KlpnC.00118.a

Alpha-D-glucosyl-1-phosphate uridylyltransferase

CENTER ID: KlpnC.00118.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00118.a.B2.GE44212 Mature protein(KlpnC.00118.a) 25 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3481
UniProt: A0A0H3GV99

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNMANLKAVI PVAGLGMHML PATKAIPKEM LPIVDKPMIQ YIVDEIVAAG IKEIVLVTHS SKNAVENHFD TSYELEALLE QRVKRQLLAE VQAICPPGVT IMNVRQAQPL GLGHSILCAR PVVGDNPFVV VLPDIILDGG TADPLRYNLA AMIARFNETG RSQVLAKRMP GDLSEYSVIQ TKEPMVAEGQ VARIVEFIEK PDEPQTLDSD LMAVGRYVLS ADIWAELERT EPGAWGRIQL TDAIAELAKK PSVDAMLMTG ESYDCGKKMG YMQAFVTYGM RNLKEGTKFR ESIKKNAGLT AGVKMSGRAE PFGTPLITLC RNYS
NT Sequence
ATGAATATGG CGAATTTGAA AGCGGTTATT CCGGTCGCAG GACTAGGCAT GCATATGCTG CCGGCCACAA AGGCAATTCC AAAGGAGATG CTGCCGATCG TTGATAAGCC AATGATTCAG TACATCGTTG ACGAAATCGT CGCCGCCGGG ATCAAAGAGA TTGTTCTGGT GACGCACTCC TCGAAGAACG CGGTGGAAAA CCACTTCGAC ACATCCTACG AGCTGGAAGC CCTGCTGGAA CAGCGCGTGA AGCGCCAGCT GCTGGCGGAA GTGCAGGCCA TTTGCCCGCC GGGCGTGACC ATCATGAACG TGCGTCAGGC GCAGCCGCTG GGCCTGGGCC ACTCCATCCT CTGCGCCCGC CCGGTAGTCG GCGACAACCC GTTTGTGGTG GTCCTGCCGG ATATCATCCT TGACGGCGGC ACCGCTGACC CGCTGCGCTA TAACCTCGCC GCTATGATCG CCCGCTTTAA CGAGACCGGC CGCAGCCAGG TGCTGGCGAA ACGCATGCCG GGCGATCTTT CCGAATACTC CGTCATCCAG ACCAAAGAGC CGATGGTGGC TGAAGGCCAG GTGGCGCGTA TCGTCGAGTT TATTGAAAAA CCGGACGAGC CGCAGACTCT GGACTCCGAT CTGATGGCGG TGGGGCGCTA CGTGCTGTCG GCGGATATCT GGGCCGAACT GGAACGTACC GAGCCGGGCG CATGGGGCCG TATCCAGCTG ACTGACGCCA TTGCCGAACT GGCGAAGAAG CCGTCCGTCG ACGCGATGCT GATGACCGGC GAAAGCTACG ACTGCGGGAA GAAAATGGGC TACATGCAGG CCTTCGTCAC CTACGGCATG CGTAACCTGA AAGAAGGCAC CAAATTCCGC GAAAGTATTA AAAAAAATGC TGGCCTGACA GCTGGCGTAA AGATGAGCGG CAGGGCAGAG CCTTTCGGCA CGCCGCTCAT AACGTTATGC AGAAATTATT CA
Details for KlpnC.00118.a.B2.GE44212
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMA IPKEMLPIVD KPMIQYIVDE IVAAGIKEIV LVTHSSKNAV ENHFDTSYEL EALLEQRVKR QLLAEVQAIC PPGVTIMNVR QAQPLGLGHS ILCARPVVGD NPFVVVLPDI ILDGGTADPL RYNLAAMIAR FNETGRSQVL AKRMPGDLSE YSVIQTKEPM VAEGQVARIV EFIEKPDEPQ TLDSDLMAVG RYVLSADIWA ELERTEPGAW GRIQLTDAIA ELAKKQSVDA MLMTGESYDC GKKMGYMQAF VTYGMRNLKE GAKFRESIKK LLA
Validated NT Sequence
atcttactca cttatgaata atttctgcat aacgtnatga gcgacaggac gaatgttcac gccctgcccc cccgtactac gctagcaatc aggccagcag ttttttaata ctttcgcgga atttggcgcc ttctttcagg ttacgcatcc cgtaggtgac gaaggcctgc atgtagccca ttttcttccc gcagtcgtag ctttcgccgg tcatcagcat cgcgtcgacg gattgcttct tcgccagttc ggcaatggcg tcggtcagtt ggatacggcc ccatgcgccc ggctcggtac gttccagttc ggcccagata tccgccgaca gcacgtagcg ccccaccgcc atcagatcgg agtccagggt ctgcggttcg tccggttttt caataaactc gacgatacgc gccacctggc cttcagccac catcggctct ttggtctgga tgacggagta ttcggagaga tcgcccggca tacgtttcgc cagcacctgg ctgcggccgg tctcgttaaa gcgggcgatc atagcggcga ggttatagcg cagcgggtca gcggtgccgc cgtcaaggat gatatccggc aggaccacca caaacgggtt gtcgccgact accgggcggg cgcagaggat ggagtggccc aggcccagcg gctgcgcctg acgcacgttc atgatggtca cgcccggcgg gcaaatggcc tgcacttccg ccagcagctg gcgcttcacg cgctgttcca gcagggcttc cagctcgtag gaggtatcga agtggttttc caccgcgttc ttcgaggagt gcgtcaccag aacaatctct ttgatcccgg cggcgacgat ttcgtcaacg atgtactgaa tcattggctt atcaacgatc ggcagcatct cctttggaat tgccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHAI PKEMLPIVDK PMIQYIVDEI VAAGIKEIVL VTHSSKNAVE NHFDTSYELE ALLEQRVKRQ LLAEVQAICP PGVTIMNVRQ AQPLGLGHSI LCARPVVGDN PFVVVLPDII LDGGTADPLR YNLAAMIARF NETGRSQVLA KRMPGDLSEY SVIQTKEPMV AEGQVARIVE FIEKPDEPQT LDSDLMAVGR YVLSADIWAE LERTEPGAWG RIQLTDAIAE LAKKPSVDAM LMTGESYDCG KKMGYMQAFV TYGMRNLKEG TKFRESIKKN AGLTAGVKMS GRAEPFGTPL ITLCRNYS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcaattc caaaggagat gctgccgatc gttgataagc caatgattca gtacatcgtt gacgaaatcg tcgccgccgg gatcaaagag attgttctgg tgacgcactc ctcgaagaac gcggtggaaa accacttcga cacatcctac gagctggaag ccctgctgga acagcgcgtg aagcgccagc tgctggcgga agtgcaggcc atttgcccgc cgggcgtgac catcatgaac gtgcgtcagg cgcagccgct gggcctgggc cactccatcc tctgcgcccg cccggtagtc ggcgacaacc cgtttgtggt ggtcctgccg gatatcatcc ttgacggcgg caccgctgac ccgctgcgct ataacctcgc cgctatgatc gcccgcttta acgagaccgg ccgcagccag gtgctggcga aacgcatgcc gggcgatctt tccgaatact ccgtcatcca gaccaaagag ccgatggtgg ctgaaggcca ggtggcgcgt atcgtcgagt ttattgaaaa accggacgag ccgcagactc tggactccga tctgatggcg gtggggcgct acgtgctgtc ggcggatatc tgggccgaac tggaacgtac cgagccgggc gcatggggcc gtatccagct gactgacgcc attgccgaac tggcgaagaa gccgtccgtc gacgcgatgc tgatgaccgg cgaaagctac gactgcggga agaaaatggg ctacatgcag gccttcgtca cctacggcat gcgtaacctg aaagaaggca ccaaattccg cgaaagtatt aaaaaaaatg ctggcctgac agctggcgta aagatgagcg gcagggcaga gcctttcggc acgccgctca taacgttatg cagaaattat tcatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat