SSGCID: Target Page

KlpnC.00116.a

4-hydroxythreonine-4-phosphate dehydrogenase

CENTER ID:	KlpnC.00116.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00116.a.B1.GE44211	Full length(KlpnC.00116.a)		1	329

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.740
UniProt:	A0A0H3GRK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPETRKVVIT PGEPAGIGPD LVVQLAQRDW PVELVICADG ALLTDRAKRL GLPLSLLPYD PAQPPVPQRA GTLTLLNVPL NVPAEPGVLN VQNGAYVVET LARACDGCLS GEFAALVTGP VHKGNINDAG IAFTGHTEFF EERAKASKVV MMLATEELRV ALVTTHLPLK AISEAITPEL LREIITILDH DLRTKFGIAQ PHVLVCGLNP HAGEGGHMGT EEIDTIIPVL EEMRAKGMNL SGPLPADTLF QPKYLDHADA VLAMYHDQGL PVLKYQGFGR GVNITLGLPF IRTSVDHGTA LELAGQGKAD VGSFITALNL AIKMIVNTQ

NT Sequence

ATGCCTGAAA CCCGTAAAGT TGTTATCACT CCCGGCGAAC CCGCCGGGAT TGGCCCTGAC CTGGTCGTGC AGCTTGCCCA GCGCGACTGG CCAGTTGAGC TGGTGATTTG CGCCGATGGC GCCCTGTTAA CTGACCGAGC CAAGCGGCTC GGTCTTCCTT TATCTCTCCT CCCTTACGAT CCCGCGCAAC CTCCTGTTCC TCAGCGCGCC GGTACCCTGA CGCTGCTGAA CGTGCCGCTA AACGTCCCGG CGGAGCCCGG CGTGCTGAAT GTGCAAAACG GCGCCTACGT AGTGGAGACG CTGGCGCGCG CCTGCGACGG CTGCCTTAGC GGCGAGTTTG CGGCGTTAGT GACCGGCCCG GTGCATAAAG GCAACATCAA CGACGCCGGT ATCGCCTTTA CCGGTCATAC CGAGTTCTTT GAAGAGCGCG CGAAGGCCAG CAAAGTGGTG ATGATGTTGG CGACCGAGGA GCTGCGGGTG GCGCTGGTCA CCACCCATCT GCCGTTGAAA GCCATCAGCG AAGCGATTAC CCCCGAGCTA CTGCGCGAGA TCATCACCAT TCTCGATCAC GACCTGCGCA CGAAATTTGG CATTGCCCAG CCGCATGTGC TGGTTTGCGG GCTTAACCCG CACGCTGGCG AAGGCGGCCA TATGGGAACA GAAGAGATAG ACACCATCAT TCCGGTGCTG GAAGAGATGC GCGCAAAGGG GATGAACCTC AGCGGTCCGC TGCCGGCAGA CACCCTCTTT CAGCCGAAAT ATCTTGATCA TGCCGATGCG GTACTCGCGA TGTACCACGA TCAGGGCCTG CCCGTGCTAA AATACCAGGG CTTTGGCCGC GGCGTGAACA TTACGCTCGG TTTACCTTTT ATTCGTACCT CCGTCGACCA CGGCACCGCA CTGGAATTAG CGGGCCAGGG AAAAGCGGAC GTCGGCAGTT TTATCACGGC GCTTAATCTC GCCATCAAAA TGATTGTTAA TACCCAA

Details for KlpnC.00116.a.B1.GE44211

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP ETRKVVITPG EPAGIGPDLV VQLAQRDWPV ELVICADGAL LTDRAKRLGL PLSLLPYDPA QPPVPQRAGT LTLLNVPLNV PAEPGVLNVQ NGAYVVETLA RACDGCLSGE FAALVTGPVH KGNINDAGIA FTGHTEFFEE RAKASKVVMM LATEELRVAL VTTHLPLKAI SEAITPELLR EIITILDHDL RTKFGIAQPH VLVCGLNPHA GEGGHMGTEE IDTIIPVLEE MRTKGMNLSG PLPADTLFQP KYLDHADAVL AMYHDQGLPV LKYQGFGRGV NITLGLPFIR TSVDHGTALE LAGQGKADVG SFITALNL

Validated NT Sequence

gnnagattaa gcgccgtgat aaaactgccg acgtccgctt ttccctggcc cgctaattcc agtgcggtgc cgtggtcgac ggaggtacga ataaaaggta aaccgagcgt aatgttcacg ccgcggccaa agccctggta ttttagcacg ggcaggccct gatcgtggta catcgcgagt accgcatcgg catgatcaag atatttcggc tgaaagaggg tgtctgccgg cagcggaccg ctgaggttca tcccctttgt gcgcatctct tccagcaccg gaatgatggt gtctatctct tctgttccca tatggccgcc ttcgccagcg tgcgggttaa gcccgcaaac cagcacatgc ggctgggcaa tgccaaattt cgtgcgcagg tcgtgatcga gaatggtgat gatctcgcgc agtagctcgg gggtaatcgc ttcgctgatg gctttcaacg gcagatgggt ggtgaccagc gccacccgca gctcctcggt cgccaacatc atcaccactt tgctggcctt cgcgcgctct tcaaagaact cggtatgacc ggtaaaggcg ataccggcgt cgttgatgtt gcctttatgc accgggccgg tcactaacgc cgcaaactcg ccgctaaggc aaccgtcgca ggcgcgcgcc agcgtctcca ctacgtaggc gccgttttgc acattcagca cgccgggctc cgccgggacg tttagcggca cgttcagcag cgtcagggta ccggcgcgct gaggaacagg aggttgcgcg ggatcgtaag ggaggagaga taaaggaaga ccgagccgct tggctcggtc agttaacagg gcgccatcgg cgcaaatcac cagctcaact ggccagtcgc gctgggcaag ctgcacgacc aggtcagggc caatcccggc gggttcgccg ggagtgataa caactttacg ggtttcaggc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMP ETRKVVITPG EPAGIGPDLV VQLAQRDWPV ELVICADGAL LTDRAKRLGL PLSLLPYDPA QPPVPQRAGT LTLLNVPLNV PAEPGVLNVQ NGAYVVETLA RACDGCLSGE FAALVTGPVH KGNINDAGIA FTGHTEFFEE RAKASKVVMM LATEELRVAL VTTHLPLKAI SEAITPELLR EIITILDHDL RTKFGIAQPH VLVCGLNPHA GEGGHMGTEE IDTIIPVLEE MRAKGMNLSG PLPADTLFQP KYLDHADAVL AMYHDQGLPV LKYQGFGRGV NITLGLPFIR TSVDHGTALE LAGQGKADVG SFITALNLAI KMIVNTQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg aaacccgtaa agttgttatc actcccggcg aacccgccgg gattggccct gacctggtcg tgcagcttgc ccagcgcgac tggccagttg agctggtgat ttgcgccgat ggcgccctgt taactgaccg agccaagcgg ctcggtcttc ctttatctct cctcccttac gatcccgcgc aacctcctgt tcctcagcgc gccggtaccc tgacgctgct gaacgtgccg ctaaacgtcc cggcggagcc cggcgtgctg aatgtgcaaa acggcgccta cgtagtggag acgctggcgc gcgcctgcga cggctgcctt agcggcgagt ttgcggcgtt agtgaccggc ccggtgcata aaggcaacat caacgacgcc ggtatcgcct ttaccggtca taccgagttc tttgaagagc gcgcgaaggc cagcaaagtg gtgatgatgt tggcgaccga ggagctgcgg gtggcgctgg tcaccaccca tctgccgttg aaagccatca gcgaagcgat tacccccgag ctactgcgcg agatcatcac cattctcgat cacgacctgc gcacgaaatt tggcattgcc cagccgcatg tgctggtttg cgggcttaac ccgcacgctg gcgaaggcgg ccatatggga acagaagaga tagacaccat cattccggtg ctggaagaga tgcgcgcaaa ggggatgaac ctcagcggtc cgctgccggc agacaccctc tttcagccga aatatcttga tcatgccgat gcggtactcg cgatgtacca cgatcagggc ctgcccgtgc taaaatacca gggctttggc cgcggcgtga acattacgct cggtttacct tttattcgta cctccgtcga ccacggcacc gcactggaat tagcgggcca gggaaaagcg gacgtcggca gttttatcac ggcgcttaat ctcgccatca aaatgattgt taatacccaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat