgatgtcga agcgaagctt ggcctgccgc tgttcgttaa gccggctaat cagggctcgt cggttggcgt cagcaaagtt aaaaacgaag agcagtacca tcaggcggtg gccctggcct tcgaatttga ccataaagtg gtggtggagc aaggcattaa agggcgggaa attgagtgtg cggtgctggg taacgaccac ccgcaggcca gcacctgcgg tgaaatcgtc ctcaacagcg agttttacgc ctacgacacc aagtatatcg acgatcaggg cgcacaggtg gtagtcccgg ccgctatcgc ccctgagatc aacgacaaaa ttcgcgctat cgcggtgcag gcctaccaga ccctgggctg cagcggcatg gctcgtgtcg acgtgttcct caccgccgac aacgaggtgg tgatcaacga gatcaacacc ctgccgggct ttaccaacat cagcatgtat ccgaagctgt ggcaggccag cggtctcgac tacaccagcc tgatcacccg cctgattgag ctggcgttgg agcgccatgc cgcggaccgg gcgctgaaaa cctccatgaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat