SSGCID: Target Page

KlpnC.00109.a

Putative cytosine deaminase

CENTER ID:	KlpnC.00109.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00109.a.B1.GE44210	Full length(KlpnC.00109.a)		1	440

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00109.a.B1.PS38689	Full length(KlpnC.00109.a)		1	440

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.4788
UniProt:	A0A0H3GU61

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTAAPLWLIQ NVRLADRDGL WQIAIDKGRF GEITPMGEAH DESYEVLNAR GGLAIPPFIE PHIHLDTTQT AGEPNWNQSG TLFEGIERWA ERKALLSHED VKARAWKTLK WQIANGVQFV RTHVDVSDPT LTALKAMLEV KQEVAPWVEL QIVAFPQEGI LSYPNGEALL EEALKLGADV VGAIPHFEFT REYGVESLHI AFRLAQQYDR PLDIHCDEID DEQSRFVETV AALALKAGIG PRVTASHTTA MHSYNGAYTS RLFRLLKLSG INFVANPLVN IHLQGRFDDY PKRRGITRVK ELLAAGINVC FGHDDVFDPW YPLGTGNMLQ VLHMGLHVCQ LMGYQQINDG LQLITDNSAR TFGLEDYGIV SGNPANLIIL PAENGFEAVR CQVPVRWSIR QGRVIATTQP AQSWIQTDRG GEELSFMRNS PLADAKGPKA

NT Sequence

ATGACAGCAG CGCCGCTTTG GTTGATTCAG AACGTACGCC TGGCAGACCG CGACGGCCTG TGGCAAATAG CCATTGATAA GGGCCGCTTT GGCGAGATCA CGCCGATGGG GGAGGCGCAC GATGAAAGCT ACGAGGTATT GAACGCTCGC GGCGGGCTAG CGATCCCGCC TTTTATCGAG CCGCATATCC ATCTGGATAC CACCCAGACG GCCGGCGAGC CGAACTGGAA TCAGTCCGGT ACTCTGTTCG AGGGGATTGA GCGCTGGGCG GAGCGGAAAG CGCTGCTCAG CCATGAGGAC GTCAAAGCCC GGGCGTGGAA AACCCTGAAG TGGCAGATCG CCAATGGCGT GCAGTTTGTC CGCACTCATG TCGATGTTTC CGATCCCACG TTAACGGCGC TGAAGGCGAT GCTGGAGGTC AAACAGGAGG TGGCGCCATG GGTCGAGTTA CAGATTGTCG CTTTCCCACA GGAGGGGATC CTCTCTTACC CCAACGGCGA GGCGCTGCTG GAGGAGGCCC TCAAGCTGGG GGCGGACGTC GTCGGGGCGA TCCCGCATTT TGAGTTCACC CGTGAATATG GTGTGGAATC GCTGCATATC GCCTTCCGTC TGGCGCAGCA ATATGACCGC CCACTGGATA TTCACTGCGA TGAAATTGAC GATGAGCAGT CGCGCTTTGT TGAAACCGTT GCCGCGCTGG CGCTGAAAGC GGGGATAGGG CCGCGGGTAA CGGCCAGCCA TACCACCGCT ATGCACTCCT ATAATGGCGC TTATACCTCG CGTCTGTTCC GCTTGCTTAA GCTCTCCGGC ATCAACTTTG TCGCCAATCC GCTGGTGAAT ATTCATCTGC AGGGCCGGTT TGACGACTAC CCGAAGCGAC GGGGCATTAC CCGGGTGAAG GAGCTGTTGG CGGCGGGGAT CAACGTTTGC TTTGGTCATG ATGACGTCTT TGACCCGTGG TACCCGCTGG GGACCGGCAA TATGCTGCAG GTCCTGCATA TGGGGCTCCA TGTTTGCCAG CTGATGGGCT ACCAGCAGAT CAACGACGGC CTGCAGCTTA TCACCGACAA CAGCGCCCGC ACGTTTGGCC TCGAGGATTA CGGCATTGTC AGCGGCAACC CGGCTAACCT GATCATTCTC CCGGCGGAGA ATGGCTTTGA GGCCGTCCGC TGCCAGGTGC CGGTGCGCTG GTCAATTCGC CAGGGAAGGG TGATCGCGAC CACCCAGCCG GCGCAAAGCT GGATCCAAAC TGACCGCGGT GGCGAGGAGC TTTCTTTTAT GAGGAACAGC CCCCTTGCGG ATGCAAAGGG GCCGAAGGCT

Details for KlpnC.00109.a.B1.GE44210

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	96

Validated AA Sequence

MTAAPLWLIQ NVRLADRDGL WQIAIDKGRF GAITPMGEAH DESYEVLNAR GGLAIPPXXX PHIHLDTTQT AGEPNWNQSG TLFEGIERWA ERKALLSHED VKARAWKTLX XXIANGVQFV RTHVDVSDPT LTALKAMLEV KQEVAPWVEL QXVAFPQEGI LSYPNGEALL EEALKLGADV VGAIPHFEFT REYGVESLHI AFRLAQQYDR PLDIHCDEID DEQSRFVETV AALALKAGIG PRVTASHTTA MHSYNGAYTS RLFRLLKLSG INFVANPLVN IHLQGRFDDY PKRRGITRVK ELLAAGINVC FGHDDVFDPW YPLGTGNMLQ VLHMGLHVCQ LMGYQQINDG LQLITDNSTR TFGLEDYGIV SGNPANLIIL PAENGFEAVR CQVPVRWSIR QGRVIATTQP AQSWIQTDRG GEELSFMRNS P

Validated NT Sequence

aagggggctg ttcctcataa aagaaagctc ctcgccaccg cggtcagttt ggatccagct ttgcgccggc tgggtggtcg cgatcaccct tccctggcga attgaccagc gcaccggcac ctggcagcgg acggcctcaa agccattctc cgccgggaga atgatcaggt tagccgggtt gccgctgaca atgccgtaat cctcgaggcc aaacgtgcgg gtgctgttgt cggtgataag ctgcaggccg tcgttgatct gctggtagcc catcagctgg caaacatgga gccccatatg caggacctgc agcatattgc cggtccccag cgggtaccac gggtcaaaga cgtcatcatg accaaagcaa acgttgatcc ccgccgccaa cagctccttc acccgggtaa tgccccgtcg cttcgggtag tcgtcaaacc ggccctgcag atgaatattc accagcggat tggcgacaaa gttgatgccg gagagcttaa gcaagcggaa cagacgcgag gtataagcgc cattatagga gtgcatagcg gtggtatggc tggccgttac ccgcggccct atccccgctt tcagcgccag cgcggcaacg gtttcaacaa agcgcgactg ctcatcgtca atttcatcgc agtgaatatc cagtgggcgg tcatattgct gcgccagacg gaaggcgata tgcagcgatt ccacaccata ttcacgggtg aactcaaaat gcgggatcgc cccgacgacg tccgccccca gcttgagggc ctcctccagc agcgcctcgc cgttggggta agagaggatc ccctcctgtg gaaaagcgac antctgtaac tcgacccatg gcgccacctc ctgtttgacc tccagcatcg ccttcagcgc cgttaacgtg ggatcggaaa catcgacatg agtgcggaca aactgcacgc cattggcgat ctnncnnttc agggttttcc acgcccgggc tttgacgtcc tcatggctga gcagcgcttt ccgctccgcc cagcgctcaa tcccctcgaa cagagtaccg gactgattcc agttcggctc gccggccgtc tgggtggtat ccagatggat atgcggctnn nnnnaaggcg ggatcgctag cccgccgcga gcgttcaata cctcatagct ttcatcgtgc gcctccccca tcggcgtgat cgcgccaaag cggcccttat caatggctat ttgccacagg ccgtcgcggt ctgccaggcg tacgttctga atcaaccaaa gcggcgctgc tgtcatatgg tggtggnggn gnnnnnnc

Expected Protein Sequence

MAHHHHHHMT AAPLWLIQNV RLADRDGLWQ IAIDKGRFGE ITPMGEAHDE SYEVLNARGG LAIPPFIEPH IHLDTTQTAG EPNWNQSGTL FEGIERWAER KALLSHEDVK ARAWKTLKWQ IANGVQFVRT HVDVSDPTLT ALKAMLEVKQ EVAPWVELQI VAFPQEGILS YPNGEALLEE ALKLGADVVG AIPHFEFTRE YGVESLHIAF RLAQQYDRPL DIHCDEIDDE QSRFVETVAA LALKAGIGPR VTASHTTAMH SYNGAYTSRL FRLLKLSGIN FVANPLVNIH LQGRFDDYPK RRGITRVKEL LAAGINVCFG HDDVFDPWYP LGTGNMLQVL HMGLHVCQLM GYQQINDGLQ LITDNSARTF GLEDYGIVSG NPANLIILPA ENGFEAVRCQ VPVRWSIRQG RVIATTQPAQ SWIQTDRGGE ELSFMRNSPL ADAKGPKA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag cagcgccgct ttggttgatt cagaacgtac gcctggcaga ccgcgacggc ctgtggcaaa tagccattga taagggccgc tttggcgaga tcacgccgat gggggaggcg cacgatgaaa gctacgaggt attgaacgct cgcggcgggc tagcgatccc gccttttatc gagccgcata tccatctgga taccacccag acggccggcg agccgaactg gaatcagtcc ggtactctgt tcgaggggat tgagcgctgg gcggagcgga aagcgctgct cagccatgag gacgtcaaag cccgggcgtg gaaaaccctg aagtggcaga tcgccaatgg cgtgcagttt gtccgcactc atgtcgatgt ttccgatccc acgttaacgg cgctgaaggc gatgctggag gtcaaacagg aggtggcgcc atgggtcgag ttacagattg tcgctttccc acaggagggg atcctctctt accccaacgg cgaggcgctg ctggaggagg ccctcaagct gggggcggac gtcgtcgggg cgatcccgca ttttgagttc acccgtgaat atggtgtgga atcgctgcat atcgccttcc gtctggcgca gcaatatgac cgcccactgg atattcactg cgatgaaatt gacgatgagc agtcgcgctt tgttgaaacc gttgccgcgc tggcgctgaa agcggggata gggccgcggg taacggccag ccataccacc gctatgcact cctataatgg cgcttatacc tcgcgtctgt tccgcttgct taagctctcc ggcatcaact ttgtcgccaa tccgctggtg aatattcatc tgcagggccg gtttgacgac tacccgaagc gacggggcat tacccgggtg aaggagctgt tggcggcggg gatcaacgtt tgctttggtc atgatgacgt ctttgacccg tggtacccgc tggggaccgg caatatgctg caggtcctgc atatggggct ccatgtttgc cagctgatgg gctaccagca gatcaacgac ggcctgcagc ttatcaccga caacagcgcc cgcacgtttg gcctcgagga ttacggcatt gtcagcggca acccggctaa cctgatcatt ctcccggcgg agaatggctt tgaggccgtc cgctgccagg tgccggtgcg ctggtcaatt cgccagggaa gggtgatcgc gaccacccag ccggcgcaaa gctggatcca aactgaccgc ggtggcgagg agctttcttt tatgaggaac agcccccttg cggatgcaaa ggggccgaag gcttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for KlpnC.00109.a.B1.PS38689

PURIFICATION DATe:	8/18/2021
CONCENTRATION:	30.23mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAG CAGCGCCGCT TTGGTTGATT CAGAACGTAC GCCTGGCAGA CCGCGACGGC CTGTGGCAAA TAGCCATTGA TAAGGGCCGC TTTGGCGAGA TCACGCCGAT GGGGGAGGCG CACGATGAAA GCTACGAGGT ATTGAACGCT CGCGGCGGGC TAGCGATCCC GCCTTTTATC GAGCCGCATA TCCATCTGGA TACCACCCAG ACGGCCGGCG AGCCGAACTG GAATCAGTCC GGTACTCTGT TCGAGGGGAT TGAGCGCTGG GCGGAGCGGA AAGCGCTGCT CAGCCATGAG GACGTCAAAG CCCGGGCGTG GAAAACCCTG AAGTGGCAGA TCGCCAATGG CGTGCAGTTT GTCCGCACTC ATGTCGATGT TTCCGATCCC ACGTTAACGG CGCTGAAGGC GATGCTGGAG GTCAAACAGG AGGTGGCGCC ATGGGTCGAG TTACAGATTG TCGCTTTCCC ACAGGAGGGG ATCCTCTCTT ACCCCAACGG CGAGGCGCTG CTGGAGGAGG CCCTCAAGCT GGGGGCGGAC GTCGTCGGGG CGATCCCGCA TTTTGAGTTC ACCCGTGAAT ATGGTGTGGA ATCGCTGCAT ATCGCCTTCC GTCTGGCGCA GCAATATGAC CGCCCACTGG ATATTCACTG CGATGAAATT GACGATGAGC AGTCGCGCTT TGTTGAAACC GTTGCCGCGC TGGCGCTGAA AGCGGGGATA GGGCCGCGGG TAACGGCCAG CCATACCACC GCTATGCACT CCTATAATGG CGCTTATACC TCGCGTCTGT TCCGCTTGCT TAAGCTCTCC GGCATCAACT TTGTCGCCAA TCCGCTGGTG AATATTCATC TGCAGGGCCG GTTTGACGAC TACCCGAAGC GACGGGGCAT TACCCGGGTG AAGGAGCTGT TGGCGGCGGG GATCAACGTT TGCTTTGGTC ATGATGACGT CTTTGACCCG TGGTACCCGC TGGGGACCGG CAATATGCTG CAGGTCCTGC ATATGGGGCT CCATGTTTGC CAGCTGATGG GCTACCAGCA GATCAACGAC GGCCTGCAGC TTATCACCGA CAACAGCGCC CGCACGTTTG GCCTCGAGGA TTACGGCATT GTCAGCGGCA ACCCGGCTAA CCTGATCATT CTCCCGGCGG AGAATGGCTT TGAGGCCGTC CGCTGCCAGG TGCCGGTGCG CTGGTCAATT CGCCAGGGAA GGGTGATCGC GACCACCCAG CCGGCGCAAA GCTGGATCCA AACTGACCGC GGTGGCGAGG AGCTTTCTTT TATGAGGAAC AGCCCCCTTG CGGATGCAAA GGGGCCGAAG GCTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT